Sunil C Kaul

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. ncbi request reprint CARF regulates p19ARF-p53-p21WAF1 senescence pathway by multiple checkpoints
    Sunil C Kaul
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Ann N Y Acad Sci 1067:217-9. 2006
  2. ncbi request reprint Stress chaperones, mortalin, and pex19p mediate 5-aza-2' deoxycytidine-induced senescence of cancer cells by DNA methylation-independent pathway
    Nashi Widodo
    National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1, Higashi, Tsukuba, Ibaraki, Japan
    J Gerontol A Biol Sci Med Sci 62:246-55. 2007
  3. ncbi request reprint Overexpressed mortalin (mot-2)/mthsp70/GRP75 and hTERT cooperate to extend the in vitro lifespan of human fibroblasts
    Sunil C Kaul
    National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Exp Cell Res 286:96-101. 2003
  4. pmc Proproliferative functions of Drosophila small mitochondrial heat shock protein 22 in human cells
    Renu Wadhwa
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    J Biol Chem 285:3833-9. 2010
  5. ncbi request reprint Upregulation of mortalin/mthsp70/Grp75 contributes to human carcinogenesis
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Int J Cancer 118:2973-80. 2006
  6. pmc Identification and characterization of molecular interactions between mortalin/mtHsp70 and HSP60
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Biochem J 391:185-90. 2005
  7. doi request reprint Glycerol stimulates innate chaperoning, proteasomal and stress-resistance functions: implications for geronto-manipulation
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, 305 8562, Japan
    Biogerontology 9:269-82. 2008
  8. ncbi request reprint Quantum dot-based protein imaging and functional significance of two mitochondrial chaperones in cellular senescence and carcinogenesis
    Zeenia Kaul
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Ann N Y Acad Sci 1067:469-73. 2006
  9. ncbi request reprint CARF (collaborator of ARF) interacts with HDM2: evidence for a novel regulatory feedback regulation of CARF-p53-HDM2-p21WAF1 pathway
    M Kamrul Hasan
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Int J Oncol 32:663-71. 2008
  10. ncbi request reprint Activation of wild type p53 function by its mortalin-binding, cytoplasmically localizing carboxyl terminus peptides
    Sunil C Kaul
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    J Biol Chem 280:39373-9. 2005

Collaborators

Detail Information

Publications57

  1. ncbi request reprint CARF regulates p19ARF-p53-p21WAF1 senescence pathway by multiple checkpoints
    Sunil C Kaul
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Ann N Y Acad Sci 1067:217-9. 2006
    ..In the nucleoplasm, CARF interacts with p53 and enhances its function. We demonstrate that p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2...
  2. ncbi request reprint Stress chaperones, mortalin, and pex19p mediate 5-aza-2' deoxycytidine-induced senescence of cancer cells by DNA methylation-independent pathway
    Nashi Widodo
    National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1, Higashi, Tsukuba, Ibaraki, Japan
    J Gerontol A Biol Sci Med Sci 62:246-55. 2007
    ..We conclude that both mortalin and Pex19p are important mediators, prognostic indicators, and tailoring tools for 5AZA-dC-induced senescence in cancer cells...
  3. ncbi request reprint Overexpressed mortalin (mot-2)/mthsp70/GRP75 and hTERT cooperate to extend the in vitro lifespan of human fibroblasts
    Sunil C Kaul
    National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Exp Cell Res 286:96-101. 2003
    ..Together, mot-2 and hTERT permitted bypass of senescence, a substantial extension of lifespan, and possibly immortalization. This is the first demonstration that mot-2 and telomerase can cooperate in the immortalization process...
  4. pmc Proproliferative functions of Drosophila small mitochondrial heat shock protein 22 in human cells
    Renu Wadhwa
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    J Biol Chem 285:3833-9. 2010
    ..We report that the DmHsp22 interacts and inactivates wild type tumor suppressor protein p53, which may be one possible way of its functioning in human cells...
  5. ncbi request reprint Upregulation of mortalin/mthsp70/Grp75 contributes to human carcinogenesis
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Int J Cancer 118:2973-80. 2006
    ..The study demonstrates that upregulation of mortalin contributes significantly to tumorigenesis, and thus is a good candidate target for cancer therapy...
  6. pmc Identification and characterization of molecular interactions between mortalin/mtHsp70 and HSP60
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Biochem J 391:185-90. 2005
    ....
  7. doi request reprint Glycerol stimulates innate chaperoning, proteasomal and stress-resistance functions: implications for geronto-manipulation
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, 305 8562, Japan
    Biogerontology 9:269-82. 2008
    ..These results may have significant implications in the use of glycerol as a candidate geronto-modulator in development of practical interventions for "healthy aging"...
  8. ncbi request reprint Quantum dot-based protein imaging and functional significance of two mitochondrial chaperones in cellular senescence and carcinogenesis
    Zeenia Kaul
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Ann N Y Acad Sci 1067:469-73. 2006
    ..We demonstrate the minute differences in subcellular distribution of mortalin and HSP60, their involvement in tumorigenesis, and functional distinction in pathways involved in senescence...
  9. ncbi request reprint CARF (collaborator of ARF) interacts with HDM2: evidence for a novel regulatory feedback regulation of CARF-p53-HDM2-p21WAF1 pathway
    M Kamrul Hasan
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Int J Oncol 32:663-71. 2008
    ..By overexpression and silencing studies, we demonstrated that CARF exerts a vital control on the p53-HDM2-p21WAF1 pathway that is frequently altered in cancer cells...
  10. ncbi request reprint Activation of wild type p53 function by its mortalin-binding, cytoplasmically localizing carboxyl terminus peptides
    Sunil C Kaul
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    J Biol Chem 280:39373-9. 2005
    ..Such activation of p53 function was sufficient to cause growth arrest of human osteosarcoma and breast carcinoma cells...
  11. ncbi request reprint Functional significance of minor structural and expression changes in stress chaperone mortalin
    Custer C Deocaris
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Ann N Y Acad Sci 1119:165-75. 2007
    ..Besides being validated as a reliable target for cancer therapy, mortalin also warrants attention from the perspectives of management of old-age diseases and healthy aging...
  12. doi request reprint Deceleration of senescence in normal human fibroblasts by withanone extracted from ashwagandha leaves
    Nashi Widodo
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Gerontol A Biol Sci Med Sci 64:1031-8. 2009
    ..To the best of our knowledge, we provide the first example of phytochemical(s) (i-Extract and withanone) that have both anticancer and antiaging activities and point to the molecular link between aging and cancer...
  13. pmc Ashwagandha leaf derived withanone protects normal human cells against the toxicity of methoxyacetic acid, a major industrial metabolite
    Didik Priyandoko
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    PLoS ONE 6:e19552. 2011
    ..These findings warrant further basic and clinical studies that may promote the use of withanone as a health adjuvant in a variety of consumer products where the toxicity has been a concern because of the use of ester phthalates...
  14. pmc Alternative reading frame protein (ARF)-independent function of CARF (collaborator of ARF) involves its interactions with p53: evidence for a novel p53-activation pathway and its negative feedback control
    Md Kamrul Hasan
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Biochem J 380:605-10. 2004
    ....
  15. doi request reprint Selective killing of cancer cells by leaf extract of Ashwagandha: components, activity and pathway analyses
    Nashi Widodo
    National Institute of Advanced Industrial Science and Technology AIST, Central, Higashi, Tsukuba, Ibaraki, Japan
    Cancer Lett 262:37-47. 2008
    ....
  16. ncbi request reprint A novel putative collaborator of p19ARF
    Renu Wadhwa
    Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Exp Gerontol 38:245-52. 2003
    ..CARF is a novel binding partner of ARF and might be involved in its p53-dependent or -independent tumor suppressive functions...
  17. doi request reprint CARF Is a vital dual regulator of cellular senescence and apoptosis
    Kamrul Hasan
    National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    J Biol Chem 284:1664-72. 2009
    ..We provide evidence that CARF plays a dual role in regulating p53-mediated senescence and apoptosis, the two major tumor suppressor mechanisms...
  18. doi request reprint Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells: combinational approach for enhanced differentiation
    Navjot Shah
    National Institute of Advanced Industrial Science and Technology, University if Tsukuba, Ibaraki, Japan
    Cancer Sci 100:1740-7. 2009
    ..These assays led us to formulate a unique combination formula of i-Extract components that caused enhanced differentiation of glial cells...
  19. ncbi request reprint CARF is a novel protein that cooperates with mouse p19ARF (human p14ARF) in activating p53
    Md Kamrul Hasan
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    J Biol Chem 277:37765-70. 2002
    ..It co-localizes and interacts with ARF in the nucleolus. We demonstrate that CARF is co-regulated with ARF, cooperates with it in activating p53, and thus acts as a novel component of the ARF-p53-p21 pathway...
  20. doi request reprint Fate of bone marrow mesenchymal stem cells following the allogeneic transplantation of cartilaginous aggregates into osteochondral defects of rabbits
    Tomokazu Yoshioka
    Department of Orthopaedic Surgery, University of Tsukuba, Ibaraki, Japan
    J Tissue Eng Regen Med 5:437-43. 2011
    ..The results suggest that i-MSCs did not inhibit, and indeed contributed to, the regeneration of osteochondral defects...
  21. doi request reprint CARF: an emerging regulator of p53 tumor suppressor and senescence pathway
    Caroline T Y Cheung
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Mech Ageing Dev 130:18-23. 2009
    ..The aim of this article is to discuss the current knowledge about functions of CARF and its impact on p53 pathway in regulation of senescence and carcinogenesis...
  22. ncbi request reprint Reduction in mortalin level by its antisense expression causes senescence-like growth arrest in human immortalized cells
    Renu Wadhwa
    National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    J Gene Med 6:439-44. 2004
    ..We demonstrate that when the expression of mortalin (a member of hsp70 family proteins) was suppressed in such genetically defined immortal cells they underwent a permanent growth arrest...
  23. ncbi request reprint Quantum dot-based mortalin staining as a visual assay for detection of induced senescence in cancer cells
    Zeenia Kaul
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Ann N Y Acad Sci 1100:368-72. 2007
    ..Based on the superior resolution, stability and inertness, we propose the use of QD staining of mortalin as a cell-based visual assay to screen for senescence-inducing drugs, proteins, and siRNAs...
  24. ncbi request reprint Mortalin-MPD (mevalonate pyrophosphate decarboxylase) interactions and their role in control of cellular proliferation
    Renu Wadhwa
    National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Biochem Biophys Res Commun 302:735-42. 2003
    ..Ras and its oncogenic forms act as key players in controlling proliferation of normal and cancerous cells. Assigning mot-2 upstream of p21(Ras) offers an important mechanism for influence over cell proliferation...
  25. pmc Targeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymes
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki, 305 8566, Japan
    EMBO Rep 4:595-601. 2003
    ....
  26. ncbi request reprint CARF binds to three members (ARF, p53, and HDM2) of the p53 tumor-suppressor pathway
    Hasan M Kamrul
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Ann N Y Acad Sci 1100:312-5. 2007
    ..CARF may exert a vital control on p53-HDM2-p21(WAF1) pathway that is central to the cell cycle control, senescence, and DNA damage response of human cells...
  27. ncbi request reprint Involvement of mortalin in cellular senescence from the perspective of its mitochondrial import, chaperone, and oxidative stress management functions
    Tomoko Yaguchi
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Ann N Y Acad Sci 1100:306-11. 2007
    ....
  28. ncbi request reprint Selective killing of cancer cells by leaf extract of Ashwagandha: identification of a tumor-inhibitory factor and the first molecular insights to its effect
    Nashi Widodo
    Research Institute for Cell Engineering, GENE Therapeutics, Inc, National Institute of Advanced Industrial Science and Technology, Higashi, Tsukuba, Japan
    Clin Cancer Res 13:2298-306. 2007
    ..We undertook a study to identify and characterize the antitumor activity of the leaf extract of ashwagandha...
  29. ncbi request reprint Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates
    Zeenia Kaul
    National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 3058562, Japan
    Cell Res 13:503-7. 2003
    ..Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable...
  30. pmc Merger of ayurveda and tissue culture-based functional genomics: inspirations from systems biology
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    J Transl Med 6:14. 2008
    ....
  31. ncbi request reprint Mortalin sensitizes human cancer cells to MKT-077-induced senescence
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Cancer Lett 252:259-69. 2007
    ..Our work may likely translate to a re-appraisal of the therapeutic benefits of low doses of several classes of anti-tumor drugs, even of those that had been discontinued due to adverse effects...
  32. ncbi request reprint Internalizing antibody-based targeted gene delivery for human cancer cells
    Maki Shiota
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Hum Gene Ther 18:1153-60. 2007
    ....
  33. ncbi request reprint Use of a randomized hybrid ribozyme library for identification of genes involved in muscle differentiation
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    J Biol Chem 279:51622-9. 2004
    ..This functional gene screening is not only helpful in understanding the molecular pathways of muscle differentiation but also to design molecular strategies for myopathologic therapies...
  34. doi request reprint From proliferative to neurological role of an hsp70 stress chaperone, mortalin
    Custer C Deocaris
    Institute of Health and Sports Science, University of Tsukuba, Ibaraki, 305 8574, Japan
    Biogerontology 9:391-403. 2008
    ..Inversely, it may also seem that vulnerability to mitochondrial dysfunction could be precipitated by malevolent (anti-chaperone) gain-of-function of a 'sick mortalin'...
  35. ncbi request reprint Geroprotection by glycerol: insights to its mechanisms and clinical potentials
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Japan
    Ann N Y Acad Sci 1067:488-92. 2006
    ..This was accompanied by decrease in p53, an upregulation of a stress chaperone mortalin/mtHsp70, and an increase in proteasome activity in the presence of oxidative stress...
  36. pmc Selective killing of cancer cells by Ashwagandha leaf extract and its component Withanone involves ROS signaling
    Nashi Widodo
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    PLoS ONE 5:e13536. 2010
    ..In the present study, we investigated its mechanism by loss-of-function screening (abrogation of i-Extract induced cancer cell killing) of the cellular targets and gene pathways...
  37. doi request reprint Molecular bridging of aging and cancer: A CARF link
    Caroline T Cheung
    National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan
    Ann N Y Acad Sci 1197:129-33. 2010
    ..Most recently, we have found that CARF causes bidirectional regulation of p53 and pRB pathways, either arresting or promoting growth, and thus, it could be a potential threshold link between aging and cancer...
  38. pmc MicroRNA-296 is enriched in cancer cells and downregulates p21WAF1 mRNA expression via interaction with its 3' untranslated region
    A Rum Yoon
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Nucleic Acids Res 39:8078-91. 2011
    ..We demonstrate for the first time that miR-296 is frequently upregulated during immortalization of human cells and contributes to carcinogenesis by downregulation of p53-p21(WAF1) pathway...
  39. ncbi request reprint Three faces of mortalin: a housekeeper, guardian and killer
    Sunil C Kaul
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Exp Gerontol 42:263-74. 2007
    ..Being proved to be an attractive target for cancer therapy, it also warrants attention from the perspectives of management of old age diseases and healthy aging...
  40. ncbi request reprint Structural and functional differences between mouse mot-1 and mot-2 proteins that differ in two amino acids
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Japan
    Ann N Y Acad Sci 1067:220-3. 2006
    ..Here, we provide evidence that mot-1 possesses very low chaperone activity as compared to mot-2. A "lazy lid" hypothesis is proposed for their differential aging phenotypes...
  41. pmc Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3
    Ping Dai
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Ibaraki, Japan
    Genes Dev 16:2843-8. 2002
    ..Furthermore, a Ski mutation enhanced the digit abnormalities caused by the Gli3 gene mutation. Thus, Ski plays an important role in pattern formation...
  42. ncbi request reprint A major functional difference between the mouse and human ARF tumor suppressor proteins
    Renu Wadhwa
    Gene Function Research Laboratory, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    J Biol Chem 277:36665-70. 2002
    ..In view of the major role of ARF in tumor suppression, this distinction may contribute to the different levels of tumor proneness of these species...
  43. pmc Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) promotes tumor metastasis by induction of genes involved in extracellular matrix, cell movement, and angiogenesis
    Ran Gao
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    J Biol Chem 288:15046-56. 2013
    ..Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis. Our results demonstrated that the hnRNP-K is a potential target for metastasis therapy...
  44. ncbi request reprint Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein
    Renu Wadhwa
    Chugai Research Institute for Medical Sciences, 153 2 Nagai, Niihari Mura, Ibaraki, 300 41, Japan
    Exp Cell Res 274:246-53. 2002
    ..Therefore, perturbation of mot-p53 interactions can be employed to abrogate cytoplasmic retention of wild-type p53 in tumors...
  45. ncbi request reprint Mortalin: present and prospective
    Sunil C Kaul
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Exp Gerontol 37:1157-64. 2002
    ..Depending on its different subcellular niche and binding partner therein, mortalin is expected to perform multiple functions relevant to cell survival, control of proliferation and stress response...
  46. ncbi request reprint Quantum dots in bio-imaging: Revolution by the small
    Harinder Arya
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Biochem Biophys Res Commun 329:1173-7. 2005
    ..Within a decade, research on QDs has evolved from being a pure science subject to the one with high-end commercial applications...
  47. doi request reprint Stable and nondisruptive in vitro/in vivo labeling of mesenchymal stem cells by internalizing quantum dots
    Yoshimi Ohyabu
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Hum Gene Ther 20:217-24. 2009
    ..The i-QD-labeled MSCs underwent normal adipocyte, osteocyte, and chondrocyte differentiation in vitro and in vivo, suggesting the potential application of i-QDs in in vivo diagnostics, regenerative and therapeutic medicine...
  48. ncbi request reprint Vectors for RNA interference
    Renu Wadhwa
    National Institute of Advanced Industrial Science and Technology, Gene Function Research Center, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Curr Opin Mol Ther 6:367-72. 2004
    ..This review describes the basis for, and methodology of siRNA expression vectors for mammalian cells...
  49. ncbi request reprint The versatile stress protein mortalin as a chaperone therapeutic agent
    Custer C Deocaris
    Institute of Health and Sports Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba, Japan
    Protein Pept Lett 16:517-29. 2009
    ..could be creatively applied in a clinical setting to manage stress and to treat various chaperone-based maladies or "chaperonopathies"...
  50. ncbi request reprint Druggability of mortalin for cancer and neuro-degenerative disorders
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Curr Pharm Des 19:418-29. 2013
    ..As we demystify its deux de machina, accumulating evidence reveal that mortalin may be "druggable" bidirectionally to either treat cancer or neuro-degenerative disorders...
  51. ncbi request reprint Identification of metastasis-related genes in a mouse model using a library of randomized ribozymes
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 279:38083-6. 2004
    ..The further characterization of these candidate genes would provide clues to the complex mechanism(s) of metastasis...
  52. ncbi request reprint Rhodacyanine dye MKT-077 inhibits in vitro telomerase assay but has no detectable effects on telomerase activity in vivo
    Renu Wadhwa
    Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology, Ibaraki 305 8566, Japan
    Cancer Res 62:4434-8. 2002
    ..The data suggested that inhibition of telomerase activity is unlikely to be a prime cause of MKT-077-induced toxicity in cancer cells...
  53. ncbi request reprint LIM kinase-2 targeting as a possible anti-metastasis therapy
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    J Gene Med 6:357-63. 2004
    ..In this study, we have determined the effect of ribozymes specific for LIM kinase-2 on metastatic and proliferative phenotypes of human fibrosarcoma cells...
  54. ncbi request reprint Dose and dose-rate effects of low-dose ionizing radiation on activation of Trp53 in immortalized murine cells
    Takashi Sugihara
    Department of Radiobiology, Institute for Environmental Sciences, 1 7 Ienomae, Obuchi, Rokkasho, Kamikita, Aomori 039 3212, Japan
    Radiat Res 162:296-307. 2004
    ..These results show that cellular response to radiation depended on the dose rate used; i.e., the responses seen at dose rates from 0.1-1 cGy/h were different from those observed at dose rates over 5 cGy/h...
  55. ncbi request reprint Know-how of RNA interference and its applications in research and therapy
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial, Science and Technology, 1 1 1 Higashi, Tsukuba 305 8562, Japan
    Mutat Res 567:71-84. 2004
    ..There is no doubt that further progress in understanding the mechanism of their action as well as strategies to achieve their tightly regulated and tissue specific expression will revolutionize basic and applied biomedical research...
  56. ncbi request reprint Imminent approaches towards molecular interventions in ageing
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    Mech Ageing Dev 126:481-90. 2005
    ..These post-genomic technologies will help us in the discovery of gene targets for interventions aiming to improve the quality at later years of life beyond their mere algebraic extension...
  57. ncbi request reprint Use of ribozymes in cellular aging research
    Custer C Deocaris
    National Institute of Advanced Indutrial Science and Technology AIST, Tsukuba Scince City, Japan
    Methods Mol Biol 371:209-26. 2007
    ....