Haruo Kasai

Summary

Affiliation: National Institute for Physiological Sciences
Country: Japan

Publications

  1. ncbi request reprint Fast and cAMP-sensitive mode of Ca(2+)-dependent exocytosis in pancreatic beta-cells
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Diabetes 51:S19-24. 2002
  2. pmc A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:891-903. 2005
  3. ncbi request reprint Structure-stability-function relationships of dendritic spines
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University for Advanced Studies SOKENDAI, Okazaki 444 8585, Japan
    Trends Neurosci 26:360-8. 2003
  4. ncbi request reprint [Structure-stability-function relationships of dendritic spines]
    Haruo Kasai
    Tanpakushitsu Kakusan Koso 49:276-81. 2004
  5. pmc Sequential exocytosis of insulin granules is associated with redistribution of SNAP25
    Noriko Takahashi
    Dept of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    J Cell Biol 165:255-62. 2004
  6. pmc Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets
    Hiroyasu Hatakeyama
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    J Physiol 570:271-82. 2006
  7. pmc Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis
    Takuya Kishimoto
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:905-15. 2005
  8. pmc Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis
    Ting Ting Liu
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:917-29. 2005
  9. pmc Structural basis of long-term potentiation in single dendritic spines
    Masanori Matsuzaki
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University of Advanced Studies Sokendai, Myodaiji, Okazaki 444 8787, Japan
    Nature 429:761-6. 2004
  10. ncbi request reprint Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet
    Noriko Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, and the Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    Science 297:1349-52. 2002

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Fast and cAMP-sensitive mode of Ca(2+)-dependent exocytosis in pancreatic beta-cells
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Diabetes 51:S19-24. 2002
    ..In contrast to the fast component of exocytosis, the slow component (mode 2) of this process is independent of cAMP and ATP and can account for the slow component of insulin secretion, which does not require these nucleotides...
  2. pmc A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:891-903. 2005
    ..It allows quantitative imaging of exocytosis within secretory organs, yielding estimates of vesicle diameter with nanometer resolution...
  3. ncbi request reprint Structure-stability-function relationships of dendritic spines
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University for Advanced Studies SOKENDAI, Okazaki 444 8585, Japan
    Trends Neurosci 26:360-8. 2003
    ..Characterization of supramolecular complexes responsible for synaptic memory and learning is key to the understanding of brain function and disease...
  4. ncbi request reprint [Structure-stability-function relationships of dendritic spines]
    Haruo Kasai
    Tanpakushitsu Kakusan Koso 49:276-81. 2004
  5. pmc Sequential exocytosis of insulin granules is associated with redistribution of SNAP25
    Noriko Takahashi
    Dept of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    J Cell Biol 165:255-62. 2004
    ..These observations support the hypothesis that SNAP25 is a plasma membrane factor that is responsible for sequential exocytosis...
  6. pmc Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets
    Hiroyasu Hatakeyama
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    J Physiol 570:271-82. 2006
    ....
  7. pmc Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis
    Takuya Kishimoto
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:905-15. 2005
    ....
  8. pmc Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis
    Ting Ting Liu
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:917-29. 2005
    ..Thus, we have, for the first time, directly visualized massive exocytosis of small vesicles in a non-synaptic preparation, and have revealed their fusion-pore mediated exocytosis and endocytosis...
  9. pmc Structural basis of long-term potentiation in single dendritic spines
    Masanori Matsuzaki
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University of Advanced Studies Sokendai, Myodaiji, Okazaki 444 8787, Japan
    Nature 429:761-6. 2004
    ..They further suggest that small spines are preferential sites for long-term potentiation induction, whereas large spines might represent physical traces of long-term memory...
  10. ncbi request reprint Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet
    Noriko Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, and the Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    Science 297:1349-52. 2002
    ..When the pore dilated to 12 nanometers, the granules rapidly flattened and discharged their contents. Thus, our methodology reveals fusion pore dynamics in intact tissues at nanometer resolution...
  11. pmc Spine-neck geometry determines NMDA receptor-dependent Ca2+ signaling in dendrites
    Jun Noguchi
    Department of Cell Physiology, National Institute for Physiological Sciences and Graduate University of Advanced Studies SOKENDAI, Myodaiji, Okazaki 444 8787, Japan
    Neuron 46:609-22. 2005
    ..Spine-neck geometry is thus an important determinant of spine Ca2+ signaling, allowing small spines to be the preferential sites for isolated induction of long-term potentiation...
  12. pmc A novel function of Noc2 in agonist-induced intracellular Ca2+ increase during zymogen-granule exocytosis in pancreatic acinar cells
    Sho Ogata
    Department of Cell Physiology, National Institute for Physiological Sciences, and Graduate University of Advanced Studies, SOKENDAI, Okazaki, Aichi, Japan
    PLoS ONE 7:e37048. 2012
    ..We propose that in pancreatic acinar cells, Noc2 is not indispensable for the membrane fusion of ZG per se, but instead performs a novel function favoring agonist-induced physiological [Ca(2+)](i) increases...
  13. ncbi request reprint Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands
    Akihiro Oshima
    Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies SOKENDAI, 5 1 Higashiyama, Myodaiji cho, Okazaki, Aichi 444 8787, Japan
    Cell Calcium 37:349-57. 2005
    ..The delay in sequential compound exocytosis relative to fluid secretion may be important for release of the viscous contents of secretory granules into the nasal cavity...
  14. ncbi request reprint [Dendritic spine structures and functions]
    Haruo Kasai
    Departement of Cell Physiology, National Institute for Physiological Sciences, Okazaki, 444 8585 Japan
    Nihon Shinkei Seishin Yakurigaku Zasshi 22:159-64. 2002
    ..These observations suggest that a physical basis of the memory in the cerebral cortex resides in the alteration and maintenance of spine structures...
  15. ncbi request reprint Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines
    Masamichi Ohkura
    Department of Information Physiology, and Department of Cell Physiology, National Institute for Physiological Sciences, and School of Life Science, Graduate University for Advanced Studies, Okazaki, Aichi 444 8585, Japan
    Anal Chem 77:5861-9. 2005
    ..6 shifted the affinity for Ca2+ and reduced the Ca2+-buffering effect. G-CaMP1.6-CaM(E140K), which has a mutation in the Ca2+ binding site, is an improved probe with its increased brightness and reduced Ca2+-buffering capacity...
  16. ncbi request reprint Stabilization of exocytosis by dynamic F-actin coating of zymogen granules in pancreatic acini
    Tomomi Nemoto
    Department of Cell Physiology, National Institute for Physiological Sciences, and Graduate University of Advanced Studies, 5 1 Higashiyama, Myodaiji cho, Okazaki, Aichi 444 8787, Japan
    J Biol Chem 279:37544-50. 2004
    ..Rho-dependent F-actin coating of granule membranes thus stabilizes exocytic structures and is necessary for physiological progression of sequetial compound exocytosis in the exocrine pancreas and for prevention of acute pancreatitis...
  17. ncbi request reprint Two-photon excitation imaging of pancreatic islets with various fluorescent probes
    Noriko Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Diabetes 51:S25-8. 2002
    ..In addition, our data suggest that membrane-permeable antagonists may affect only the superficial cell layers of islets, and so their negative effects should be interpreted with caution...
  18. ncbi request reprint Rapid Ca2+-dependent increase in oxygen consumption by mitochondria in single mammalian central neurons
    Yasuyuki Hayakawa
    Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    Cell Calcium 37:359-70. 2005
    ..This process might function as a rapid feed-forward mechanism in homeostatic control of the cytosolic ATP concentration...
  19. doi request reprint Spatiotemporal dynamics of functional clusters of neurons in the mouse motor cortex during a voluntary movement
    Riichiro Hira
    Division of Brain Circuits, National Institute for Basic Biology and the Graduate University of Advanced Studies Sokendai, Myodaiji, Okazaki 444 8585, Japan
    J Neurosci 33:1377-90. 2013
    ..This higher fidelity of activity was related to higher trial-to-trial correlations of activities of pairs within the cluster. We propose that strong recurrent network clusters may represent the execution of voluntary movements...
  20. ncbi request reprint Switch to anaerobic glucose metabolism with NADH accumulation in the beta-cell model of mitochondrial diabetes. Characteristics of betaHC9 cells deficient in mitochondrial DNA transcription
    Mitsuhiko Noda
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Japan
    J Biol Chem 277:41817-26. 2002
    ..This is the first report to demonstrate the connection between metabolic alteration of electron transport system and that of tricarboxylic acid cycle and its impact on insulin secretion...
  21. pmc Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines
    Jun ichi Tanaka
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Science 319:1683-7. 2008
    ..Thus, this spike-timing protocol is an efficient trigger for BDNF secretion and induces protein synthesis-dependent long-term enlargement at the level of single spines...
  22. doi request reprint Three-dimensional mapping of unitary synaptic connections by two-photon macro photolysis of caged glutamate
    Masanori Matsuzaki
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    J Neurophysiol 99:1535-44. 2008
    ..Thus this technique will be a uniquely powerful tool for quantifying synaptic connectivity and manipulating neural activity at the single-cell level...
  23. pmc Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in mouse beta-cells
    Hiroyasu Hatakeyama
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Bunkyo ku, Tokyo, 113 0033 Japan
    J Physiol 582:1087-98. 2007
    ..Thus, Epac and PKA selectively regulate exocytosis of SVs and LVs, respectively, in beta-cells, and Epac can regulate exocytosis more rapidly than PKA...
  24. ncbi request reprint Exocytic process analyzed with two-photon excitation imaging in endocrine pancreas
    Noriko Takahashi
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Japan
    Endocr J 54:337-46. 2007
    ..Two-photon imaging will be powerful tools to elucidate molecular and cellular mechanisms of exocytosis and related disease, and to develop new therapeutic agencies as well as diagnostic tools...
  25. ncbi request reprint The HNF-1 target collectrin controls insulin exocytosis by SNARE complex formation
    Kenji Fukui
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita Osaka 565 0871, Japan
    Cell Metab 2:373-84. 2005
    ..Therefore, collectrin is a regulator of SNARE complex function, which thereby controls insulin exocytosis...
  26. pmc Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla
    Takuya Kishimoto
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Hongo, Tokyo, Japan
    EMBO J 25:673-82. 2006
    ..We suggest the existence of two molecular configurations for fusion-ready states in Ca2+ -dependent exocytosis...
  27. ncbi request reprint Hepatocyte nuclear factor-4alpha is essential for glucose-stimulated insulin secretion by pancreatic beta-cells
    Atsuko Miura
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:5246-57. 2006
    ..Because the K(ATP) channel is important for proper insulin secretion in beta-cells, altered K(ATP) channel activity could be related to the impaired insulin secretion in the betaHNF-4alphaKO mice...
  28. pmc Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation
    Kazuo Kasai
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    J Clin Invest 115:388-96. 2005
    ....
  29. ncbi request reprint Propagation of gammaPKC translocation along the dendrites of Purkinje cell in gammaPKC-GFP transgenic mice
    Norio Sakai
    Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    Genes Cells 9:945-57. 2004
    ..The findings may provide us with a new insight for understanding molecular mechanisms of the synaptic plasticity including cerebellar long-term depression...
  30. doi request reprint The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines
    Naoki Honkura
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Neuron 57:719-29. 2008
    ..Ca2+/calmodulin-dependent protein kinase II regulated this confinement. Thus, spines have an elaborate mechanical nature that is regulated by actin fibers...
  31. ncbi request reprint Number and density of AMPA receptors in single synapses in immature cerebellum
    Jun ichi Tanaka
    Department of Physiological Sciences, Graduate University for Advanced Studies, Sokendai 444 8787, Japan
    J Neurosci 25:799-807. 2005
    ..Our results indicate that in this developing synapse, the efficacy of transmission is determined by the synaptic area...
  32. pmc 4-Carboxymethoxy-5,7-dinitroindolinyl-Glu: an improved caged glutamate for expeditious ultraviolet and two-photon photolysis in brain slices
    Graham C R Ellis-Davies
    Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Neurosci 27:6601-4. 2007
  33. pmc Bernard Katz, quantal transmitter release and the foundations of presynaptic physiology
    George J Augustine
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, University of Tokyo Faculty of Medicine, Tokyo, Japan
    J Physiol 578:623-5. 2007
  34. ncbi request reprint Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization
    Haruo Kasai
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Adv Drug Deliv Rev 58:850-77. 2006
    ....