Affiliation: National Institute for Materials Science
- Spatiotemporal control of migration of single cells on a photoactivatable cell microarrayJun Nakanishi
Bioengineering Laboratory, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
J Am Chem Soc 129:6694-5. 2007
- [Control of cellular microenvironment with a caged compound immobilized on a surface]Jun Nakanishi
Tanpakushitsu Kakusan Koso 52:1613-8. 2007
- Recent advances in cell micropatterning techniques for bioanalytical and biomedical sciencesJun Nakanishi
International Center for Young Scientists, National Institute for Materials Science, 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
Anal Sci 24:67-72. 2008..As an example of dynamic substrates, we introduce a dynamic substrate based on a caged compound, where we accomplished a light-driven alteration of cell adhesiveness and the analysis of a single cell's motility...
- Light-regulated activation of cellular signaling by gold nanoparticles that capture and release aminesJun Nakanishi
World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics, National Institute for Materials Science NIMS, 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
J Am Chem Soc 131:3822-3. 2009..The present work will allow for new possibilities in studies of inter- and intracellular signaling networks...
- Arraying heterotypic single cells on photoactivatable cell-culturing substratesYukiko Kikuchi
World Premier International WPI Research Center Initiative, International Center for Materials Nanoarchitectonics MANA and National Institute for Materials Sciences NIMS, Tsukuba, Japan
Langmuir 24:13084-95. 2008..The present method will be particularly useful in studying the dynamic processes of cell-cell interactions at a single-cell level...
- Silane coupling agent bearing a photoremovable succinimidyl carbonate for patterning amines on glass and silicon surfaces with controlled surface densitiesHidekazu Nakayama
World Premier International WPI Research Center Initiative, International Center for Materials Nanoarchitectonics MANA and National Institute for Materials Science NIMS, Namiki, Tsukuba, Japan
Colloids Surf B Biointerfaces 76:88-97. 2010..Furthermore, we also succeeded in forming a chemical density gradient at a lower surface density range (0.15-0.011 chains/nm(2)) on the substrate by diluting the feed amino-biotin with an inert control amine...
- Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linkerShingo Kaneko
World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics MANA and National Institute for Materials Science, 1 1 Namiki, Tsukuba 305 0044, Japan
Phys Chem Chem Phys 13:4051-9. 2011..We believe this method will be useful for dynamic patterning of cells on protein-based scaffolds...
- Photoactivation of a substrate for cell adhesion under standard fluorescence microscopesJun Nakanishi
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
J Am Chem Soc 126:16314-5. 2004..The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions...
- FRET-based monitoring of conformational change of the beta2 adrenergic receptor in living cellsJun Nakanishi
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan
Biochem Biophys Res Commun 343:1191-6. 2006..It was suggested that this discrepancy corresponds directly to the diametric biological functions, i.e., the activation or inactivation of adenylyl cyclase...
- Display of functionally active PHB depolymerase on Escherichia coli cell surfaceTomohiro Hiraishi
Bioengineering Laboratory, RIKEN Advanced Science Institute, Wako Shi, Saitama, Japan
Macromol Biosci 12:218-24. 2012..The results demonstrate that PhaZ(RpiT1) -displaying E. coli shows potential for use as a whole-cell biocatalyst for the production of (R)-3-hydroxybutyrate monomers from insoluble PHB materials...