Yuzuru Ikehara

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. ncbi request reprint Effective induction of anti-tumor immune responses with oligomannose-coated liposome targeting to intraperitoneal phagocytic cells
    Yuzuru Ikehara
    Molecular Medicine Team of Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Centeral 2 12, Room 211, 1 1 1 Umezono, Tsukuba, Japan
    Cancer Lett 260:137-45. 2008
  2. pmc Recent advancements in cytotoxic T lymphocyte generation methods using carbohydrate-coated liposomes
    Yuzuru Ikehara
    Molecular Medicine Team of Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8568, Japan
    J Biomed Biotechnol 2010:242539. 2010
  3. doi request reprint Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma
    Atsushi Matsuda
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    J Proteomics 85:1-11. 2013
  4. doi request reprint Beta3GnT2 (B3GNT2), a major polylactosamine synthase: analysis of B3GNT2-deficient mice
    Akira Togayachi
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki, Japan
    Methods Enzymol 479:185-204. 2010
  5. pmc Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation
    Akira Togayachi
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Proc Natl Acad Sci U S A 107:11900-5. 2010
  6. doi request reprint Glycoproteomic discovery of serological biomarker candidates for HCV/HBV infection-associated liver fibrosis and hepatocellular carcinoma
    Hiroyuki Kaji
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Proteome Res 12:2630-40. 2013
  7. doi request reprint Multilectin assay for detecting fibrosis-specific glyco-alteration by means of lectin microarray
    Atsushi Kuno
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Clin Chem 57:48-56. 2011
  8. doi request reprint LecT-Hepa: A triplex lectin-antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside clinical chemistry analyzer and an automated pretreatment machine
    Atsushi Kuno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Clin Chim Acta 412:1767-72. 2011
  9. pmc A serum "sweet-doughnut" protein facilitates fibrosis evaluation and therapy assessment in patients with viral hepatitis
    Atsushi Kuno
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Japan
    Sci Rep 3:1065. 2013
  10. doi request reprint Application of a glycoproteomics-based biomarker development method: alteration in glycan structure on colony stimulating factor 1 receptor as a possible glycobiomarker candidate for evaluation of liver cirrhosis
    Makoto Ocho
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Proteome Res 13:1428-37. 2014

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Effective induction of anti-tumor immune responses with oligomannose-coated liposome targeting to intraperitoneal phagocytic cells
    Yuzuru Ikehara
    Molecular Medicine Team of Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Centeral 2 12, Room 211, 1 1 1 Umezono, Tsukuba, Japan
    Cancer Lett 260:137-45. 2008
    ..G7-OVA (OVA-transfected EL4) but not EL4. These results indicate that the OMLs can also be used as an effective antigen delivery system for cancer immunotherapy activating both CTL and Th subsets...
  2. pmc Recent advancements in cytotoxic T lymphocyte generation methods using carbohydrate-coated liposomes
    Yuzuru Ikehara
    Molecular Medicine Team of Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8568, Japan
    J Biomed Biotechnol 2010:242539. 2010
    ....
  3. doi request reprint Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma
    Atsushi Matsuda
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    J Proteomics 85:1-11. 2013
    ..84, AUC=0.93). This combination will possibly be a precise procedure for CC diagnosis compared with conventional diagnostic techniques...
  4. doi request reprint Beta3GnT2 (B3GNT2), a major polylactosamine synthase: analysis of B3GNT2-deficient mice
    Akira Togayachi
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki, Japan
    Methods Enzymol 479:185-204. 2010
    ..This finding indicates that polylactosamine has an important role in immunological biofunctions. We can therefore attempt to identify the in vivo biological function of glycans using glycogene-deficient mice...
  5. pmc Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation
    Akira Togayachi
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Proc Natl Acad Sci U S A 107:11900-5. 2010
    ..Together, these results suggest that lacto/neolacto-series GSLs play an important role in clustering of GEMs and tether-specific proteins, such as BCR, CD19, and related signaling molecules to the GEMs...
  6. doi request reprint Glycoproteomic discovery of serological biomarker candidates for HCV/HBV infection-associated liver fibrosis and hepatocellular carcinoma
    Hiroyuki Kaji
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Proteome Res 12:2630-40. 2013
    ..These results indicate that our glycoproteomic strategy is effective for identifying multiple glyco-biomarker candidates in a high-throughput manner...
  7. doi request reprint Multilectin assay for detecting fibrosis-specific glyco-alteration by means of lectin microarray
    Atsushi Kuno
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Clin Chem 57:48-56. 2011
    ..We therefore developed a compatible multiple lectin-antibody sandwich immunoassay on the basis of the results obtained by the lectin microarray analysis for monitoring fibrosis...
  8. doi request reprint LecT-Hepa: A triplex lectin-antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside clinical chemistry analyzer and an automated pretreatment machine
    Atsushi Kuno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Clin Chim Acta 412:1767-72. 2011
    ..In the analysis of glycans, a manual pretreatment process for clinical specimens leads to a complicated manipulation and loss-of-clinical implementation of the assay...
  9. pmc A serum "sweet-doughnut" protein facilitates fibrosis evaluation and therapy assessment in patients with viral hepatitis
    Atsushi Kuno
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Japan
    Sci Rep 3:1065. 2013
    ..FastLec-Hepa is the only assay currently available for clinically beneficial therapy evaluation through quantitation of disease severity...
  10. doi request reprint Application of a glycoproteomics-based biomarker development method: alteration in glycan structure on colony stimulating factor 1 receptor as a possible glycobiomarker candidate for evaluation of liver cirrhosis
    Makoto Ocho
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Proteome Res 13:1428-37. 2014
    ..3 × 10(-17)). This result suggests WFA(+)-CSF1R is a possible biomarker candidate for evaluation of LC. Our results verified feasibility of this strategy for glycobiomarker development. ..
  11. pmc Podocalyxin is a glycoprotein ligand of the human pluripotent stem cell-specific probe rBC2LCN
    Hiroaki Tateno
    Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Stem Cells Transl Med 2:265-73. 2013
    ..5 × 10(4) M(-1)) prepared from human 201B7 iPS cells, indicating that H type 3 is a most probable potential pluripotency marker. We conclude that podocalyxin is a glycoprotein ligand of rBC2LCN on human iPS cells and ES cells...
  12. pmc Enhancement of metastatic ability by ectopic expression of ST6GalNAcI on a gastric cancer cell line in a mouse model
    Hidenori Ozaki
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Clin Exp Metastasis 29:229-38. 2012
    ..Identification of sTn carrier proteins will help understand mechanisms of metastatic phenotype acquisition of gastric cancer cells by ST6GalNAcI and sTn...
  13. doi request reprint Differential glycan profiling by lectin microarray targeting tissue specimens
    Atsushi Kuno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Central 2, Umezono, Ibaraki, Japan
    Methods Enzymol 478:165-79. 2010
    ....
  14. doi request reprint Novel glycobiomarker for ovarian cancer that detects clear cell carcinoma
    Maki Sogabe
    Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Central 2, 1 1 1 Umezono, Tsukuba 305 8568, Japan
    J Proteome Res 13:1624-35. 2014
    ..Thus, our glycoproteomic strategy featuring efficient enrichment of glycans with disease-related alterations is applicable to various diseases. ..
  15. doi request reprint Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma
    Atsushi Matsuda
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 2, Tsukuba, Ibaraki, Japan
    Hepatology 52:174-82. 2010
    ..0%, specificity = 76.3%, and area under the curve = 0.85). As a particular note, the obtained sensitivity is the highest score among those having been so far reported...
  16. pmc Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism
    Takashi Sato
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory Central 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    J Biol Chem 286:5803-12. 2011
    ..Our observations suggest that CSGalNAcT-1 is necessary for normal levels of endochondral ossification, and the decrease in CS amount in the growth plate by its absence causes a rapid catabolism of aggrecan...
  17. doi request reprint Generation of IFN-gamma-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines
    Takashi Yamaguchi
    Molecular Medicine Team of Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Vet Parasitol 171:207-15. 2010
    ..orientalis-specific T cells in T. orientalis-infected bovines using an antigen-specific ELISpot assay system and that T. orientalis-specific, IFN-gamma- and IL-10-producing T cells are produced in T. orientalis-infected Holsteins...
  18. doi request reprint A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics
    Hisashi Narimatsu
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
    FEBS J 277:95-105. 2010
    ..These candidate glycoproteins were immunoprecipitated from serum using commercially available antibodies, and their glycan alteration was examined by a lectin microarray. Finally, they were analyzed by multistage tandem MS...
  19. doi request reprint C1galt1-deficient mice exhibit thrombocytopenia due to abnormal terminal differentiation of megakaryocytes
    Takashi Kudo
    Department of Anatomy and Embryology, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan
    Blood 122:1649-57. 2013
    ..Our observations suggest that O-glycan is required for terminal megakaryocyte differentiation and platelet production and that the decrease in GPIbα in cells lacking O-glycan might be caused by increased proteolysis. ..
  20. doi request reprint Strategy for glycoproteomics: identification of glyco-alteration using multiple glycan profiling tools
    Hiromi Ito
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    J Proteome Res 8:1358-67. 2009
    ..Using our system, we successfully identified glycan alterations on alpha-fetoprotein (AFP), including a novel LacdiNAc structure in addition to previously reported alterations such as alpha1,6 fucosylation...
  21. doi request reprint Comparison of LecT-Hepa and FibroScan for assessment of liver fibrosis in hepatitis B virus infected patients with different ALT levels
    Dongning Du
    Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Er Road, Shanghai 200025, China
    Clin Chim Acta 413:1796-9. 2012
    ..We compared the reliability of LecT-Hepa with that of FibroScan for evaluation of liver fibrosis...
  22. ncbi request reprint Development of a novel oligomannose-coated liposome-based anticancer drug-delivery system for intraperitoneal cancer
    Yuzuru Ikehara
    Research Center for Glycoscience, National Institute of Advanced Industrial Technology, 1 1 1 Umezono, Tsukuba shi, Ibaraki 305 8568, Japan
    Curr Opin Mol Ther 9:53-61. 2007
    ....
  23. doi request reprint Immunoassay with single-walled carbon nanotubes as near-infrared fluorescent labels
    Yoko Iizumi
    Nanotube Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, 305 8565, Japan
    ACS Appl Mater Interfaces 5:7665-70. 2013
    ..The photoluminescence intensity of the CNT labels was strong enough to detect antigens at 600 pM by our simple procedures. ..
  24. doi request reprint Human C21orf63 is a heparin-binding protein
    Kanae Mitsunaga
    Glycogene Function Team, Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    J Biochem 146:369-73. 2009
    ..Results of real-time polymerase chain reaction and tissue immunostaining imply that C21orf63 is expressed on epithelia of various human tissues...
  25. ncbi request reprint Immunocytochemical analysis for intracellular dynamics of C1GalT associated with molecular chaperone, Cosmc
    Yoshiki Narimatsu
    Glycogene Function Team of Research Center for Medical Glycoscience RCMG, National Institute of Advanced Industrial Science and Technology AIST, Open Space Laboratory Central 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Biochem Biophys Res Commun 366:199-205. 2008
    ..These results suggest that the intracellular dynamics of C1GalT is controlled by its specific molecular chaperon, Cosmc, in association with core 1 synthase activity...
  26. pmc Polylactosamine on glycoproteins influences basal levels of lymphocyte and macrophage activation
    Akira Togayachi
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Proc Natl Acad Sci U S A 104:15829-34. 2007
    ..These results indicate that polylactosamine on N-glycans is a putative immune regulatory factor presumably suppressing excessive responses during immune reactions...
  27. ncbi request reprint Apical Golgi localization of N,N'-diacetyllactosediamine synthase, beta4GalNAc-T3, is responsible for LacdiNAc expression on gastric mucosa
    Yuzuru Ikehara
    Glycostructure Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory Central 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Glycobiology 16:777-85. 2006
    ....
  28. doi request reprint Single-walled carbon nanohorns as drug carriers: adsorption of prednisolone and anti-inflammatory effects on arthritis
    Maki Nakamura
    National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba 305 8565, Japan
    Nanotechnology 22:465102. 2011
    ..By histological analysis of the ankle joint, the anti-inflammatory effect of PSL-oxSWNHs was also observed...
  29. ncbi request reprint Mice lacking alpha1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors
    Takashi Kudo
    Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Central 2, Open Space Laboratory, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Glycobiology 17:1-9. 2007
    ....
  30. ncbi request reprint A carbohydrate recognition-based drug delivery and controlled release system using intraperitoneal macrophages as a cellular vehicle
    Yuzuru Ikehara
    Division of Oncological Pathology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Cancer Res 66:8740-8. 2006
    ..Thus, we have established the use of i.p. macrophages as a novel drug delivery system for the control of cancer metastatic to milky spots...
  31. ncbi request reprint Oligomannose-coated liposomes as a therapeutic antigen-delivery and an adjuvant vehicle for induction of in vivo tumor immunity
    Naoya Kojima
    Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa 259 1292, Japan
    J Control Release 129:26-32. 2008
    ..These results indicate that an OML-based vaccine with an encased tumor antigen might be useful clinically to raise an effective immune response against a tumor...
  32. ncbi request reprint Establishment and characterization of a human colonic mucinous carcinoma cell line with predominant goblet-cell differentiation from liver metastasis
    Takasuke Yamachika
    Division of Oncological Pathology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Pathol Int 55:550-7. 2005
    ....
  33. ncbi request reprint Effect of CD4+CD25+ T cell-depletion on acute lethal infection of mice with Trypanosoma congolense
    Boniface Namangala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    J Vet Med Sci 70:751-9. 2008
    ..Cumulatively, these findings suggest that CD4+CD25+ T-cell- depletion improves the trypanotolerance of highly susceptible BALB/c mice acutely infected with the lethal T. congolense...
  34. ncbi request reprint Negative regulation of T cell receptor signaling by Siglec-7 (p70/AIRM) and Siglec-9
    Yuzuru Ikehara
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:43117-25. 2004
    ..The combined results demonstrate that both Siglec-7 and Siglec-9 are capable of negative regulation of TCR signaling and that ligand binding is required for optimal activity...
  35. ncbi request reprint Lewis type 1 antigen synthase (beta3Gal-T5) is transcriptionally regulated by homeoproteins
    Soichiro Isshiki
    Division of Cell Biology, Institute of Life Science, Soka University, Tangi cho, Hachioji, Tokyo 192 8577, Japan
    J Biol Chem 278:36611-20. 2003
    ..beta3Gal-T5, controlled by the intestinal homeoproteins, may play an important role in the specific function of intestinal cells by modifying the carbohydrate structure of glycoproteins...