Research Topics
| Petr GruzSummaryAffiliation: National Institute of Health Sciences Country: Japan Publications
| Collaborators
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Detail Information
Publications
Processing of DNA lesions by archaeal DNA polymerases from Sulfolobus solfataricusPetr Gruz
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
Nucleic Acids Res 31:4024-30. 2003..Additionally, we reveal that the deaminated bases can be introduced into DNA enzymatically, since both PolB1 and PolY1 are able to incorporate the aberrant DNA precursors dUTP and dITP...
Origins of age-related DNA damage and dietary strategies for its reductionPetr Gruz
Division of Genetic and Mutagenesis II, National Institute of Health Sciences, Tokyo, Japan
Rejuvenation Res 13:285-7. 2010..This may be particularly beneficial to the aging organism, which has progressively impaired natural protective systems...
Phenylalanine 171 is a molecular brake for translesion synthesis across benzo[a]pyrene-guanine adducts by human DNA polymerase kappaAkira Sassa
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
Mutat Res 718:10-7. 2011..These results suggest that F171 functions as a molecular brake for TLS across BPDE-N(2)-dG by Pol κ and that the F171A derivative of Pol κ bypasses these DNA lesions more actively than does the wild-type enzyme...
Critical amino acids in human DNA polymerases eta and kappa involved in erroneous incorporation of oxidized nucleotidesAtsushi Katafuchi
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya Ku, Tokyo 158 8501, Japan
Nucleic Acids Res 38:859-67. 2010..These results suggested that amino acids at distinct positions in the active sites of Poleta and Polkappa might enhance 8-oxo-dGTP to favor the syn conformation, and thus direct its misincorporation into DNA...
Efficient and erroneous incorporation of oxidized DNA precursors by human DNA polymerase etaMasatomi Shimizu
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan
Biochemistry 46:5515-22. 2007..We propose that human DNA polymerase eta may participate in oxidative mutagenesis through the efficient and erroneous incorporation of oxidized dNTPs during DNA synthesis...
The steric gate amino acid tyrosine 112 is required for efficient mismatched-primer extension by human DNA polymerase kappaNaoko Niimi
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya Ku, Tokyo 158 8501, Japan
Biochemistry 48:4239-46. 2009..We conclude that the steric gate of hPolkappa is a major fidelity factor that regulates extension reactions from mismatched primer termini...
Miscoding properties of 2'-deoxyinosine, a nitric oxide-derived DNA Adduct, during translesion synthesis catalyzed by human DNA polymerasesManabu Yasui
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya, Tokyo 158 8501, Japan
J Mol Biol 377:1015-23. 2008..Thus, the dI adduct is a highly miscoding lesion capable of generating A-->G transition. This ()NO-induced adduct may play an important role in initiating inflammation-driven carcinogenesis...
Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strainsMasami Yamada
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo 158 8501, Japan
Mol Microbiol 86:1364-75. 2012..coli mutT compared with the mammalian counterparts lacking the 8-oxo-dGTP hydrolysing activities...
Involvement of Y-family DNA polymerases in mutagenesis caused by oxidized nucleotides in Escherichia coliMasami Yamada
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1, Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
J Bacteriol 188:4992-5. 2006..Mutator phenotypes in sod/fur strains were substantially diminished by deletion of dinB and/or umuDC. DNA polymerases IV and V may be involved in mutagenesis caused by incorporation of the oxidized deoxynucleoside triphosphates...
Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerasesMasatomi Shimizu
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
EMBO Rep 4:269-73. 2003..We also report that human DNA polymerase eta, a human Y-family DNA polymerase, incorporates the oxidized dNTPs in a similar erroneous manner...
