Kentaro Hanada

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. ncbi Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes
    Satoko Saito
    Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    J Biol Chem 283:6584-93. 2008
  2. ncbi A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis
    K Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 Japan
    J Biol Chem 272:32108-14. 1997
  3. ncbi Molecular machinery for non-vesicular trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Nature 426:803-9. 2003
  4. ncbi Serine palmitoyltransferase, a key enzyme of sphingolipid metabolism
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Biochim Biophys Acta 1632:16-30. 2003
  5. ncbi CERT-mediated trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biochim Biophys Acta 1791:684-91. 2009
  6. ncbi [Cultured cell mutants defective in synthesis of membrane lipids: a key tool for studies on membrane lipid domains]
    Kentaro Hanada
    Tanpakushitsu Kakusan Koso 47:425-30. 2002
  7. ncbi [Biosynthesis of sphingolipids]
    Kentaro Hanada
    Tanpakushitsu Kakusan Koso 47:403-8. 2002
  8. ncbi Sphingolipids in infectious diseases
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Jpn J Infect Dis 58:131-48. 2005
  9. ncbi CERT and intracellular trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, 162 8640, Japan
    Biochim Biophys Acta 1771:644-53. 2007
  10. ncbi Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase
    K Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Biol Chem 273:33787-94. 1998

Detail Information

Publications70

  1. ncbi Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes
    Satoko Saito
    Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    J Biol Chem 283:6584-93. 2008
    ..These results suggest that CERT is a physiological substrate of PP2Cepsilon and that dephosphorylation of CERT by PP2Cepsilon may play an important role in the regulation of ceramide trafficking from the ER to the Golgi apparatus...
  2. ncbi A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis
    K Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 Japan
    J Biol Chem 272:32108-14. 1997
    ..These results indicate that the CHO LCB1 homolog encodes a component of SPT...
  3. ncbi Molecular machinery for non-vesicular trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Nature 426:803-9. 2003
    ..We conclude that CERT mediates the intracellular trafficking of ceramide in a non-vesicular manner...
  4. ncbi Serine palmitoyltransferase, a key enzyme of sphingolipid metabolism
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku, Tokyo 162 8640, Japan
    Biochim Biophys Acta 1632:16-30. 2003
    ..Specific missense mutations in the human LCB1 gene cause hereditary sensory neuropathy type I, an autosomal dominantly inherited disease, and these mutations confer dominant-negative effects on SPT activity...
  5. ncbi CERT-mediated trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biochim Biophys Acta 1791:684-91. 2009
    ....
  6. ncbi [Cultured cell mutants defective in synthesis of membrane lipids: a key tool for studies on membrane lipid domains]
    Kentaro Hanada
    Tanpakushitsu Kakusan Koso 47:425-30. 2002
  7. ncbi [Biosynthesis of sphingolipids]
    Kentaro Hanada
    Tanpakushitsu Kakusan Koso 47:403-8. 2002
  8. ncbi Sphingolipids in infectious diseases
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Jpn J Infect Dis 58:131-48. 2005
    ..This review summarizes recent advances in sphingolipid biology related to infectious diseases...
  9. ncbi CERT and intracellular trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, 162 8640, Japan
    Biochim Biophys Acta 1771:644-53. 2007
    ....
  10. ncbi Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase
    K Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Biol Chem 273:33787-94. 1998
    ..Moreover, these results demonstrate that the SPT enzyme comprises both the LCB1 and LCB2 proteins...
  11. ncbi Neutral sphingomyelinase activity dependent on Mg2+ and anionic phospholipids in the intraerythrocytic malaria parasite Plasmodium falciparum
    K Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biochem J 346:671-7. 2000
    ..Our finding that the malaria parasites possess SMase activity might explain why the parasites seem to have an SM synthase activity but no activity to synthesize ceramide de novo...
  12. ncbi Specificity of inhibitors of serine palmitoyltransferase (SPT), a key enzyme in sphingolipid biosynthesis, in intact cells. A novel evaluation system using an SPT-defective mammalian cell mutant
    K Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    Biochem Pharmacol 59:1211-6. 2000
    ..Thus, the cytotoxicity of sphingofungin B and ISP-1, but not L-cycloserine or beta-chloro-L-alanine, is due largely to inhibition of sphingolipid synthesis by inhibiting the SPT activity...
  13. ncbi Plasmodium falciparum phospholipase C hydrolyzing sphingomyelin and lysocholinephospholipids is a possible target for malaria chemotherapy
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, Japan Science and Technology Corporation, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Exp Med 195:23-34. 2002
    ....
  14. ncbi D-Serine inhibits serine palmitoyltransferase, the enzyme catalyzing the initial step of sphingolipid biosynthesis
    K Hanada
    Department of Biochemistry, National Institute of Infectious Diseases, 1 23 1, Toyama, Tokyo, Japan
    FEBS Lett 474:63-5. 2000
    ..These results suggest that D-serine competes with L-serine for the amino acid recognition site of SPT, but that D-serine is not utilized by this enzyme to produce KDS...
  15. ncbi Casein kinase I{gamma}2 down-regulates trafficking of ceramide in the synthesis of sphingomyelin
    Nario Tomishige
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    Mol Biol Cell 20:348-57. 2009
    ..These results indicate that CKIgamma2 hyperphosphorylates the serine-repeat motif of CERT, thereby inactivating CERT and down-regulating the synthesis of sphingomyelin...
  16. ncbi Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT
    Miyuki Kawano
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 281:30279-88. 2006
    ..These results suggest that the Golgi-targeting PH domain and ER-interacting FFAT motif of CERT spatially restrict the random ceramide transfer activity of the START domain in cells...
  17. ncbi Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Shinjyuku, Tokyo, Japan
    Autophagy 5:937-45. 2009
    ....
  18. ncbi Interorganelle trafficking of ceramide is regulated by phosphorylation-dependent cooperativity between the PH and START domains of CERT
    Keigo Kumagai
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 282:17758-66. 2007
    ..The cooperative control of functionally distinct domains of CERT is a novel molecular event to regulate the intracellular trafficking of ceramide...
  19. ncbi [The molecular machinery CERT for intracellular trafficking of ceramide]
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
    Seikagaku 76:562-70. 2004
  20. ncbi Mouse prion protein (PrP) segment 100 to 104 regulates conversion of PrP(C) to PrP(Sc) in prion-infected neuroblastoma cells
    Hideyuki Hara
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    J Virol 86:5626-36. 2012
    ..These results suggest the segment 100 to 104 of MoPrP(C) plays a key role in conversion after binding to MoPrP(Sc)...
  21. ncbi [New paradigm of membrane transport: ER-to-Golgi trafficking of the lipid ceramide by a molecular extraction and transfer mechanism]
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases
    Nippon Saikingaku Zasshi 60:531-7. 2005
  22. ncbi Two sphingolipid transfer proteins, CERT and FAPP2: their roles in sphingolipid metabolism
    Toshiyuki Yamaji
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
    IUBMB Life 60:511-8. 2008
    ..These observations indicate that lipid transfer proteins, CERT and FAPP2, spatially regulate lipid metabolism on the cytosolic side...
  23. ncbi CERT mediates intermembrane transfer of various molecular species of ceramides
    Keigo Kumagai
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640 and Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 280:6488-95. 2005
    ..These results indicate that CERT can mediate transfer of various types of ceramides that naturally exist and their close relatives...
  24. ncbi Reconstitution assay system for ceramide transport with semi-intact cells
    Keigo Kumagai
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Methods Cell Biol 108:117-29. 2012
    ..This chapter describes a reconstitution assay system for ceramide transport with semi-intact cells, which is useful for the study of the CERT-mediated inter-organelle transport of ceramide...
  25. ncbi Critical role of virion-associated cholesterol and sphingolipid in hepatitis C virus infection
    Hideki Aizaki
    Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Virol 82:5715-24. 2008
    ....
  26. ncbi Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells
    Yuko Nitahara-Kasahara
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo, Japan
    Virology 383:319-27. 2009
    ..HCV production was also affected by cellular vimentin content. Our findings together suggest that modulation of hepatic vimentin expression might enable the control of HCV production...
  27. ncbi Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT)
    Toshihiko Sugiki
    Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
    J Biol Chem 287:33706-18. 2012
    ....
  28. ncbi Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases
    Yukiko Miura
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    FEBS Lett 557:288-92. 2004
    ....
  29. ncbi An improved method for cell-to-cell transmission of infectious prion
    Masahiko Tanaka
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biochem Biophys Res Commun 397:505-8. 2010
    ..Further characterization showed that higher cell density greatly facilitated the transmission of PrP(Sc). This improved in vitro transmission method may become a useful tool for unveiling the molecular mechanism of PrP(Sc) transmission...
  30. ncbi The preparation of a lipidic endotoxin affects its biological activities
    Kazuo Okemoto
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Biol Pharm Bull 31:1952-4. 2008
    ....
  31. ncbi Discovery of the molecular machinery CERT for endoplasmic reticulum-to-Golgi trafficking of ceramide
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Tokyo 162 8640, Japan
    Mol Cell Biochem 286:23-31. 2006
    ..In this short review, I summarise recent advances in molecular mechanisms of intracellular transport of ceramide, focusing on our genetic and biochemical approaches to this issue...
  32. ncbi Purification of mammalian serine palmitoyltransferase, a hetero-subunit enzyme for sphingolipid biosynthesis, by affinity-peptide chromatography
    Kentaro Hanada
    Department of Biochemsitry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    Methods Mol Biol 228:163-74. 2003
  33. ncbi Transmembrane BAX inhibitor motif containing (TMBIM) family proteins perturbs a trans-Golgi network enzyme, Gb3 synthase, and reduces Gb3 biosynthesis
    Toshiyuki Yamaji
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 285:35505-18. 2010
    ..Furthermore, GRINA-C was associated with Gb3 synthase. These observations may demonstrate a new type of posttranscriptional regulation of glycosyltransferases...
  34. ncbi Localization, topology, and function of the LCB1 subunit of serine palmitoyltransferase in mammalian cells
    Satoshi Yasuda
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 278:4176-83. 2003
    ....
  35. ncbi Identification and structural analysis of C-terminally truncated collapsin response mediator protein-2 in a murine model of prion diseases
    Fumiko Shinkai-Ouchi
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Proteome Sci 8:53. 2010
    ..However, the underlying molecular basis for these neuropathogenic abnormalities is not fully understood...
  36. ncbi RalA functions as an indispensable signal mediator for the nutrient-sensing system
    Tomohiko Maehama
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Biol Chem 283:35053-9. 2008
    ..These results collectively suggest that RalGDS and RalA act downstream of Rheb and that RalA activation is a crucial step in nutrient-induced mTORC1 activation...
  37. ncbi [Acquired human prion diseases--past and present issues]
    Ken ichi Hagiwara
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Uirusu 59:155-65. 2009
    ..To date, five instances of probable transfusion-mediated transmission of vCJD prion have been found in UK. Here we review the past and the present issues about the acquired human prion diseases...
  38. ncbi Intracellular trafficking of ceramide by ceramide transfer protein
    Kentaro Hanada
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:426-37. 2010
    ....
  39. ncbi Synthetic fibril peptide promotes clearance of scrapie prion protein by lysosomal degradation
    Yuko Okemoto-Nakamura
    Department of Biochemistry and Cell Biology, National Institue of Infectious Diseases, Tokyo, Japan
    Microbiol Immunol 52:357-65. 2008
    ..These results indicate that P9 promotes PrP(Sc) redistribution from late endosomes to lysosomes, thereby attaining PrP(Sc) degradation...
  40. ncbi [Lipid raft domains in sphingolipid-deficient mutants]
    Masayoshi Fukasawa
    Tanpakushitsu Kakusan Koso 47:321-5. 2002
  41. ncbi A potent adjuvant monophosphoryl lipid A triggers various immune responses, but not secretion of IL-1beta or activation of caspase-1
    Kazuo Okemoto
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    J Immunol 176:1203-8. 2006
    ..These results suggest that an immune response without activation of caspase-1 or secretion of IL-1beta results in the low toxicity of this adjuvant...
  42. ncbi Enhancement of de novo fatty acid biosynthesis in hepatic cell line Huh7 expressing hepatitis C virus core protein
    Masayoshi Fukasawa
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
    Biol Pharm Bull 29:1958-61. 2006
    ..Up-regulation of de novo fatty acid biosynthesis by HCV core protein may affect cellular lipid metabolism, resulting in neutral lipid accumulation in HCV-infected cells...
  43. ncbi Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Disease, Tokyo, Japan
    Autophagy 4:131-4. 2008
    ..These mutant LC3DeltaG proteins will be useful negative controls in recognizing non-specific fluorescent protein-LC3 aggregates...
  44. ncbi Initial steps of Shigella infection depend on the cholesterol/sphingolipid raft-mediated CD44-IpaB interaction
    Frank Lafont
    Department of Genetics and Microbiology, Centre Medical Universitaire, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    EMBO J 21:4449-57. 2002
    ..Our results show that rafts are implicated in Shigella binding and entry, suggesting that raft-associated molecular machineries are engaged in mediating the cell signalling response required for the invasion process...
  45. ncbi Hereditary sensory neuropathy type 1 mutations confer dominant negative effects on serine palmitoyltransferase, critical for sphingolipid synthesis
    Khemissa Bejaoui
    Day Neuromuscular Research Laboratory, Charlestown, Massachusetts, USA
    J Clin Invest 110:1301-8. 2002
    ..These results indicate that the HSN1-associated mutations in LCB1 confer dominant negative effects on the SPT enzyme...
  46. ncbi Evidence that clustered phosphocholine head groups serve as sites for binding and assembly of an oligomeric protein pore
    Angela Valeva
    Institute of Medical Microbiology and Hygiene, Hochhaus am Augustusplatz, D-55101 Mainz, Germany
    J Biol Chem 281:26014-21. 2006
    ..The principle of membrane targeting in the absence of any genuine, high affinity receptor may also underlie the assembly of other lipid-inserted oligomers including cytotoxic peptides, protein toxins, and immune effector molecules...
  47. ncbi A role for the de novo sphingolipids in apoptosis of photosensitized cells
    Bambang Wispriyono
    Department of Occupational and Environmental Health Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Detroit Michigan 48201, USA
    Exp Cell Res 279:153-65. 2002
    ..Hence, the data are consistent with the partial involvement of the de novo sphingolipid pathway in apoptosis via DEVDase activation downstream of mitochondrial cytochrome c release post-Pc 4-PDT...
  48. ncbi [Sphingolipid biosynthesis and membrane traffic]
    Yoshio Hirabayashi
    Tanpakushitsu Kakusan Koso 47:400-2. 2002
  49. ncbi Decreased ceramide transport protein (CERT) function alters sphingomyelin production following UVB irradiation
    Alexandra Charruyer
    Department of Dermatology, School of Medicine, University of California, Northern California Institute for Research and Education, and Veterans Affairs Medical Center, San Francisco, California 94121, USA
    J Biol Chem 283:16682-92. 2008
    ....
  50. ncbi Functional analysis of alpha5beta1 integrin and lipid rafts in invasion of epithelial cells by Porphyromonas gingivalis using fluorescent beads coated with bacterial membrane vesicles
    Kayoko Tsuda
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka, Japan
    Cell Struct Funct 33:123-32. 2008
    ..gingivalis adhesion to epithelial cells, while the subsequent uptake process requires lipid raft components for actin organization, with Rho GTPase Rac1...
  51. ncbi Stereoselective synthesis and structure-activity relationship of novel ceramide trafficking inhibitors. (1R,3R)-N-(3-hydroxy-1-hydroxymethyl-3-phenylpropyl)dodecanamide and its analogues
    Yoshitaka Nakamura
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyou-ku, Tokyo 113-0033, Japan
    J Med Chem 46:3688-95. 2003
    ..Installation of another hydroxy group onto HPA-12 at the same position as that in the natural ceramide was also conducted, but no enhancement of the activity was observed...
  52. ncbi Enhanced apoA-I-dependent cholesterol efflux by ABCA1 from sphingomyelin-deficient Chinese hamster ovary cells
    Kohjiro Nagao
    Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Kyoto University Graduate School of Agriculture, Kyoto 606 8502, Japan
    J Biol Chem 282:14868-74. 2007
    ....
  53. ncbi Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements
    Zhi-Jie Cheng
    Department of Biochemistry and Molecular Biology, Thoracic Diseases Research Unit, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Mol Biol Cell 17:3197-210. 2006
    ..These results provide the first evidence that SLs are differentially required for distinct mechanisms of clathrin-independent endocytosis...
  54. ncbi Diphtheria toxin translocation across cellular membranes is regulated by sphingolipids
    Bjørn Spilsberg
    Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Biochem Biophys Res Commun 329:465-73. 2005
    ..In conclusion, diphtheria toxin translocation is facilitated by depletion of membrane sphingolipids...
  55. ncbi De novo ceramide accumulation due to inhibition of its conversion to complex sphingolipids in apoptotic photosensitized cells
    Vladislav Dolgachev
    Occupational and Environmental Health Sciences, The Department of Fundamental and Applied Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Detroit, Michigan 48201, USA
    J Biol Chem 279:23238-49. 2004
    ..These results are the first to show that in the absence of SPT up-regulation PDT induces accumulation of de novo ceramide by inhibiting its conversion to complex sphingolipids...
  56. ncbi Ceramide traffic in C6 glioma cells: evidence for CERT-dependent and independent transport from ER to the Golgi apparatus
    Paola Giussani
    Department of Medical Chemistry, Biochemistry and Biotechnology, L I T A via F lli Cervi 93, 20090 Segrate, Milan, Italy
    Biochim Biophys Acta 1781:40-51. 2008
    ....
  57. ncbi Sphingomyelin-dependence of cholesterol efflux mediated by ABCG1
    Osamu Sano
    Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Kyoto University Graduate School of Agriculture, Kyoto, Japan
    J Lipid Res 48:2377-84. 2007
    ..These results suggest that the ABCG1-mediated efflux of cholesterol and SM is dependent on the cellular SM level and distribution of cholesterol in the plasma membrane...
  58. ncbi Differential effects of modification of membrane cholesterol and sphingolipids on the conformation, function, and trafficking of the G protein-coupled cholecystokinin receptor
    Kaleeckal G Harikumar
    Mayo Clinic Scottsdale, Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Scottsdale, Arizona 85259, USA
    J Biol Chem 280:2176-85. 2005
    ..Thus, lipid microenvironment of the CCK receptor is particularly important, with different lipids having distinct effects...
  59. ncbi Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide
    Norio Kudo
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
    Proc Natl Acad Sci U S A 105:488-93. 2008
    ..Thus, the structures demonstrate the structural basis for the mechanism by which CERT can distinguish ceramide from other lipid types yet still recognize multiple species of ceramides...
  60. ncbi Depletion of sphingolipids facilitates endosome to Golgi transport of ricin
    Stine Grimmer
    Institute for Cancer Research, Department of Biochemistry, The Norwegian Radium Hospital, University of Oslo, Montebello, 0310 Oslo, Norway
    Traffic 7:1243-53. 2006
    ..Our results indicate that the presence of sphingolipids actually limits and may function to control endosome to Golgi transport of ricin...
  61. ncbi Ceramide response post-photodamage is absent after treatment with HA14-1
    Duska Separovic
    Department of Fundamental and Applied Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA
    Biochem Biophys Res Commun 345:803-8. 2006
    ..These results show that the ceramide response to PDT is not induced by another pro-apoptotic stimulus, and may be unique to PDT as described here...
  62. ncbi Ceramide sensitizes astrocytes to oxidative stress: protective role of cannabinoids
    Arkaitz Carracedo
    Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, 28040 Madrid, Spain
    Biochem J 380:435-40. 2004
    ....
  63. ncbi Modulation of amyloid precursor protein cleavage by cellular sphingolipids
    Naoya Sawamura
    Department of Dementia Research, National Institute for Longevity Sciences, 36-3 Gengo, Morioka, Obu, Aichi 474-8522, Japan
    J Biol Chem 279:11984-91. 2004
    ..These results suggest that not only the cellular level of cholesterol but also that of sphingolipids may be involved in the pathological process of Alzheimer's disease by modulating APP cleavage...
  64. ncbi Suppression of sphingomyelin synthase 1 by small interference RNA is associated with enhanced ceramide production and apoptosis after photodamage
    Duska Separovic
    Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA
    Exp Cell Res 314:1860-8. 2008
    ..These findings suggest that SMS is a potential novel molecular target that can augment therapeutic efficacy of PDT...
  65. ncbi Chemistry and biology of khafrefungin. Large-scale synthesis, design, and structure-activity relationship of khafrefungin, an antifungal agent
    Masayuki Nakamura
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Org Biomol Chem 1:3362-76. 2003
    ..A macrocyclic khafrefungin derivative was also synthesized, but the antifungal activity was lost. These results suggest that the structure of khafrefungin might be strictly recognized in fungi...
  66. ncbi Sphingomyelin synthase 1 suppresses ceramide production and apoptosis post-photodamage
    Duska Separovic
    Department of Fundamental and Applied Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA
    Biochem Biophys Res Commun 358:196-202. 2007
    ..The data show that overexpression of SMS1 is associated with suppressed ceramide response and apoptotic resistance after photodamage...
  67. ncbi Protein-sphingolipid interactions within cellular membranes
    Per Haberkant
    Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany
    J Lipid Res 49:251-62. 2008
    ..This novel photolabile sphingolipid probe allows the detection of protein-sphingolipid interactions within the membrane bilayer of living cells...
  68. ncbi Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in Arabidopsis
    Lihua Shi
    National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China
    Cell Res 17:1030-40. 2007
    ....
  69. ncbi Reactive oxygen species generation is independent of de novo sphingolipids in apoptotic photosensitized cells
    Vladislav Dolgachev
    Department of Occupational and Environmental Health Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Detroit, MI 48201, USA
    Exp Cell Res 288:425-36. 2003
    ....
  70. ncbi Accumulation of diacylglycerol in the Chlamydia inclusion vacuole: possible role in the inhibition of host cell apoptosis
    Shirley M L Tse
    Department of Pediatrics, Division of Rheumatology, The Hospital for Sick Children, University of Toronto, Toronto M5G 1X8, Canada
    J Biol Chem 280:25210-5. 2005
    ..The scavenging of pro-apoptotic factors may provide a novel mechanism whereby pathogens promote their own survival by extending the life of the host cells they infect...