Hajime Ohgushi

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. pmc Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold
    Koji Hattori
    Department of Orthopaedic Surgery, Nara Medical University, Nara, Japan
    Arthritis Res Ther 7:R552-9. 2005
  2. ncbi request reprint Clinical application of marrow mesenchymal stem cells for hard tissue repair
    Hajime Ohgushi
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki, Hyogo, Japan
    Yonsei Med J 45:61-7. 2004
  3. ncbi request reprint Tissue engineered ceramic artificial joint--ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joints for treatment of osteoarthritis
    Hajime Ohgushi
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki City, Hyogo 661 0974, Japan
    Biomaterials 26:4654-61. 2005
  4. doi request reprint A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow- and adipose tissue-derived mesenchymal stem cells
    Hiroe Ohnishi
    Tissue Engineering Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan
    J Tissue Eng Regen Med 6:261-71. 2012
  5. ncbi request reprint In vivo survival and osteogenic differentiation of allogeneic rat bone marrow mesenchymal stem cells (MSCs)
    Noriko Kotobuki
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki, Hyogo 661 0974, Japan
    Cell Transplant 17:705-12. 2008
  6. ncbi request reprint Observation and quantitative analysis of rat bone marrow stromal cells cultured in vitro on newly formed transparent beta-tricalcium phosphate
    Noriko Kotobuki
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakouji, Amagasaki, Hyogo, 661 0974, Japan
    J Mater Sci Mater Med 17:33-41. 2006
  7. ncbi request reprint Viability and osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells
    Noriko Kotobuki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki, Hyogo, Japan
    Tissue Eng 11:663-73. 2005
  8. doi request reprint Bone augmentation by bone marrow mesenchymal stem cells cultured in three-dimensional biodegradable polymer scaffolds
    Toshimitsu Tanaka
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo, Japan
    J Biomed Mater Res A 91:428-35. 2009
  9. doi request reprint Bone morphogenetic protein-2 in biodegradable gelatin and β-tricalcium phosphate sponges enhances the in vivo bone-forming capability of bone marrow mesenchymal stem cells
    Mika Tadokoro
    Health Research Institute, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    J Tissue Eng Regen Med 6:253-60. 2012
  10. ncbi request reprint Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics
    Noriko Kotobuki
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakouji, Amagasaki, Hyogo 661 0974, Japan
    Biomaterials 26:779-85. 2005

Detail Information

Publications81

  1. pmc Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold
    Koji Hattori
    Department of Orthopaedic Surgery, Nara Medical University, Nara, Japan
    Arthritis Res Ther 7:R552-9. 2005
    ..Therefore, ultrasonic evaluation using a wavelet map can support the evaluation of tissue-engineered cartilage using cell/scaffold complexes...
  2. ncbi request reprint Clinical application of marrow mesenchymal stem cells for hard tissue repair
    Hajime Ohgushi
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki, Hyogo, Japan
    Yonsei Med J 45:61-7. 2004
    ..To date, the tissue engineered cultured bone formed on alumina ceramics in this environment have already been used in clinical situations, such as total ceramic ankle replacements...
  3. ncbi request reprint Tissue engineered ceramic artificial joint--ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joints for treatment of osteoarthritis
    Hajime Ohgushi
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki City, Hyogo 661 0974, Japan
    Biomaterials 26:4654-61. 2005
    ..These preliminary results indicate that the tissue engineering approach using autologous cultured marrow mesenchymal cells might prevent aseptic loosening of the total ankle arthroplasty...
  4. doi request reprint A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow- and adipose tissue-derived mesenchymal stem cells
    Hiroe Ohnishi
    Tissue Engineering Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan
    J Tissue Eng Regen Med 6:261-71. 2012
    ..These results indicate that iPSCs derived from frozen AMSCs/BMSCs exhibit equally acceptable iPSC characteristics and have potential in clinical applications as an alternative source of autogenous stem cells...
  5. ncbi request reprint In vivo survival and osteogenic differentiation of allogeneic rat bone marrow mesenchymal stem cells (MSCs)
    Noriko Kotobuki
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki, Hyogo 661 0974, Japan
    Cell Transplant 17:705-12. 2008
    ....
  6. ncbi request reprint Observation and quantitative analysis of rat bone marrow stromal cells cultured in vitro on newly formed transparent beta-tricalcium phosphate
    Noriko Kotobuki
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakouji, Amagasaki, Hyogo, 661 0974, Japan
    J Mater Sci Mater Med 17:33-41. 2006
    ..This experiment is significant in that all of the results were similarly observed on both the t-beta TCP and PS substrata, indicating the excellent properties of beta TCP ceramics for BMSCs culture towards osteogenic differentiation...
  7. ncbi request reprint Viability and osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells
    Noriko Kotobuki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki, Hyogo, Japan
    Tissue Eng 11:663-73. 2005
    ..In conclusion, cryopreserved as well as noncryopreserved human mesenchymal cells could be applied for bone regeneration in orthopedics...
  8. doi request reprint Bone augmentation by bone marrow mesenchymal stem cells cultured in three-dimensional biodegradable polymer scaffolds
    Toshimitsu Tanaka
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo, Japan
    J Biomed Mater Res A 91:428-35. 2009
    ..These results demonstrate that the three-dimensional PLGA scaffold can support osteogenic differentiation of MSCs, and the scaffold combined with osteogenic MSCs can be used for in vivo bone tissue augmentation...
  9. doi request reprint Bone morphogenetic protein-2 in biodegradable gelatin and β-tricalcium phosphate sponges enhances the in vivo bone-forming capability of bone marrow mesenchymal stem cells
    Mika Tadokoro
    Health Research Institute, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    J Tissue Eng Regen Med 6:253-60. 2012
    ..These results indicate that the combination of MSCs, GT and BMP synergistically enhances osteogenic capability and provides a rational basis for their clinical application in bone reconstruction...
  10. ncbi request reprint Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics
    Noriko Kotobuki
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakouji, Amagasaki, Hyogo 661 0974, Japan
    Biomaterials 26:779-85. 2005
    ..These results confirm the excellent properties of tHA ceramics, which support cell attachment, proliferation, and differentiation. Transparent materials make us know the biological usefulness of ceramics in tissue-engineering field...
  11. doi request reprint In vivo osteogenic capability of human mesenchymal cells cultured on hydroxyapatite and on beta-tricalcium phosphate
    Asako Matsushima
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Amagasaki, Hyogo, Japan
    Artif Organs 33:474-81. 2009
    ....
  12. ncbi request reprint Assessment of viability and osteogenic ability of human mesenchymal stem cells after being stored in suspension for clinical transplantation
    Kaori Muraki
    Tissue Engineering Research Group, Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST Hyogo, Japan
    Tissue Eng 12:1711-9. 2006
    ....
  13. ncbi request reprint Osteogenic differentiation of human dental papilla mesenchymal cells
    Etsuko Ikeda
    National Institute of Advanced Industrial Science and Technology AIST, Research Institute for Cell Engineering RICE, Tissue Engineering Research Group, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    Biochem Biophys Res Commun 342:1257-62. 2006
    ..These results indicate that mesenchymal type cells localize in dental papilla and that the cells can be culture expanded/utilized for bone tissue engineering...
  14. doi request reprint New bone formation by allogeneic mesenchymal stem cell transplantation in a patient with perinatal hypophosphatasia
    Mika Tadokoro
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Hyogo, Japan
    J Pediatr 154:924-30. 2009
    ..We demonstrated the importance of allogeneic MSC transplantation for hypophosphatasia and the constructs as an alternative to bone fragments that provided further osteogenic capability in the patient...
  15. doi request reprint Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC
    Tetsuhiro Aoki
    Tissue Engineering Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki, Hyogo, Japan
    Tissue Eng Part A 16:2197-206. 2010
    ..Further, the colonies were able to differentiate into the three germ layers both in vitro and in vivo. These results show that human iPSCs can be generated by the transduction of three factors (OCT3/4, SOX2, and KLF4) into hASCs...
  16. ncbi request reprint Spatial distribution of mineralized bone matrix produced by marrow mesenchymal stem cells in self-assembling peptide hydrogel scaffold
    Kazunori Hamada
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    J Biomed Mater Res A 84:128-36. 2008
    ..Importantly, the differentiation can occur three dimensionally within the hydrogel, indicating that RADA 16 can be considered attractive synthetic biomaterial for use in bone tissue engineering...
  17. doi request reprint Small interfering RNA of alkaline phosphatase inhibits matrix mineralization
    Noriko Kotobuki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakouji, Amagasaki, Hyogo 661 0974, Japan
    Cell Tissue Res 332:279-88. 2008
    ..These results indicate the usefulness of cloned osteogenic cells in investigating the molecular mechanisms of matrix mineralization, the function of which can be modulated by using a variety of siRNAs...
  18. doi request reprint Effects of epigallocatechin gallate on osteogenic capability of human mesenchymal stem cells after suspension in phosphate-buffered saline
    Kaori Kashiwa
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki, Japan
    Tissue Eng Part A 16:91-100. 2010
    ..These results indicate the usefulness of epigallocatechin gallate treatment for fabrication of ready-to-use cellular products of MSC suspension...
  19. doi request reprint Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities
    Masahiro J Go
    National Institute of Advanced Industrial Science and Technology, Research Institute for Cell Engineering, Tissue Engineering Research Group, Amagasaki, Hyogo, Japan
    Exp Cell Res 314:1147-54. 2008
    ..These results demonstrate not only an effective way to maintain proliferation and differentiation potentials of MSCs but also an important implication about the function of bFGF for self-renewal of stem cells including MSCs...
  20. ncbi request reprint Multipotent cells from the human third molar: feasibility of cell-based therapy for liver disease
    Etsuko Ikeda
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    Differentiation 76:495-505. 2008
    ..These findings suggest that multipotent TGPCs are one of the candidates for cell-based therapy to treat liver diseases and offer unprecedented opportunities for developing therapies in treating tissue repair and regeneration...
  21. ncbi request reprint Osteogenic differentiation of cultured rat and human bone marrow cells on the surface of zinc-releasing calcium phosphate ceramics
    Masako Ikeuchi
    National Institute of Advanced Industrial Science and Technology AIST, Tissue Engineering Research Center TERC, 3 11 46 Nakouji, Amagasaki, Hyougo 661 0974, Japan
    J Biomed Mater Res A 67:1115-22. 2003
    ....
  22. ncbi request reprint Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow
    Noriko Kotobuki
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Nakoji, Amagasaki, Hyogo, Japan
    Artif Organs 28:33-9. 2004
    ..Moreover, the MSCs could be cryopreserved at -80 degrees C without noticeable loss of viability and capability of osteoblastic differentiation. These results indicate that MSCs hold promise for utilization in hard tissue regeneration...
  23. ncbi request reprint Growth factor combination for chondrogenic induction from human mesenchymal stem cell
    Nitaya Indrawattana
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    Biochem Biophys Res Commun 320:914-9. 2004
    ..The combination of these growth factors may be the important key for in vitro chondrogenesis induction...
  24. ncbi request reprint Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells
    Takanori Kihara
    Tissue Engineering Research Center TERC, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    Biochem Biophys Res Commun 316:943-8. 2004
    ....
  25. ncbi request reprint Enhancement of in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblast-like cells
    Noriko Kotobuki
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Nakoji 3 11 46, Amagasaki, Hyogo 661 0974, Japan
    Cell Transplant 13:377-83. 2004
    ..These results demonstrated that using the sorting technique to isolate the high AP cells might be a useful method for applications in bone tissue engineering...
  26. ncbi request reprint Pharmacological characterization of isoproterenol-treated medaka fish
    Takashi Kawasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46, Nakoji, Amagasaki 661 0974, Japan
    Pharmacol Res 58:348-55. 2008
    ....
  27. ncbi request reprint Rapid preparation of fresh frozen tissue-engineered bone sections for histological, histomorphological and histochemical analyses
    Mika Tadokoro
    National Institute of Advanced Industrial Science and Technology, Amagasaki Site, 3 11 46, Wakaouji, Amagasaki, Hyogo 661 0974, Japan
    Biomed Mater Eng 16:405-13. 2006
    ..It is expected that this method will become a powerful tool in tissue-engineering of hard tissues...
  28. ncbi request reprint Mesenchymal stem cells and bioceramics: strategies to regenerate the skeleton
    Hajime Ohgushi
    Tissue Engineering Research Center TERC, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakouji, Amagasaki City, Hyogo 661 0794, Japan
    Novartis Found Symp 249:118-27; discussion 127-32, 170-4, 239-41. 2003
    ..We have begun studying this tissue-engineering approach in patients with skeletal problems. This paper describes this and other approaches using MSCs to regenerate skeletal tissue...
  29. doi request reprint Correlation between proliferative activity and cellular thickness of human mesenchymal stem cells
    Yoshihiro Katsube
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakoji, Amagasaki, Hyogo 661 0974, Japan
    Biochem Biophys Res Commun 368:256-60. 2008
    ..These findings suggest that the measurement of cellular thickness is useful for estimating the proliferative activity of human MSCs and is expected to be a practical tool for MSC applications in regenerative medicine...
  30. pmc Induction of pluripotent stem cells from human third molar mesenchymal stromal cells
    Yasuaki Oda
    Tissue Engineering Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology, Hyogo 661 0974, Japan
    J Biol Chem 285:29270-8. 2010
    ..Because human third molars are discarded as clinical waste, our data indicate that clonally expanded MSCs derived from human third molars are a valuable cell source for the generation of iPS cells...
  31. ncbi request reprint High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy
    Sung Woong Han
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Tokyo, Japan
    Nanomedicine 4:215-25. 2008
    ..The highest rate of expressed GFP per delivered DNA was achieved using the nanoneedle, because the nanoneedle could be inserted into the nucleus directly without causing significant cell damage...
  32. ncbi request reprint Effect of forced expression of basic fibroblast growth factor in human bone marrow-derived mesenchymal stromal cells
    Masahiro J Go
    National Institute of Advanced Industrial Science and Technology, Research Institute for Cell Engineering, Tissue Engineering Research Group, Nakouji 3 11 46, Amagasaki, Hyogo 661 0974, Japan
    J Biochem 142:741-8. 2007
    ....
  33. doi request reprint Human mesenchymal stem cells as a stable source of VEGF-producing cells
    Harumi Kagiwada
    Tissue Engineering Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46 Nakoji, Amagasaki, Hyogo, Japan
    J Tissue Eng Regen Med 2:184-9. 2008
    ..These findings suggest that implantation of hMSCs is a practical means as a source of VEGF production and might be effective in neoangiogenesis...
  34. ncbi request reprint Recombinant human bone morphogenetic protein-2 promotes osteogenesis within atelopeptide type I collagen solution by combination with rat cultured marrow cells
    Masako Ikeuchi
    Department of Oral and Maxillofacial Surgery, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8522, Japan
    J Biomed Mater Res 60:61-9. 2002
    ..Therefore, this study suggests that clinical repairs of bone defects are feasible using injectable AC solution with rhBMP-2 and cultured MSCs as a bone substitute...
  35. doi request reprint Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue
    Ousuke Hayashi
    Department of Orthopedic Surgery, Nippon Medical School, Tokyo, Japan
    Calcif Tissue Int 82:238-47. 2008
    ..001). These results indicate that BMSCs and PMSCs could be ideal candidates for utilization in practical bone tissue regeneration...
  36. doi request reprint Mesenchymal stromal cells improve the osteogenic capabilities of mineralized agarose gels in a rat full-thickness cranial defect model
    Norihiko Mizuta
    Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, 1 8 Yamadaoka, Suita, Osaka, 565 0871, Japan
    J Tissue Eng Regen Med 7:51-60. 2013
    ..As a result, MSC-loaded gels could represent viable therapeutic biomaterials for bone tissue engineering...
  37. pmc Spectrocolorimetric evaluation of repaired articular cartilage after a microfracture
    Koji Hattori
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki Site, Amagasaki, Hyogo, Japan
    BMC Res Notes 1:87. 2008
    ..Objective assessments are required. A spectrocolorimeter was used to evaluate whether intact and repaired cartilage can be quantified...
  38. doi request reprint Osteogenic activity of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on irradiated allogenic bone
    Yasuaki Tohma
    Department of Orthopaedic Surgery, Nara Medical University, Nara, Japan
    J Tissue Eng Regen Med 6:96-102. 2012
    ..Our present tissue-engineering method might contribute to a wide variety of allogenic bone grafting techniques in clinical settings...
  39. pmc Formation of microvilli and phosphorylation of ERM family proteins by CD43, a potent inhibitor for cell adhesion: cell detachment is a potential cue for ERM phosphorylation and organization of cell morphology
    Junko Yamane
    Health Research Institute, Tissue Engineering Research Group, National Institute of Advanced Industrial Science and Technology, Nakouji, Amagasaki, Hyogo, Japan
    Cell Adh Migr 5:119-32. 2011
    ..Furthermore, our study suggests a novel possibility that cell detachment itself induces activation of ERM and modification of cell shape...
  40. doi request reprint In vivo study of dendronlike nanoparticles for stem cells "tune-up": from nano to tissues
    Joaquim M Oliveira
    Biomaterials, Biodegradables and Biomimetics Research Group, Department of Polymer Engineering, University of Minho, Headquarters of European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimaraes, Portugal
    Nanomedicine 7:914-24. 2011
    ..Favorable results were observed in vivo, thus suggesting that stem cell "tune-up" strategy can open up a new regenerative strategy for bone-tissue engineering...
  41. doi request reprint Use of BAC array CGH for evaluation of chromosomal stability of clinically used human mesenchymal stem cells and of cancer cell lines
    Soichiro Saito
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Japan
    Hum Cell 24:2-8. 2011
    ..Therefore, BAC aCGH is highly recommended for use for a quality check of various cells before using them for any kind of biological investigation or clinical application...
  42. doi request reprint N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation
    Hisako Ishimine
    Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biochem Biophys Res Commun 438:753-9. 2013
    ..5, Hand1, and GATA4 mRNAs. Our results suggest that N-cadherin is a novel prospective cell surface marker of human MSCs that show a better ability for cardiomyocyte differentiation. ..
  43. pmc Mechanical effects of surgical procedures on osteochondral grafts elucidated by osmotic loading and real-time ultrasound
    Koji Hattori
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3 11 46, Nakoji, Amagasaki, Hyogo 661 0974, Japan
    Arthritis Res Ther 11:R134. 2009
    ..The present study aimed to investigate the mechanical effects of osteochondral plug implantation using osmotic loading and real-time ultrasound...
  44. doi request reprint Molecular cloning and gene expression of the prox1a and prox1b genes in the medaka, Oryzias latipes
    Tomonori Deguchi
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, 3 11 46 Nakouji, Amagasaki, Hyogo 661 0974, Japan
    Gene Expr Patterns 9:341-7. 2009
    ..The expression pattern of prox1a showed a pattern similar to that of zebrafish prox1. In contrast, medaka prox1b was expressed asymmetrically in part of the central nervous system, especially strongly in the right side of the habenula...
  45. doi request reprint Osteogenic matrix sheet-cell transplantation using osteoblastic cell sheet resulted in bone formation without scaffold at an ectopic site
    Manabu Akahane
    Department of Orthopaedic Surgery, Nara Medical University School of Medicine, Nara, Japan
    J Tissue Eng Regen Med 2:196-201. 2008
    ..Our present results indicate that MSCs can be cultured as sheet structures, and the resulting sheets themselves or HA-sheet composites represent osteogenic implants that can be used for hard tissue reconstruction...
  46. ncbi request reprint Bone tissue engineering using novel interconnected porous hydroxyapatite ceramics combined with marrow mesenchymal cells: quantitative and three-dimensional image analysis
    Masataka Nishikawa
    Department of Orthopaedics, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita City, Osaka 565 0871, Japan
    Cell Transplant 13:367-76. 2004
    ..The results indicate that a combination of HA150 and mesenchymal cells could be used as an excellent bone graft substitute because of its mechanical properties and capability of inducing bone formation...
  47. doi request reprint Osteogenesis depending on geometry of porous hydroxyapatite scaffolds
    Masataka Yoshikawa
    Department of Endodontics, Osaka Dental University, Osaka, Japan
    Calcif Tissue Int 83:139-45. 2008
    ..It is considered that laminin may act as an adhesive for effective cell attachment to the walls of the pores in an HA scaffold...
  48. ncbi request reprint Enhancing and suppressing effects of dexamethasone on transgene expression in vitro
    Manabu Akahane
    Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara 634 8522, Japan
    Int J Mol Med 10:107-12. 2002
    ..These results indicate that dexamethasone may play an important role for achieving successful gene therapy. Furthermore, these observations may also have important implications for future clinical applications of gene therapy...
  49. ncbi request reprint Osteogenic potential of cultured bone/ceramic construct: comparison with marrow mesenchymal cell/ceramic composite
    Jin Iida
    Department of Orthopaedic Surgery, Nara Medical University, Kashihara City, Nara 634 8522, Japan
    Cell Transplant 13:357-65. 2004
    ..Histological examination and gene expression data agreed with these findings. The culture technique discussed herein should facilitate the development of biosynthetic bone implants with higher osteogenic capacity...
  50. ncbi request reprint Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold
    Guoping Chen
    Biomaterials Center, National Institute for Materials Science, 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
    Biochem Biophys Res Commun 322:50-5. 2004
    ..The PLGA-collagen hybrid mesh enabled the aggregation of mesenchymal stem cells and provided a promotive microenvironment for the chondrogenic differentiation of the MSCs...
  51. ncbi request reprint The effect of simulated microgravity by three-dimensional clinostat on bone tissue engineering
    Masataka Nishikawa
    Department of Orthopaedics, Osaka University Graduate School of Medicine, Suita City, Japan
    Cell Transplant 14:829-35. 2005
    ..These results indicate that new bone formation in culture was inhibited by use of the 3D clinostat, and that this inhibition was mainly due to suppression of osteoblastic differentiation of MMCs...
  52. ncbi request reprint Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis
    Noritoshi Nagaya
    Dept of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, 5 7 1 Fujishirodai, Suita, Osaka 565 8565, Japan
    Am J Physiol Heart Circ Physiol 287:H2670-6. 2004
    ..05 vs. control). These results suggest that intravenous administration of MSCs improves cardiac function after acute myocardial infarction through enhancement of angiogenesis and myogenesis in the ischemic myocardium...
  53. ncbi request reprint Bone regeneration by grafting of cultured human bone
    Takafumi Yoshikawa
    Department of Pathology, Nara Medical University Kashihara City, Nara, Japan
    Tissue Eng 10:688-98. 2004
    ....
  54. ncbi request reprint Potentiation of the activity of bone morphogenetic protein-2 in bone regeneration by a PLA-PEG/hydroxyapatite composite
    Takashi Kaito
    Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Biomaterials 26:73-9. 2005
    ....
  55. ncbi request reprint Hydroxyapatite ceramics as a carrier of gene-transduced bone marrow cells
    Manabu Akahane
    Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan
    J Orthop Sci 7:677-82. 2002
    ..Because of the durability of the newly formed bone tissue, creating composites of cultured bone marrow cells and hydroxyapatite ceramics might be an ideal method for exogenous gene transfection...
  56. ncbi request reprint Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics
    Shigeyuki Kitamura
    Department of Orthopedic Surgery, Nippon Medical School, Tokyo, Japan
    Artif Organs 28:72-82. 2004
    ..The cultured bone/alumina composites should prevent the aseptic loosening of all-alumina ceramic joints or the detachment of implanted alumina ceramics, and thus could have clinical significance in orthopedic reconstructive surgery...
  57. ncbi request reprint Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal stem cells in porous hydroxyapatite ceramic
    Hideki Shimaoka
    Department of Public Health, Nara Medical University, 840 Shijocho, Kashihara, Nara 634 8521, Japan
    J Biomed Mater Res A 68:168-76. 2004
    ..The results indicate that GDF-5 synergistically enhances de novo bone formation capability of MSCs/HA composite and suggest that tissue-engineered GDF/MSCs/HA composites could be used as bone graft substitutes...
  58. ncbi request reprint [Regenerative medicine for bone and joint]
    Yoshinori Takakura
    Department of Orthopaedic Surgery, Nara Medical University
    Nihon Rinsho 66:971-7. 2008
    ..The follow-up periods are not so long, because they were started from 2001. The good results of them without rejection and infection have been obtained for the present...
  59. ncbi request reprint Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh
    Guoping Chen
    Biomaterials Center, National Institute for Materials Science, 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
    Biomaterials 26:2559-66. 2005
    ..PLGA-collagen hybrid mesh may be useful for skin tissue engineering...
  60. ncbi request reprint Transplantation of mesenchymal stem cells improves cardiac function in a rat model of dilated cardiomyopathy
    Noritoshi Nagaya
    Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Osaka 565 8565, Japan
    Circulation 112:1128-35. 2005
    ..However, little information is available about the therapeutic potency of MSC transplantation in cases of dilated cardiomyopathy (DCM), an important cause of heart failure...
  61. ncbi request reprint In vitro mineralization by mesenchymal stem cells cultured on titanium scaffolds
    Masahiko Maeda
    Department of Oral and Maxillofacial Surgery, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    J Biochem 141:729-36. 2007
    ..Therefore, the titanium is an effective scaffold that is applicable in bone reconstruction surgery...
  62. ncbi request reprint Rapamycin as an inhibitor of osteogenic differentiation in bone marrow-derived mesenchymal stem cells
    Shinji Isomoto
    Department of Orthopaedic Surgery, Nara Medical University, 840 Shijyo cho, Kashihara, Nara, 634 8522, Japan
    J Orthop Sci 12:83-8. 2007
    ..This study assessed the effects of immunosuppressant drugs on osteogenic differentiation of mesenchymal stem cells and analyzed the manner in which immunosuppressant drugs modulate the osteogenic effect of dexamethasone...
  63. ncbi request reprint Tissue-engineered approach for the treatment of steroid-induced osteonecrosis of the femoral head: transplantation of autologous mesenchymal stem cells cultured with beta-tricalcium phosphate ceramics and free vascularized fibula
    Kenji Kawate
    Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Nara, Japan
    Artif Organs 30:960-2. 2006
    ..This tissue-engineered approach has potentials for the treatment of osteonecrosis. However, our results suggested that the present procedure could not be used for cases with severe preoperative collapse...
  64. ncbi request reprint Tissue engineering approach to the treatment of bone tumors: three cases of cultured bone grafts derived from patients' mesenchymal stem cells
    Toru Morishita
    Department of Orthopedic Surgery, National Hospital Organization Nara Medical Center, Nara, Japan
    Artif Organs 30:115-8. 2006
    ..The results indicate that tissue-engineered osteogenic ceramics might be an alternative to autologous bone grafts...
  65. ncbi request reprint Experimental study of vascularized tissue-engineered bone grafts
    Kenji Kawamura
    Department of Orthopedic Surgery, Nara Medical University, Kashihara, Japan
    Plast Reconstr Surg 117:1471-9. 2006
    ..This method has disadvantages, however, such as donor-site complications. To overcome these disadvantages, the authors developed a new method for creating vascularized tissue-engineered bone grafts in an experimental model...
  66. ncbi request reprint Influence of the porosity of hydroxyapatite ceramics on in vitro and in vivo bone formation by cultured rat bone marrow stromal cells
    Masanori Okamoto
    Department of Otolaryngology, Nara Medical University School of Medicine, Kashihara, Japan
    J Mater Sci Mater Med 17:327-36. 2006
    ..In this in vivo model, different scaffold porosity with similar crystal morphology of the apatite phase demonstrated marked differences in ability to support osteogenesis by implanted rat MSCs...
  67. ncbi request reprint Exogenous type I collagen facilitates osteogenic differentiation and acts as a substrate for mineralization of rat marrow mesenchymal stem cells in vitro
    Takanori Kihara
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3 8 1 Komaba, Meguro ku, Tokyo 153 8902, Japan
    Biochem Biophys Res Commun 341:1029-35. 2006
    ....
  68. ncbi request reprint Early bone in-growth ability of alumina ceramic implants loaded with tissue-engineered bone
    Yasuaki Tohma
    Department of Orthopedic Surgery, Nara Medical University, 840 Shijocho, Kashihara, Nara 634 8521, Japan
    J Orthop Res 24:595-603. 2006
    ..The present findings indicate early bonding between the implant and bone three weeks after the procedure...
  69. ncbi request reprint Mesenchymal stem cells for the treatment of heart failure
    Shunsuke Ohnishi
    Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Osaka, Japan
    Int J Hematol 86:17-21. 2007
    ..On the basis of information obtained from basic and translational research, several clinical trials have recently been started to evaluate the safety and efficacy of autologous MSC for heart failure...
  70. ncbi request reprint Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CCl4-injured rats
    Suguru Oyagi
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamada oka, Suita, Osaka 565 0871 Japan
    J Hepatol 44:742-8. 2006
    ..Here, we transplanted bone marrow-derived mesenchymal cells (BMMCs) to test their effect in liver-injured rats...
  71. ncbi request reprint Repair of articular cartilage defects in the patello-femoral joint with autologous bone marrow mesenchymal cell transplantation: three case reports involving nine defects in five knees
    Shigeyuki Wakitani
    Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, Osaka, Japan
    J Tissue Eng Regen Med 1:74-9. 2007
    ..Autologous bone marrow mesenchymal cells transplantation may be an effective approach to promote the repair of articular cartilage defects...
  72. ncbi request reprint Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2)
    Naoki Maegawa
    Department of Orthopaedic Surgery, Nara Medical University, 840 Shijocho, Kashihara, Nara 634 8521, Japan
    J Tissue Eng Regen Med 1:306-13. 2007
    ..The sequential supplementation of these cytokines into MSC culture might be suitable for the fabrication of ideal cultured bone for use in bone tissue engineering...
  73. ncbi request reprint Availability of bone marrow stromal cells in three-dimensional coculture with hepatocytes and transplantation into liver-damaged mice
    Masashi Takeda
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biosci Bioeng 100:77-81. 2005
    ..Thus, the transplantation of BMSCs appears to have a systemic effect on recipients through the increase in the serum cytokine level as well as a local effect on cotransplanted hepatocytes...
  74. doi request reprint Bone marrow-derived mesenchymal cells can rescue osteogenic capacity of devitalized autologous bone
    Yasuaki Tohma
    Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Japan
    J Tissue Eng Regen Med 2:61-8. 2008
    ..The benefits would be applicable to all types of devitalized bone...
  75. ncbi request reprint [Application of mesenchymal stem cells to liver regenerative medicine]
    Kiyohito Yagi
    Graduate School of Pharmaceutical Sciences, Osaka University, Suita City, Japan
    Yakugaku Zasshi 128:3-9. 2008
    ..Autologous transplantation of mesenchymal stem cells from bone marrow and dental papilla could be ethically and functionally promising for stem cell-based therapy...
  76. ncbi request reprint Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold
    Kota Uematsu
    Department of Orthopaedic Surgery, Nara Medical University, 840, Shijou cho, Kashihara City, Nara 634 8522, Japan
    Biomaterials 26:4273-9. 2005
    ..The structure of the novel PLGA scaffolds provided architectural support for the differentiation of progenitor cells and demonstrated successful induction of in vivo chondrogenesis...
  77. ncbi request reprint Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture
    Yoshiko Dohi
    Department of Public Health, Nara Medical University, Kashihara City, Japan
    Biol Trace Elem Res 104:57-70. 2005
    ..The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation...
  78. ncbi request reprint [Repair of arthritic lesions using cultured mesenchymal cells and artificial joints]
    Noriko Kotobuki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology
    Nihon Rinsho 63:680-5. 2005
  79. ncbi request reprint Novel ultrasonic evaluation of tissue-engineered cartilage for large osteochondral defects--non-invasive judgment of tissue-engineered cartilage
    Koji Hattori
    Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Nara 634 8522, Japan
    J Orthop Res 23:1179-83. 2005
    ....
  80. ncbi request reprint Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone
    Yoshiko Dohi
    Department of Public Health, Nara Medical University, 840 Shijocho, Kashihara, Nara 634 8521, Japan
    Biochimie 86:471-80. 2004
    ....
  81. ncbi request reprint Apatite formed on/in agarose gel as a bone-grafting material in the treatment of periodontal infrabony defect
    Masashi Tabata
    Department of Oral and Maxillofacial Surgery, Miyakonojo National Hospital, 5033 1 Iwayoshi, Miyakonojo, Miyazaki 885 0014, Japan
    J Biomed Mater Res B Appl Biomater 75:378-86. 2005
    ..Six months postoperatively, inflammatory granulation tissue was observed around the particles. The present study suggests that HA/agarose gels may play an important role in the regeneration of lost periodontal tissue...