Research Topics
Genomes and Genes | Akira NagasakiSummaryAffiliation: National Institute of Advanced Industrial Science and Technology Country: Japan Publications
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Detail Information
Publications
A novel shRNA vector that enables rapid selection and identification of knockdown cellsAkira Nagasaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
Plasmid 58:190-4. 2007..It thus should be possible to analyze the biochemical consequences of gene silencing en masse in the absence of a background of untransfected cells...
Screening of genes involved in cell migration in DictyosteliumAkira Nagasaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki, Japan
Exp Cell Res 314:1136-46. 2008..These results suggest that PLDB plays an important role in migration in Dictyostelium cells, and that our screening system is useful for the identification of genes involved in cell migration...- Phospholipase D is essential for keratocyte-like migration of NBT-II cellsAkira Nagasaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
Cell Struct Funct 33:27-33. 2008..Furthermore, GFP-PLD2 was localized to the protruding regions of lamellipodia in migrating cells. Thus, PLD is essential for the maintenance of keratocyte-like locomotion of NBT-II cells, presumably by regulating the actin cytoskeleton... - Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideumAkira Nagasaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8562, Japan
Cell Res 19:236-46. 2009..Furthermore, that paxB(-) cells fail to divide normally in the presence of substrate adhesion suggests that this adhesion molecule may play additional signaling roles... 
DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in DictyosteliumAkira Nagasaki
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
Mol Biol Cell 15:435-46. 2004....- Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic dictyostelium cellsAkira Nagasaki
Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Ibaraki 305 8562, Japan
Mol Biol Cell 13:4333-42. 2002..Taken together, these findings suggest MHCK C drives the disassembly of myosin II filaments for efficient cytokinesis and recycling of myosin II that occurs during cytokinesis... - Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migrationReiko Onuki-Nagasaki
Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Tokyo, Japan
Methods Mol Biol 629:193-203. 2010..To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening... - Novel functions of Ect2 in polar lamellipodia formation and polarity maintenance during "contractile ring-independent" cytokinesis in adherent cellsMasamitsu Kanada
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
Mol Biol Cell 19:8-16. 2008..These results suggest that equatorial Ect2 locally suppresses lamellipodia formation via RhoA activation, which indirectly contributes to restricting lamellipodia formation to polar regions during cytokinesis B... - Stabilization of anaphase midzone microtubules is regulated by Rho during cytokinesis in human fibrosarcoma cellsMasamitsu Kanada
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
Exp Cell Res 315:2705-14. 2009..Natl. Acad. Sci. U. S. A. 102 (2005) 9529-9534), suggest that mammalian cells have two potential steps that require active Rho for the stabilization of midzone MTs during mitosis and cytokinesis... - Chemotaxis-mediated scission contributes to efficient cytokinesis in DictyosteliumAkira Nagasaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
Cell Motil Cytoskeleton 65:896-903. 2008..8% when they were surrounded by Gbeta-null cells. Thus, Gbeta-null cells frequently fail to divide because they cannot assist each other's division and midwifery ensures successful cytokinesis in Dictyostelium discoideum... - Stretching actin filaments within cells enhances their affinity for the myosin II motor domainTaro Q P Uyeda
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
PLoS ONE 6:e26200. 2011.... - Correlated waves of actin filaments and PIP3 in Dictyostelium cellsYukako Asano
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
Cell Motil Cytoskeleton 65:923-34. 2008..We propose that the waves of PIP(3) and actin filaments are loosely coupled with each other and play important roles in generating spontaneous cell polarity... - Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in DictyosteliumAkira Nagasaki
Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology (AIST, Tsukuba, Ibaraki 305-8562, Japan
J Cell Sci 115:2241-51. 2002..We take this as genetic evidence for two parallel pathways both of which lead to cell-cycle-coupled cytokinesis. This conclusion is supported by differences in morphological changes during cytokinesis in the mutant cell lines... - Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivoNobuhisa Umeki
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
J Biol Chem 288:1739-49. 2013..We have recently found that two other dominant negative actin mutants are also defective in cofilin binding, and we propose that the defective cofilin binder is a major class of dominant negative actin mutants... - Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activationReiko Onuki
Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan
Proc Natl Acad Sci U S A 99:14716-21. 2002.... - On-chip screening method for cell migration genes based on a transfection microarrayReiko Onuki-Nagasaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tokyo, 135 0064, Japan
Lab Chip 8:1502-6. 2008..Our new method will lead to an enhanced understanding of cell migration... - Overlapping functions of the two talin homologues in DictyosteliumMasatsune Tsujioka
National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
Eukaryot Cell 7:906-16. 2008..This is the first study demonstrating overlapping functions of two talin homologues, and our data further indicate the importance of talin... - Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cellsMasamitsu Kanada
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
Mol Biol Cell 16:3865-72. 2005..These results provide direct evidence for adhesion-dependent, contractile ring-independent equatorial furrowing in mammalian cells and demonstrate the importance of substrate adhesion for cytokinesis... - Multiple parallelisms in animal cytokinesisTaro Q P Uyeda
Gene Function Research Center, National Institute for Advanced Industrial Science and Technology (AIST, Tsukuba, Japan
Int Rev Cytol 240:377-432. 2004.... - Keratocyte-like locomotion in amiB-null Dictyostelium cellsYukako Asano
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
Cell Motil Cytoskeleton 59:17-27. 2004..This mutant cell can be a useful tool for further molecular genetic analysis of the mechanism of cell locomotion... - Variations on a theme: the many modes of cytokinesisTaro Q P Uyeda
Gene Function Research Center, Tsukuba Central 4, AIST, Tsukuba, Ibaraki 305 8562, Japan
Curr Opin Cell Biol 16:55-60. 2004..An additional chemotaxis-based cytokinesis that involves a 'midwife' cell has also been reported. Collectively, these findings demonstrate an unexpected diversity of mechanisms by which animal cells carry out cytokinesis... - Desalted deep sea water increases transformation and homologous recombination efficiencies in Dictyostelium discoideumHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
J Mol Microbiol Biotechnol 14:157-62. 2008..These results indicate that a simple modification of the solvent medium remarkably enhanced the isolation of transformants and gene-targeted clones, which had previously been difficult to isolate... - Dictyostelium discoideum talin A is crucial for myosin II-independent and adhesion-dependent cytokinesisMakoto Hibi
Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan
J Muscle Res Cell Motil 25:127-40. 2004.... - Multiple myosin II heavy chain kinases: roles in filament assembly control and proper cytokinesis in DictyosteliumShigehiko Yumura
Department of Biology, Faculty of Science, Yamaguchi University, Yamaguchi 753 8512, Japan
Mol Biol Cell 16:4256-66. 2005..These studies suggest that the full complement of MHC kinases that significantly contribute to growth phase and cytokinesis myosin II disassembly in this organism has now been identified...