Miho Kawahata

Summary

Affiliation: National Research Institute of Brewing
Country: Japan

Publications

  1. ncbi request reprint Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:17-28. 2002
  2. ncbi request reprint Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:393-402. 2002
  3. ncbi request reprint Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p
    Miho Kawahata
    National Research Institute of Brewing, Kagamiyama, Higashi Hiroshima, Japan
    FEMS Yeast Res 6:924-36. 2006
  4. ncbi request reprint Intraspecies diversity of the industrial yeast strains Saccharomyces cerevisiae and Saccharomyces pastorianus based on analysis of the sequences of the internal transcribed spacer (ITS) regions and the D1/D2 region of 26S rDNA
    Miho Kawahata
    National Research Institute of Brewing, Kagamiyama, Higashi Hiroshima, Japan
    Biosci Biotechnol Biochem 71:1616-20. 2007

Detail Information

Publications4

  1. ncbi request reprint Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:17-28. 2002
    ..The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain...
  2. ncbi request reprint Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:393-402. 2002
    ..The counter-selectable integrating plasmids and the gene disruption cassettes can allow the markers to be used repeatedly for yeast gene manipulations...
  3. ncbi request reprint Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p
    Miho Kawahata
    National Research Institute of Brewing, Kagamiyama, Higashi Hiroshima, Japan
    FEMS Yeast Res 6:924-36. 2006
    ..Moreover, our quantitative PCR showed that expression of PDR12 increased under acid shock response with lactic acid and decreased under acid adaptation with hydrochloric acid...
  4. ncbi request reprint Intraspecies diversity of the industrial yeast strains Saccharomyces cerevisiae and Saccharomyces pastorianus based on analysis of the sequences of the internal transcribed spacer (ITS) regions and the D1/D2 region of 26S rDNA
    Miho Kawahata
    National Research Institute of Brewing, Kagamiyama, Higashi Hiroshima, Japan
    Biosci Biotechnol Biochem 71:1616-20. 2007
    ..And the beer and whisky yeasts had two thymines at position B and one cytosine at position C. Strains of S. pastorianus were divided into three groups based on the sequences of their 26S rDNA D1/D2 and ITS regions...