Hiroyuki Sasaki

Summary

Affiliation: National Institute of Genetics
Country: Japan

Publications

  1. ncbi Mechanisms of Igf2/H19 imprinting: DNA methylation, chromatin and long-distance gene regulation
    H Sasaki
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Yata, Mishima, Shizuoka 411 8540, Japan
    J Biochem 127:711-5. 2000
  2. ncbi Tsix defective in splicing is competent to establish Xist silencing
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, 411 8540, Japan
    Development 133:4925-31. 2006
  3. pmc Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution
    Layla Parker-Katiraee
    Program in Genetics and Genomic Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
    PLoS Genet 3:e65. 2007
  4. pmc Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development
    Ryutaro Hirasawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Genes Dev 22:1607-16. 2008
  5. ncbi [Genomic imprinting, X chromosome inactivation and DNA methylation]
    Hiroyuki Sasaki
    Tanpakushitsu Kakusan Koso 47:1822-8. 2002
  6. doi Epigenetic events in mammalian germ-cell development: reprogramming and beyond
    Hiroyuki Sasaki
    Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, School of Life Science, The Graduate University for Advanced Studies, Yata, Mishima, Japan
    Nat Rev Genet 9:129-40. 2008
  7. doi De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:768-74. 2008
  8. pmc Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus
    Toshiaki Watanabe
    Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Science 332:848-52. 2011
  9. doi A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse
    Yuko Hoki
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima 411 8540, Japan
    Development 136:139-46. 2009
  10. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011

Detail Information

Publications63

  1. ncbi Mechanisms of Igf2/H19 imprinting: DNA methylation, chromatin and long-distance gene regulation
    H Sasaki
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Yata, Mishima, Shizuoka 411 8540, Japan
    J Biochem 127:711-5. 2000
    ..The Igf2/H19 region provides a unique model in which to study the roles of DNA methylation and chromatin structure in the regulation of chromosome domains...
  2. ncbi Tsix defective in splicing is competent to establish Xist silencing
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, 411 8540, Japan
    Development 133:4925-31. 2006
    ..Moreover, the repressive chromatin configuration was properly established at the Xist locus. These unexpected results indicate that the splicing products are dispensable for Tsix-mediated Xist silencing...
  3. pmc Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution
    Layla Parker-Katiraee
    Program in Genetics and Genomic Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
    PLoS Genet 3:e65. 2007
    ..Thus, KLF14 may be the first example of an imprinted transcript undergoing accelerated evolution in the human lineage...
  4. pmc Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development
    Ryutaro Hirasawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Genes Dev 22:1607-16. 2008
    ..Consistent with these results we find that zygotic Dnmt1 is expressed in the preimplantation embryo. Thus, contrary to the previous reports, Dnmt1 alone is sufficient to maintain the methylation marks of the imprinted genes...
  5. ncbi [Genomic imprinting, X chromosome inactivation and DNA methylation]
    Hiroyuki Sasaki
    Tanpakushitsu Kakusan Koso 47:1822-8. 2002
  6. doi Epigenetic events in mammalian germ-cell development: reprogramming and beyond
    Hiroyuki Sasaki
    Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, School of Life Science, The Graduate University for Advanced Studies, Yata, Mishima, Japan
    Nat Rev Genet 9:129-40. 2008
    ..Understanding epigenetic regulation in germ cells has implications for reproductive engineering technologies and human health...
  7. doi De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:768-74. 2008
    ..This underscores the difference between imprinted XCI and autosomal imprinting...
  8. pmc Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus
    Toshiaki Watanabe
    Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Science 332:848-52. 2011
    ..A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1...
  9. doi A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse
    Yuko Hoki
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima 411 8540, Japan
    Development 136:139-46. 2009
    ....
  10. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011
    ..Our results indicate a conserved role for MITOPLD/Zuc in the piRNA pathway and link mitochondrial membrane metabolism/signaling to small RNA biogenesis...
  11. ncbi Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse
    Yuzuru Kato
    Department of Integrated Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan
    Hum Mol Genet 16:2272-80. 2007
    ..Finally, all these sequences showed moderate to severe hypomethylation in Dnmt3L-deficient prospermatogonia, indicating the critical function and broad specificity of this factor in de novo methylation...
  12. ncbi Large-scale identification and mapping of nuclear matrix-attachment regions in the distal imprinted domain of mouse chromosome 7
    Wahyu Purbowasito
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    DNA Res 11:391-407. 2004
    ..This study presents the first large-scale mapping of MARs in an imprinted domain and provides a platform for understanding the roles of MARs in imprinting...
  13. doi De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:spc one. 2008
    ..The study by Chiba et al. in this issue suggests that de novo DNA methyltransferases are dispensable for setting the imprint on the maternally-derived X chromsome in growing oocytes. See Chiba et al. in this issue...
  14. pmc Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes
    Shin Ichi Tomizawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Japan
    Development 138:811-20. 2011
    ..These results provide evidence for a unique methylation profile in oocytes and reveal the surprisingly dynamic nature of DMRs in the early embryo...
  15. ncbi A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7
    Hisao Shirohzu
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima 411 8540, Japan
    DNA Res 11:325-34. 2004
    ..We propose that this repeat segment could serve either as a boundary between the two subdomains or as a target for epigenetic chromatin modifications that regulate imprinting...
  16. doi Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 453:539-43. 2008
    ..Our results reveal a role for endogenous siRNAs in mammalian oocytes and show that organisms lacking RdRP activity can produce functional endogenous siRNAs from naturally occurring dsRNAs...
  17. ncbi Tsix silences Xist through modification of chromatin structure
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, Japan
    Dev Cell 9:159-65. 2005
    ..Moreover, we show that disruption of Tsix impairs establishment of repressive epigenetic modifications and chromatin structure at the Xist locus. We propose that Tsix silences Xist through modification of the chromatin structure...
  18. ncbi Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting
    Masahiro Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 429:900-3. 2004
    ..These results indicate that both Dnmt3a and Dnmt3L are required for methylation of most imprinted loci in germ cells, but also suggest the involvement of other factors...
  19. ncbi Meiotic and epigenetic aberrations in Dnmt3L-deficient male germ cells
    Kenichiro Hata
    Department of Integrated Genetics, Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems ROIS, Mishima, Japan
    Mol Reprod Dev 73:116-22. 2006
    ..These findings indicate that Dnmt3L regulates germ cell-specific gene expression and IAP suppression, which are critical for male germ cell proliferation and meiosis...
  20. pmc Structural and functional analysis of a 0.5-Mb chicken region orthologous to the imprinted mammalian Ascl2/Mash2-Igf2-H19 region
    Takaaki Yokomine
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Genome Res 15:154-65. 2005
    ..Our findings show that the imprinted genes were clustered before the emergence of imprinting and that the elements associated with imprinting probably evolved after the divergence of mammals and birds...
  21. doi Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos
    Ryutaro Hirasawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Gene Expr Patterns 9:27-30. 2009
    ..The findings are consistent with the role for Dnmt3b in cell-lineage specification and the creation of lineage-specific DNA methylation patterns...
  22. ncbi An evolutionarily conserved putative insulator element near the 3' boundary of the imprinted Igf2/H19 domain
    Ko Ishihara
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Graduate University for Advanced Studies, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Hum Mol Genet 11:1627-36. 2002
    ..Together with the findings on the differentially methylated CTCF sites 5' to H19, CTCF-dependent insulators may not only regulate but also delimit the imprinted domain...
  23. ncbi Imprinting and looping: epigenetic marks control interactions between regulatory elements
    Yuzuru Kato
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Bioessays 27:1-4. 2005
    ..The study gives us a novel view on how regulatory elements influence gene expression and how epigenetic modifications modulate their long-range effects...
  24. pmc Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
    Toshiaki Watanabe
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Genes Dev 20:1732-43. 2006
    ..These features are reminiscent of Tetrahymena approximately 23- to 24-nt small RNAs and Caenorhabditis elegans X-cluster small RNAs. A conserved novel small RNA pathway may be present in diverse animals...
  25. ncbi Regulation of imprinted X-chromosome inactivation in mice by Tsix
    T Sado
    Division of Human Genetics, National Institute of Genetics, Yata, Mishima, Japan
    Development 128:1275-86. 2001
    ..These results provide genetic evidence that Tsix plays a crucial role in maintaining Xist silencing in cis and in regulation of imprinted X-inactivation in the extra-embryonic tissues...
  26. pmc Comparative genomic sequencing identifies novel tissue-specific enhancers and sequence elements for methylation-sensitive factors implicated in Igf2/H19 imprinting
    K Ishihara
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Genome Res 10:664-71. 2000
    ..Our results demonstrate that comparative genomic sequencing is highly efficient in identifying regulatory elements...
  27. ncbi Effect of TSIX disruption on XIST expression in male ES cells
    T Sado
    Division of Human Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Japan
    Cytogenet Genome Res 99:115-8. 2002
    ..Such ectopic expression, however, eventually ceased during prolonged culture. It is likely that surveillance by the X chromosome counting mechanism somehow shuts off the ectopic expression of XIST before inactivation of the X chromosome...
  28. pmc Sequence-specific microscopic visualization of DNA methylation status at satellite repeats in individual cell nuclei and chromosomes
    Yufeng Li
    Division of Epigenomics and Development, Medical Institute of Bioregulation, and Epigenome Network Research Center, Kyushu University, Fukuoka 812 8582, Japan, The Cancer Institute, Tangshan People s Hospital, Hebei 063001, China, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Universite de Strasbourg, F 67404 Illkirch, Cité Universitaire de Strasbourg, France, Department of Epigenetics Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi 409 3898, Japan, Division of Medical Genetics, Saitama Children s Medical Center, Saitama 339 8551, Japan and Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 113 8656, Japan
    Nucleic Acids Res 41:e186. 2013
    ..MeFISH will be suitable for a wide range of applications in epigenetics research and medical diagnosis. ..
  29. ncbi De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, 1111 Yata, Mishima 411 8540, Japan
    Development 131:975-82. 2004
    ..We also demonstrate that delayed upregulation of Xist does not induce X-inactivation, consistent with a crucial developmental window for the chromosomal silencing...
  30. ncbi Bisulfite sequencing and dinucleotide content analysis of 15 imprinted mouse differentially methylated regions (DMRs): paternally methylated DMRs contain less CpGs than maternally methylated DMRs
    H Kobayashi
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Japan
    Cytogenet Genome Res 113:130-7. 2006
    ..Our findings provide a basis for the further characterization of DMRs...
  31. ncbi Role of de novo DNA methyltransferases in initiation of genomic imprinting and X-chromosome inactivation
    M Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems ROIS, Mishima 411 8540, Japan
    Cold Spring Harb Symp Quant Biol 69:125-9. 2004
  32. ncbi Sequence polymorphisms, allelic expression status and chromosome locations of the chicken IGF2 and MPR1 genes
    T Yokomine
    Division of Human Genetics, National Institute of Genetics, Graduate University for Advanced Studies, 1-111 Yata, Mishima 411-8540, Japan
    Cytogenet Cell Genet 93:109-13. 2001
    ..IGF2 and MPR1 were mapped on chicken chromosomes 5 and 3, respectively, by fluorescence in situ hybridization, demonstrating conserved linkage homology between mammals and birds...
  33. ncbi Analysis of small RNA profiles during development
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Japan
    Methods Enzymol 427:155-69. 2007
    ..In this chapter, we describe the methods of small RNA cloning, annotation and classification, and their expression analysis during development...
  34. ncbi Three novel DNMT3B mutations in Japanese patients with ICF syndrome
    Hisao Shirohzu
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Mishima, Japan
    Am J Med Genet 112:31-7. 2002
    ..This is the first missense mutation mapped to the N-terminal half of the protein, suggesting that the region plays an important role in the regulation of the DNMT3B enzyme...
  35. ncbi Evolution of the vertebrate DNMT3 gene family: a possible link between existence of DNMT3L and genomic imprinting
    T Yokomine
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Japan
    Cytogenet Genome Res 113:75-80. 2006
    ..The acquisition of DNMT3L by a common ancestor of eutherians and marsupials might have been closely related to the evolution of imprinting...
  36. ncbi Disruption of mesodermal enhancers for Igf2 in the minute mutant
    Karen Davies
    Laboratory of Developmental Genetics and Imprinting, Developmental Genetics Programme, Babraham Institute, Cambridge CB2 4AT, UK
    Development 129:1657-68. 2002
    ..Our work shows that most known enhancers for Igf2 are located 3' of H19 and establishes an important genetic paradigm for the inheritance of complex regulatory mutations in imprinted gene clusters...
  37. ncbi Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification
    Tatsuya Ohhata
    PRESTO, Japan Science and Technology Agency JST Saitama, 332 0012, Japan
    Development 135:227-35. 2008
    ..These results suggest a crucial role for antisense transcription across the Xist promoter in Xist silencing...
  38. pmc Synergistic function of DNA methyltransferases Dnmt3a and Dnmt3b in the methylation of Oct4 and Nanog
    Jing Yu Li
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, People s Republic of China
    Mol Cell Biol 27:8748-59. 2007
    ..The physical and functional interaction between Dnmt3a and Dnmt3b represents a novel regulatory mechanism to ensure the proper establishment of genomic methylation patterns for gene silencing in development...
  39. ncbi Correlation between Shh expression and DNA methylation status of the limb-specific Shh enhancer region during limb regeneration in amphibians
    Nayuta Yakushiji
    Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama Aoba ku, Sendai 980 8578, Japan
    Dev Biol 312:171-82. 2007
    ..These findings provide the first evidence for a relationship between epigenetic regulation and pattern formation during organ regeneration in vertebrates...
  40. ncbi Establishment and characterization of cultured epithelial cells lacking expression of ZO-1
    Kazuaki Umeda
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Biol Chem 279:44785-94. 2004
    ..Finally, we discussed advantageous aspects of the gene knock-out system with cultured epithelial cells in epithelial cell biology...
  41. pmc DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes
    Satomi Kuramochi-Miyagawa
    Department of Pathology, Medical School, Graduate School of Frontier Biosciences, Research Institute for Microbial Diseases, Osaka University, Yamada oka 2 2 Suita, Osaka 565 0871, Japan
    Genes Dev 22:908-17. 2008
    ..Our data strongly suggest that MILI and MIWI2 play essential roles in establishing de novo DNA methylation of retrotransposons in fetal male germ cells...
  42. ncbi Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses
    Yusuke Sotomaru
    Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243 0034, Japan
    J Biol Chem 277:12474-8. 2002
    ....
  43. ncbi Regulation of DNA methylation activity through Dnmt3L promoter methylation by Dnmt3 enzymes in embryonic development
    Ye Guang Hu
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, People s Republic of China
    Hum Mol Genet 17:2654-64. 2008
    ..Insufficient methylation of the DNMT3L promoter during embryonic development due to deficiency in DNMT3B might be implicated in the pathogenesis of the ICF syndrome...
  44. ncbi [Abnormalities in cloned animals and epigenetics]
    Tomohiro Kawano
    Tanpakushitsu Kakusan Koso 47:1785-7. 2002
  45. ncbi Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients
    Hisato Kobayashi
    Department of Obstetrics, Tohoku University Graduate School of Medicine, 2 1 Seiryo cho, Aoba ku, Sendai 980 8575, Japan
    Hum Mol Genet 16:2542-51. 2007
    ..Our data suggest that sperm from infertile patients, especially those with oligospermia, may carry a higher risk of transmitting incorrect primary imprints to their offspring, highlighting the need for more research into ART...
  46. ncbi Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L
    Zheng Hua Xie
    The State Key Laboratory of Molecular Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
    Hum Mol Genet 15:1375-85. 2006
    ....
  47. ncbi X-inactivation is stably maintained in mouse embryos deficient for histone methyl transferase G9a
    Tatsuya Ohhata
    PRESTO, Japan Science and Technology Agency JST, Kawaguchi, Japan
    Genesis 40:151-6. 2004
    ..These results demonstrate that G9a is not essential for X-inactivation...
  48. ncbi Microarray analysis of promoter methylation in lung cancers
    Masayuki Fukasawa
    Laboratory of Genome Science, Biosignal Genome Resource Center, Department of Molecular and Cellular Biology, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Hum Genet 51:368-74. 2006
    ..This demonstrates that our PMAD method is effective at finding epigenetic changes during cancer...
  49. ncbi Loss of the maternal imprint in Dnmt3Lmat-/- mice leads to a differentiation defect in the extraembryonic tissue
    Takahiro Arima
    Department of Molecular Genetics, Division of Molecular and Cell Therapeutics, Medical Institute of Bioregulation, Kyusyu University, Beppu, Oita 874 0838, Japan
    Dev Biol 297:361-73. 2006
    ..These findings provide evidence that not only is DNA methylation required for the appropriate maternal imprint in the placenta but that the appropriate imprint is absolutely required for vertebrate placentation...
  50. ncbi Stochastic imprinting in the progeny of Dnmt3L-/- females
    Philippe Arnaud
    Institute of Molecular Genetics, CNRS UMR 5535 and University of Montpellier II, 1919 Route de Mende, 34090 Montpellier, France
    Hum Mol Genet 15:589-98. 2006
    ..This observation can provide a basis to understand mechanisms involved in some sporadic cases of imprinting-related diseases and polymorphic imprinting in human...
  51. ncbi The two-domain hypothesis in Beckwith-Wiedemann syndrome: autonomous imprinting of the telomeric domain of the distal chromosome 7 cluster
    Flavia Cerrato
    Dipartimento di Scienze Ambientali, Seconda Universita di Napoli, Via Vivaldi 43, 81100 Caserta, Italy
    Hum Mol Genet 14:503-11. 2005
    ....
  52. pmc CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex
    Yuko Mimori-Kiyosue
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    J Cell Biol 168:141-53. 2005
    ..We propose that CLASPs can mediate interactions between MT plus ends and the cell cortex and act as local rescue factors, possibly through forming a complex with EB1 at MT tips...
  53. pmc Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice
    Takehiro Nitta
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Cell Biol 161:653-60. 2003
    ..e., the size-selective loosening of the BBB) not only provides new insight into the basic molecular physiology of BBB but also opens a new way to deliver potential drugs across the BBB into the central nervous system...
  54. ncbi Genomic organization and promoter analysis of the Dnmt3b gene
    Chisaki Ishida
    Division of Gene Therapy Science, Osaka University School of Medicine, 2 2 Yamada oka, Suita, 565 0870, Osaka, Japan
    Gene 310:151-9. 2003
    ..9 kb upstream of Dnmt3b downregulated this gene specifically in somatic cells but not in ES cells. These findings provide a basis for future detailed studies of the mechanisms controlling Dnmt3b expression...
  55. pmc Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells
    Junichi Ikenouchi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 171:939-45. 2005
    ..These findings indicate the critical function of tricellulin for formation of the epithelial barrier...
  56. ncbi Claudins in Caenorhabditis elegans: their distribution and barrier function in the epithelium
    Akira Asano
    Tsukita Cell Axis Project, ERATO, Japan Science and Technology Corporation, Kyoto Research Park, Shimogyo ku, Japan
    Curr Biol 13:1042-6. 2003
    ..These findings indicated that multiple species of the claudin homologs, which are involved in the barrier function of the epithelium, exist in C. elegans...
  57. ncbi ICF syndrome in a girl with DNA hypomethylation but without detectable DNMT3B mutation
    Takeo Kubota
    Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Kodaira, Japan
    Am J Med Genet A 129:290-3. 2004
    ..A mutation may be present in the gene but undetected, present in other DNA methyltransferases (DNMT) genes or in a DNMT-associated protein gene...
  58. ncbi Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment
    Yuko Mimori-Kiyosue
    KAN Research Institute, Kyoto Research Park, Kyoto 600 8815, Japan
    Genes Cells 11:845-57. 2006
    ..This local stabilization of microtubules is essential for the formation of normal metaphase spindle, completion of anaphase and cytokinesis...
  59. doi Megaintestine in claudin-15-deficient mice
    Atsushi Tamura
    Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka, Japan
    Gastroenterology 134:523-34. 2008
    ..Here, we generated claudin-15-deficient (Cldn15(-/-)) mice to examine the ubiquitously expressed functions of claudin-15...
  60. pmc Radixin deficiency causes deafness associated with progressive degeneration of cochlear stereocilia
    Shin ichiro Kitajiri
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 166:559-70. 2004
    ..These findings indicated the existence of complicate functional redundancy in situ among ERM proteins...
  61. ncbi ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation
    Kazuaki Umeda
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Kyoto 606 8501, Japan
    Cell 126:741-54. 2006
    ..When NZO-1 was forcibly recruited to lateral membranes and dimerized, claudins were dramatically polymerized. These findings indicate that ZO-1 and ZO-2 can independently determine whether and where claudins are polymerized...
  62. ncbi Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential
    Shin ichiro Kitajiri
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, 606 8501, Japan
    J Cell Sci 117:5087-96. 2004
    ....
  63. pmc Tight junctions in Schwann cells of peripheral myelinated axons: a lesson from claudin-19-deficient mice
    Tatsuo Miyamoto
    Department of Cell Biology, Graduate School of Medicine, Kyoto University, Japan
    J Cell Biol 169:527-38. 2005
    ....