N Kiyokawa

Summary

Affiliation: National Research Institute for Child Health and Development
Country: Japan

Publications

  1. ncbi Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage
    Ban Sato
    Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, 2 10 1 Okura, Tokyo 157 8535, Japan
    BMC Dev Biol 11:22. 2011
  2. ncbi Induction of apoptosis in normal human renal tubular epithelial cells by Escherichia coli Shiga toxins 1 and 2
    N Kiyokawa
    Department of Pathology, National Children s Medical Research Center, Tokyo, Japan
    J Infect Dis 178:178-84. 1998
  3. ncbi Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma
    Nobutaka Kiyokawa
    Department of Developmental Biology, National Research Institute for Child Health and Development, Japan
    Mod Pathol 17:423-9. 2004
  4. ncbi Activation of the caspase cascade during Stx1-induced apoptosis in Burkitt's lymphoma cells
    N Kiyokawa
    Department of Pathology, National Children's Medical Research Center, Setagaya-ku, Tokyo 154-8509, Japan
    J Cell Biochem 81:128-42. 2001
  5. ncbi Single-step method for purification of Shiga toxin-1 B subunit using receptor-mediated affinity chromatography by globotriaosylceramide-conjugated octyl sepharose CL-4B
    H Nakajima
    Department of Pathology, National Children's Medical Research Center, Taishido, Tokyo, Japan
    Protein Expr Purif 22:267-75. 2001
  6. ncbi Kinetic analysis of binding between Shiga toxin and receptor glycolipid Gb3Cer by surface plasmon resonance
    H Nakajima
    Department of Pathology, National Children's Medical Research Center, 3-35-31, Taishido, Setagaya-ku, Tokyo 154-8509, Japan
    J Biol Chem 276:42915-22. 2001
  7. ncbi Nitrobenzylthioinosine (NBT), a nucleoside transport inhibitor, protects against Shiga toxin cytotoxicity in human microvascular endothelial cells
    K Ohmi
    Department of Pathology, National Children's Medical Research Center, Tokyo, Japan
    Endothelium 8:261-8. 2001
  8. ncbi Apoptosis of renal tubular cells in Shiga-toxin-mediated hemolytic uremic syndrome
    K Kaneko
    Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan
    Nephron 87:182-5. 2001
  9. ncbi Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d)
    N Sato
    Department of Pathology, National Children s Medical Research Center, Tokyo, Japan
    Hybridoma 19:455-61. 2000
  10. ncbi Costimulatory signals distinctively affect CD20- and B-cell-antigen-receptor-mediated apoptosis in Burkitt's lymphoma/leukemia cells
    K Mimori
    Department of Developmental Biology, National Research Institute for Child Health and Development, 3-35-31 Taishido, Setagaya-ku, Tokyo 154-8567, Japan
    Leukemia 17:1164-74. 2003

Collaborators

Detail Information

Publications45

  1. ncbi Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage
    Ban Sato
    Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, 2 10 1 Okura, Tokyo 157 8535, Japan
    BMC Dev Biol 11:22. 2011
    ....
  2. ncbi Induction of apoptosis in normal human renal tubular epithelial cells by Escherichia coli Shiga toxins 1 and 2
    N Kiyokawa
    Department of Pathology, National Children s Medical Research Center, Tokyo, Japan
    J Infect Dis 178:178-84. 1998
    ..coli infection...
  3. ncbi Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma
    Nobutaka Kiyokawa
    Department of Developmental Biology, National Research Institute for Child Health and Development, Japan
    Mod Pathol 17:423-9. 2004
    ..Our data indicate that CD179a and CD179b may be important markers for the immunophenotypic diagnosis of precursor B-cell lymphoblastic lymphomas...
  4. ncbi Activation of the caspase cascade during Stx1-induced apoptosis in Burkitt's lymphoma cells
    N Kiyokawa
    Department of Pathology, National Children's Medical Research Center, Setagaya-ku, Tokyo 154-8509, Japan
    J Cell Biochem 81:128-42. 2001
    ..Our findings should provide new insight into the understanding of the molecular basis of Stx1-mediated cell injury...
  5. ncbi Single-step method for purification of Shiga toxin-1 B subunit using receptor-mediated affinity chromatography by globotriaosylceramide-conjugated octyl sepharose CL-4B
    H Nakajima
    Department of Pathology, National Children's Medical Research Center, Taishido, Tokyo, Japan
    Protein Expr Purif 22:267-75. 2001
    ..This versatile purification method can be used to isolate various types of natural as well as recombinant Stx, facilitating fundamental studies of human diseases caused by this toxin...
  6. ncbi Kinetic analysis of binding between Shiga toxin and receptor glycolipid Gb3Cer by surface plasmon resonance
    H Nakajima
    Department of Pathology, National Children's Medical Research Center, 3-35-31, Taishido, Setagaya-ku, Tokyo 154-8509, Japan
    J Biol Chem 276:42915-22. 2001
    ..The data described herein clearly demonstrate differences between the binding properties of Stx1 and Stx2 and may facilitate understanding of the differences in clinical manifestations caused by these toxins...
  7. ncbi Nitrobenzylthioinosine (NBT), a nucleoside transport inhibitor, protects against Shiga toxin cytotoxicity in human microvascular endothelial cells
    K Ohmi
    Department of Pathology, National Children's Medical Research Center, Tokyo, Japan
    Endothelium 8:261-8. 2001
    ..NBT also protected against Stx1 cytotoxicity in sodium butyrate-treated hypersensitive HMVECs. The protective effect of NBT against Stx cytotoxicity may be due to the depletion of ATP in the cells, thereby inhibiting the entry of Stx1...
  8. ncbi Apoptosis of renal tubular cells in Shiga-toxin-mediated hemolytic uremic syndrome
    K Kaneko
    Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan
    Nephron 87:182-5. 2001
    ....
  9. ncbi Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d)
    N Sato
    Department of Pathology, National Children s Medical Research Center, Tokyo, Japan
    Hybridoma 19:455-61. 2000
    ..This study established 1C2 as a MAb against mouse and rat GPV, namely CD42d, and as useful tool to study rodent megakaryopoiesis...
  10. ncbi Costimulatory signals distinctively affect CD20- and B-cell-antigen-receptor-mediated apoptosis in Burkitt's lymphoma/leukemia cells
    K Mimori
    Department of Developmental Biology, National Research Institute for Child Health and Development, 3-35-31 Taishido, Setagaya-ku, Tokyo 154-8567, Japan
    Leukemia 17:1164-74. 2003
    ..CD20-mediated apoptosis in BL cells may provide not only a model for understanding the mechanism regulating clonal selection of B cells but a new therapeutic strategy for BL patients...
  11. ncbi CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system
    T Suzuki
    Department of Pathology, National Children s Medical Research Center, Tokyo, Japan
    J Immunol 166:5567-77. 2001
    ..CD24-mediated apoptosis in BL cells may provide a model for the cell death mechanism initiated by GEM-associated molecules, which is closely related to B cell receptor for Ag-mediated apoptosis...
  12. ncbi A role for lipid rafts in immune cell signaling
    Y U Katagiri
    Department of Pathology, National Children's Medical Research Center, Tokyo, Japan
    Microbiol Immunol 45:1-8. 2001
    ..Rafts are suggested to be important in controlling appropriate protein interactions in hematopoietic cells, and aggregation of rafts following receptor ligation may be a general mechanism for promoting immune cell signaling...
  13. ncbi Characterization of new monoclonal antibodies against porcine lymphocytes: molecular characterization of clone 7G3, an antibody reactive with the constant region of the T-cell receptor delta-chains
    W-R Tang
    Department of Developmental Biology, National Research Institute for Child Health and Development, 3-35-31 Taishido, Setagaya-ku, Tokyo 154-8567, Japan
    Vet Immunol Immunopathol 103:113-27. 2005
    ..This report precisely characterizes mAbs against porcine TCR for the first time, facilitating molecular biological investigations of the porcine immune system...
  14. ncbi Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity
    Hiroshi Nakao
    Department of Infectious Diseases, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo 154 8567, Japan
    Microbiol Immunol 46:777-80. 2002
    ....
  15. ncbi Laminin binding protein, 34/67 laminin receptor, carries stage-specific embryonic antigen-4 epitope defined by monoclonal antibody Raft.2
    Yohko U Katagiri
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo 157 8535, Japan
    Biochem Biophys Res Commun 332:1004-11. 2005
    ..An SSEA-4-positive human EC cell line, NCR-G3 cells, also expressed Raft.2-binding LBP...
  16. ncbi The detergent-insoluble microdomains, rafts, can be used as an effective immunogen
    Yohko U Katagiri
    Department of Developmental Biology, National Research Institute for Child Health and Development, 2 10 1 Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Glycoconj J 25:495-501. 2008
    ..Our findings suggest that this phenomenon does not represent a breakdown of immunological self-tolerance, but typical immune reactions accompanying the class switch from IgM antibodies to IgG antibodies...
  17. ncbi Neurofibromatosis 2 tumor suppressor, the gene induced by valproic acid, mediates neurite outgrowth through interaction with paxillin
    Junji Yamauchi
    Department of Pharmacology, National Research Institute for Child Health and Development, 2 10 1 Okura, Setagaya, Tokyo 157 8535, Japan
    Exp Cell Res 314:2279-88. 2008
    ..These results suggest that merlin is an as yet unknown regulator of neurite outgrowth through the interaction with paxillin, providing a possibly common mechanism regulating neurite formation...
  18. ncbi Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development
    Tomoko Taguchi
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo, Japan
    Exp Hematol 34:508-18. 2006
    ..By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development...
  19. ncbi Human osteoblasts support hematopoietic cell development in vitro
    Yusuke Shiozawa
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
    Acta Haematol 120:134-45. 2008
    ..The ability of human osteoblasts to support early hematopoiesis was investigated...
  20. ncbi Shiga toxin binding to globotriaosyl ceramide induces intracellular signals that mediate cytoskeleton remodeling in human renal carcinoma-derived cells
    Hisami Takenouchi
    Department of Developmental Biology, National Research Institute for Child Health and Development, 3-35-31, Taishido, Setagaya-ku, Tokyo, 154-8567, Japan
    J Cell Sci 117:3911-22. 2004
    ....
  21. ncbi Interleukin-7 contributes to human pro-B-cell development in a mouse stromal cell-dependent culture system
    Tomoko Taguchi
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo, Japan
    Exp Hematol 35:1398-407. 2007
    ..The role of interleukin (IL)-7 in human B lymphopoiesis is still controversial. We used an in vitro culture system to verify involvement of IL-7 in development of human pro-B cells from hematopoietic stem cells...
  22. ncbi Characterization of a shiga-toxin 1-resistant stock of vero cells
    Takaomi Sekino
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
    Microbiol Immunol 48:377-87. 2004
    ..Further study of R-Vero cells may provide a model of Stx1 resistance via distinct intracellular transport of Stx1...
  23. ncbi Deficiency of BLNK hampers PLC-gamma2 phosphorylation and Ca2+ influx induced by the pre-B-cell receptor in human pre-B cells
    Tomoko Taguchi
    Department of Developmental Biology, National Research Institute for Child Health and Development, 3 35 31 Taishido, Setagaya Ku, Tokyo 154 8567, Japan
    Immunology 112:575-82. 2004
    ..Human pre-B cell lines expressing and not expressing BLNK should provide an in vitro model for investigation of the role of BLNK in the pre-BCR-mediated signalling mechanism...
  24. ncbi The expression of granulysin in systemic anaplastic large cell lymphoma in childhood
    Noriko Kitamura
    Department of Developmental Biology, National Research Institute for Child Health and Development, 2 10 1 Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Leuk Res 33:908-12. 2009
    ..We further demonstrated that both ALCL cells in biopsy specimens and cell lines established from ALCL express granulysin, indicating some correlation of granulysin with biological features of ALCL...
  25. ncbi Differential effects of BAFF on B cell precursor acute lymphoblastic leukemia and Burkitt lymphoma
    Keiko Onda
    Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, 2 10 1 Okura, Setagaya Ku, Tokyo, 157 8535, Japan
    Int J Hematol 91:808-19. 2010
    ..The results of this study indicate that some BCP-ALL cells and some BL cells express BAFF-R, but that the effects of BAFF on BCP-ALL cells are different from its effects on mature B cell malignancies...
  26. ncbi B-cell-activating factor inhibits CD20-mediated and B-cell receptor-mediated apoptosis in human B cells
    Yohei Saito
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo, Japan
    Immunology 125:570-90. 2008
    ..MLMA cells should provide a model for investigating the molecular basis of the effect of BAFF on B cells in vitro and will help to elucidate how B cells survive in the immune system in which BAFF-mediated signalling is involved...
  27. ncbi Characterization of monocyte-macrophage-lineage cells induced from CD34+ bone marrow cells in vitro
    Kyoko Suzuki
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
    Int J Hematol 85:384-9. 2007
    ..Monocyte-macrophage-lineage cells induced in vitro should provide an ideal model for functional analysis of monocyte-macrophage cells...
  28. ncbi EWS/ETS regulates the expression of the Dickkopf family in Ewing family tumor cells
    Yoshitaka Miyagawa
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo, Japan
    PLoS ONE 4:e4634. 2009
    ..Recent studies have revealed the involvement of this family in tumorigenesis, however their role in tumorigenesis is still remained unclear...
  29. ncbi Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos
    Ban Sato
    Department of Developmental Biology, National Research Institute for Child Health and Development, 2 1 0 1 Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Biochem Biophys Res Commun 364:838-43. 2007
    ..These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2...
  30. ncbi Pre-B cell antigen receptor-mediated signal inhibits CD24-induced apoptosis in human pre-B cells
    Tomoko Taguchi
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
    J Immunol 170:252-60. 2003
    ..Our findings should be important to understanding the role of CD24-mediated cell signaling in early B cell development...
  31. ncbi Raft.1, a monoclonal antibody raised against the raft microdomain, recognizes G-protein beta1 and 2, which assemble near nucleus after shiga toxin binding to human renal cell line
    Yohko U Katagiri
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
    Lab Invest 82:1735-45. 2002
    ..Because Gbeta1 and 2 are highly conserved all through organisms and are deeply involved in signal transduction, Raft.1 is expected to be utilized frequently in research...
  32. ncbi Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain
    Wei-Ran Tang
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan
    Hybrid Hybridomics 23:187-91. 2004
    ....
  33. ncbi Inducible expression of chimeric EWS/ETS proteins confers Ewing's family tumor-like phenotypes to human mesenchymal progenitor cells
    Yoshitaka Miyagawa
    Department of Developmental Biology, National Research Institute for Child Health and Development, 2 10 1, Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Mol Cell Biol 28:2125-37. 2008
    ..Together with the observation that EWS/ETS enhances the ability of cells to invade Matrigel, these results suggest that EWS/ETS proteins contribute to alterations of cellular features and confer an EFT-like phenotype to human MPCs...
  34. ncbi Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin
    Yoshitaka Miyagawa
    Department of Developmental Biology, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo, Japan
    Immunology 128:405-19. 2009
    ..The findings of Foxp3 expression, a characteristic of regulatory T cells, and a low level of IL-17 gene expression suggest that CB-derived CD4(+) T cells may be a more appropriate source for DLI...
  35. ncbi Mesenchymal to embryonic incomplete transition of human cells by chimeric OCT4/3 (POU5F1) with physiological co-activator EWS
    Hatsune Makino
    Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, 157 8535, Japan
    Exp Cell Res 315:2727-40. 2009
    ....
  36. ncbi Kinetics and effect of integrin expression on human CD34(+) cells during murine leukemia virus-derived retroviral transduction with recombinant fibronectin for stem cell gene therapy
    Yasuomi Horiuchi
    Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo 157 8535, Japan
    Hum Gene Ther 20:777-83. 2009
    ..Our findings should be useful for improving the culture conditions for CH-296-based MLV-derived retroviral transduction in stem cell gene therapy...
  37. ncbi Primary malignant lymphoma of the central nervous system in an immunocompetent child: a case report
    Yusuke Shiozawa
    Department of Pediatrics, Juntendo University, School of Medicine, Tokyo, Japan
    J Pediatr Hematol Oncol 27:561-4. 2005
    ..Despite its rarity, PCNSL should be included in the differential diagnosis in the presence of symptoms of increased intracranial pressure and/or unusual imaging findings of the brain...
  38. ncbi Granulocytic sarcoma of the spine in a child without bone marrow involvement: a case report and literature review
    Yusuke Shiozawa
    Department of Paediatrics, Juntendo University School of Medicine, Tokyo, Japan
    Eur J Pediatr 164:616-20. 2005
    ..CONCLUSION: Our patient is the youngest case of granulocytic sarcoma of the spine without bone marrow involvement. Immunohistochemical methods are very helpful in establishing a diagnosis of granulocytic sarcoma...
  39. ncbi Inhibition of shiga toxin cytotoxicity in human renal cortical epithelial cells by nitrobenzylthioinosine
    Takaomi Sekino
    Department of Pathology, National Children's Medical Research Center, Setagaya-ku, Tokyo, Japan
    J Infect Dis 185:785-96. 2002
    ..Investigation of the NBTI-mediated protection mechanism against Stx cytotoxicity may provide insights into the analysis of Stx-mediated cell damage and lead to improvements in therapeutic approaches for diseases caused by Stx...
  40. ncbi CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1
    Chizu Nonomura
    Cell Regulation Analysis Team, Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
    Cancer Res 68:790-9. 2008
    ....
  41. ncbi Kinetics of HCV envelope proteins' interaction with CD81 large extracellular loop
    Hideki Nakajima
    Division of Oncology, Department of Medicine, Stanford University Medical Center, Stanford, CA, USA
    Biochem Biophys Res Commun 328:1091-100. 2005
    ..It also implies that the heterogeneity is likely due to the HCV envelope proteins, which are known to form non-covalently linked heterodimers and disulfide-linked aggregate...
  42. ncbi Cloning and characterization of porcine common gamma chain gene
    Daisuke Honma
    Genome Research Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 0901, Japan
    J Interferon Cytokine Res 23:101-11. 2003
    ..The results indicate that the Ets-1 binding motif in the segment from -95 to -59 (major transcription initiation site: +1) was an essential cis-acting regulatory element for the common gamma chain gene in lymphoid cells...
  43. ncbi [Retrograde transport of Shiga toxin 1 in human renal tubular epithelial cells]
    Nobutaka Kiyokawa
    Department of Pathology, National Children's Medical Research Center
    Nippon Rinsho 60:1095-100. 2002
    ..Investigation of retrograde transport mechanism of Stx should provide insights into the analysis of Stx-mediated cell damage and lead to improvements in therapeutic approaches for diseases caused by Stx...
  44. ncbi Catalytic RAG1 mutants obstruct V(D)J recombination in vitro and in vivo
    Tadashi Furusawa
    Developmental Biology Department, National Institute of Agrobiological Sciences, Ikenodai 2, Kukisaki, Inashiki, Ibaraki 305-8602, Japan
    Mol Immunol 39:871-8. 2003
    ..Our results suggest that immunodeficient animals for regenerative medicine could be generated without gene knockout...
  45. ncbi Development of diffuse large B cell lymphoma during the maintenance therapy for B-lineage acute lymphoblastic leukemia
    Itaru Kato
    Department of Pediatrics, St Luke s International Hospital, 9 1 Akashi cho, Chuo Ku, Tokyo 104 8560, Japan
    Pediatr Blood Cancer 48:230-2. 2007
    ..DLBL was treated by surgical resection followed by chemotherapy for 6 months. The patient has been free from the recurrence of ALL or DLBL for 16 months after the development of DLBL...