Akira Nagasaki

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. ncbi request reprint A novel shRNA vector that enables rapid selection and identification of knockdown cells
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
    Plasmid 58:190-4. 2007
  2. ncbi request reprint Phospholipase D is essential for keratocyte-like migration of NBT-II cells
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
    Cell Struct Funct 33:27-33. 2008
  3. doi request reprint Screening of genes involved in cell migration in Dictyostelium
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki, Japan
    Exp Cell Res 314:1136-46. 2008
  4. doi request reprint Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8562, Japan
    Cell Res 19:236-46. 2009
  5. pmc DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium
    Akira Nagasaki
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Mol Biol Cell 15:435-46. 2004
  6. pmc Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic dictyostelium cells
    Akira Nagasaki
    Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Ibaraki 305 8562, Japan
    Mol Biol Cell 13:4333-42. 2002
  7. doi request reprint Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration
    Reiko Onuki-Nagasaki
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Tokyo, Japan
    Methods Mol Biol 629:193-203. 2010
  8. pmc Novel functions of Ect2 in polar lamellipodia formation and polarity maintenance during "contractile ring-independent" cytokinesis in adherent cells
    Masamitsu Kanada
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mol Biol Cell 19:8-16. 2008
  9. doi request reprint Stabilization of anaphase midzone microtubules is regulated by Rho during cytokinesis in human fibrosarcoma cells
    Masamitsu Kanada
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Exp Cell Res 315:2705-14. 2009
  10. doi request reprint Chemotaxis-mediated scission contributes to efficient cytokinesis in Dictyostelium
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Cell Motil Cytoskeleton 65:896-903. 2008

Collaborators

Detail Information

Publications24

  1. ncbi request reprint A novel shRNA vector that enables rapid selection and identification of knockdown cells
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
    Plasmid 58:190-4. 2007
    ..It thus should be possible to analyze the biochemical consequences of gene silencing en masse in the absence of a background of untransfected cells...
  2. ncbi request reprint Phospholipase D is essential for keratocyte-like migration of NBT-II cells
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
    Cell Struct Funct 33:27-33. 2008
    ..Furthermore, GFP-PLD2 was localized to the protruding regions of lamellipodia in migrating cells. Thus, PLD is essential for the maintenance of keratocyte-like locomotion of NBT-II cells, presumably by regulating the actin cytoskeleton...
  3. doi request reprint Screening of genes involved in cell migration in Dictyostelium
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki, Japan
    Exp Cell Res 314:1136-46. 2008
    ..These results suggest that PLDB plays an important role in migration in Dictyostelium cells, and that our screening system is useful for the identification of genes involved in cell migration...
  4. doi request reprint Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8562, Japan
    Cell Res 19:236-46. 2009
    ..Furthermore, that paxB(-) cells fail to divide normally in the presence of substrate adhesion suggests that this adhesion molecule may play additional signaling roles...
  5. pmc DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium
    Akira Nagasaki
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Mol Biol Cell 15:435-46. 2004
    ....
  6. pmc Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic dictyostelium cells
    Akira Nagasaki
    Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Ibaraki 305 8562, Japan
    Mol Biol Cell 13:4333-42. 2002
    ..Taken together, these findings suggest MHCK C drives the disassembly of myosin II filaments for efficient cytokinesis and recycling of myosin II that occurs during cytokinesis...
  7. doi request reprint Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration
    Reiko Onuki-Nagasaki
    Research Institute for Cell Engineering RICE, National Institute of Advanced Industrial Science and Technology AIST, Tokyo, Japan
    Methods Mol Biol 629:193-203. 2010
    ..To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening...
  8. pmc Novel functions of Ect2 in polar lamellipodia formation and polarity maintenance during "contractile ring-independent" cytokinesis in adherent cells
    Masamitsu Kanada
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mol Biol Cell 19:8-16. 2008
    ..These results suggest that equatorial Ect2 locally suppresses lamellipodia formation via RhoA activation, which indirectly contributes to restricting lamellipodia formation to polar regions during cytokinesis B...
  9. doi request reprint Stabilization of anaphase midzone microtubules is regulated by Rho during cytokinesis in human fibrosarcoma cells
    Masamitsu Kanada
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Exp Cell Res 315:2705-14. 2009
    ..Natl. Acad. Sci. U. S. A. 102 (2005) 9529-9534), suggest that mammalian cells have two potential steps that require active Rho for the stabilization of midzone MTs during mitosis and cytokinesis...
  10. doi request reprint Chemotaxis-mediated scission contributes to efficient cytokinesis in Dictyostelium
    Akira Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Cell Motil Cytoskeleton 65:896-903. 2008
    ..8% when they were surrounded by Gbeta-null cells. Thus, Gbeta-null cells frequently fail to divide because they cannot assist each other's division and midwifery ensures successful cytokinesis in Dictyostelium discoideum...
  11. pmc Stretching actin filaments within cells enhances their affinity for the myosin II motor domain
    Taro Q P Uyeda
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    PLoS ONE 6:e26200. 2011
    ....
  12. doi request reprint Correlated waves of actin filaments and PIP3 in Dictyostelium cells
    Yukako Asano
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Cell Motil Cytoskeleton 65:923-34. 2008
    ..We propose that the waves of PIP(3) and actin filaments are loosely coupled with each other and play important roles in generating spontaneous cell polarity...
  13. ncbi request reprint Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in Dictyostelium
    Akira Nagasaki
    Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8562, Japan
    J Cell Sci 115:2241-51. 2002
    ..We take this as genetic evidence for two parallel pathways both of which lead to cell-cycle-coupled cytokinesis. This conclusion is supported by differences in morphological changes during cytokinesis in the mutant cell lines...
  14. pmc Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo
    Nobuhisa Umeki
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    J Biol Chem 288:1739-49. 2013
    ..We have recently found that two other dominant negative actin mutants are also defective in cofilin binding, and we propose that the defective cofilin binder is a major class of dominant negative actin mutants...
  15. pmc Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation
    Reiko Onuki
    Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    Proc Natl Acad Sci U S A 99:14716-21. 2002
    ....
  16. doi request reprint On-chip screening method for cell migration genes based on a transfection microarray
    Reiko Onuki-Nagasaki
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tokyo, 135 0064, Japan
    Lab Chip 8:1502-6. 2008
    ..Our new method will lead to an enhanced understanding of cell migration...
  17. pmc Overlapping functions of the two talin homologues in Dictyostelium
    Masatsune Tsujioka
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Eukaryot Cell 7:906-16. 2008
    ..This is the first study demonstrating overlapping functions of two talin homologues, and our data further indicate the importance of talin...
  18. pmc Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells
    Masamitsu Kanada
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mol Biol Cell 16:3865-72. 2005
    ..These results provide direct evidence for adhesion-dependent, contractile ring-independent equatorial furrowing in mammalian cells and demonstrate the importance of substrate adhesion for cytokinesis...
  19. ncbi request reprint Multiple parallelisms in animal cytokinesis
    Taro Q P Uyeda
    Gene Function Research Center, National Institute for Advanced Industrial Science and Technology AIST, Tsukuba, Japan
    Int Rev Cytol 240:377-432. 2004
    ....
  20. ncbi request reprint Keratocyte-like locomotion in amiB-null Dictyostelium cells
    Yukako Asano
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Cell Motil Cytoskeleton 59:17-27. 2004
    ..This mutant cell can be a useful tool for further molecular genetic analysis of the mechanism of cell locomotion...
  21. ncbi request reprint Variations on a theme: the many modes of cytokinesis
    Taro Q P Uyeda
    Gene Function Research Center, Tsukuba Central 4, AIST, Tsukuba, Ibaraki 305 8562, Japan
    Curr Opin Cell Biol 16:55-60. 2004
    ..An additional chemotaxis-based cytokinesis that involves a 'midwife' cell has also been reported. Collectively, these findings demonstrate an unexpected diversity of mechanisms by which animal cells carry out cytokinesis...
  22. ncbi request reprint Desalted deep sea water increases transformation and homologous recombination efficiencies in Dictyostelium discoideum
    Hidekazu Kuwayama
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
    J Mol Microbiol Biotechnol 14:157-62. 2008
    ..These results indicate that a simple modification of the solvent medium remarkably enhanced the isolation of transformants and gene-targeted clones, which had previously been difficult to isolate...
  23. ncbi request reprint Dictyostelium discoideum talin A is crucial for myosin II-independent and adhesion-dependent cytokinesis
    Makoto Hibi
    Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060 0810, Japan
    J Muscle Res Cell Motil 25:127-40. 2004
    ....
  24. pmc Multiple myosin II heavy chain kinases: roles in filament assembly control and proper cytokinesis in Dictyostelium
    Shigehiko Yumura
    Department of Biology, Faculty of Science, Yamaguchi University, Yamaguchi 753 8512, Japan
    Mol Biol Cell 16:4256-66. 2005
    ..These studies suggest that the full complement of MHC kinases that significantly contribute to growth phase and cytokinesis myosin II disassembly in this organism has now been identified...