Masayuki Machida

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. ncbi request reprint Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering
    Junichiro Marui
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Higashi 1 1 1, AIST Tsukuba Central 6, Tsukuba 305 8566, Ibaraki, Japan
    J Biosci Bioeng 110:8-11. 2010
  2. pmc Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among Aspergillus species
    Myco Umemura
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Higashi Nijo 17 2 1, Tsukisamu, Sapporo, Hokkaido 062 8517, Japan
    DNA Res 19:375-82. 2012
  3. ncbi request reprint Genome sequencing and analysis of Aspergillus oryzae
    Masayuki Machida
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Nature 438:1157-61. 2005
  4. ncbi request reprint [Genome sequencing of Aspergillus oryzae]
    Masayuki Machida
    Tanpakushitsu Kakusan Koso 51:452-6. 2006
  5. doi request reprint Genomics of industrial Aspergilli and comparison with toxigenic relatives
    Masayuki Machida
    Research Institute of Cell Engineering, National Institute of Advanced Industrial Science and Technologies, Central 6, 1 1, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:1147-51. 2008
  6. pmc Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future
    Masayuki Machida
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, 1 1, Higashi, Tsukuba, Ibaraki, Japan
    DNA Res 15:173-83. 2008
  7. ncbi request reprint Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation
    Koichi Tamano
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Central 6, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Fungal Genet Biol 45:139-51. 2008
  8. doi request reprint Kojic acid biosynthesis in Aspergillus oryzae is regulated by a Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the transcriptional level
    Junichiro Marui
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Tsukuba, Ibaraki, Japan
    J Biosci Bioeng 112:40-3. 2011
  9. doi request reprint Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae
    Yasunobu Terabayashi
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Higashi 1 1 1, Tsukuba, Ibaraki, Japan
    Fungal Genet Biol 47:953-61. 2010
  10. ncbi request reprint Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus
    Myco Umemura
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 17 2 1 Higashi Nijo, Tsukisamu, Toyohira ku, Sapporo, Hokkaido 062 8517, Japan Electronic address
    Fungal Genet Biol 68:23-30. 2014

Detail Information

Publications36

  1. ncbi request reprint Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering
    Junichiro Marui
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Higashi 1 1 1, AIST Tsukuba Central 6, Tsukuba 305 8566, Ibaraki, Japan
    J Biosci Bioeng 110:8-11. 2010
    ..Overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production...
  2. pmc Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among Aspergillus species
    Myco Umemura
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Higashi Nijo 17 2 1, Tsukisamu, Sapporo, Hokkaido 062 8517, Japan
    DNA Res 19:375-82. 2012
    ..Our findings suggest that the high mutation frequency of NSBs might contribute to maintaining the diversity of the A. oryzae genome...
  3. ncbi request reprint Genome sequencing and analysis of Aspergillus oryzae
    Masayuki Machida
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Nature 438:1157-61. 2005
    ..Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation...
  4. ncbi request reprint [Genome sequencing of Aspergillus oryzae]
    Masayuki Machida
    Tanpakushitsu Kakusan Koso 51:452-6. 2006
  5. doi request reprint Genomics of industrial Aspergilli and comparison with toxigenic relatives
    Masayuki Machida
    Research Institute of Cell Engineering, National Institute of Advanced Industrial Science and Technologies, Central 6, 1 1, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:1147-51. 2008
    ..Expression analysis by the DNA microarray supported the significantly lower expression of genes on the non-syntenic than on the syntenic blocks...
  6. pmc Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future
    Masayuki Machida
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, 1 1, Higashi, Tsukuba, Ibaraki, Japan
    DNA Res 15:173-83. 2008
    ..oryzae. Genomic analysis in concert with traditional knowledge and technology will continue to be powerful tools in the future exploration of A. oryzae...
  7. ncbi request reprint Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation
    Koichi Tamano
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Central 6, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Fungal Genet Biol 45:139-51. 2008
    ..These results strongly suggest that the genes on NSBs play an important role on solid-state fermentation...
  8. doi request reprint Kojic acid biosynthesis in Aspergillus oryzae is regulated by a Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the transcriptional level
    Junichiro Marui
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Tsukuba, Ibaraki, Japan
    J Biosci Bioeng 112:40-3. 2011
    ..We determined that the gene was essential for KA production and the transcriptional activation of KA biosynthetic genes, which were triggered by the addition of KA...
  9. doi request reprint Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae
    Yasunobu Terabayashi
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Higashi 1 1 1, Tsukuba, Ibaraki, Japan
    Fungal Genet Biol 47:953-61. 2010
    ..These two genes are closely associated in the genome, showing typical characteristics of genes involved in secondary metabolism...
  10. ncbi request reprint Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus
    Myco Umemura
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 17 2 1 Higashi Nijo, Tsukisamu, Toyohira ku, Sapporo, Hokkaido 062 8517, Japan Electronic address
    Fungal Genet Biol 68:23-30. 2014
    ....
  11. ncbi request reprint The beta-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface
    Koichi Tamano
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan
    Biosci Biotechnol Biochem 71:926-34. 2007
    ..These data suggest that ExgA plays a role in beta-glucan utilization, but is not much involved in cell wall beta-glucan remodeling...
  12. pmc Fine de novo sequencing of a fungal genome using only SOLiD short read data: verification on Aspergillus oryzae RIB40
    Myco Umemura
    National Institute of Advanced Industrial Science and Technology AIST, Sapporo, Hokkaido, Japan
    PLoS ONE 8:e63673. 2013
    ..0 kb, and k-mer size 33...
  13. ncbi request reprint A novel vector for positive selection of inserts harboring an open reading frame by translational coupling
    Sumiko Ohashi-Kunihiro
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Higashi, Tsukuba, Ibaraki, Japan
    Biotechniques 43:751-2, 754. 2007
    ..Our method differs from previous methods in that the marker gene is activated without protein fusion, and that selection occurs irrespective of the reading frame of the insert...
  14. pmc Genome and transcriptome analysis of the basidiomycetous yeast Pseudozyma antarctica producing extracellular glycolipids, mannosylerythritol lipids
    Tomotake Morita
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 5 2, 1 1 1 Higashi, Tsukuba, Ibaraki, Japan
    PLoS ONE 9:e86490. 2014
    ..The analysis and dataset contribute to stimulate the development of improved strains with customized properties for high yield production of functional bio-based materials. ..
  15. doi request reprint Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes
    Koichi Tamano
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 2 17 2 1 Tsukisamu Higashi, Toyohira ku, Sapporo, Hokkaido, 062 8517, Japan
    Appl Microbiol Biotechnol 97:269-81. 2013
    ..Our data demonstrate that a simple increase in the abundance of fatty acid synthase genes can increase the detectable amount of fatty acids...
  16. ncbi request reprint A polymerase chain reaction-based method for cloning novel members of a gene family using a combination of degenerate and inhibitory primers
    Sumiko Kunihiro
    Institute of Molecular and Cell Biology, National Institute of Advanced Industrial Science and Technology, Central 6, 1 1, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Gene 289:177-84. 2002
    ....
  17. pmc MIDDAS-M: motif-independent de novo detection of secondary metabolite gene clusters through the integration of genome sequencing and transcriptome data
    Myco Umemura
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Sapporo, Hokkaido, Japan
    PLoS ONE 8:e84028. 2013
    ....
  18. ncbi request reprint Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector
    Hiroko Hagiwara
    Research Center for Glycoscience, Advanced Institute of Industrial Science and Technology, Central Higashi, Tsukuba, Ibaraki 305 8566, Japan
    J Biochem 132:975-82. 2002
    ..This result clearly indicates that the modified lambda phage display vector in combination with our enrichment technique has great potential for the enrichment of DNA-binding proteins in a sequence-specific manner...
  19. pmc Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids
    Tomotake Morita
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Tsukuba, Ibaraki, Japan
    Genome Announc 1:e0006413. 2013
    ..antarctica for industrial applications...
  20. pmc Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling
    Mohammed Naimuddin
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Mol Brain 4:2. 2011
    ..3F proteins are small (~7 kDa), structurally well defined, thermally stable and resistant to proteolysis that presents them as promising candidates for directed evolution...
  21. ncbi request reprint Progress of Aspergillus oryzae genomics
    Masayuki Machida
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Central 6, 1 1, Higashi, Tsukuba, Ibaraki, 305 8566, Japan
    Adv Appl Microbiol 51:81-106. 2002
  22. ncbi request reprint System-level identification of transcriptional circuits underlying mammalian circadian clocks
    Hiroki R Ueda
    Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Tsukuba, Ibaraki 305 8585, Japan
    Nat Genet 37:187-92. 2005
    ..Our analysis further suggests that regulation of E/E' boxes is a topological vulnerability in mammalian circadian clocks, a concept that has been functionally verified using in vitro phenotype assay systems...
  23. ncbi request reprint Construction of a positive selection marker by a lethal gene with the amber stop codon(s) regulator
    Sumiko Ohashi-Kunihiro
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Ibaraki, Japan
    Biosci Biotechnol Biochem 70:119-25. 2006
    ..The marker was also successfully applied to lambda phage display vector...
  24. ncbi request reprint A simple and reliable method for the detection of sialylation in complex/hybrid-type carbohydrate chains of glycoproteins by mixed lectins
    Jun Natsuki
    National Institute of Advanced Industrial Science and Technology AIST, Central 6, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Biotechnol Bioeng 93:225-30. 2006
    ..The reaction with the two lectins can be performed in a single tube and readily automated taking advantage of the use of paramagnetic beads...
  25. ncbi request reprint Improvement of the lectin-antibody enzyme immunoassay of the alphafetoprotein carbohydrate chain for automation with the enzyme immunoassay robot
    Koichi Tamano
    National Institute of Advanced Industrial Science and Technology AIST, Ibaraki, Japan
    Biosci Biotechnol Biochem 69:1616-9. 2005
    ..This indicates that the improved method is potentially applicable to the automated enzyme immunoassay robot...
  26. ncbi request reprint Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
    William C Nierman
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 438:1151-6. 2005
    ..The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus...
  27. ncbi request reprint Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
    James E Galagan
    The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
    Nature 438:1105-15. 2005
    ..These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation...
  28. pmc Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions
    Takeshi Akao
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima, Hiroshima 739 0046, Japan
    DNA Res 14:47-57. 2007
    ..In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories...
  29. ncbi request reprint Genomics of Aspergillus oryzae
    Tetsuo Kobayashi
    Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
    Biosci Biotechnol Biochem 71:646-70. 2007
    ..oryzae genome. Besides the various already established merits of A. oryzae for industrial uses, the genome sequence and the abundance of metabolic genes should significantly accelerate the biotechnological use of A. oryzae in industry...
  30. ncbi request reprint Impact of Aspergillus oryzae genomics on industrial production of metabolites
    Keietsu Abe
    Laboratory of Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, 981 8555, Japan
    Mycopathologia 162:143-53. 2006
    ..This article describes recent progress of A . oryzae genomics and its impact on industrial production of enzymes, metabolites, and bioprocesses...
  31. ncbi request reprint Deletion analysis of the superoxide dismutase (sodM) promoter from Aspergillus oryzae
    Hiromoto Hisada
    Research Institute, Gekkeikan Sake Co Ltd 101 Shimotoba koyanagi cho, Fushimi ku, Kyoto, 612 8361, Japan
    Appl Microbiol Biotechnol 72:1048-53. 2006
    ..To confirm these findings, we constructed enhanced sodM promoters by incorporating tandem repeats of this region, which resulted in an approximate twofold increase in expression relative to the native sodM promoter...
  32. ncbi request reprint Development of an automated SNP analysis method using a paramagnetic beads handling robot
    Hiroko Hagiwara
    Advanced Institute of Industrial Science and Technology AIST, Japan
    Biotechnol Bioeng 98:420-8. 2007
    ....
  33. ncbi request reprint Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags
    Hiroshi Maeda
    Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1 1 Amamiya, Tsutsumi dori, 981 8555 Sendai, Japan
    Appl Microbiol Biotechnol 65:74-83. 2004
    ..The low expression levels of catabolic genes in the wheat-bran culture may release catabolite repression, consequently leading to the rich expression profiles of the hydrolytic enzymes...
  34. ncbi request reprint Improvement of the Aspergillus oryzae enolase promoter (P-enoA) by the introduction of cis-element repeats
    Hirokazu Tsuboi
    General Research Laboratory, Ozeki Corporation, Hyogo, Japan
    Biosci Biotechnol Biochem 69:206-8. 2005
    ..The transformant harboring seven copies of the novel vector showed more than 100,000 U/mg GUS protein, which was approximately 30% of all the cell-free soluble proteins...
  35. ncbi request reprint Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae
    Hiroshi Maeda
    Tohoku Technoarch, 2 1 1 Katahira, Aoba ku, Sendai, 980 8577, Japan
    Appl Microbiol Biotechnol 67:778-88. 2005
    ..Determination of the affinities for different chemicals indicated that the most preferred substrate for the enzyme would consist of butyric acid and n-hexanol...
  36. ncbi request reprint Deletion analysis of the catalase-encoding gene (catB) promoter from Aspergillus oryzae
    Hiromoto Hisada
    Research Institute, Gekkeikan Sake Co, Ltd, Kyoto, Japan
    Biosci Biotechnol Biochem 72:48-53. 2008
    ..According to detailed promoter deletion analysis, a region from -1,000 to -975, which contains putative heat shock element (HSE) and the CCAAT-box, was involved in strong expression...