M Saijo

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. pmc A patient with Crimean-Congo hemorrhagic fever serologically diagnosed by recombinant nucleoprotein-based antibody detection systems
    Qing Tang
    Second Division of Viral Hemorrhagic Fever, Institute of Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People s Republic of China
    Clin Diagn Lab Immunol 10:489-91. 2003
  2. doi request reprint Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus
    Atsushi Yamanaka
    Department of Internal Medicine, Miyazaki Prefectural Miyazaki Hospital, Miyazaki, Miyazaki, Japan
    PLoS ONE 9:e92777. 2014
  3. pmc Analysis of the humoral immune responses among cynomolgus macaque naturally infected with Reston virus during the 1996 outbreak in the Philippines
    Satoshi Taniguchi
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, 208 0011, Japan
    BMC Vet Res 8:189. 2012
  4. pmc A seroepidemiologic study of Reston ebolavirus in swine in the Philippines
    Yusuke Sayama
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, 208 0011, Japan
    BMC Vet Res 8:82. 2012
  5. doi request reprint Real-time quantitative polymerase chain reaction for virus infection diagnostics
    Masayuki Saijo
    National Institute of Infectious Diseases, Department of Virology 1, 1 23 1 Toyoma, Shinjuku ku, Tokyo 162 8640, Japan 81 3 5285 1111 81 3 5285 2115
    Expert Opin Med Diagn 2:1155-71. 2008
  6. pmc Characteristics of progressive multifocal leukoencephalopathy clarified through internet-assisted laboratory surveillance in Japan
    Kazuo Nakamichi
    Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, 162 8640, Japan
    BMC Neurol 12:121. 2012
  7. ncbi request reprint Inhibitory effect of mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Antiviral Res 66:159-63. 2005
  8. ncbi request reprint Bone marrow transplantation in a child with Wiskott-Aldrich syndrome latently infected with acyclovir-resistant (ACV(r)) herpes simplex virus type 1: emergence of foscarnet-resistant virus originating from the ACV(r) virus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    J Med Virol 68:99-104. 2002
  9. ncbi request reprint Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 59:323-5. 2006
  10. ncbi request reprint Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama 208 0011, Tokyo, Japan
    J Virol Methods 125:181-6. 2005

Collaborators

Detail Information

Publications91

  1. pmc A patient with Crimean-Congo hemorrhagic fever serologically diagnosed by recombinant nucleoprotein-based antibody detection systems
    Qing Tang
    Second Division of Viral Hemorrhagic Fever, Institute of Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People s Republic of China
    Clin Diagn Lab Immunol 10:489-91. 2003
    ..The study indicates that rNP-based CCHF virus antibody detection systems are useful for confirming CCHF virus infections. This case also suggests that intravenous ribavirin therapy may be promising for the treatment of CCHF patients...
  2. doi request reprint Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus
    Atsushi Yamanaka
    Department of Internal Medicine, Miyazaki Prefectural Miyazaki Hospital, Miyazaki, Miyazaki, Japan
    PLoS ONE 9:e92777. 2014
    ..Furthermore, contact surveillance study suggests that the risk of human-to-human infection of the species of Nelson Bay orthoreovirus would seem to be low. ..
  3. pmc Analysis of the humoral immune responses among cynomolgus macaque naturally infected with Reston virus during the 1996 outbreak in the Philippines
    Satoshi Taniguchi
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, 208 0011, Japan
    BMC Vet Res 8:189. 2012
    ....
  4. pmc A seroepidemiologic study of Reston ebolavirus in swine in the Philippines
    Yusuke Sayama
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, 208 0011, Japan
    BMC Vet Res 8:82. 2012
    ..In late 2008, REBOV infections were identified in swine for the first time in the Philippines...
  5. doi request reprint Real-time quantitative polymerase chain reaction for virus infection diagnostics
    Masayuki Saijo
    National Institute of Infectious Diseases, Department of Virology 1, 1 23 1 Toyoma, Shinjuku ku, Tokyo 162 8640, Japan 81 3 5285 1111 81 3 5285 2115
    Expert Opin Med Diagn 2:1155-71. 2008
    ....
  6. pmc Characteristics of progressive multifocal leukoencephalopathy clarified through internet-assisted laboratory surveillance in Japan
    Kazuo Nakamichi
    Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, 162 8640, Japan
    BMC Neurol 12:121. 2012
    ..This study was conducted to elucidate the characteristics of PML in Japan through an internet-assisted laboratory surveillance program...
  7. ncbi request reprint Inhibitory effect of mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Antiviral Res 66:159-63. 2005
    ..Mizoribine inhibited replication of SARS-CoV more strongly than ribavirin. However, neither drug could completely inhibit replication of SARS-CoV even at concentrations up to 100 microg/ml...
  8. ncbi request reprint Bone marrow transplantation in a child with Wiskott-Aldrich syndrome latently infected with acyclovir-resistant (ACV(r)) herpes simplex virus type 1: emergence of foscarnet-resistant virus originating from the ACV(r) virus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    J Med Virol 68:99-104. 2002
    ..This is the first report on the clinical course of a BMT-associated ACV(r) HSV-1 infection that subsequently developed resistance to foscarnet as well...
  9. ncbi request reprint Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 59:323-5. 2006
    ..These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF...
  10. ncbi request reprint Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama 208 0011, Tokyo, Japan
    J Virol Methods 125:181-6. 2005
    ..The SARS-CoV rNP-based IgG ELISA has an advantage over the conventional IgG ELISA in that the antigen can be prepared by laboratory workers without the risk of infection...
  11. pmc Development of recombinant nucleoprotein-based diagnostic systems for Lassa fever
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Clin Vaccine Immunol 14:1182-9. 2007
    ..These LASV rNP-based diagnostics were confirmed to be useful in the diagnosis of LF even in institutes without a high containment laboratory, since the antigens can be prepared without manipulation of the infectious viruses...
  12. pmc LC16m8, a highly attenuated vaccinia virus vaccine lacking expression of the membrane protein B5R, protects monkeys from monkeypox
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 80:5179-88. 2006
    ..These results indicate that LC16m8 prevents lethal monkeypox in monkeys, and they suggest that LC16m8 may induce protective immunity against smallpox...
  13. doi request reprint Virulence and pathophysiology of the Congo Basin and West African strains of monkeypox virus in non-human primates
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases NIID, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Gen Virol 90:2266-71. 2009
    ..Zr-599 affected respiratory, genito-urinary and gastrointestinal tract organs more severely than Liberia. Zr-599 was more virulent than Liberia and one of the factors might be the difference in organ tropism...
  14. ncbi request reprint Antigen-capture enzyme-linked immunosorbent assay for the diagnosis of crimean-congo hemorrhagic fever using a novel monoclonal antibody
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, Japan
    J Med Virol 77:83-8. 2005
    ..However, the method may be useful for the diagnosis of CCHF patients in the acute stage of illness, especially in laboratories not equipped with RT-PCR testing capabilities...
  15. ncbi request reprint Recombinant nucleoprotein-based serological diagnosis of Crimean-Congo hemorrhagic fever virus infections
    Masayuki Saijo
    Department of Virology 1, Special Pathogens Laboratory, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 75:295-9. 2005
    ..It is concluded that this newly developed CCHFV rNP-based IgM-capture ELISA is a useful method for the diagnosis of CCHFV infections...
  16. pmc Genotypic characterization of the DNA polymerase and sensitivity to antiviral compounds of foscarnet-resistant herpes simplex virus type 1 (HSV-1) derived from a foscarnet-sensitive HSV-1 strain
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Antimicrob Agents Chemother 49:606-11. 2005
    ..Seven, 5, and 15 of the 27 isolates were also sensitive to S2242, acyclovir, and penciclovir, respectively. Thus, most of the foscarnet-resistant HSV-1 isolates were sensitive or hypersensitive to cidofovir and ganciclovir...
  17. ncbi request reprint [Crimean-Congo hemorrhagic fever]
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Uirusu 54:223-7. 2004
    ..CCHFV infections are closely associated with the environmental conditions, life styles, religion, occupation, and human economic activities. Based on these data, preventive measures for CCHFV infections are also discussed...
  18. ncbi request reprint Characterization of monoclonal antibodies to Marburg virus nucleoprotein (NP) that can be used for NP-capture enzyme-linked immunosorbent assay
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 76:111-8. 2005
    ..These data suggest that the Marburg virus nucleoprotein-capture ELISA system using the monoclonal antibodies is a promising technique for rapid diagnosis of Marburg hemorrhagic fever...
  19. pmc Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Clin Vaccine Immunol 13:444-51. 2006
  20. ncbi request reprint Characterization and susceptibility to antiviral agents of herpes simplex virus type 1 containing a unique thymidine kinase gene with an amber codon between the first and the second initiation codons
    M Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musahimurayama, Tokyo, Japan
    Arch Virol 153:303-14. 2008
    ....
  21. ncbi request reprint Diagnosis and assessment of monkeypox virus (MPXV) infection by quantitative PCR assay: differentiation of Congo Basin and West African MPXV strains
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 61:140-2. 2008
    ..It was possible to differentiate between these two groups with the LC-qPCR. Thus, the newly developed LC-qPCR is a useful and reliable diagnostic tool for MPXV infection...
  22. ncbi request reprint Importance of C-terminus of herpes simplex virus type 1 thymidine kinase for maintaining thymidine kinase and acyclovir-phosphorylation activities
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 66:388-93. 2002
    ..These mutant TK polypeptides did not phosphorylate ACV. These results indicate that amino acid residues from positions 371-376 in the C-terminal portion of HSV-1 TK protein are essential for keeping TK and ACV-phosphorylation activities...
  23. ncbi request reprint Nucleotide sequence of thymidine kinase gene of sequential acyclovir-resistant herpes simplex virus type 1 isolates recovered from a child with Wiskott-Aldrich syndrome: evidence for reactivation of acyclovir-resistant herpes simplex virus
    M Saijo
    Department of Pediatrics, Asahikawa Medical College, Hokkaido, Japan
    J Med Virol 58:387-93. 1999
    ..These results suggest that the ACV-r HSV-1 might have derived from the ACV-s strain in the patient body and that TK-associated ACV-r HSV-1 can reactivate from latency...
  24. ncbi request reprint Recurrent aciclovir-resistant herpes simplex in a child with Wiskott-Aldrich syndrome
    M Saijo
    Department of Pediatrics, Asahikawa Medical College, 4 5 3 11 Nishikagura, Asahikawa, Hokkaido 078, Japan
    Br J Dermatol 139:311-4. 1998
    ..Diminished sensitivity of HSV-1 TAR and R95 to aciclovir was associated with reduced viral TK activity and loss of aciclovir phosphorylation activity...
  25. ncbi request reprint Genotypic and phenotypic characterization of the thymidine kinase of ACV-resistant HSV-1 derived from an acyclovir-sensitive herpes simplex virus type 1 strain
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Antiviral Res 56:253-62. 2002
    ..Nucleotide sequencing of the TK gene and Western blot analysis of the viral TK polypeptides are considered to be one of the methods for predicting virus sensitivity to ACV and CDV...
  26. pmc Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus
    T Ikegami
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Epidemiol Infect 130:533-9. 2003
    ..The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes...
  27. pmc Enzyme-linked immunosorbent assays for detection of antibodies to Ebola and Marburg viruses using recombinant nucleoproteins
    M Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Clin Microbiol 39:1-7. 2001
    ..The results indicate that ELISA systems prepared with the recombinant NPs of EBO and MBG are valuable tools for the diagnosis of EBO and MBG infections and for seroepidemiological field studies...
  28. pmc Rapid phenotypic characterization method for herpes simplex virus and Varicella-Zoster virus thymidine kinases to screen for acyclovir-resistant viral infection
    T Suzutani
    Department of Microbiology, Asahikawa Medical College, Asahikawa, Japan
    J Clin Microbiol 38:1839-44. 2000
    ..The assay should be a powerful tool in monitoring drug-resistant viruses, especially in cases in which virus isolation is difficult...
  29. pmc Immunofluorescence method for detection of Ebola virus immunoglobulin g, using HeLa cells which express recombinant nucleoprotein
    M Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musahimurayama, Tokyo 208-0011, Japan
    J Clin Microbiol 39:776-8. 2001
    ..This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies...
  30. pmc Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein
    M Niikura
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    J Clin Microbiol 39:3267-71. 2001
    ....
  31. ncbi request reprint cDNA sequence and chromosomal localization of a novel human protein, RBQ-1 (RBBP6), that binds to the retinoblastoma gene product
    Y Sakai
    Biology Division, National Cancer Center Research Institute, Tokyo, Japan
    Genomics 30:98-101. 1995
    ..We have mapped this gene to 16p11.2-p12 using polymerase chain reaction analysis on a human-hamster hybrid cell panel and chromosomal fluorescence in situ hybridization...
  32. ncbi request reprint Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays
    Tang Qing
    Second Division of Viral Hemorrhagic Fever, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Changping, Beijing 102206, PR China
    J Virol Methods 108:111-6. 2003
    ..Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera...
  33. ncbi request reprint Genome structure of Ebola virus subtype Reston: differences among Ebola subtypes. Brief report
    T Ikegami
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Arch Virol 146:2021-7. 2001
    ..The glycoprotein (GP) had several amino acid differences from EBO-R isolated in 1989 and 1992. The variety of GP sequences strongly suggests the independent introduction of EBO-R from unknown natural reservoirs in 1996...
  34. pmc Immunofluorescence technique using HeLa cells expressing recombinant nucleoprotein for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1 AIDS Research Center, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Clin Microbiol 40:372-5. 2002
    ..The novel IF technique using CCHFV rNP-expressing HeLa cells can be used not only for diagnosis of CCHF but also for epidemiological studies on CCHFV infections...
  35. pmc An attenuated LC16m8 smallpox vaccine: analysis of full-genome sequence and induction of immune protection
    Shigeru Morikawa
    Department of Virology, National Institute of Infectious Diseases, Toyama, Tokyo, Japan
    J Virol 79:11873-91. 2005
    ..These results suggest that priming with m8 IMV provides efficient protection despite undetectable levels of immunity against EEV...
  36. pmc Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein
    Tetsuro Ikegami
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Clin Diagn Lab Immunol 10:552-7. 2003
    ..The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques...
  37. ncbi request reprint An improved procedure for rapid determination of viral RNA sequences of avian RNA viruses
    K Sakai
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Arch Virol 152:1763-5. 2007
    ..A system for rapid determination of viral RNA sequences, RDV, was improved for detection of avian RNA virus in allantoic fluids. We detected avian paramyxovirus nucleotide sequences using RDV method ver 2.0...
  38. pmc Differential mutation patterns in thymidine kinase and DNA polymerase genes of herpes simplex virus type 1 clones passaged in the presence of acyclovir or penciclovir
    Tatsuo Suzutani
    Department of Microbiology, Fukushima Medical University, Fukushima, Tokyo, Japan
    Antimicrob Agents Chemother 47:1707-13. 2003
    ..These results clearly point to differential mutation patterns between ACV(r) and PCV(r) HSV-1 clones...
  39. ncbi request reprint Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype Reston by the use of recombinant nucleoprotein-expressing HeLa cells
    Tetsuro Ikegami
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Microbiol Immunol 46:633-8. 2002
    ..These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys...
  40. ncbi request reprint [Serologic studies of Xinjiang hemorrhagic fever in Bachu county, 2001]
    Lei Han
    Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
    Zhonghua Liu Xing Bing Xue Za Zhi 23:179-81. 2002
    ..To investigate the situation of Xinjiang hemorrhagic fever (XHF) in patients who have been diagnosed as XHF by clinical methods and to predict the condition in people who were liable to infection and in the host-animals...
  41. ncbi request reprint Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
    T Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Arch Virol 152:1019-25. 2007
    ..fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis...
  42. ncbi request reprint Pathological and virological analyses of severe acute respiratory syndrome-associated coronavirus infections in experimental animals
    Noriyo Nagata
    National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Adv Exp Med Biol 581:515-8. 2006
  43. pmc Recombinant nucleoprotein-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Clin Microbiol 40:1587-91. 2002
    ..These results suggest that the IgG ELISA system developed with the recombinant CCHFV NP is a valuable tool for diagnosis and epidemiological investigations of CCHFV infections...
  44. ncbi request reprint Characterization of persistent SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    National Institute of Infectious Diseases, Tokyo, Japan
    Adv Exp Med Biol 581:323-6. 2006
  45. ncbi request reprint Modification of endothelial cell functions by Hantaan virus infection: prolonged hyper-permeability induced by TNF-alpha of hantaan virus-infected endothelial cell monolayers
    M Niikura
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Japan
    Arch Virol 149:1279-92. 2004
    ....
  46. ncbi request reprint Emergence and spread of a new clone of M type 1 group A Streptococcus coincident with the increase in invasive diseases in Japan
    K Murono
    Department of Pediatrics, Asahikawa Medical College, Nishikagura, Japan
    Pediatr Infect Dis J 18:254-7. 1999
    ..As is the case in the United States and Europe, M1 serotype is predominant among the isolates from Japanese patients...
  47. pmc Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
    Mina Nakauchi
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Clin Vaccine Immunol 16:1132-8. 2009
    ..The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus...
  48. ncbi request reprint Possible horizontal transmission of crimean-congo hemorrhagic Fever virus from a mother to her child
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 57:55-7. 2004
    ..This case indicates the importance of preventive measures for in-house outbreaks of CCHF...
  49. ncbi request reprint Pseudotyped vesicular stomatitis virus for functional analysis of SARS coronavirus spike protein
    Shuetsu Fukushi
    National Institute of Infectious Diseases, Tokyo, Japan
    Adv Exp Med Biol 581:293-6. 2006
  50. pmc Persisting humoral antiviral immunity within the Japanese population after the discontinuation in 1976 of routine smallpox vaccinations
    Shuji Hatakeyama
    Department of Infectious Diseases, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo, Japan
    Clin Diagn Lab Immunol 12:520-4. 2005
    ..This long-lasting immunity may provide some protective benefits in the case of reemergence of smallpox, and the disease may not spread as widely and fatally as generally expected...
  51. ncbi request reprint Current knowledge on lower virulence of Reston Ebola virus (in French: Connaissances actuelles sur la moindre virulence du virus Ebola Reston)
    Shigeru Morikawa
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Comp Immunol Microbiol Infect Dis 30:391-8. 2007
    ..Recent studies indicated several factors involved in different virulence between African EBOV and REBOV. This article reviews the history, epidemiology, and virulence of REBOV...
  52. doi request reprint Long-term infection of adult mice with murine polyomavirus following stereotaxic inoculation into the brain
    Kazuo Nakamichi
    Department of Virology 1, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku, Tokyo 162 8640, Japan
    Microbiol Immunol 54:475-82. 2010
    ..These results indicate that MPyV establishes an asymptomatic long-term infection in the mouse brain...
  53. pmc Mouse-passaged severe acute respiratory syndrome-associated coronavirus leads to lethal pulmonary edema and diffuse alveolar damage in adult but not young mice
    Noriyo Nagata
    Department of Pathology, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    Am J Pathol 172:1625-37. 2008
    ..The experimental model described here may be useful for elucidating the pathophysiology of SARS and for evaluating therapies to treat SARS-CoV infection...
  54. pmc Amino acid substitutions in the s2 region enhance severe acute respiratory syndrome coronavirus infectivity in rat angiotensin-converting enzyme 2-expressing cells
    Shuetsu Fukushi
    Special Pathogens Laboratory, Department of Virology I, Center for Pathogens Genomics, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 81:10831-4. 2007
    ..Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region...
  55. ncbi request reprint Recent progress in molecular biology of Crimean-Congo hemorrhagic fever
    Shigeru Morikawa
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Comp Immunol Microbiol Infect Dis 30:375-89. 2007
    ..These analyses indicate that M-RNA segment reassortment events occur more frequently than those in S- and L-RNA segments...
  56. doi request reprint Celastrol suppresses morphological and transcriptional responses in microglial cells upon stimulation with double-stranded RNA
    Kazuo Nakamichi
    Laboratory of Neurovirology, Department of Virology 1, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    Int J Neurosci 120:252-7. 2010
    ..These data demonstrate that celastrol inhibits morphological and transcriptional responses during microglial activation...
  57. pmc Pathology and virus dispersion in cynomolgus monkeys experimentally infected with severe acute respiratory syndrome coronavirus via different inoculation routes
    Noriyo Nagata
    Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan
    Int J Exp Pathol 88:403-14. 2007
    ..t. inoculation. The virus appears to spread into and perhaps via the intestinal and lymphatic systems. It has been suggested previously that viraemia may cause intestinal infections in SARS patients...
  58. doi request reprint Clinical and immunological response to attenuated tissue-cultured smallpox vaccine LC16m8
    Tomoya Saito
    Bio preparedness Research Laboratory, Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, Tokyo, Japan
    JAMA 301:1025-33. 2009
    ..It is a good candidate for use as part of a prevention plan for bioterrorism...
  59. pmc Rapid genome sequencing of RNA viruses
    Tetsuya Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama City, Tokyo, Japan
    Emerg Infect Dis 13:322-4. 2007
    ..This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses...
  60. ncbi request reprint Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    FEMS Immunol Med Microbiol 46:236-43. 2006
    ..This is the first study to analyze SARS-CoV-induced cell growth inhibition...
  61. ncbi request reprint Evaluation of a novel vesicular stomatitis virus pseudotype-based assay for detection of neutralizing antibody responses to SARS-CoV
    Shuetsu Fukushi
    Department of Virology I, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 78:1509-12. 2006
    ..The newly developed neutralization assay was more sensitive to low antibody titers in serum samples. Thus, the VSV-SARS-St19 is a useful tool for detecting Nabs against SARS-CoV...
  62. ncbi request reprint Regulation of p90RSK phosphorylation by SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    FEBS Lett 580:1417-24. 2006
    ..These results indicated that phosphorylation status of p90RSK by SARS-CoV infection is different from that by stimulation of EGF. This is the first detailed report regarding regulation of p90RSK phosphorylation by virus infection...
  63. ncbi request reprint Phosphorylation of p38 MAPK and its downstream targets in SARS coronavirus-infected cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    Biochem Biophys Res Commun 319:1228-34. 2004
    ..The p38 MAPK inhibitor, SB203580, inhibited effectively phosphorylation of HSP-27, CREB, and eIF4E in SARS-CoV-infected cells. However, viral protein synthesis was not affected by treatment of SB203580...
  64. ncbi request reprint Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    FEBS Lett 577:187-92. 2004
    ..As STAT3 acts as an activator of transcription in the nucleus, these results suggest that STAT3 lacks its activity on transcription in SARS-CoV-infected Vero E6 cells...
  65. ncbi request reprint Mechanisms of establishment of persistent SARS-CoV-infected cells
    Tetsuya Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Biochem Biophys Res Commun 347:261-5. 2006
    ..These results indicated that at least four proteins, Akt, JNK, Bcl-2 and Bcl-xL, are necessary for survival of persistently SARS-CoV-infected cells...
  66. ncbi request reprint Diagnosis of human respiratory syncytial virus infection using reverse transcription loop-mediated isothermal amplification
    Kazuya Shirato
    Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Virol Methods 139:78-84. 2007
    ..These results suggest that the RT-LAMP is more sensitive than other methods used to detect RSV. The RT-LAMP assay developed in this study may be useful for diagnostic and epidemiological studies of RSV infection...
  67. ncbi request reprint Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein
    Shuetsu Fukushi
    Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Gen Virol 86:2269-74. 2005
    ..VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection...
  68. ncbi request reprint JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Biochim Biophys Acta 1741:4-10. 2005
    ..These results indicated that two signaling pathways of JNK and PI3K/Akt were important for the establishment of persistence in Vero E6 cells...
  69. pmc Participation of both host and virus factors in induction of severe acute respiratory syndrome (SARS) in F344 rats infected with SARS coronavirus
    Noriyo Nagata
    Department of Pathology, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 81:1848-57. 2007
    ..These results strongly indicate that host and virus factors such as advanced age and virus adaptation are critical for the development of SARS in rats...
  70. ncbi request reprint Genetic diversity of the M RNA segment among Crimean-Congo hemorrhagic fever virus isolates in China
    Shigeru Morikawa
    Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Virology 296:159-64. 2002
    ..Since all the viruses were isolated from 1966 to 1988 within a restricted area in the Xinjiang Autonomous Region in western China, the results indicate that a multisource virus population is endemic in this region...
  71. ncbi request reprint Chimeric recombinant hepatitis E virus-like particles as an oral vaccine vehicle presenting foreign epitopes
    Masahiro Niikura
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Virology 293:273-80. 2002
    ..These results suggest the potential of HEV-VLP as a mucosal vaccine carrier vehicle for the presentation of antigenic epitopes through oral administration...
  72. ncbi request reprint Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Virology 327:169-74. 2004
    ..PKCzeta, which is downstream of the PI3K pathway, was also phosphorylated in virus-infected cells. These results suggested that weak activation of Akt cannot prevent apoptosis induced by SARS-CoV infection in Vero E6 cells...
  73. ncbi request reprint Synthesis and biological evaluation of nucleoside analogues having 6-chloropurine as anti-SARS-CoV agents
    Masahiro Ikejiri
    Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314 1 Shido, Sanuki, Kagawa 769 2193, Japan
    Bioorg Med Chem Lett 17:2470-3. 2007
    ..Moreover, we observed several SAR trends such as the antiviral effects of the 6-chloropurine moiety, unprotected 5'-hydroxyl group and benzoylated 5'-hydroxyl group...
  74. doi request reprint Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)
    Shumpei Watanabe
    Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Clin Virol 43:56-9. 2008
    ..However, some species of viruses, including the Yokose virus (YOKV), a flavivirus, could not be detected using the conventional procedures...
  75. pmc Analysis of linear B-cell epitopes of the nucleoprotein of ebola virus that distinguish ebola virus subtypes
    Masahiro Niikura
    Department of Virology, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Clin Diagn Lab Immunol 10:83-7. 2003
    ..These MAbs showing different subtype specificities might be useful reagents for developing an immunological system to identify Ebola virus subtypes...
  76. ncbi request reprint Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996
    Tetsuro Ikegami
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Exp Anim 51:447-55. 2002
    ..These results indicate that EBO-R infection is characterized by systemic coagulopathy and an increase in blood-derived macrophages/monocytes in accordance with the EBO-R propagation in macrophages/monocytes...
  77. doi request reprint Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections
    Itoe Iizuka
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 81:1102-8. 2009
    ..The LAMP assays made it possible to discriminate between Congo Basin and West African MPXV. The LAMP developed in this study is useful not only for diagnosis of but also for the assessment of MPXV infections...
  78. doi request reprint Co-infection of respiratory bacterium with severe acute respiratory syndrome coronavirus induces an exacerbated pneumonia in mice
    Yasushi Ami
    National Institute of Infectious Diseases, Murayama Branch, Musashi Murayama, Tokyo, Japan
    Microbiol Immunol 52:118-27. 2008
    ....
  79. ncbi request reprint [Diagnostic tests: Sabia virus]
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases
    Nihon Rinsho 63:393-6. 2005
  80. ncbi request reprint Molecular cloning of a human protein that binds to the retinoblastoma protein and chromosomal mapping
    M Saijo
    Biology Division, National Cancer Center Research Institute, Tokyo, Japan
    Genomics 27:511-9. 1995
    ..We have mapped the gene to 1q32 using polymerase chain reaction analysis on a human-hamster hybrid cell panel and chromosomal fluorescence in situ hybridization...
  81. ncbi request reprint [A patient with Streptococcus intermedius brain abscess treated with high dose penicillin G--susceptibility of the isolate to penicillin G and the concentration of penicillin G in cerebrospinal fluid]
    M Saijo
    Department of Pediatrics, Asahikawa Medical College
    Kansenshogaku Zasshi 72:414-7. 1998
    ..S. intermedius must be considered as one of the causative agents for brain abscess. High dose penicillin G therapy is one choice of treatment for brain abscess due to penicillin-susceptible streptococci...
  82. doi request reprint Progressive multifocal leukoencephalopathy developed in incomplete Heerfordt syndrome, a rare manifestation of sarcoidosis, without steroid therapy responding to cidofovir
    Takuya Yagi
    Department of Neurology, Keio University School of Medicine, Tokyo, Japan
    Clin Neurol Neurosurg 112:153-6. 2010
    ....
  83. ncbi request reprint Lymphocytic choriomeningitis infection undetected by dirty-bedding sentinel monitoring and revealed after embryo transfer of an inbred strain derived from wild mice
    Fumio Ike
    RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
    Comp Med 57:272-81. 2007
    ..111..
  84. ncbi request reprint [Crimean-Congo hemorrhagic fever]
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases
    Nihon Rinsho 65:30-4. 2007
  85. doi request reprint Novel reovirus isolation from an Ostrich (Struthio camelus) in Japan
    Kouji Sakai
    Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    Vet Microbiol 134:227-32. 2009
    ..To our knowledge, this is the first genetic report of an orthoreovirus isolated from an ostrich...
  86. ncbi request reprint The intracellular association of the nucleocapsid protein (NP) of hantaan virus (HTNV) with small ubiquitin-like modifier-1 (SUMO-1) conjugating enzyme 9 (Ubc9)
    Akihiko Maeda
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Virology 305:288-97. 2003
    ..These results indicate that the assembly of the HTNV-NP is regulated by the interaction between the NP and Ubc9. This is the first report to demonstrate the interaction of Ubc9 with a structural protein of negative-strand RNA viruses...
  87. ncbi request reprint [Clinical aspects of viral hemorrhagic fever]
    Masayuki Saijo
    Department of Virology I, National Institute of Infectious Diseases
    Nihon Rinsho 63:2161-6. 2005
    ..Therefore, preparedness for possible future outbreaks of VHF is necessary in areas without VHF outbreaks...
  88. ncbi request reprint Rapid determination of viral RNA sequences in mosquitoes collected in the field
    Yuki Kihara
    Faculty of Pharmaceutical Sciences, Fukuoka University, 8 19 1 Nanakuma, Jonan ku, Fukuoka 814 0180, Japan
    J Virol Methods 146:372-4. 2007
    ..Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses...
  89. ncbi request reprint [Anti-vaccinia monoclonal antibodies cross-reactive with monkey pox virus and their application]
    Noritoshi Kitamoto
    School of Human Science and Environment, University of Hyogo
    Kansenshogaku Zasshi 82:224-5. 2008
  90. ncbi request reprint [Lassa fever]
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases
    Nihon Rinsho 65:40-3. 2007