Yuichi Murayama

Summary

Affiliation: National Institute of Agrobiological Sciences
Country: Japan

Publications

  1. pmc Insect cell-derived cofactors become fully functional after proteinase K and heat treatment for high-fidelity amplification of glycosylphosphatidylinositol-anchored recombinant scrapie and BSE prion proteins
    Morikazu Imamura
    Influenza Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    PLoS ONE 8:e82538. 2013
  2. doi request reprint Ultrasensitive detection of scrapie prion protein derived from ARQ and AHQ homozygote sheep by interspecies in vitro amplification
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    Microbiol Immunol 56:541-7. 2012
  3. ncbi request reprint Efficient in vitro amplification of a mouse-adapted scrapie prion protein
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    Neurosci Lett 413:270-3. 2007
  4. ncbi request reprint Urinary excretion and blood level of prions in scrapie-infected hamsters
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    J Gen Virol 88:2890-8. 2007
  5. ncbi request reprint Specific detection of prion antigenic determinants retained in bovine meat and bone meal by flow microbead immunoassay
    Y Murayama
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
    J Appl Microbiol 101:369-76. 2006
  6. ncbi request reprint Protein misfolding cyclic amplification as a rapid test for assessment of prion inactivation
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
    Biochem Biophys Res Commun 348:758-62. 2006
  7. pmc Intraspecies prion transmission results in selection of sheep scrapie strains
    Takashi Yokoyama
    Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Japan
    PLoS ONE 5:e15450. 2010
  8. pmc Anti-prion activity of Brilliant Blue G
    Yoshifumi Iwamaru
    Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    PLoS ONE 7:e37896. 2012
  9. doi request reprint Sc237 hamster PrPSc and Sc237-derived mouse PrPSc generated by interspecies in vitro amplification exhibit distinct pathological and biochemical properties in tga20 transgenic mice
    Miyako Yoshioka
    Safety Research Team, Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    Microbiol Immunol 55:331-40. 2011
  10. pmc Glycosylphosphatidylinositol anchor-dependent stimulation pathway required for generation of baculovirus-derived recombinant scrapie prion protein
    Morikazu Imamura
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    J Virol 85:2582-8. 2011

Collaborators

Detail Information

Publications24

  1. pmc Insect cell-derived cofactors become fully functional after proteinase K and heat treatment for high-fidelity amplification of glycosylphosphatidylinositol-anchored recombinant scrapie and BSE prion proteins
    Morikazu Imamura
    Influenza Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    PLoS ONE 8:e82538. 2013
    ..PMCA using only insect cell-derived substances (iPMCA) was highly useful for the ultrasensitive detection of PrP(Sc) of some prion strains. ..
  2. doi request reprint Ultrasensitive detection of scrapie prion protein derived from ARQ and AHQ homozygote sheep by interspecies in vitro amplification
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    Microbiol Immunol 56:541-7. 2012
    ..The interspecies PMCA developed in this study may be useful in the detailed analysis of PrP(Sc) distribution in classical scrapie-infected ARQ and AHQ homozygote sheep...
  3. ncbi request reprint Efficient in vitro amplification of a mouse-adapted scrapie prion protein
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    Neurosci Lett 413:270-3. 2007
    ..The present study is the first report describing effective amplification of PrP(Sc) of the mouse-adapted scrapie prion and this improved PMCA technique will contribute to prion research that uses mice as experimental animals...
  4. ncbi request reprint Urinary excretion and blood level of prions in scrapie-infected hamsters
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    J Gen Virol 88:2890-8. 2007
    ..These results represent the first biochemical detection of PrP(Sc) in urine from TSE-infected animals...
  5. ncbi request reprint Specific detection of prion antigenic determinants retained in bovine meat and bone meal by flow microbead immunoassay
    Y Murayama
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
    J Appl Microbiol 101:369-76. 2006
    ....
  6. ncbi request reprint Protein misfolding cyclic amplification as a rapid test for assessment of prion inactivation
    Yuichi Murayama
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
    Biochem Biophys Res Commun 348:758-62. 2006
    ..Three rounds of PMCA require only 6 days, which means that the PMCA method is much faster than the bioassay...
  7. pmc Intraspecies prion transmission results in selection of sheep scrapie strains
    Takashi Yokoyama
    Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Japan
    PLoS ONE 5:e15450. 2010
    ..The heterogeneity of natural scrapie prions in individual sheep and in sheep flocks has not been clearly defined...
  8. pmc Anti-prion activity of Brilliant Blue G
    Yoshifumi Iwamaru
    Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    PLoS ONE 7:e37896. 2012
    ....
  9. doi request reprint Sc237 hamster PrPSc and Sc237-derived mouse PrPSc generated by interspecies in vitro amplification exhibit distinct pathological and biochemical properties in tga20 transgenic mice
    Miyako Yoshioka
    Safety Research Team, Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    Microbiol Immunol 55:331-40. 2011
    ..In addition, these PrP(Sc) exhibited distinctive virulence profiles upon secondary passage. These results suggest that different in vivo and in vitro environments result in propagation of PrP(Sc) with different biological properties...
  10. pmc Glycosylphosphatidylinositol anchor-dependent stimulation pathway required for generation of baculovirus-derived recombinant scrapie prion protein
    Morikazu Imamura
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    J Virol 85:2582-8. 2011
    ..Therefore, the Bac-PMCA technique appears to be extremely beneficial for the comprehensive understanding of the GPI anchor-mediated stimulation pathway...
  11. doi request reprint Lactoferrin induces cell surface retention of prion protein and inhibits prion accumulation
    Yoshifumi Iwamaru
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
    J Neurochem 107:636-46. 2008
    ..Furthermore, LF partially inhibited the formation of protease-resistant PrP as determined by the protein misfolding cyclic amplification assay. Our results suggest that LF has multifunctional antiprion activities...
  12. pmc Accumulation of L-type bovine prions in peripheral nerve tissues
    Yoshifumi Iwamaru
    National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    Emerg Infect Dis 16:1151-4. 2010
    ..As with classical BSE prions, L-type BSE prions accumulated in central and peripheral nerve tissues...
  13. doi request reprint Quantitative analysis of wet-heat inactivation in bovine spongiform encephalopathy
    Yuichi Matsuura
    Prion Disease Research Center, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    Biochem Biophys Res Commun 432:86-91. 2013
    ..Our results show that BSE infectivity is strongly resistant to wet-heat inactivation and that it is necessary to pay attention to BSE decontamination in recycled cattle by-products...
  14. doi request reprint Sensitive detection of scrapie prion protein in soil
    Kazunari Nagaoka
    Soil Microbiology Research Team, National Agricultural Research Center, 3 1 1 Kannondai, Tsukuba, Ibaraki 305 8666, Japan
    Biochem Biophys Res Commun 397:626-30. 2010
    ..Our method appears to be a very useful technique for monitoring PrP(Sc) levels in soil...
  15. ncbi request reprint Examination of the offspring of a Japanese cow affected with L-type bovine spongiform encephalopathy
    Takashi Yokoyama
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki, Japan
    J Vet Med Sci 73:121-3. 2011
    ..Transmission of L-BSE was not observed during 4 years of observation, though the steer was born when the dam was in the terminal stages of the disease...
  16. ncbi request reprint Experimental transmission of two young and one suspended bovine spongiform encephalopathy (BSE) cases to bovinized transgenic mice
    Takashi Yokoyama
    Prion Disease Research Center, National Institute of Animal Health, Ibaraki 305 0856, Japan
    Jpn J Infect Dis 60:317-20. 2007
    ..The results of a dilution experiment using the classical BSE prion indicated that prion infectivity in these 3 cattle was below the detection limit of 10(3.0) LD(50)/g...
  17. ncbi request reprint Assessment of prion inactivation by combined use of Bacillus-derived protease and SDS
    Miyako Yoshioka
    Prion Disease Research Center, National Institute of Animal Health, Japan
    Biosci Biotechnol Biochem 71:2565-8. 2007
    ..The treatment therefore can be applied to the decontamination of thermolabile instruments...
  18. pmc The presence of disease-associated prion protein in skeletal muscle of cattle infected with classical bovine spongiform encephalopathy
    Hiroyuki Okada
    National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305 0856, Japan
    J Vet Med Sci 76:103-7. 2014
    ..The present data indicate that small amounts of PrP(Sc) are detectable by immunohistochemistry in the skeletal muscles of animals terminally affected with C-BSE. ..
  19. ncbi request reprint Fusarenon-X induced apoptosis in HL-60 cells depends on caspase activation and cytochrome c release
    Katsuhiro Miura
    Department of Safety Research, National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, 305, Ibaraki, Japan
    Toxicology 172:103-12. 2002
    ..These findings suggest that FX induces apoptosis in HL-60 cells by stimulating cytochrome c release followed by its downstream events including the activation of multiple caspases...
  20. ncbi request reprint Characterization of a proteolytic enzyme derived from a Bacillus strain that effectively degrades prion protein
    M Yoshioka
    National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    J Appl Microbiol 102:509-15. 2007
    ..This paper describes the biochemical characteristics and proteolytic activity of the isolated protease...
  21. ncbi request reprint Assessment of prion inactivation by fenton reaction using protein misfolding cyclic amplification and bioassay
    Kyozo Suyama
    Sports Nutrition Department, Sendai University, Funaoka Minami, Shibata, Japan
    Biosci Biotechnol Biochem 71:2069-71. 2007
    ..Therefore, the Fenton reaction has potential for prion decontamination...
  22. ncbi request reprint Prion inactivation by the Maillard reaction
    Kyozo Suyama
    Sports Nutrition Department, Sendai University, Miyagi, Japan
    Biochem Biophys Res Commun 356:245-8. 2007
    ..Therefore, the Maillard reaction can be employed for the decontamination of large amounts of byproducts such as MBM...