Research Topics
| Jun IshikawaSummaryAffiliation: National Institute of Infectious Diseases Country: Japan Publications
| Collaborators
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Detail Information
Publications
Construction of pRES18 and pRES19, Streptomyces-Escherichia coli shuttle vectors carrying multiple cloning sitesJ Ishikawa
Department of Bioactive Molecules, National Institute of Health, Tokyo, Japan
FEMS Microbiol Lett 145:113-6. 1996..coli (beta-lactamase (bla) and beta-galactosidase (lacZ). These shuttle vectors are capable of carrying DNA fragments as long as 10 kb, of being maintained in S. griseus, S. lavendulae and S. lividans, and are compatible with pIJ702...- FramePlot: a new implementation of the frame analysis for predicting protein-coding regions in bacterial DNA with a high G + C contentJ Ishikawa
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan
FEMS Microbiol Lett 174:251-3. 1999..These sequences can then be compared to the NCBI sequence database over the Internet. The program is freely available for academic purposes at http://www.nih.go.jp/jun/cgi-bin/frameplot.pl... 
Identification and characterization of the point mutation which affects the transcription level of the chromosomal 3-N-acetyltransferase gene of Streptomyces griseus SS-1198J Ishikawa
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
Antimicrob Agents Chemother 44:437-40. 2000..Therefore, it seemed very likely that the aac(3)-Xa gene was activated by the substitution resulting in the emergence of a stronger promoter...- Contribution of rpoB2 RNA polymerase beta subunit gene to rifampin resistance in Nocardia speciesJun Ishikawa
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
Antimicrob Agents Chemother 50:1342-6. 2006..Since this is the first example of genetic engineering of the Nocardia genome, we believe that this study, as well as our determination of the N. farcinica genome sequence, will be a landmark in Nocardia genetics... - The complete genomic sequence of Nocardia farcinica IFM 10152Jun Ishikawa
Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162 8640, Japan
Proc Natl Acad Sci U S A 101:14925-30. 2004..Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease... - Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strainsKeiko Ishino
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Clin Microbiol 45:607-9. 2007..aureus. PCR-restriction fragment length polymorphism typing of the coagulase gene was a more reliable method than coagulase serotyping from the viewpoint of arbekacin resistance... 
Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistanceYasutaka Hoshino
Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
J Antibiot (Tokyo) 63:23-8. 2010..farcinica...- Construction of a pair of practical Nocardia-Escherichia coli shuttle vectorsKazuhiro Chiba
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
Jpn J Infect Dis 60:45-7. 2007..These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp... - Identification of nocobactin NA biosynthetic gene clusters in Nocardia farcinicaYasutaka Hoshino
Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162 8640, Japan
J Bacteriol 193:441-8. 2011..The ΔnbtE mutant was found to be impaired in cytotoxicity against J774A.1 cells, suggesting that nocobactin NA production is required for virulence of N. farcinica... - Trends of arbekacin-resistant MRSA strains in Japanese hospitals (1979 to 2000)Naofumi Tsuchizaki
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan
J Antibiot (Tokyo) 59:229-33. 2006..If the predominance of type L21 continues, there will be no progression to ABK resistance in MRSA. However, it may be necessary to monitor the trends in type M22 continuously... - Cloning of the gene cluster responsible for the biosynthesis of brasilicardin A, a unique diterpenoidYutaka Hayashi
Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
J Antibiot (Tokyo) 61:164-74. 2008..We did not, however, detect any genes related to L-rhamnose and N-acetylglucosamine biosyntheses in the flanking regions. A gene disruption experiment did indeed show that this gene cluster was responsible for BCA biosynthesis... - The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humansYuko Sasaki
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1, Gakuen, Musashimurayama, Tokyo, Japan
Nucleic Acids Res 30:5293-300. 2002..Thus, M.penetrans HF-2 may have acquired diverse repertoires of antigenic variation-related genes to allow its persistent infection in humans... - Characterization of a bifunctional aminoglycoside-modifying enzyme with novel substrate specificity and its gene from a clinical isolate of methicillin-resistant Staphylococcus aureus with high arbekacin resistanceKeiko Ishino
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Antibiot (Tokyo) 57:679-86. 2004..Therefore, the cloned aac(6')/aph(2") gene may represent the molecular basis for the novel aminoglycoside modification capability as well as novel aminoglycoside resistance profile of S. aureus PRC104... - A Phase I/II study of nilotinib in Japanese patients with imatinib-resistant or -intolerant Ph+ CML or relapsed/refractory Ph+ ALLArinobu Tojo
The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
Int J Hematol 89:679-88. 2009..The results of this study confirmed the efficacy and safety of nilotinib in Japanese patients... - Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilisHaruo Ikeda
Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan
Nat Biotechnol 21:526-31. 2003..In contrast, the terminal regions were not conserved and preferentially contained nonessential genes... - Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350Yasuo Ohnishi
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
J Bacteriol 190:4050-60. 2008..griseus... - YM-341619 suppresses the differentiation of spleen T cells into Th2 cells in vitro, eosinophilia, and airway hyperresponsiveness in rat allergic modelsKeiko Ohga
Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc, 21 Miyukigaoka, Tsukuba shi, Ibaraki 305 8585, Japan
Eur J Pharmacol 590:409-16. 2008..These results suggest that YM-341619 has the ability to suppress allergen-induced Th2 responses by selectively inhibiting the differentiation of CD4(+) T cells into the Th2 subset... - An alternative menaquinone biosynthetic pathway operating in microorganismsTomoshige Hiratsuka
Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
Science 321:1670-3. 2008..As humans and commensal intestinal bacteria, including lactobacilli, lack this pathway, it represents an attractive target for the development of chemotherapeutics... - Characterization of immunoglobulin light chain isotypes in the common carpMitsuru Tomana
Department of Applied Biological Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
Immunogenetics 54:120-9. 2002..6-kb transcripts, possibly corresponding to fully spliced (LVJC) and truncated (JC/J-C) forms, respectively... - Nocardia terpenica sp. nov., isolated from Japanese patients with nocardiosisYasutaka Hoshino
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
Int J Syst Evol Microbiol 57:1456-60. 2007..nov. is proposed for the two strains. The type strain is IFM 0706(T) (=JCM 13033(T)=DSM 44935(T)=NBRC 100888(T))... - [The genome sequence of soil bacterium Streptomyces]Haruo Ikeda
Tanpakushitsu Kakusan Koso 47:1845-50. 2002 - Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalismKenji Ueda
Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
Nucleic Acids Res 32:4937-44. 2004..The genomic information from S.thermophilum offers new insights into microbial diversity and evolutionary sciences, and provides a framework for characterizing the molecular basis underlying microbial commensalism... - Transvalencin Z, a new antimicrobial compound with salicylic acid residue from Nocardia transvalensis IFM 10065Akira Mukai
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan
J Antibiot (Tokyo) 59:366-9. 2006..Transvalencin Z shows a strong antimicrobial activity against Gram-positive bacteria, but shows no activity against Gram-negative bacteria, fungi and tumor cells... - A pyrazole derivative, YM-58483, potently inhibits store-operated sustained Ca2+ influx and IL-2 production in T lymphocytesJun Ishikawa
Inflammation Research, Pharmacology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, Tsukuba shi, Ibaraki, Japan
J Immunol 170:4441-9. 2003..Furthermore, YM-58483 would be a candidate for the study of capacitative Ca(2+) entry mechanisms through SOC/CRAC channels and for identification of putative Ca(2+) channel genes... - Transvalencin A, a thiazolidine zinc complex antibiotic produced by a clinical isolate of Nocardia transvalensis. II. Structure elucidationYasutaka Hoshino
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Chiba 260-8673, Japan
J Antibiot (Tokyo) 57:803-7. 2004..Transvalencin A is comprised of o-substituted p-chlorophenol, tetrasubstituted oxazoline, disubstituted thiazolyl-N-methylthiazolidine and monosubstituted N-methylthiazolidine... - Transvalencin A, a thiazolidine zinc complex antibiotic produced by a clinical isolate of Nocardia transvalensis. I. Taxonomy, fermentation, isolation and biological activitiesYasutaka Hoshino
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba, Chiba 260-8673, Japan
J Antibiot (Tokyo) 57:797-802. 2004..The antibiotic is also active against Gram-positive bacteria such as Micrococcus luteus. We observed higher activity for fungi in an acidic medium than in a neutral medium... - Characterisation of a fourth immunoglobulin light chain isotype in the common carpJun Ishikawa
Department of Applied Biological Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan
Fish Shellfish Immunol 16:369-79. 2004..Southern blot analyses suggested that the locus of carp L2 has a cluster-like organisation. Phylogenetic analyses showed that both carp VL2 and CL2 amino acid sequences highly clustered with other teleost L2 sequences... - Characterisation of T cell antigen receptor alpha chain isotypes in the common carpEtsuou Imai
Department of Applied Biological Science, Nihon University, Fujisawa, Kanagawa, Japan
Fish Shellfish Immunol 19:205-16. 2005..Therefore, the results obtained using an individual clearly showed that carp possesses at least two Calpha loci, possibly as a result of tetraploidisation...