Tomoyoshi Nozaki

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. ncbi request reprint [Sulfur-containing amino acid metabolism in parasitic protozoa]
    Tomoyoshi Nozaki
    Tanpakushitsu Kakusan Koso 47:21-9. 2002
  2. ncbi request reprint Sulfur-containing amino acid metabolism in parasitic protozoa
    Tomoyoshi Nozaki
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma, 371 8511, Japan
    Adv Parasitol 60:1-99. 2005
  3. pmc Current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by "amitochondriate" protozoan parasites
    Vahab Ali
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Clin Microbiol Rev 20:164-87. 2007
  4. doi request reprint Kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis
    Dan Sato
    Department of Parasitology, Gunma University Graduate School of Medicine, Japan
    FEBS J 275:548-60. 2008
  5. pmc Novel transmembrane receptor involved in phagosome transport of lysozymes and β-hexosaminidase in the enteric protozoan Entamoeba histolytica
    Atsushi Furukawa
    Department of Parasitology, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, Japan
    PLoS Pathog 8:e1002539. 2012
  6. pmc Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation
    Ghulam Jeelani
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    PLoS ONE 7:e37740. 2012
  7. pmc A novel class of cysteine protease receptors that mediate lysosomal transport
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Cell Microbiol 14:1299-317. 2012
  8. pmc Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS Negl Trop Dis 6:e1831. 2012
  9. pmc Trend of Entamoeba histolytica infestation in Kolkata
    Avik K Mukherjee
    Department of Parasitology, National Institute of Cholera and Enteric Diseases, Indian Council of Medical Research, P33 CIT Road, Scheme XM, Kolkata 700010, India
    Gut Pathog 2:12. 2010
  10. ncbi request reprint Cloning and characterization of a gene encoding phosphatidyl inositol-specific phospholipase C from Trypanosoma cruzi
    T Nozaki
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    Mol Biochem Parasitol 102:283-95. 1999

Collaborators

Detail Information

Publications60

  1. ncbi request reprint [Sulfur-containing amino acid metabolism in parasitic protozoa]
    Tomoyoshi Nozaki
    Tanpakushitsu Kakusan Koso 47:21-9. 2002
  2. ncbi request reprint Sulfur-containing amino acid metabolism in parasitic protozoa
    Tomoyoshi Nozaki
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma, 371 8511, Japan
    Adv Parasitol 60:1-99. 2005
    ....
  3. pmc Current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by "amitochondriate" protozoan parasites
    Vahab Ali
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Clin Microbiol Rev 20:164-87. 2007
    ..We further discuss our approach to exploit unique sulfur-containing-amino-acid metabolism, focusing on development of drugs against E. histolytica...
  4. doi request reprint Kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis
    Dan Sato
    Department of Parasitology, Gunma University Graduate School of Medicine, Japan
    FEBS J 275:548-60. 2008
    ..These results support the use of TFM to target MGL...
  5. pmc Novel transmembrane receptor involved in phagosome transport of lysozymes and β-hexosaminidase in the enteric protozoan Entamoeba histolytica
    Atsushi Furukawa
    Department of Parasitology, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, Japan
    PLoS Pathog 8:e1002539. 2012
    ..This work represents the first case of the identification of a transport receptor of hydrolytic enzymes responsible for the degradation of microorganisms in phagosomes...
  6. pmc Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation
    Ghulam Jeelani
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    PLoS ONE 7:e37740. 2012
    ....
  7. pmc A novel class of cysteine protease receptors that mediate lysosomal transport
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Cell Microbiol 14:1299-317. 2012
    ..histolytica...
  8. pmc Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS Negl Trop Dis 6:e1831. 2012
    ..We proposed the significance of the glycerol biosynthetic pathway as a metabolic anti-oxidative defense system in E. histolytica...
  9. pmc Trend of Entamoeba histolytica infestation in Kolkata
    Avik K Mukherjee
    Department of Parasitology, National Institute of Cholera and Enteric Diseases, Indian Council of Medical Research, P33 CIT Road, Scheme XM, Kolkata 700010, India
    Gut Pathog 2:12. 2010
    ..Entamoeba histolytica infection is found almost all over the world and is highly endemic and a major cause of parasitic diarrhoea particularly in the developing countries...
  10. ncbi request reprint Cloning and characterization of a gene encoding phosphatidyl inositol-specific phospholipase C from Trypanosoma cruzi
    T Nozaki
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    Mol Biochem Parasitol 102:283-95. 1999
    ..The yeast-expressed T. cruzi PLC showed an absolute Ca2+ dependence which was similar to mammalian PLC isoforms: the half-maximal activity at 0.5-1 x 10(-5) M Ca2+ and the maximal activity at 1-2 x 10(-4) M Ca2+...
  11. ncbi request reprint Current problems of amebiasis in Japan and recent advances in amebiasis research
    T Nozaki
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Jpn J Infect Dis 53:229-37. 2000
    ....
  12. ncbi request reprint Characterization of the gene encoding serine acetyltransferase, a regulated enzyme of cysteine biosynthesis from the protist parasites Entamoeba histolytica and Entamoeba dispar. Regulation and possible function of the cysteine biosynthetic pathway in Ent
    T Nozaki
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 274:32445-52. 1999
    ..These results indicate that the cysteine biosynthetic pathway plays an important role in antioxidative defense of these enteric parasites...
  13. ncbi request reprint Characterization of transsulfuration and cysteine biosynthetic pathways in the protozoan hemoflagellate, Trypanosoma cruzi. Isolation and molecular characterization of cystathionine beta-synthase and serine acetyltransferase from Trypanosoma
    T Nozaki
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 276:6516-23. 2001
    ..Together, these studies demonstrate the T. cruzi is a unique protist in possessing both transsulfuration and sulfur assimilatory pathways...
  14. doi request reprint Biochemical and functional characterization of novel NADH kinase in the enteric protozoan parasite Entamoeba histolytica
    Ghulam Jeelani
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Biochimie 95:309-19. 2013
    ..These data, together with the essentially of NAD(H) kinase gene, underscore its significance as an NADP(H)-producing enzyme in this organism, and should help in designing of drugs targeting this enzyme...
  15. pmc Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 285:39160-70. 2010
    ..To date, this study represents the first case where L-cysteine deprivation leads to drastic changes in core metabolic pathways, including energy, amino acid, and phospholipid metabolism...
  16. doi request reprint Characterization of two isotypes of l-threonine dehydratase from Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Mol Biochem Parasitol 170:100-4. 2010
    ..Together, these data showed that EhTD1 possesses unique regulatory properties distinct from other organisms and may play an important role in energy metabolism via amino acid degradation in E. histolytica...
  17. ncbi request reprint Kinetics and strain variation of phagosome proteins of Entamoeba histolytica by proteomic analysis
    Mami Okada
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Mol Biochem Parasitol 145:171-83. 2006
    ..These data should provide significant insights into molecular characterization of phagosome biogenesis, and help to elucidate the pathogenesis of this important infection...
  18. ncbi request reprint Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica
    Biswa Nath Mitra
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 851, Japan
    Cell Microbiol 9:2112-25. 2007
    ..This is the first report demonstrating that Rab11 plays a central role in the transport and secretion of pathogenic factors...
  19. ncbi request reprint Two Rab7 isotypes, EhRab7A and EhRab7B, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite Entamoeba histolytica
    Yumiko Saito-Nakano
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Tokyo 162 8640, Japan
    Cell Microbiol 9:1796-808. 2007
    ..Altogether, these data indicate that the two Rab7 isotypes play distinct but co-ordinated roles in lysosome and phagosome biogenesis...
  20. doi request reprint Conservation and function of Rab small GTPases in Entamoeba: annotation of E. invadens Rab and its use for the understanding of Entamoeba biology
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Exp Parasitol 126:337-47. 2010
    ..invadens Rab genes to understand the ubiquitous importance of Rab-mediated membrane trafficking during important biological processes including differentiation in Entamoeba...
  21. pmc Two atypical L-cysteine-regulated NADPH-dependent oxidoreductases involved in redox maintenance, L-cystine and iron reduction, and metronidazole activation in the enteric protozoan Entamoeba histolytica
    Ghulam Jeelani
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 285:26889-99. 2010
    ....
  22. ncbi request reprint Entamoeba histolytica: differences in phagosome acidification and degradation between attenuated and virulent strains
    Biswa Nath Mitra
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 851, Japan
    Exp Parasitol 114:57-61. 2006
    ..69 pH/min), persisted at 5.11+/-0.23, and degraded GFP less efficiently (60-71% decrease). These results suggest that efficiency of phagosome maturation is most probably inversely correlated with apparent virulence...
  23. ncbi request reprint Mechanism of trifluoromethionine resistance in Entamoeba histolytica
    Gil M Penuliar
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Antimicrob Chemother 66:2045-52. 2011
    ..To determine the mechanism of trifluoromethionine resistance in Entamoeba histolytica and evaluate the impact of acquired drug resistance on virulence...
  24. doi request reprint Identification of an avirulent Entamoeba histolytica strain with unique tRNA-linked short tandem repeat markers
    Aleyla Escueta-de Cadiz
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Parasitol Int 59:75-81. 2010
    ..histolytica strains exist, that are associated with unique genotypes. Furthermore, STR genotyping and in vivo challenge of 2 other asymptomatic isolates (KU14 and KU26) verified the covert virulence of these strains...
  25. doi request reprint Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica
    Gil M Penuliar
    Department of Parasitology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    J Antimicrob Chemother 67:375-86. 2012
    ..The transcriptional response, however, of this parasite to the drug is not known. In this study, we used microarray analysis to determine whether additional genes are involved...
  26. pmc Transcriptome analysis of encystation in Entamoeba invadens
    Aleyla Escueta De Cádiz
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan Department of Biological Science and Environmental Studies, College of Science and Mathematics, University of the Philippines Mindanao, Davao, Philippines
    PLoS ONE 8:e74840. 2013
    ....
  27. pmc Novel TPR-containing subunit of TOM complex functions as cytosolic receptor for Entamoeba mitosomal transport
    Takashi Makiuchi
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Sci Rep 3:1129. 2013
    ..Our data indicate that Entamoeba has invented a novel lineage-specific shuttle receptor of the TOM complex as a consequence of adaptation to an anaerobic environment...
  28. doi request reprint Isoform-dependent feedback regulation of serine O-acetyltransferase isoenzymes involved in L-cysteine biosynthesis of Entamoeba histolytica
    Sarwar Hussain
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi 371 8511, Japan
    Mol Biochem Parasitol 163:39-47. 2009
    ..histolytica under different conditions, e.g. during colonization of the intestine and tissue invasion...
  29. ncbi request reprint Two cysteine protease inhibitors, EhICP1 and 2, localized in distinct compartments, negatively regulate secretion in Entamoeba histolytica
    Dan Sato
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    FEBS Lett 580:5306-12. 2006
    ..These data indicate that the two EhICPs, present in distinct subcellular compartments, negatively regulate CP secretion, and, thus, the virulence of this parasite...
  30. pmc Molecular and structural characterization of NADPH-dependent d-glycerate dehydrogenase from the enteric parasitic protist Entamoeba histolytica
    Vahab Ali
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biochem J 375:729-36. 2003
    ..We propose that the GDH enzyme is likely to be involved in regulation of the intracellular concentration of serine, and, thus, also in controlling cysteine biosynthesis located downstream of serine metabolic pathways in this protist...
  31. pmc Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica
    Mohammad Abu Yousuf
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo, Japan
    Eukaryot Cell 9:926-33. 2010
    ..PNT that is compartmentalized to the acidic compartments is unprecedented in eukaryotes and may possess a unique physiological role in E. histolytica...
  32. pmc Geographic diversity among genotypes of Entamoeba histolytica field isolates
    Ali Haghighi
    Department of Parasitology, National Institute of Infectious Diseases, Keio University School of Medicine, Shinjuku ku, Japan
    J Clin Microbiol 41:3748-56. 2003
    ..g., colitis or dysentery and liver abscess, no association between the clinical presentation and the SREHP genotype at either the nucleotide or the predicted amino acid level was demonstrated...
  33. pmc Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    BMC Genomics 12:275. 2011
    ..Recently, we demonstrated that in E. histolytica, L-cysteine regulates various metabolic pathways including energy, amino acid, and phospholipid metabolism...
  34. ncbi request reprint Differences in morphology of phagosomes and kinetics of acidification and degradation in phagosomes between the pathogenic Entamoeba histolytica and the non-pathogenic Entamoeba dispar
    Biswa Nath Mitra
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371 851, Japan
    Cell Motil Cytoskeleton 62:84-99. 2005
    ..histolytica, but not in E. dispar. These data suggest the presence of biochemical or functional differences in the involvement of microtubules and proteases in phagosome maturation and degradation between the two species...
  35. pmc Cysteine protease-binding protein family 6 mediates the trafficking of amylases to phagosomes in the enteric protozoan Entamoeba histolytica
    Atsushi Furukawa
    Department of Parasitology, National Institute of Infectious Diseases, Toyama, Tokyo, Japan
    Infect Immun 81:1820-9. 2013
    ..We also demonstrated that the carboxyl-terminal cytosolic region of CPBF6 is engaged in the regulation of the trafficking of CPBF6 to phagosomes. Our proteome analysis of phagosomes also revealed new potential phagosomal proteins...
  36. ncbi request reprint Molecular and biochemical characterization of D-phosphoglycerate dehydrogenase from Entamoeba histolytica. A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways
    Vahab Ali
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    Eur J Biochem 271:2670-81. 2004
    ..Amino acid sequence comparison and phylogenetic analysis of various PGDH sequences showed that E. histolytica forms a highly supported monophyletic group with another enteric protozoa, cilliate E. caudatum, and bacteroides...
  37. pmc A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi 371 8511, Japan
    Mol Biol Cell 16:5294-303. 2005
    ..The reduced CP activity was restored by the coexpression of EhVps26, implying that the EhRab7A-mediated transport of CP to phagosomes is regulated by the retromerlike complex...
  38. ncbi request reprint Biochemical and functional characterization of phosphoserine aminotransferase from Entamoeba histolytica, which possesses both phosphorylated and non-phosphorylated serine metabolic pathways
    Vahab Ali
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371 8511, Japan
    Mol Biochem Parasitol 145:71-83. 2006
    ..Together with our previous demonstration of phosphoglycerate dehydrogenase, the present study reinforces physiological significance of the phosphorylated pathway in amoeba...
  39. ncbi request reprint New insights into molecular mechanisms of phagocytosis in Entamoeba histolytica by proteomic analysis
    Mami Okada
    Department of Parasitology, Gunma University Graduate School of Medicine, Showa machi, Maebashi, Gunma, Japan
    Arch Med Res 37:244-52. 2006
    ..These approaches should provide significant insights into molecular mechanisms of phagosome biogenesis and help to elucidate the pathogenesis of this important parasite...
  40. pmc Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area
    Ali Haghighi
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Shinjuku ku Tokyo Metropolitan Kiyose Children s Hospital, Kiyose shi, Tokyo, Japan
    J Clin Microbiol 40:4081-90. 2002
    ..We propose that genotyping of ameba isolates should help to determine geographic origins of isolates and routes of transmission...
  41. doi request reprint Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Cell Microbiol 11:1471-91. 2009
    ..Altogether, we demonstrated that PtdIns(3)P, PtdIns(4)P and EhFP4 coordinately regulate phagocytosis and phagosome maturation in this parasitic eukaryote...
  42. pmc Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica
    Fumika Mi-ichi
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    PLoS Negl Trop Dis 5:e1263. 2011
    ..The importance and topology of the components in E. histolytica mitosomes reinforce the notion that they are not "rudimentary" or "residual" mitochondria, but represent a uniquely evolved crucial organelle in E. histolytica...
  43. ncbi request reprint Rab5-associated vacuoles play a unique role in phagocytosis of the enteric protozoan parasite Entamoeba histolytica
    Yumiko Saito-Nakano
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 279:49497-507. 2004
    ....
  44. doi request reprint Analysis of autophagy in the enteric protozoan parasite Entamoeba
    Karina Picazarri
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi, Japan
    Methods Enzymol 451:359-71. 2008
    ..Here we describe the methods to study autophagy in Entamoeba...
  45. ncbi request reprint Diversity of clinical isolates of Entamoeba histolytica in Japan
    Tomoyoshi Nozaki
    Department of Parasitology, Gunma University Graduate School of Medicine, Showa machi, Maebashi, Gunma, Japan
    Arch Med Res 37:277-9. 2006
    ..We also review our current understanding of the diversity of Entamoeba histolytica clinical isolates in Japan, based on polymorphic genetic markers, clinical representations, and in vivo virulence, using an animal model...
  46. pmc Mitosomes in Entamoeba histolytica contain a sulfate activation pathway
    Fumika Mi-ichi
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Proc Natl Acad Sci U S A 106:21731-6. 2009
    ..These results suggest that sulfate activation is the major function of mitosomes in E. histolytica and that E. histolytica mitosomes represent a unique mitochondrion-related organelle with remarkable diversity...
  47. ncbi request reprint An intestinal parasitic protist, Entamoeba histolytica, possesses a non-redundant nitrogen fixation-like system for iron-sulfur cluster assembly under anaerobic conditions
    Vahab Ali
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162 8640
    J Biol Chem 279:16863-74. 2004
    ..This is the first demonstration of the presence and biological significance of the NIF-like system in eukaryotes...
  48. pmc Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens
    Karina Picazarri
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Infect Immun 76:278-88. 2008
    ..invadens. These results suggest that the core machinery for autophagy is conserved and plays an important role during proliferation and differentiation in Entamoeba...
  49. ncbi request reprint The diversity of Rab GTPases in Entamoeba histolytica
    Yumiko Saito-Nakano
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    Exp Parasitol 110:244-52. 2005
    ..We propose annotation of amebic Rab genes and discuss biological significance of this extraordinary diversity of EhRab proteins in this organism...
  50. doi request reprint Marked amplification and diversification of products of ras genes from rat brain, Rab GTPases, in the ciliates Tetrahymena thermophila and Paramecium tetraurelia
    Yumiko Saito-Nakano
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    J Eukaryot Microbiol 57:389-99. 2010
    ..These data suggested that the range of Rab is markedly amplified and diversified in ciliates, which may support the elaborate cellular structures and vigorous phagocytosis of those organisms...
  51. pmc Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica
    Mami Okada
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Eukaryot Cell 4:827-31. 2005
    ..Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis...
  52. pmc Expression, purification and crystallization of L-methionine gamma-lyase 2 from Entamoeba histolytica
    Dan Sato
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1034-6. 2006
    ..0 A. The presence of a tetramer in the asymmetric unit (4 x 43.1 kDa) gives a Matthews coefficient of 2.2 A(3) Da(-1). The structure was solved by the molecular-replacement method and structure refinement is now in progress...
  53. ncbi request reprint Characterisation of hexokinase in Toxoplasma gondii tachyzoites
    Tomoya Saito
    Department of Tropical Medicine and Parasitology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Int J Parasitol 32:961-7. 2002
    ..gondii tachyzoites. This result suggests that T. gondii hexokinase weakly associates with the membrane or particulate fraction of the tachyzoite cell...
  54. pmc Amebiasis in HIV-1-infected Japanese men: clinical features and response to therapy
    Koji Watanabe
    AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan
    PLoS Negl Trop Dis 5:e1318. 2011
    ..Luminal treatment following metronidazole or tinidazole treatment does not reduce recurrence of amebiasis in high risk populations probably due to amebic re-infection...
  55. pmc A case of quadruple malaria infection imported from mozambique to Japan
    Masayuki Oki
    Department of Internal Medicine, Department of Laboratory Medicine, and Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa, Japan Clinical Laboratory Center, Tokai University Hospital, Isehara, Kanagawa, Japan Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    Am J Trop Med Hyg 90:1098-101. 2014
    ..All four species must be identified by using sensitive and specific tests, such as a nested polymerase chain reaction, in addition to conventional morphologic identification. ..
  56. doi request reprint Highly divergent mitochondrion-related organelles in anaerobic parasitic protozoa
    Takashi Makiuchi
    Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259 1193, Japan Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biochimie 100:3-17. 2014
    ..In this review, we summarize and discuss the diverse metabolic functions and protein transport systems of the MROs from anaerobic parasitic protozoa. ..
  57. ncbi request reprint Expression profiles of peroxiredoxin proteins of the rodent malaria parasite Plasmodium yoelii
    Shin ichiro Kawazu
    Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku ku, 162 8655, Tokyo, Japan
    Int J Parasitol 33:1455-61. 2003
    ....
  58. pmc Crystallization and preliminary X-ray analysis of L-methionine gamma-lyase 1 from Entamoeba histolytica
    Dan Sato
    Institute for Advanced Biosciences, Keio University, 246 2 Mizukami, Kakuganji, Tsuruoka, Yamagata 997 0052, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:697-9. 2008
    ..4 kDa) gives a Matthews coefficient of 2.8 A(3) Da(-1) and a solvent content of 56%. The structure was solved by the molecular-replacement method and structure refinement is now in progress...
  59. ncbi request reprint [Attention-getting zoonoses: Cryptosporidiosis]
    Tomoyoshi Nozaki
    Nihon Naika Gakkai Zasshi 96:2413-7. 2007
  60. ncbi request reprint Identification and characterization of two isoenzymes of methionine gamma-lyase from Entamoeba histolytica: a key enzyme of sulfur-amino acid degradation in an anaerobic parasitic protist that lacks forward and reverse trans-sulfuration pathways
    Masaharu Tokoro
    Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 278:42717-27. 2003
    ....