Isei Tanida

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. doi Autophagy basics
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Shinjyuku, Tokyo, Japan
    Microbiol Immunol 55:1-11. 2011
  2. pmc Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons
    Masato Koike
    Department of Cell Biology and Neuroscience, Juntendo University School of Medicine, Tokyo, Japan
    PLoS ONE 8:e63568. 2013
  3. doi The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    Autophagy 8:88-97. 2012
  4. ncbi Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Shinjyuku, Tokyo, Japan
    Autophagy 5:937-45. 2009
  5. doi Autophagosome formation and molecular mechanism of autophagy
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Shinjyuku, Japan
    Antioxid Redox Signal 14:2201-14. 2011
  6. ncbi Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16
    Yu Shin Sou
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Biol Chem 281:3017-24. 2006
  7. ncbi Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 292:256-62. 2002
  8. ncbi HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Biol Chem 279:36268-76. 2004
  9. ncbi Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3
    Isei Tanida
    Molecular Cell Biology, Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan
    FEBS J 273:2553-62. 2006
  10. ncbi GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 300:637-44. 2003

Collaborators

Detail Information

Publications29

  1. doi Autophagy basics
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Shinjyuku, Tokyo, Japan
    Microbiol Immunol 55:1-11. 2011
    ..In this review, the relationships between these Atg complexes and each process are described. Finally, the critical points for monitoring autophagy, including the use of GFP-LC3 and GFP-Atg5, are discussed...
  2. pmc Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons
    Masato Koike
    Department of Cell Biology and Neuroscience, Juntendo University School of Medicine, Tokyo, Japan
    PLoS ONE 8:e63568. 2013
    ..These results indicated that, compared with LC3, GABARAP is enriched in the AIS, in addition to the cell bodies and dendrites, of these hippocampal pyramidal neurons and cerebellar Purkinje cells...
  3. doi The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    Autophagy 8:88-97. 2012
    ..These results indicated that the FAP motif of ATG7 is indispensable for formation of the ATG3-LC3 E2-substrate intermediate through the interaction of ATG7 with ATG3...
  4. ncbi Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Shinjyuku, Tokyo, Japan
    Autophagy 5:937-45. 2009
    ....
  5. doi Autophagosome formation and molecular mechanism of autophagy
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Shinjyuku, Japan
    Antioxid Redox Signal 14:2201-14. 2011
    ..Processes of selective autophagy require specific Atg proteins in addition to the "core" Atg complexes. Finally, methods to monitor autophagic activity in mammalian cells are described...
  6. ncbi Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16
    Yu Shin Sou
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Biol Chem 281:3017-24. 2006
    ..The discrepancy between in vitro and in vivo reactions suggested that there may be selective factor(s) involved in the endogenous LC3 conjugation system...
  7. ncbi Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 292:256-62. 2002
    ..We also report here on the ubiquitous expression of human APG7 mRNA in human adult and fetal tissues and of rat Apg7p in adult tissues...
  8. ncbi HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Biol Chem 279:36268-76. 2004
    ..RNA interference of HsAtg4B increased the amount of LC3-PL in HEK293 cells. Taken together, these results suggest that HsAtg4B negatively regulates the localization of LC3 to a membrane compartment by delipidation...
  9. ncbi Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3
    Isei Tanida
    Molecular Cell Biology, Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan
    FEBS J 273:2553-62. 2006
    ..However, little accumulation of the modified form of Atg8L was observed under conditions of starvation. These results indicate that Atg8L is the fourth modifier of mammalian Atg8 conjugation...
  10. ncbi GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 300:637-44. 2003
    ..These results indicate that GATE-16 and GABARAP are authentic modifiers, but that they have different biochemical characteristics from those of LC3...
  11. ncbi Lysosomal turnover of GABARAP-phospholipid conjugate is activated during differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan
    Autophagy 2:264-71. 2006
    ....
  12. ncbi Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Biol Chem 279:47704-10. 2004
    ..RNA interference of MAP1LC3B mRNA resulted in a decrease in both endogenous MAP1LC3B-PL and MAP1LC3B. These results indicate that the carboxyl terminus of MAP1LC3B is cleaved to expose Gly(120) for further ubiquitylation-like reactions...
  13. ncbi Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, 113 8421, Tokyo, Japan
    Biochem Biophys Res Commun 296:1164-70. 2002
    ..These results indicate that both Apg12p and the Apg12p.Apg5p conjugate are regulatory factors for LC3 processing...
  14. pmc A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier
    Masaaki Komatsu
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo, Japan
    EMBO J 23:1977-86. 2004
    ..Ufm1, Uba5, and Ufc1 are all conserved in metazoa and plants but not in yeast, suggesting its potential roles in various multicellular organisms...
  15. ncbi Characterization of CAA0225, a novel inhibitor specific for cathepsin L, as a probe for autophagic proteolysis
    Katsuyuki Takahashi
    Clinical Laboratory Department, Nihon University School of Medicine, Tokyo, Japan
    Biol Pharm Bull 32:475-9. 2009
    ..Therefore we conclude that cathepsin L does not play a general role in the degradation of proteins in the lumen of autophagosomes, but rather is involved specifically in the degradation of autophagosomal membrane markers...
  16. doi Doxorubicin-induced glomerulosclerosis with proteinuria in GFP-GABARAP transgenic mice
    Miyuki Takagi-Akiba
    Div of Nephrology, Dept of Internal Medicine, Juntendo Univ, Hongo, Bunkyo ku, Tokyo, Japan
    Am J Physiol Renal Physiol 302:F380-9. 2012
    ..These results indicate that the GFP-GABARAP/p62 complex is responsible for impairment of glomerular function and that it retards recovery from the effects of doxorubicin...
  17. ncbi Excess peroxisomes are degraded by autophagic machinery in mammals
    Jun ichi Iwata
    Department of Biochemistry, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    J Biol Chem 281:4035-41. 2006
    ..This is the first direct evidence for the contribution of autophagic machinery in peroxisomal degradation in mammals...
  18. doi Autophagic compartments gain access to the MHC class II compartments in thymic epithelium
    Michiyuki Kasai
    Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Musashimurayama shi, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Immunol 183:7278-85. 2009
    ..These results strongly suggest that the cytoplasmic self-Ags gain access to the H2-DM-positive compartments via the autophagic process in the thymus...
  19. pmc Liver autophagy contributes to the maintenance of blood glucose and amino acid levels
    Junji Ezaki
    Department of Biochemistry, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    Autophagy 7:727-36. 2011
    ..This indicates that insulin plays a dominant role over glucagon in controlling liver autophagy. These results are the first to show that liver-specific autophagy plays a role in blood glucose regulation...
  20. ncbi Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan
    Autophagy 1:84-91. 2005
    ....
  21. ncbi Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, 2 1 1, Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Biol Chem 277:13739-44. 2002
    ..Furthermore, hApg3p coimmunoprecipitates with hApg12p, and the overexpression of hApg3p facilitates the formation of the GFPhApg12p.thApg5p conjugate, suggesting that hApg3p cross-talks with the hApg12p conjugation system...
  22. ncbi Loss of Pten, a tumor suppressor, causes the strong inhibition of autophagy without affecting LC3 lipidation
    Takashi Ueno
    The Department of Biochemistry, Juntendo University School of Medicine, Hongo, Tokyo, Japan
    Autophagy 4:692-700. 2008
    ..Collectively, we conclude that enhanced insulin signaling in Pten deficiency suppresses autophagy at the formation and maturation steps of autophagosomes, without inhibiting ATG conjugation reactions...
  23. ncbi Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Disease, Tokyo, Japan
    Autophagy 4:131-4. 2008
    ..These mutant LC3DeltaG proteins will be useful negative controls in recognizing non-specific fluorescent protein-LC3 aggregates...
  24. doi LC3 and Autophagy
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    Methods Mol Biol 445:77-88. 2008
    ..Here we describe basic protocols to assay for endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence...
  25. doi Hepatic steatosis inhibits autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression
    Yoshihiro Inami
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Biochem Biophys Res Commun 412:618-25. 2011
    ..These results indicate that lipid accumulation blunts autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression...
  26. ncbi MAP-LC3, a promising autophagosomal marker, is processed during the differentiation and recovery of podocytes from PAN nephrosis
    Katsuhiko Asanuma
    Division of Nephrology, Department of Internal Medicine, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    FASEB J 17:1165-7. 2003
    ..These results suggest that LC3-II-localized vesicles play an important role in the physiological function of podocytes...
  27. ncbi The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation
    Harumi Yamazaki-Sato
    Department of Biochemistry, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, 113 8421 Tokyo, Japan
    FEBS Lett 551:71-7. 2003
    ..These results suggest that the carboxyl terminal 17 amino acids of Apg7 play a specific role in Apg8 lipidation indispensable for the Cvt pathway and autophagy...
  28. doi Synthetic fibril peptide promotes clearance of scrapie prion protein by lysosomal degradation
    Yuko Okemoto-Nakamura
    Department of Biochemistry and Cell Biology, National Institue of Infectious Diseases, Tokyo, Japan
    Microbiol Immunol 52:357-65. 2008
    ..These results indicate that P9 promotes PrP(Sc) redistribution from late endosomes to lysosomes, thereby attaining PrP(Sc) degradation...
  29. pmc Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition
    Shinji Saiki
    Department of Neurology, Juntendo University School of Medicine Bunkyo, Tokyo, Japan
    Autophagy 7:176-87. 2011
    ..These results support previous studies on the use of caffeine in the treatment of human tumors and indicate a potential new target in the regulation of apoptosis...