Research Topics
Species | Makoto OhnishiSummaryAffiliation: National Institute of Infectious Diseases Country: Japan Publications
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Detail Information
Publications
Identification of TEM-135 beta-lactamase in penicillinase-producing Neisseria gonorrhoeae strains in JapanMakoto Ohnishi
Department of Bacteriology I, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
Antimicrob Agents Chemother 54:3021-3. 2010....- Spread of a chromosomal cefixime-resistant penA gene among different Neisseria gonorrhoeae lineagesMakoto Ohnishi
Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
Antimicrob Agents Chemother 54:1060-7. 2010..Our results provide evidence that the capacity for natural transformation in N. gonorrhoeae plays a role in the spread of chromosomal antibiotic resistance genes and the generation of diversity in such genes... - Transcription of the ehx enterohemolysin gene is positively regulated by GrlA, a global regulator encoded within the locus of enterocyte effacement in enterohemorrhagic Escherichia coliTakehito Saitoh
Department of Bacteriology, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
J Bacteriol 190:4822-30. 2008..These results together suggest that GrlA acts as a positive regulator for the ehx transcription in EHEC... 
Characterization of Shigella sonnei isolates from travel-associated cases in JapanHidemasa Izumiya
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
J Med Microbiol 58:1486-91. 2009..Use of a combination of methods was found to be effective for epidemiological investigation in the case of S. sonnei infection...- Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, ThailandShu ichi Nakayama
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 56:916-20. 2012..gonorrhoeae in the future. This study identified a bla(TEM) variant (bla(TEM-135)) that is a possible intermediate precursor for an ESBL, which warrants international awareness... - Coordinate control of the locus of enterocyte effacement and enterohemolysin genes by multiple common virulence regulators in enterohemorrhagic Escherichia coliSunao Iyoda
Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
Infect Immun 79:4628-37. 2011..Together, these results indicate that transcription of ehx is positively regulated by Ler, GrlA, and LrhA, which all act as positive regulators for LEE expression... - Emergence and genetic diversity of El Tor Vibrio cholerae O1 that possess classical biotype ctxB among travel-associated cases of cholera in JapanMasatomo Morita
Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Med Microbiol 59:708-12. 2010..El Tor variant strains are widespread in Asian countries and show significant genetic diversity, indicating that their spread is a result of multiclonal expansion rather than spread from a single clone... - The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlAKen Shimuta
Department of Bacteriology I, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
BMC Microbiol 9:261. 2009..There have been few studies of the virulence factors of this bacterium. The only S. marcescens hemolytic and cytotoxic factor reported, thus far, is the hemolysin ShlA... - Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificusHidemasa Izumiya
Department of Bacteriology I, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, Japan
Mol Cell Probes 25:174-6. 2011..It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness... - Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio choleraeShouji Yamamoto
Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
J Bacteriol 193:1953-65. 2011..Taking these results together, we propose that in the presence of (GlcNAc)₂, TfoR sRNA is expressed to activate the translation of tfoX(VC), which leads to the induction of natural competence... - New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111Hidemasa Izumiya
Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
Microbiol Immunol 54:569-77. 2010..Because EHEC strains of three major serogroups can be rapidly analyzed on a single platform with our expanded MLVA system, this system could be widely used in molecular epidemiological studies of EHEC infections... - RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion systemJiro Mitobe
Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
EMBO Rep 12:911-6. 2011..Thus, we propose that RodZ is a new membrane-bound RNA-binding protein that provides a scaffold for post-transcriptional regulation... - Molecular and serological investigation of Leptospira and leptospirosis in dogs in JapanNobuo Koizumi
Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Med Microbiol 62:630-6. 2013..This study suggests that the virulence of members of serogroup Hebdomadis in dogs may be associated with the genotypes in this serogroup... - EspO1-2 regulates EspM2-mediated RhoA activity to stabilize formation of focal adhesions in enterohemorrhagic Escherichia coli-infected host cellsTomoko Morita-Ishihara
Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
PLoS ONE 8:e55960. 2013..These results indicated that EspO1-2 interacted with EspM2 to regulate EspM2-mediated RhoA activity and stabilize FA formation during EHEC infection... - Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxoneMakoto Ohnishi
National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 55:3538-45. 2011..Ultimately, the development of new drugs for efficacious gonorrhea treatment is necessary... 
[Gonococcal infection]Ken Shimuta
Department of Bacteriology, National Institute of Infectious Diseases
Nippon Rinsho 65:423-7. 2007- Interferon-γ-producing immature myeloid cells confer protection against severe invasive group A Streptococcus infectionsTakayuki Matsumura
Department of Immunology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
Nat Commun 3:678. 2012..Our results indicate that interferon-γ-producing immature myeloid cells have a protective role during the early stage of severe invasive group A Streptococcus infections... - A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urineNobuo Koizumi
Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan
J Clin Microbiol 50:2072-4. 2012..in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR... - Distribution of monoclonal antibody subgroups and sequence-based types among Legionella pneumophila serogroup 1 isolates derived from cooling tower water, bathwater, and soil in JapanJunko Amemura-Maekawa
Department of Bacteriology I, National Institute of Infectious Diseases, Sinjuku ku, Tokyo, Japan
Appl Environ Microbiol 78:4263-70. 2012..ST1 accounted for 74% of the CT isolates. The only common STs between (i) BW and CT, (ii) BW and SO, and (iii) CT and SO were ST1, ST129, and ST48, respectively, suggesting that each environment constitutes an independent habitat... - Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogensTsuyoshi Sekizuka
Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan
PLoS ONE 6:e25334. 2011..The complete sequence of pNDM-1_Dok01 suggests that the bla(NDM-1) gene was acquired by a novel composite transposon on an extensively disseminated IncA/C plasmid and transferred to the E. coli ST38 isolate... - Outer membrane vesicles of Porphyromonas gingivalis elicit a mucosal immune responseRyoma Nakao
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
PLoS ONE 6:e26163. 2011..In conclusion, the OMVs of P. gingivalis have an important role in mucosal immunogenicity as well as in antigenicity. We propose that P. gingivalis OMV is an intriguing immunogen for development of a periodontal disease vaccine... - Characteristics of Streptococcus suis isolated from patients in JapanBin Chang
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Jpn J Infect Dis 59:397-9. 2006..The remaining isolate was ST28 and its PFGE pattern was distinct from those of the others... - Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El TorMasatomo Morita
National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo, 162 8640, Japan
Microbiol Immunol 52:314-7. 2008..cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1... - Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi AMasatomo Morita
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
Microbiol Immunol 50:707-11. 2006..This assay allows rapid screening of S. enterica serovar Typhi and Paratyphi A with reduced susceptibility to ciprofloxacin... - The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogenyYoshihiko Sakaguchi
Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
Proc Natl Acad Sci U S A 102:17472-7. 2005.... - Whole-genome analysis of Salmonella enterica serovar Typhimurium T000240 reveals the acquisition of a genomic island involved in multidrug resistance via IS1 derivatives on the chromosomeHidemasa Izumiya
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 55:623-30. 2011..enterica serovar Typhimurium. It is expected that future whole-genome studies will aid in the characterization of the horizontal gene transfer events for the emerging S. enterica serovar Typhimurium strains... - Diagnosis of acute leptospirosisTakao Toyokawa
Field Epidemiology Training Program Japan, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
Expert Rev Anti Infect Ther 9:111-21. 2011..will accelerate our understanding of Leptospira pathogenesis and provide insights into the development of more efficient and accurate diagnostic tests for acute-phase leptospirosis... - Meningococcal PilV potentiates Neisseria meningitidis type IV pilus-mediated internalization into human endothelial and epithelial cellsHideyuki Takahashi
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
Infect Immun 80:4154-66. 2012..meningitidis internalization into human cells... - A novel relapsing fever Borrelia sp. infects the salivary glands of the molted hard tick, Amblyomma geoemydaeAi Takano
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
Ticks Tick Borne Dis 3:259-61. 2012..occurred in the tick midgut and the salivary glands, although Borrelia sp. tAG was only detected in the tick midgut. The difference of the borrelial niche in molted ticks might be associated with borrelial characterization... - Competence-dependent endogenous DNA rearrangement and uptake of extracellular DNA give a natural variant of Streptococcus mutans without biofilm formationNaoki Narisawa
Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Bacteriol 193:5147-54. 2011..Our findings may help in understanding how S. mutans can adapt to the oral environment and may explain the evolution of S. mutans... - Multilocus sequence typing implicates rodents as the main reservoir host of human-pathogenic Borrelia garinii in JapanAi Takano
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
J Clin Microbiol 49:2035-9. 2011..Fifty-nine isolates were divided into two phylogenetic groups, and an association was detected between clinical and rodent isolates, suggesting that, in Japan, human-pathogenic B. garinii comes from rodents via ticks... - The GrlR-GrlA regulatory system coordinately controls the expression of flagellar and LEE-encoded type III protein secretion systems in enterohemorrhagic Escherichia coliSunao Iyoda
Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
J Bacteriol 188:5682-92. 2006..The fact that an EHEC strain constitutively expressing FlhD/FlhC cannot adhere to HeLa cells leads us to hypothesize that GrlA-dependent repression of the flagellar regulon is important for efficient cell adhesion of EHEC to host cells... - Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomesTakahiro Murata
Department of Bacteriology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
J Bacteriol 184:3194-202. 2002..Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes...