Research Topics
Genomes and Genes | J ShimaSummaryAffiliation: National Institute of Agrobiological Sciences Country: Japan Publications
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Detail Information
Publications
Stress tolerance in doughs of Saccharomyces cerevisiae trehalase mutants derived from commercial Baker's yeastJ Shima
National Food Research Institute, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
Appl Environ Microbiol 65:2841-6. 1999..The improved freeze tolerance exhibited by all of the trehalase mutants may make these strains useful in frozen dough...
Identification of genes whose expressions are enhanced or reduced in baker's yeast during fed-batch culture process using molasses medium by DNA microarray analysisJun Shima
National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
Int J Food Microbiol 102:63-71. 2005..The genes involved in the metabolism of amino acids also showed enhanced expression in synthetic medium. This identification of genes provides information that will help improve the baker's yeast production process...
Disruption of the CAR1 gene encoding arginase enhances freeze tolerance of the commercial baker's yeast Saccharomyces cerevisiaeJun Shima
National Food Research Institute, Tsukuba, Ibaraki 305 8642, Japan
Appl Environ Microbiol 69:715-8. 2003..An arginase mutant accumulated higher levels of arginine and/or glutamate and showed increased leavening ability during the frozen-dough baking process, suggesting that disruption of the CAR1 gene enhances freeze tolerance...
EOS1, whose deletion confers sensitivity to oxidative stress, is involved in N-glycosylation in Saccharomyces cerevisiaeToshihide Nakamura
National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
Biochem Biophys Res Commun 353:293-8. 2007..The inhibition of the N-glycosylation of carboxypeptidase Y and invertase activity caused by the addition of tunicamycin was depressed in Delta eos1, suggesting that EOS1 may be involved in N-glycosylation of the cellular proteins...
Isolation and characterization of a low molecular weight peptide contained in sourdoughToshihide Nakamura
National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
J Agric Food Chem 55:4871-6. 2007..SDP1 showed angiotensin-converting enzyme (ACE) inhibitory activity, and the 50% inhibitory peptide concentration (IC50) was 336 microM. It is possible that the SDP1 peptide partially confers ACE inhibitory activity in sourdough...
Functional genomics of commercial baker's yeasts that have different abilities for sugar utilization and high-sucrose tolerance under different sugar conditionsFumiko Tanaka Tsuno
National Food Research Institute, 2 1 12, Kannondai Tsukuba, Ibaraki 305 8642, Japan
Yeast 24:901-11. 2007..Welch's t-test for this comparison showed that the relative growth rates of the deletion strains whose deletion occurred in genes belonging to cluster 1 were significantly higher than the average growth rates of all deletion strains...
Overexpression of two transcriptional factors, Kin28 and Pog1, suppresses the stress sensitivity caused by the rsp5 mutation in Saccharomyces cerevisiaeMika Demae
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
FEMS Microbiol Lett 277:70-8. 2007..These results suggest that the overexpression of Kin28 and Pog1 enhances the protein refolding and degradation pathways in rsp5(A401E) cells...
Possible roles of vacuolar H+-ATPase and mitochondrial function in tolerance to air-drying stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strainsJun Shima
National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
Yeast 25:179-90. 2008..The results suggested that oxidative stress is a critical determinant of sensitivity to air-drying stress, although ROS-scavenging systems are not necessary for air-drying stress tolerance...
Characterization of glutamate decarboxylase from a high gamma-aminobutyric acid (GABA)-producer, Lactobacillus paracaseiNoriko Komatsuzaki
National Food Research Institute, Tsukuba, Ibaraki 305 8642, Japan
Biosci Biotechnol Biochem 72:278-85. 2008..The findings suggest that the ability of Lb. paracasei to produce high levels of GABA results from two characteristics of GAD, viz., a low Km value and activity at low pH...
Identification and classification of genes required for tolerance to freeze-thaw stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strainsAkira Ando
National Food Research Institute, Tsukuba, Ibaraki, Japan
FEMS Yeast Res 7:244-53. 2007..Our results suggest the presence of at least two different mechanisms of freeze-thaw injury: oxidative stress generated during the freeze-thaw process, and defects in cell wall assembly...
Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentationFumiko Tanaka
National Food Research Institute, Tsukuba, Ibaraki, Japan
Food Microbiol 23:717-28. 2006..Our study presents the first overall description of the transcriptional response of baker's yeast during dough-fermentation, and will thus help clarify genomic responses to various stresses during commercial fermentation processes...
Identification and classification of genes required for tolerance to high-sucrose stress revealed by genome-wide screening of Saccharomyces cerevisiaeAkira Ando
National Food Research Institute, Ibaraki, Japan
FEMS Yeast Res 6:249-67. 2006..The genes identified in this study might be important for tolerance to high-sucrose stress, and therefore should be target genes in future research into molecular modification for breeding of yeast tolerant to high-sucrose stress...
Survival of genetically modified and self-cloned strains of commercial baker's yeast in simulated natural environments: environmental risk assessmentAkira Ando
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
Appl Environ Microbiol 71:7075-82. 2005..In conclusion, disruption of ATH1 by genetic engineering apparently does not promote the survival of viable cells and DNA in natural environments...
Superior molasses assimilation, stress tolerance, and trehalose accumulation of baker's yeast isolated from dried sweet potatoes (hoshi-imo)Osamu Nishida
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
Biosci Biotechnol Biochem 68:1442-8. 2004..These results suggest that ONY1 has potential commercial use as baker's yeast for frozen dough and high-sugar dough...
Biochemical and genetic characterization of mundticin KS, an antilisterial peptide produced by Enterococcus mundtii NFRI 7393Shinichi Kawamoto
National Food Research Institute, Tsukuba, Ibaraki 305 8642, Japan
Appl Environ Microbiol 68:3830-40. 2002..faecium, L. curvatus, and Lactococcus lactis. Based on our results, the mun locus is located on a 50-kb plasmid, pML1, of E. mundtii NFRI 7393...
Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughsTomohiro Kaino
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
Appl Environ Microbiol 74:5845-9. 2008..These results suggest that proline-accumulating baker's yeast is suitable for frozen-dough baking...
Isolation of thermophilic ammonium-tolerant bacterium and its application to reduce ammonia emission during composting of animal wastesKazutaka Kuroda
National Institute of Livestock and Grassland Science, National Agriculture and Bio-oriented Research Organization, Tukuba, Ibaraki, Japan
Biosci Biotechnol Biochem 68:286-92. 2004..These results suggested the possibility of reducing NH3 emission from composting of animal wastes by adding TAT105...
Characterization of Enterococcus faecium mutants resistant to mundticin KS, a class IIa bacteriocinYouko Sakayori
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
Microbiology 149:2901-8. 2003..These changes in membrane structure may influence resistance of enterococci to class IIa and class I bacteriocins...
The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathwaysChao Wen Wang
Department of Molecular, Cellular, and Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
J Biol Chem 277:47917-27. 2002....
