Research Topics
| Yuichi MurayamaSummaryAffiliation: National Institute of Agrobiological Sciences Country: Japan Publications
| Collaborators
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Detail Information
Publications
Ultrasensitive detection of scrapie prion protein derived from ARQ and AHQ homozygote sheep by interspecies in vitro amplificationYuichi Murayama
Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
Microbiol Immunol 56:541-7. 2012..The interspecies PMCA developed in this study may be useful in the detailed analysis of PrP(Sc) distribution in classical scrapie-infected ARQ and AHQ homozygote sheep...
Urinary excretion and blood level of prions in scrapie-infected hamstersYuichi Murayama
Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
J Gen Virol 88:2890-8. 2007..These results represent the first biochemical detection of PrP(Sc) in urine from TSE-infected animals...- Specific detection of prion antigenic determinants retained in bovine meat and bone meal by flow microbead immunoassayY Murayama
Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
J Appl Microbiol 101:369-76. 2006.... - Protein misfolding cyclic amplification as a rapid test for assessment of prion inactivationYuichi Murayama
Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
Biochem Biophys Res Commun 348:758-62. 2006..Three rounds of PMCA require only 6 days, which means that the PMCA method is much faster than the bioassay... - Efficient in vitro amplification of a mouse-adapted scrapie prion proteinYuichi Murayama
Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
Neurosci Lett 413:270-3. 2007..The present study is the first report describing effective amplification of PrP(Sc) of the mouse-adapted scrapie prion and this improved PMCA technique will contribute to prion research that uses mice as experimental animals... 
Intraspecies prion transmission results in selection of sheep scrapie strainsTakashi Yokoyama
Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Japan
PLoS ONE 5:e15450. 2010..The heterogeneity of natural scrapie prions in individual sheep and in sheep flocks has not been clearly defined...- Anti-prion activity of Brilliant Blue GYoshifumi Iwamaru
Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
PLoS ONE 7:e37896. 2012.... - Sc237 hamster PrPSc and Sc237-derived mouse PrPSc generated by interspecies in vitro amplification exhibit distinct pathological and biochemical properties in tga20 transgenic miceMiyako Yoshioka
Safety Research Team, Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
Microbiol Immunol 55:331-40. 2011..In addition, these PrP(Sc) exhibited distinctive virulence profiles upon secondary passage. These results suggest that different in vivo and in vitro environments result in propagation of PrP(Sc) with different biological properties... - Glycosylphosphatidylinositol anchor-dependent stimulation pathway required for generation of baculovirus-derived recombinant scrapie prion proteinMorikazu Imamura
Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
J Virol 85:2582-8. 2011..Therefore, the Bac-PMCA technique appears to be extremely beneficial for the comprehensive understanding of the GPI anchor-mediated stimulation pathway... - Lactoferrin induces cell surface retention of prion protein and inhibits prion accumulationYoshifumi Iwamaru
Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
J Neurochem 107:636-46. 2008..Furthermore, LF partially inhibited the formation of protease-resistant PrP as determined by the protein misfolding cyclic amplification assay. Our results suggest that LF has multifunctional antiprion activities... - Accumulation of L-type bovine prions in peripheral nerve tissuesYoshifumi Iwamaru
National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
Emerg Infect Dis 16:1151-4. 2010..As with classical BSE prions, L-type BSE prions accumulated in central and peripheral nerve tissues... - Sensitive detection of scrapie prion protein in soilKazunari Nagaoka
Soil Microbiology Research Team, National Agricultural Research Center, 3 1 1 Kannondai, Tsukuba, Ibaraki 305 8666, Japan
Biochem Biophys Res Commun 397:626-30. 2010..Our method appears to be a very useful technique for monitoring PrP(Sc) levels in soil... - Experimental transmission of two young and one suspended bovine spongiform encephalopathy (BSE) cases to bovinized transgenic miceTakashi Yokoyama
Prion Disease Research Center, National Institute of Animal Health, Ibaraki 305 0856, Japan
Jpn J Infect Dis 60:317-20. 2007..The results of a dilution experiment using the classical BSE prion indicated that prion infectivity in these 3 cattle was below the detection limit of 10(3.0) LD(50)/g... - Examination of the offspring of a Japanese cow affected with L-type bovine spongiform encephalopathyTakashi Yokoyama
Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
J Vet Med Sci 73:121-3. 2011..Transmission of L-BSE was not observed during 4 years of observation, though the steer was born when the dam was in the terminal stages of the disease... - Assessment of prion inactivation by combined use of Bacillus-derived protease and SDSMiyako Yoshioka
Prion Disease Research Center, National Institute of Animal Health, Japan
Biosci Biotechnol Biochem 71:2565-8. 2007..The treatment therefore can be applied to the decontamination of thermolabile instruments... - Fusarenon-X induced apoptosis in HL-60 cells depends on caspase activation and cytochrome c releaseKatsuhiro Miura
Department of Safety Research, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, 305, Ibaraki, Japan
Toxicology 172:103-12. 2002..These findings suggest that FX induces apoptosis in HL-60 cells by stimulating cytochrome c release followed by its downstream events including the activation of multiple caspases... - Characterization of a proteolytic enzyme derived from a Bacillus strain that effectively degrades prion proteinM Yoshioka
National Institute of Animal Health, Tsukuba, Ibaraki, Japan
J Appl Microbiol 102:509-15. 2007..This paper describes the biochemical characteristics and proteolytic activity of the isolated protease... - Assessment of prion inactivation by fenton reaction using protein misfolding cyclic amplification and bioassayKyozo Suyama
Sports Nutrition Department, Sendai University, Funaoka Minami, Shibata, Japan
Biosci Biotechnol Biochem 71:2069-71. 2007..Therefore, the Fenton reaction has potential for prion decontamination... - Prion inactivation by the Maillard reactionKyozo Suyama
Sports Nutrition Department, Sendai University, Miyagi, Japan
Biochem Biophys Res Commun 356:245-8. 2007..Therefore, the Maillard reaction can be employed for the decontamination of large amounts of byproducts such as MBM...