Jun Ishikawa

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. ncbi request reprint FramePlot: a new implementation of the frame analysis for predicting protein-coding regions in bacterial DNA with a high G + C content
    J Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan
    FEMS Microbiol Lett 174:251-3. 1999
  2. ncbi request reprint Construction of pRES18 and pRES19, Streptomyces-Escherichia coli shuttle vectors carrying multiple cloning sites
    J Ishikawa
    Department of Bioactive Molecules, National Institute of Health, Tokyo, Japan
    FEMS Microbiol Lett 145:113-6. 1996
  3. pmc Identification and characterization of the point mutation which affects the transcription level of the chromosomal 3-N-acetyltransferase gene of Streptomyces griseus SS-1198
    J Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 44:437-40. 2000
  4. pmc Contribution of rpoB2 RNA polymerase beta subunit gene to rifampin resistance in Nocardia species
    Jun Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 50:1342-6. 2006
  5. pmc The complete genomic sequence of Nocardia farcinica IFM 10152
    Jun Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162 8640, Japan
    Proc Natl Acad Sci U S A 101:14925-30. 2004
  6. pmc Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains
    Keiko Ishino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Clin Microbiol 45:607-9. 2007
  7. doi request reprint Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance
    Yasutaka Hoshino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    J Antibiot (Tokyo) 63:23-8. 2010
  8. ncbi request reprint Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors
    Kazuhiro Chiba
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Jpn J Infect Dis 60:45-7. 2007
  9. pmc Identification of nocobactin NA biosynthetic gene clusters in Nocardia farcinica
    Yasutaka Hoshino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162 8640, Japan
    J Bacteriol 193:441-8. 2011
  10. ncbi request reprint Trends of arbekacin-resistant MRSA strains in Japanese hospitals (1979 to 2000)
    Naofumi Tsuchizaki
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    J Antibiot (Tokyo) 59:229-33. 2006

Collaborators

  • Keiko Ishino
  • Yuzuru Mikami
  • K Hotta
  • Toshio Fukai
  • A Yamashita
  • Yasuhiro Igarashi
  • Nobuya Itoh
  • Tohru Dairi
  • Susan Branford
  • Yasushi Miyazaki
  • T Kenri
  • Junko H Ohyashiki
  • Tim P Hughes
  • Haruo Ikeda
  • Kenji Ueda
  • Miki Nakao
  • Yasutaka Hoshino
  • Katsukiyo Yazawa
  • Akira Mukai
  • Kazuhiro Chiba
  • Etsuou Imai
  • Mitsuru Tomana
  • Arinobu Tojo
  • Yutaka Hayashi
  • Fumiko Saito
  • Keiko Ohga
  • Tomoshige Hiratsuka
  • Yasuo Ohnishi
  • Takahisa Kogure
  • Naofumi Tsuchizaki
  • Masahira Hattori
  • Jun Uno
  • Akikazu Ando
  • Tadaaki Moritomo
  • Yuko Sasaki
  • Hideki Shinonaga
  • Hiroyuki Satoh
  • Shoko Fujii
  • Koshi Arai
  • Itsuro Jinnai
  • Kazuma Ohyashiki
  • Tatsuya Kawaguchi
  • Kensuke Usuki
  • Miki Nishimura
  • Masaya Okada
  • Hideo Tanaka
  • Yukio Kobayashi
  • Yasuhiro Maeda
  • Hiromi Tanii
  • Koichi Miyamura
  • Tomoki Naoe
  • Hiroko Natori
  • Akio Urabe
  • Taro Amagasaki
  • Shinichiro Okamoto
  • Noriko Usui
  • Hirofumi Hara
  • Ryuichi Takezawa
  • Yasuaki Shimizu
  • Mako Numazaki
  • Haruo Seto
  • Hiroaki Toshima
  • Miwa Ikenoya
  • Kazuo Furihata
  • Shinya Nagashima
  • Sadao Kuromitsu
  • Hirokazu Suzuki
  • Nobuyasu Matsuura
  • Sueharu Horinouchi
  • Haruyuki Yamashita
  • Yoshimasa Uehara
  • Takuji Kudo
  • Kayo Watanabe
  • Reiner M Kroppenstedt
  • Shin ichi Suzuki
  • Soji Iida
  • Mutsuyasu Nakajima
  • Yuko Matsumoto
  • Ken ichi Harada
  • Kentaro Yamaguchi
  • Tsuguo Sasaki
  • Chie Yoshino
  • Atsuko Horino
  • Kenshiro Oshima
  • Tadayoshi Shiba
  • Keiko Furuya
  • Tomoki Yano

Detail Information

Publications28

  1. ncbi request reprint FramePlot: a new implementation of the frame analysis for predicting protein-coding regions in bacterial DNA with a high G + C content
    J Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan
    FEMS Microbiol Lett 174:251-3. 1999
    ..These sequences can then be compared to the NCBI sequence database over the Internet. The program is freely available for academic purposes at http://www.nih.go.jp/jun/cgi-bin/frameplot.pl...
  2. ncbi request reprint Construction of pRES18 and pRES19, Streptomyces-Escherichia coli shuttle vectors carrying multiple cloning sites
    J Ishikawa
    Department of Bioactive Molecules, National Institute of Health, Tokyo, Japan
    FEMS Microbiol Lett 145:113-6. 1996
    ..coli (beta-lactamase (bla) and beta-galactosidase (lacZ). These shuttle vectors are capable of carrying DNA fragments as long as 10 kb, of being maintained in S. griseus, S. lavendulae and S. lividans, and are compatible with pIJ702...
  3. pmc Identification and characterization of the point mutation which affects the transcription level of the chromosomal 3-N-acetyltransferase gene of Streptomyces griseus SS-1198
    J Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 44:437-40. 2000
    ..Therefore, it seemed very likely that the aac(3)-Xa gene was activated by the substitution resulting in the emergence of a stronger promoter...
  4. pmc Contribution of rpoB2 RNA polymerase beta subunit gene to rifampin resistance in Nocardia species
    Jun Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Antimicrob Agents Chemother 50:1342-6. 2006
    ..Since this is the first example of genetic engineering of the Nocardia genome, we believe that this study, as well as our determination of the N. farcinica genome sequence, will be a landmark in Nocardia genetics...
  5. pmc The complete genomic sequence of Nocardia farcinica IFM 10152
    Jun Ishikawa
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162 8640, Japan
    Proc Natl Acad Sci U S A 101:14925-30. 2004
    ..Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease...
  6. pmc Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains
    Keiko Ishino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Clin Microbiol 45:607-9. 2007
    ..aureus. PCR-restriction fragment length polymorphism typing of the coagulase gene was a more reliable method than coagulase serotyping from the viewpoint of arbekacin resistance...
  7. doi request reprint Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance
    Yasutaka Hoshino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    J Antibiot (Tokyo) 63:23-8. 2010
    ..farcinica...
  8. ncbi request reprint Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors
    Kazuhiro Chiba
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Jpn J Infect Dis 60:45-7. 2007
    ..These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp...
  9. pmc Identification of nocobactin NA biosynthetic gene clusters in Nocardia farcinica
    Yasutaka Hoshino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162 8640, Japan
    J Bacteriol 193:441-8. 2011
    ..The ΔnbtE mutant was found to be impaired in cytotoxicity against J774A.1 cells, suggesting that nocobactin NA production is required for virulence of N. farcinica...
  10. ncbi request reprint Trends of arbekacin-resistant MRSA strains in Japanese hospitals (1979 to 2000)
    Naofumi Tsuchizaki
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    J Antibiot (Tokyo) 59:229-33. 2006
    ..If the predominance of type L21 continues, there will be no progression to ABK resistance in MRSA. However, it may be necessary to monitor the trends in type M22 continuously...
  11. doi request reprint Cloning of the gene cluster responsible for the biosynthesis of brasilicardin A, a unique diterpenoid
    Yutaka Hayashi
    Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
    J Antibiot (Tokyo) 61:164-74. 2008
    ..We did not, however, detect any genes related to L-rhamnose and N-acetylglucosamine biosyntheses in the flanking regions. A gene disruption experiment did indeed show that this gene cluster was responsible for BCA biosynthesis...
  12. pmc The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans
    Yuko Sasaki
    Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1, Gakuen, Musashimurayama, Tokyo, Japan
    Nucleic Acids Res 30:5293-300. 2002
    ..Thus, M.penetrans HF-2 may have acquired diverse repertoires of antigenic variation-related genes to allow its persistent infection in humans...
  13. ncbi request reprint Characterization of a bifunctional aminoglycoside-modifying enzyme with novel substrate specificity and its gene from a clinical isolate of methicillin-resistant Staphylococcus aureus with high arbekacin resistance
    Keiko Ishino
    Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Antibiot (Tokyo) 57:679-86. 2004
    ..Therefore, the cloned aac(6')/aph(2") gene may represent the molecular basis for the novel aminoglycoside modification capability as well as novel aminoglycoside resistance profile of S. aureus PRC104...
  14. doi request reprint A Phase I/II study of nilotinib in Japanese patients with imatinib-resistant or -intolerant Ph+ CML or relapsed/refractory Ph+ ALL
    Arinobu Tojo
    The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Int J Hematol 89:679-88. 2009
    ..The results of this study confirmed the efficacy and safety of nilotinib in Japanese patients...
  15. ncbi request reprint Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis
    Haruo Ikeda
    Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228 8555, Japan
    Nat Biotechnol 21:526-31. 2003
    ..In contrast, the terminal regions were not conserved and preferentially contained nonessential genes...
  16. pmc Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350
    Yasuo Ohnishi
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Bacteriol 190:4050-60. 2008
    ..griseus...
  17. doi request reprint YM-341619 suppresses the differentiation of spleen T cells into Th2 cells in vitro, eosinophilia, and airway hyperresponsiveness in rat allergic models
    Keiko Ohga
    Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc, 21 Miyukigaoka, Tsukuba shi, Ibaraki 305 8585, Japan
    Eur J Pharmacol 590:409-16. 2008
    ..These results suggest that YM-341619 has the ability to suppress allergen-induced Th2 responses by selectively inhibiting the differentiation of CD4(+) T cells into the Th2 subset...
  18. doi request reprint An alternative menaquinone biosynthetic pathway operating in microorganisms
    Tomoshige Hiratsuka
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    Science 321:1670-3. 2008
    ..As humans and commensal intestinal bacteria, including lactobacilli, lack this pathway, it represents an attractive target for the development of chemotherapeutics...
  19. ncbi request reprint Characterization of immunoglobulin light chain isotypes in the common carp
    Mitsuru Tomana
    Department of Applied Biological Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
    Immunogenetics 54:120-9. 2002
    ..6-kb transcripts, possibly corresponding to fully spliced (LVJC) and truncated (JC/J-C) forms, respectively...
  20. ncbi request reprint Nocardia terpenica sp. nov., isolated from Japanese patients with nocardiosis
    Yasutaka Hoshino
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
    Int J Syst Evol Microbiol 57:1456-60. 2007
    ..nov. is proposed for the two strains. The type strain is IFM 0706(T) (=JCM 13033(T)=DSM 44935(T)=NBRC 100888(T))...
  21. ncbi request reprint [The genome sequence of soil bacterium Streptomyces]
    Haruo Ikeda
    Tanpakushitsu Kakusan Koso 47:1845-50. 2002
  22. pmc Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism
    Kenji Ueda
    Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
    Nucleic Acids Res 32:4937-44. 2004
    ..The genomic information from S.thermophilum offers new insights into microbial diversity and evolutionary sciences, and provides a framework for characterizing the molecular basis underlying microbial commensalism...
  23. ncbi request reprint Transvalencin Z, a new antimicrobial compound with salicylic acid residue from Nocardia transvalensis IFM 10065
    Akira Mukai
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan
    J Antibiot (Tokyo) 59:366-9. 2006
    ..Transvalencin Z shows a strong antimicrobial activity against Gram-positive bacteria, but shows no activity against Gram-negative bacteria, fungi and tumor cells...
  24. ncbi request reprint A pyrazole derivative, YM-58483, potently inhibits store-operated sustained Ca2+ influx and IL-2 production in T lymphocytes
    Jun Ishikawa
    Inflammation Research, Pharmacology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, Tsukuba shi, Ibaraki, Japan
    J Immunol 170:4441-9. 2003
    ..Furthermore, YM-58483 would be a candidate for the study of capacitative Ca(2+) entry mechanisms through SOC/CRAC channels and for identification of putative Ca(2+) channel genes...
  25. ncbi request reprint Transvalencin A, a thiazolidine zinc complex antibiotic produced by a clinical isolate of Nocardia transvalensis. II. Structure elucidation
    Yasutaka Hoshino
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba, Chiba 260 8673, Japan
    J Antibiot (Tokyo) 57:803-7. 2004
    ..Transvalencin A is comprised of o-substituted p-chlorophenol, tetrasubstituted oxazoline, disubstituted thiazolyl-N-methylthiazolidine and monosubstituted N-methylthiazolidine...
  26. ncbi request reprint Transvalencin A, a thiazolidine zinc complex antibiotic produced by a clinical isolate of Nocardia transvalensis. I. Taxonomy, fermentation, isolation and biological activities
    Yasutaka Hoshino
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1 8 1, Inohana, Chuo Ku, Chiba, Chiba 260 8673, Japan
    J Antibiot (Tokyo) 57:797-802. 2004
    ..The antibiotic is also active against Gram-positive bacteria such as Micrococcus luteus. We observed higher activity for fungi in an acidic medium than in a neutral medium...
  27. ncbi request reprint Characterisation of a fourth immunoglobulin light chain isotype in the common carp
    Jun Ishikawa
    Department of Applied Biological Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
    Fish Shellfish Immunol 16:369-79. 2004
    ..Southern blot analyses suggested that the locus of carp L2 has a cluster-like organisation. Phylogenetic analyses showed that both carp VL2 and CL2 amino acid sequences highly clustered with other teleost L2 sequences...
  28. ncbi request reprint Characterisation of T cell antigen receptor alpha chain isotypes in the common carp
    Etsuou Imai
    Department of Applied Biological Science, Nihon University, Fujisawa, Kanagawa, Japan
    Fish Shellfish Immunol 19:205-16. 2005
    ..Therefore, the results obtained using an individual clearly showed that carp possesses at least two Calpha loci, possibly as a result of tetraploidisation...