Affiliation: National Research Institute for Child Health and Development
- Escherichia coli Shiga toxinH Nakao
Department of Infectious Diseases Research, National Children s Medical Research Center, National Children s Hospital, Tokyo, Japan
J Nat Toxins 9:299-313. 2000..Apoptosis is considered to contribute to the pathogenesis of HUS caused by STEC. In this review, recent progress in Stx-related research is summarized...
- Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicityHiroshi Nakao
Department of Infectious Diseases, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo 154 8567, Japan
Microbiol Immunol 46:777-80. 2002....
- Subtyping of Shiga toxin 2 variants in human-derived Shiga toxin-producing Escherichia coli strains isolated in JapanHiroshi Nakao
Department of Infectious Diseases, National Research Institute for Child Health and Development, 3 35 31 Taishido, Setagaya Ku, Tokyo 154 8567Japan
FEMS Immunol Med Microbiol 34:289-97. 2002..A multiplex PCR that can detect the stx1, stx2, and G1 genes was developed as a means of rapid and easy typing to better understand the roles of the different types of Stx...
- Characterization of a shiga-toxin 1-resistant stock of vero cellsTakaomi Sekino
Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
Microbiol Immunol 48:377-87. 2004..Further study of R-Vero cells may provide a model of Stx1 resistance via distinct intracellular transport of Stx1...
- Inhibition of shiga toxin cytotoxicity in human renal cortical epithelial cells by nitrobenzylthioinosineTakaomi Sekino
Department of Pathology, National Children s Medical Research Center, Setagaya Ku, Tokyo, Japan
J Infect Dis 185:785-96. 2002..Investigation of the NBTI-mediated protection mechanism against Stx cytotoxicity may provide insights into the analysis of Stx-mediated cell damage and lead to improvements in therapeutic approaches for diseases caused by Stx...
- [Shiga toxin producing Escherichia coli infection]Hiroshi Nakao
Department of Infectious Diseases Research, National Children s Medical Research Center, National Children s Hospital
Nihon Rinsho 60:545-50. 2002..In conclusion, the prevention of primary infection is thought to be the best way to prevent the life-threatening complications caused by STEC, and second way is identification as early as possible...
- Mixture of sugar and povidone-iodine stimulates healing of MRSA-infected skin ulcers on db/db miceChong Ming Shi
Department of Dermatology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
Arch Dermatol Res 299:449-56. 2007..These results indicate that wounding on db/db mice provides a useful animal model of bacterial skin infections, and that SP is an effective topical agent for the treatment of diabetic skin ulcers...
- Development of humanized monoclonal antibody TMA-15 which neutralizes Shiga toxin 2Tsuyoshi Kimura
Teijin Institute for Biomedical Research, Teijin Limited, 4 3 2 Asahigaoka, Hino, Tokyo 191 8512, Japan
Hybrid Hybridomics 21:161-8. 2002..These results suggest that TMA-15 will have clinical potency in Stx-producing Escherichia coli infections, including E. coli O157 infections...
- Characterization of a Shiga toxin 1-neutralizing recombinant Fab fragment isolated by phage display systemKazuyuki Inoue
Department of Pharmaceutical Science, Akita University Hospital, Hondo, Japan
Tohoku J Exp Med 203:295-303. 2004..Among the regions, CDR2 showed the most frequent nucleotide and amino acid substitutions. These results suggest that heavy chain CDR2 may mainly be associated with the 5-5B function, that is neutralizing cytotoxicity of Stx1...
- Identification and characterization of two contiguous operons required for aerobactin transport and biosynthesis in Vibrio mimicusYong Hwa Moon
Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan
Microbiol Immunol 48:389-98. 2004..This is the first report demonstrating that aerobactin transport and biosynthesis genes are present in a species outside the family Enterobacteriaceae...
- Identification of an AraC-like regulator gene required for induction of the 78-kDa ferrioxamine B receptor in Vibrio vulnificusTomotaka Tanabe
Faculty of Pharmaceutical Sciences, Okayama University, 1 1 1 Tsushima naka, Okayama 700 8530, Japan
FEMS Microbiol Lett 249:309-14. 2005..These results suggest that the desR gene is required for desferrioxamine B-inducible production of DesA in iron-starved cells...
- A novel aerobactin utilization cluster in Vibrio vulnificus with a gene involved in the transcription regulation of the iutA homologueTomotaka Tanabe
Department of Molecular Biopharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 1 1 1 Tsushima naka, Okayama, Japan
Microbiol Immunol 49:823-34. 2005..This is the first example of a regulator gene involved in aerobactin-enhanced production of IutA...
- Involvement of the Vibrio parahaemolyticus pvsC gene in export of the siderophore vibrioferrinTomotaka Tanabe
Department of Molecular Biopharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
Microbiol Immunol 50:871-6. 2006..Vibrioferrin release could be regained by introducing the intact pvsC gene on a complementing plasmid. These results indicate that the pvsC gene product functions as an inner membrane exporter of vibrioferrin...
- An iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in Vibrio parahaemolyticusTatsuya Funahashi
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
Microbiology 149:1217-25. 2003..parahaemolyticus. However, additional genes required for ferric aerobactin transport across the inner membrane remain to be clarified...
- Variation of extracellular proteases produced by Vibrio vulnificus clinical isolates: genetic diversity of the metalloprotease gene (vvp), and serine protease secretion by vvp-negative strainsJiyou Wang
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
Microb Pathog 44:494-500. 2008..These findings suggest that, in addition to metalloprotease, the extracellular serine protease may contribute to pathogenicity of V. vulnificus...