Makoto Ohnishi

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. pmc Identification of TEM-135 beta-lactamase in penicillinase-producing Neisseria gonorrhoeae strains in Japan
    Makoto Ohnishi
    Department of Bacteriology I, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 54:3021-3. 2010
  2. pmc Spread of a chromosomal cefixime-resistant penA gene among different Neisseria gonorrhoeae lineages
    Makoto Ohnishi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 54:1060-7. 2010
  3. pmc Ceftriaxone-Resistant Neisseria gonorrhoeae, Japan
    Makoto Ohnishi
    National Institute of Infectious Diseases, Tokyo, Japan M Ohnishi, S Nakayama, H Watanabe Mitsubishi Chemical Medience Corporation, Tokyo T Saika Hoshina Clinic, Kyoto, Japan S Hoshina and Kyoto Prefectural University of Medicine, Kyoto K Iwasaku, J Kitawaki
    Emerg Infect Dis 17:148-9. 2011
  4. pmc Transcription of the ehx enterohemolysin gene is positively regulated by GrlA, a global regulator encoded within the locus of enterocyte effacement in enterohemorrhagic Escherichia coli
    Takehito Saitoh
    Department of Bacteriology, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Bacteriol 190:4822-30. 2008
  5. ncbi request reprint Characterization of Shigella sonnei isolates from travel-associated cases in Japan
    Hidemasa Izumiya
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
    J Med Microbiol 58:1486-91. 2009
  6. pmc Antimicrobial resistance and molecular typing of Neisseria gonorrhoeae isolates in Kyoto and Osaka, Japan, 2010 to 2012: intensified surveillance after identification of the first strain (H041) with high-level ceftriaxone resistance
    Ken Shimuta
    National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 57:5225-32. 2013
  7. doi request reprint Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae
    Masatomo Morita
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Microbiol Immunol 57:334-9. 2013
  8. pmc Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand
    Shu ichi Nakayama
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 56:916-20. 2012
  9. pmc Coordinate control of the locus of enterocyte effacement and enterohemolysin genes by multiple common virulence regulators in enterohemorrhagic Escherichia coli
    Sunao Iyoda
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Infect Immun 79:4628-37. 2011
  10. pmc Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone
    Makoto Ohnishi
    National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 55:3538-45. 2011

Detail Information

Publications41

  1. pmc Identification of TEM-135 beta-lactamase in penicillinase-producing Neisseria gonorrhoeae strains in Japan
    Makoto Ohnishi
    Department of Bacteriology I, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 54:3021-3. 2010
    ....
  2. pmc Spread of a chromosomal cefixime-resistant penA gene among different Neisseria gonorrhoeae lineages
    Makoto Ohnishi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 54:1060-7. 2010
    ..Our results provide evidence that the capacity for natural transformation in N. gonorrhoeae plays a role in the spread of chromosomal antibiotic resistance genes and the generation of diversity in such genes...
  3. pmc Ceftriaxone-Resistant Neisseria gonorrhoeae, Japan
    Makoto Ohnishi
    National Institute of Infectious Diseases, Tokyo, Japan M Ohnishi, S Nakayama, H Watanabe Mitsubishi Chemical Medience Corporation, Tokyo T Saika Hoshina Clinic, Kyoto, Japan S Hoshina and Kyoto Prefectural University of Medicine, Kyoto K Iwasaku, J Kitawaki
    Emerg Infect Dis 17:148-9. 2011
    ..Best practice treatment is limited to injectable extended-spectrum cephalosporins, such as ceftriaxone and spectinomycin. The emergence of ceftriaxone-resistant N. gonorrhoeae threatens effective disease control...
  4. pmc Transcription of the ehx enterohemolysin gene is positively regulated by GrlA, a global regulator encoded within the locus of enterocyte effacement in enterohemorrhagic Escherichia coli
    Takehito Saitoh
    Department of Bacteriology, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Bacteriol 190:4822-30. 2008
    ..These results together suggest that GrlA acts as a positive regulator for the ehx transcription in EHEC...
  5. ncbi request reprint Characterization of Shigella sonnei isolates from travel-associated cases in Japan
    Hidemasa Izumiya
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
    J Med Microbiol 58:1486-91. 2009
    ..Use of a combination of methods was found to be effective for epidemiological investigation in the case of S. sonnei infection...
  6. pmc Antimicrobial resistance and molecular typing of Neisseria gonorrhoeae isolates in Kyoto and Osaka, Japan, 2010 to 2012: intensified surveillance after identification of the first strain (H041) with high-level ceftriaxone resistance
    Ken Shimuta
    National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 57:5225-32. 2013
    ..gonorrhoeae strain H041, suggesting that H041 might have caused only a sporadic case and has not spread further. ..
  7. doi request reprint Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae
    Masatomo Morita
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Microbiol Immunol 57:334-9. 2013
    ..47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS-related genes occurs among V. cholerae in natural ecosystems...
  8. pmc Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand
    Shu ichi Nakayama
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 56:916-20. 2012
    ..gonorrhoeae in the future. This study identified a bla(TEM) variant (bla(TEM-135)) that is a possible intermediate precursor for an ESBL, which warrants international awareness...
  9. pmc Coordinate control of the locus of enterocyte effacement and enterohemolysin genes by multiple common virulence regulators in enterohemorrhagic Escherichia coli
    Sunao Iyoda
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Infect Immun 79:4628-37. 2011
    ..Together, these results indicate that transcription of ehx is positively regulated by Ler, GrlA, and LrhA, which all act as positive regulators for LEE expression...
  10. pmc Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone
    Makoto Ohnishi
    National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 55:3538-45. 2011
    ..Ultimately, the development of new drugs for efficacious gonorrhea treatment is necessary...
  11. doi request reprint Emergence and genetic diversity of El Tor Vibrio cholerae O1 that possess classical biotype ctxB among travel-associated cases of cholera in Japan
    Masatomo Morita
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 59:708-12. 2010
    ..El Tor variant strains are widespread in Asian countries and show significant genetic diversity, indicating that their spread is a result of multiclonal expansion rather than spread from a single clone...
  12. pmc The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlA
    Ken Shimuta
    Department of Bacteriology I, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    BMC Microbiol 9:261. 2009
    ..There have been few studies of the virulence factors of this bacterium. The only S. marcescens hemolytic and cytotoxic factor reported, thus far, is the hemolysin ShlA...
  13. doi request reprint Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus
    Hidemasa Izumiya
    Department of Bacteriology I, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, Japan
    Mol Cell Probes 25:174-6. 2011
    ..It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness...
  14. pmc Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae
    Shouji Yamamoto
    Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    J Bacteriol 193:1953-65. 2011
    ..Taking these results together, we propose that in the presence of (GlcNAc)₂, TfoR sRNA is expressed to activate the translation of tfoX(VC), which leads to the induction of natural competence...
  15. doi request reprint New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111
    Hidemasa Izumiya
    Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Microbiol Immunol 54:569-77. 2010
    ..Because EHEC strains of three major serogroups can be rapidly analyzed on a single platform with our expanded MLVA system, this system could be widely used in molecular epidemiological studies of EHEC infections...
  16. pmc EspO1-2 regulates EspM2-mediated RhoA activity to stabilize formation of focal adhesions in enterohemorrhagic Escherichia coli-infected host cells
    Tomoko Morita-Ishihara
    Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    PLoS ONE 8:e55960. 2013
    ..These results indicated that EspO1-2 interacted with EspM2 to regulate EspM2-mediated RhoA activity and stabilize FA formation during EHEC infection...
  17. pmc RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion system
    Jiro Mitobe
    Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    EMBO Rep 12:911-6. 2011
    ..Thus, we propose that RodZ is a new membrane-bound RNA-binding protein that provides a scaffold for post-transcriptional regulation...
  18. doi request reprint Molecular and serological investigation of Leptospira and leptospirosis in dogs in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 62:630-6. 2013
    ..This study suggests that the virulence of members of serogroup Hebdomadis in dogs may be associated with the genotypes in this serogroup...
  19. ncbi request reprint [Gonococcal infection]
    Ken Shimuta
    Department of Bacteriology, National Institute of Infectious Diseases
    Nihon Rinsho 65:423-7. 2007
  20. doi request reprint Regulation of natural competence by the orphan two-component system sensor kinase ChiS involves a non-canonical transmembrane regulator in Vibrio cholerae
    Shouji Yamamoto
    Department of Bacteriology I, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo, 162 8640, Japan
    Mol Microbiol 91:326-47. 2014
    ..The results presented here suggest that ChiS operates a chitin-induced non-canonical signal transduction cascade through TfoS, leading to transcriptional activation of tfoR. ..
  21. doi request reprint Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses
    Ryoma Nakao
    Department of Bacteriology I, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan Electronic address
    Microbes Infect 16:6-16. 2014
    ..These data suggest that gingipain-laden OMVs may contribute to tissue destruction in periodontal diseases by serving as a vehicle for the antigens and active proteases. ..
  22. pmc A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan
    J Clin Microbiol 50:2072-4. 2012
    ..in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR...
  23. pmc Interferon-γ-producing immature myeloid cells confer protection against severe invasive group A Streptococcus infections
    Takayuki Matsumura
    Department of Immunology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Nat Commun 3:678. 2012
    ..Our results indicate that interferon-γ-producing immature myeloid cells have a protective role during the early stage of severe invasive group A Streptococcus infections...
  24. pmc Distribution of monoclonal antibody subgroups and sequence-based types among Legionella pneumophila serogroup 1 isolates derived from cooling tower water, bathwater, and soil in Japan
    Junko Amemura-Maekawa
    Department of Bacteriology I, National Institute of Infectious Diseases, Sinjuku ku, Tokyo, Japan
    Appl Environ Microbiol 78:4263-70. 2012
    ..ST1 accounted for 74% of the CT isolates. The only common STs between (i) BW and CT, (ii) BW and SO, and (iii) CT and SO were ST1, ST129, and ST48, respectively, suggesting that each environment constitutes an independent habitat...
  25. pmc Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens
    Tsuyoshi Sekizuka
    Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 6:e25334. 2011
    ..The complete sequence of pNDM-1_Dok01 suggests that the bla(NDM-1) gene was acquired by a novel composite transposon on an extensively disseminated IncA/C plasmid and transferred to the E. coli ST38 isolate...
  26. pmc Outer membrane vesicles of Porphyromonas gingivalis elicit a mucosal immune response
    Ryoma Nakao
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 6:e26163. 2011
    ..In conclusion, the OMVs of P. gingivalis have an important role in mucosal immunogenicity as well as in antigenicity. We propose that P. gingivalis OMV is an intriguing immunogen for development of a periodontal disease vaccine...
  27. ncbi request reprint Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi A
    Masatomo Morita
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
    Microbiol Immunol 50:707-11. 2006
    ..This assay allows rapid screening of S. enterica serovar Typhi and Paratyphi A with reduced susceptibility to ciprofloxacin...
  28. ncbi request reprint Characteristics of Streptococcus suis isolated from patients in Japan
    Bin Chang
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Jpn J Infect Dis 59:397-9. 2006
    ..The remaining isolate was ST28 and its PFGE pattern was distinct from those of the others...
  29. pmc The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny
    Yoshihiko Sakaguchi
    Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Proc Natl Acad Sci U S A 102:17472-7. 2005
    ....
  30. ncbi request reprint Multilocus sequence typing and DNA similarity analysis implicates that a Borrelia valaisiana-related sp. isolated in Japan is distinguishable from European B. valaisiana
    Hiroki Kawabata
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Vet Med Sci 75:1201-7. 2013
    ..valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of B. yangtze(=DSM 24625, JCM 17189)...
  31. doi request reprint Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor
    Masatomo Morita
    National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo, 162 8640, Japan
    Microbiol Immunol 52:314-7. 2008
    ..cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1...
  32. doi request reprint Diagnosis of acute leptospirosis
    Takao Toyokawa
    Field Epidemiology Training Program Japan, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
    Expert Rev Anti Infect Ther 9:111-21. 2011
    ..will accelerate our understanding of Leptospira pathogenesis and provide insights into the development of more efficient and accurate diagnostic tests for acute-phase leptospirosis...
  33. pmc Whole-genome analysis of Salmonella enterica serovar Typhimurium T000240 reveals the acquisition of a genomic island involved in multidrug resistance via IS1 derivatives on the chromosome
    Hidemasa Izumiya
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 55:623-30. 2011
    ..enterica serovar Typhimurium. It is expected that future whole-genome studies will aid in the characterization of the horizontal gene transfer events for the emerging S. enterica serovar Typhimurium strains...
  34. ncbi request reprint A novel approach for purification and selective capture of membrane vesicles of the periodontopathic bacterium, Porphyromonas gingivalis: membrane vesicles bind to magnetic beads coated with epoxy groups in a noncovalent, species-specific manner
    Ryoma Nakao
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 9:e95137. 2014
    ..gingivalis MVs could be selectively eliminated from the mixtures. We suggest that this novel approach enables efficient purification and selective elimination of P. gingivalis MVs...
  35. pmc Meningococcal PilV potentiates Neisseria meningitidis type IV pilus-mediated internalization into human endothelial and epithelial cells
    Hideyuki Takahashi
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
    Infect Immun 80:4154-66. 2012
    ..meningitidis internalization into human cells...
  36. doi request reprint A novel relapsing fever Borrelia sp. infects the salivary glands of the molted hard tick, Amblyomma geoemydae
    Ai Takano
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
    Ticks Tick Borne Dis 3:259-61. 2012
    ..occurred in the tick midgut and the salivary glands, although Borrelia sp. tAG was only detected in the tick midgut. The difference of the borrelial niche in molted ticks might be associated with borrelial characterization...
  37. pmc Multilocus sequence typing implicates rodents as the main reservoir host of human-pathogenic Borrelia garinii in Japan
    Ai Takano
    Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Clin Microbiol 49:2035-9. 2011
    ..Fifty-nine isolates were divided into two phylogenetic groups, and an association was detected between clinical and rodent isolates, suggesting that, in Japan, human-pathogenic B. garinii comes from rodents via ticks...
  38. pmc Competence-dependent endogenous DNA rearrangement and uptake of extracellular DNA give a natural variant of Streptococcus mutans without biofilm formation
    Naoki Narisawa
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Bacteriol 193:5147-54. 2011
    ..Our findings may help in understanding how S. mutans can adapt to the oral environment and may explain the evolution of S. mutans...
  39. ncbi request reprint Serodiagnosis using microagglutination assay during the food-poisoning outbreak in Japan caused by consumption of raw beef contaminated with enterohemorrhagic Escherichia coli O111 and O157
    Junko Isobe
    Department of Bacteriology, Toyama Institute of Health, Imizu, Toyama, Japan
    J Clin Microbiol 52:1112-8. 2014
    ..Measuring antibodies to E. coli O antigen is helpful especially in cases with multiple EHEC infections, even with a non-O157 serotype. ..
  40. pmc Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes
    Takahiro Murata
    Department of Bacteriology, Shinshu University School of Medicine, 3 1 1 Asahi, Matsumoto, Nagano 390 8621, Japan
    J Bacteriol 184:3194-202. 2002
    ..Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes...
  41. pmc The GrlR-GrlA regulatory system coordinately controls the expression of flagellar and LEE-encoded type III protein secretion systems in enterohemorrhagic Escherichia coli
    Sunao Iyoda
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    J Bacteriol 188:5682-92. 2006
    ..The fact that an EHEC strain constitutively expressing FlhD/FlhC cannot adhere to HeLa cells leads us to hypothesize that GrlA-dependent repression of the flagellar regulon is important for efficient cell adhesion of EHEC to host cells...