Takashi Sado

Summary

Affiliation: National Institute of Genetics
Country: Japan

Publications

  1. ncbi request reprint Regulation of imprinted X-chromosome inactivation in mice by Tsix
    T Sado
    Division of Human Genetics, National Institute of Genetics, Yata, Mishima, Japan
    Development 128:1275-86. 2001
  2. ncbi request reprint [Molecular mechanisms of X chromosome inactivation]
    Takashi Sado
    Tanpakushitsu Kakusan Koso 51:2471-7. 2006
  3. ncbi request reprint Tsix defective in splicing is competent to establish Xist silencing
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, 411 8540, Japan
    Development 133:4925-31. 2006
  4. ncbi request reprint Tsix silences Xist through modification of chromatin structure
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, Japan
    Dev Cell 9:159-65. 2005
  5. ncbi request reprint De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, 1111 Yata, Mishima 411 8540, Japan
    Development 131:975-82. 2004
  6. ncbi request reprint Effect of TSIX disruption on XIST expression in male ES cells
    T Sado
    Division of Human Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Japan
    Cytogenet Genome Res 99:115-8. 2002
  7. doi request reprint De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:768-74. 2008
  8. pmc Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus
    Toshiaki Watanabe
    Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Science 332:848-52. 2011
  9. doi request reprint A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse
    Yuko Hoki
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima 411 8540, Japan
    Development 136:139-46. 2009
  10. doi request reprint De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:spc one. 2008

Collaborators

Detail Information

Publications20

  1. ncbi request reprint Regulation of imprinted X-chromosome inactivation in mice by Tsix
    T Sado
    Division of Human Genetics, National Institute of Genetics, Yata, Mishima, Japan
    Development 128:1275-86. 2001
    ..These results provide genetic evidence that Tsix plays a crucial role in maintaining Xist silencing in cis and in regulation of imprinted X-inactivation in the extra-embryonic tissues...
  2. ncbi request reprint [Molecular mechanisms of X chromosome inactivation]
    Takashi Sado
    Tanpakushitsu Kakusan Koso 51:2471-7. 2006
  3. ncbi request reprint Tsix defective in splicing is competent to establish Xist silencing
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, 411 8540, Japan
    Development 133:4925-31. 2006
    ..Moreover, the repressive chromatin configuration was properly established at the Xist locus. These unexpected results indicate that the splicing products are dispensable for Tsix-mediated Xist silencing...
  4. ncbi request reprint Tsix silences Xist through modification of chromatin structure
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, Japan
    Dev Cell 9:159-65. 2005
    ..Moreover, we show that disruption of Tsix impairs establishment of repressive epigenetic modifications and chromatin structure at the Xist locus. We propose that Tsix silences Xist through modification of the chromatin structure...
  5. ncbi request reprint De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, 1111 Yata, Mishima 411 8540, Japan
    Development 131:975-82. 2004
    ..We also demonstrate that delayed upregulation of Xist does not induce X-inactivation, consistent with a crucial developmental window for the chromosomal silencing...
  6. ncbi request reprint Effect of TSIX disruption on XIST expression in male ES cells
    T Sado
    Division of Human Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Japan
    Cytogenet Genome Res 99:115-8. 2002
    ..Such ectopic expression, however, eventually ceased during prolonged culture. It is likely that surveillance by the X chromosome counting mechanism somehow shuts off the ectopic expression of XIST before inactivation of the X chromosome...
  7. doi request reprint De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:768-74. 2008
    ..This underscores the difference between imprinted XCI and autosomal imprinting...
  8. pmc Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus
    Toshiaki Watanabe
    Division of Human Genetics and Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Science 332:848-52. 2011
    ..A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1...
  9. doi request reprint A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse
    Yuko Hoki
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima 411 8540, Japan
    Development 136:139-46. 2009
    ....
  10. doi request reprint De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes
    Hatsune Chiba
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Japan
    Genesis 46:spc one. 2008
    ..The study by Chiba et al. in this issue suggests that de novo DNA methyltransferases are dispensable for setting the imprint on the maternally-derived X chromsome in growing oocytes. See Chiba et al. in this issue...
  11. ncbi request reprint Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting
    Masahiro Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 429:900-3. 2004
    ..These results indicate that both Dnmt3a and Dnmt3L are required for methylation of most imprinted loci in germ cells, but also suggest the involvement of other factors...
  12. ncbi request reprint Imprinted X inactivation and reprogramming in the preimplantation mouse embryo
    Takashi Sado
    Division of Human Genetics, National Institute of Genetics, Research Organization of Information Systems, Mishima, Japan
    Hum Mol Genet 14:R59-64. 2005
    ..Neither the underlying reason nor the full extent of these early lineage specific epigenetic changes is known, but they may be correlated with more genome-wide reprogramming events essential for normal development...
  13. ncbi request reprint Large-scale identification and mapping of nuclear matrix-attachment regions in the distal imprinted domain of mouse chromosome 7
    Wahyu Purbowasito
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    DNA Res 11:391-407. 2004
    ..This study presents the first large-scale mapping of MARs in an imprinted domain and provides a platform for understanding the roles of MARs in imprinting...
  14. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011
    ..Our results indicate a conserved role for MITOPLD/Zuc in the piRNA pathway and link mitochondrial membrane metabolism/signaling to small RNA biogenesis...
  15. pmc The CENP-O complex requirement varies among different cell types
    Naoko Kagawa
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka, 411 8540, Japan
    Chromosome Res 22:293-303. 2014
    ..Thus, although both DT40 and ES cells with CENP-U deficiency have similar mitotic defects, cellular responses to mitotic defects vary among different cell types. ..
  16. ncbi request reprint Role of de novo DNA methyltransferases in initiation of genomic imprinting and X-chromosome inactivation
    M Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems ROIS, Mishima 411 8540, Japan
    Cold Spring Harb Symp Quant Biol 69:125-9. 2004
  17. ncbi request reprint Three novel DNMT3B mutations in Japanese patients with ICF syndrome
    Hisao Shirohzu
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Mishima, Japan
    Am J Med Genet 112:31-7. 2002
    ..This is the first missense mutation mapped to the N-terminal half of the protein, suggesting that the region plays an important role in the regulation of the DNMT3B enzyme...
  18. ncbi request reprint [X chromosome inactivation and reactivation during mouse development]
    Yuko Amakawa
    Tanpakushitsu Kakusan Koso 53:830-5. 2008
  19. ncbi request reprint X-inactivation is stably maintained in mouse embryos deficient for histone methyl transferase G9a
    Tatsuya Ohhata
    PRESTO, Japan Science and Technology Agency JST, Kawaguchi, Japan
    Genesis 40:151-6. 2004
    ..These results demonstrate that G9a is not essential for X-inactivation...
  20. ncbi request reprint Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification
    Tatsuya Ohhata
    PRESTO, Japan Science and Technology Agency JST Saitama, 332 0012, Japan
    Development 135:227-35. 2008
    ..These results suggest a crucial role for antisense transcription across the Xist promoter in Xist silencing...