Research Topics
| Yoshihiro KakuSummaryAffiliation: National Institute of Infectious Diseases Country: Japan Publications
| Collaborators
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Detail Information
Publications
Altered specificity of single-chain antibody fragments bound to pandemic H1N1-2009 influenza virus after conversion of the phage-bound to the soluble formYoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
BMC Res Notes 5:483. 2012..Aiming to establish H1N1pdm-specific detection tools rapidly, we employed phage display libraries to select H1N1pdm-specific single-chain variable fragments (scFvs)...
Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunizationYoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
Arch Virol 157:1605-9. 2012..5 × 10(4) TCID(50). Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay...
[Nipah virus infection]Yoshihiro Kaku
Department of Veterinary Science, National Institute of Infections Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
Uirusu 54:237-42. 2004..In this article, the epidemiological comparison between two outbreaks in Malaysia and Bangladesh, and the new-trends of virological studies of NiV will be discussed...
Antigenic properties of porcine teschovirus 1 (PTV-1) Talfan strain and molecular strategy for serotyping of PTVsY Kaku
Department of Exotic Diseases, National Institute of Animal Health, Tokyo, Japan
Arch Virol 152:929-40. 2007..This RT-PCR-based strategy appears to be a reliable surrogate for serotyping and could facilitate the diagnosis and epidemiological study of PTV infection...
Inhibition of rabies virus propagation in mouse neuroblastoma cells by an intrabody against the viral phosphoproteinYoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama, Shinjuku, Tokyo, Japan
Antiviral Res 91:64-71. 2011..Although the mechanism of RABV inhibition by scFv-P19 is not clear, this scFv-based intracellular immunization could be a candidate for future RABV therapeutic studies if combined with appropriate delivery and application systems...
A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent proteinYoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama, Shinjuku, Tokyo, Japan
J Virol Methods 160:7-13. 2009....
Second generation of pseudotype-based serum neutralization assay for Nipah virus antibodies: sensitive and high-throughput analysis utilizing secreted alkaline phosphataseYoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
J Virol Methods 179:226-32. 2012..The results suggest that this novel VSV-NiV-SEAP assay is safe, useful for high-throughput screening of sera using an ELISA plate reader, and has high sensitivity and specificity...
Gene expression analysis of host innate immune responses in the central nervous system following lethal CVS-11 infection in miceNaoko Sugiura
Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan
Jpn J Infect Dis 64:463-72. 2011..The presence of perforin and granzymes both in the Purkinje cells and CD3 T lymphocytes strongly suggested that apoptosis of the former cells was induced by the latter cells...
Effect of cellular cholesterol depletion on rabies virus infectionKozue Hotta
Department of Veterinary Science, National Institute of Infectious Diseases, 162 8640 Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
Virus Res 139:85-90. 2009..These results suggest that RABV enters BHK-21 or HEp-2 cells through ports of entry other than those located on cholesterol-rich microdomains and raise the possibility that RABV uses different mechanisms to enter the non-neuronal cells...
[Diagnostic tests: Nipah virus (NiV)]Yoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases
Nippon Rinsho 63:324-5. 2005
Immunohistochemical distribution of viral antigens in pigs naturally infected with porcine teschovirusManabu Yamada
National Institute of Animal Health, Tsukuba, Ibaraki, Japan
J Vet Med Sci 70:305-8. 2008..In the other examined organ, PTV antigens were observed in bronchiolar epithelial cells in the lung, hepatocytes in the liver, epithelial cells in the tonsils and the myenteric nerve plexus in the small and large intestine...
[Nipah virus infection]Yoshihiro Kaku
Department of Veterinary Science, National Institute of Infectious Diseases
Nippon Rinsho 65:154-6. 2007
Polymerase chain reaction-based genetic typing of Japanese porcine reproductive and respiratory syndrome virusesMasaaki Yoshii
National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki, Japan
J Vet Diagn Invest 16:342-7. 2004..These mismatches appeared to be responsible for the nonreactivity of the former primers to these viruses...
Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 talfanYoshihiro Kaku
Department of Infectious Diseases, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan
J Virol 76:11721-8. 2002..The PTV IRES has a unique set of properties and represents a new class of picornavirus IRES element...
