Research Topics
Genomes and Genes | T FukagawaSummaryAffiliation: National Institute of Genetics Country: Japan Publications
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Publications
Formation of a centromere-specific chromatin structureTatsuo Fukagawa
Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies Sokendai, Mishima, Japan
Epigenetics 7:672-5. 2012..The intriguing histone-like properties of these proteins suggest that they may form nucleosome-like structures at various genome loci, extending the chromatin code beyond classical histone variants...
Dicer is essential for formation of the heterochromatin structure in vertebrate cellsTatsuo Fukagawa
Precursory Research for Embryonic Science and Technology of Japan Science and Technology Agency, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Nat Cell Biol 6:784-91. 2004..We conclude that Dicer-related RNA interference machinery is involved in the formation of the heterochromatin structure in higher vertebrate cells...- Centromere DNA, proteins and kinetochore assembly in vertebrate cellsTatsuo Fukagawa
PRESTO, the Japan Science and Technology Agency JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Chromosome Res 12:557-67. 2004..Herein, I review recent advances in our understanding of centromeric DNAs as sites for kinetochore assembly and the mechanisms underlying kinetochore assembly in vertebrate cells... - The kinetochore and spindle checkpoint in vertebrate cellsTatsuo Fukagawa
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Front Biosci 13:2705-13. 2008..Herein, recent advances in our understanding of the kinetochore and spindle checkpoint in vertebrate cells are reviewed. I also review our recent contributions to this field and discuss their implications using chicken DT40 system... 
Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cellsT Fukagawa
National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Nucleic Acids Res 29:3796-803. 2001..The novel strategy reported here will be useful and applicable to a wide range of proteins that have general cell-autonomous function in vertebrate cells...- CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cellsT Fukagawa
National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
EMBO J 20:4603-17. 2001..These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H... - CENP-C is involved in chromosome segregation, mitotic checkpoint function, and kinetochore assemblyMi Sun Kwon
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Shizuoka 411 8540, Japan
Mol Biol Cell 18:2155-68. 2007..These results suggest that centromere localization of CENP-C in interphase nuclei occurs upstream of localization of the Mis12 complex and downstream of localization of the CENP-H complex... - CENP-I is essential for centromere function in vertebrate cellsAi Nishihashi
PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411 8540, Shizuoka, Japan
Dev Cell 2:463-76. 2002..Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere... - Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cellsTetsuya Hori
PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
J Cell Sci 116:3347-62. 2003..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic... - Gene structure, chromosomal localization and immunolocalization of chicken centromere proteins CENP-C and ZW10A Okamura
Department of Evolutionary Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411 8540, Shizuoka ken, Japan
Gene 262:283-90. 2001..During anaphase, chicken ZW10 proteins were no longer localized near chromosomes or the mitotic apparatus but were present diffusely in the cytoplasm... - The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeresMasahiro Okada
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Nat Cell Biol 8:446-57. 2006.... - CENP-O class proteins form a stable complex and are required for proper kinetochore functionTetsuya Hori
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima 411 8540, Japan
Mol Biol Cell 19:843-54. 2008..This suggests that CENP-O class proteins are involved in the prevention of premature sister chromatid separation during recovery from spindle damage... - The constitutive centromere component CENP-50 is required for recovery from spindle damageYukinori Minoshima
National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
Mol Cell Biol 25:10315-28. 2005..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage... - CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochoreTetsuya Hori
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
Cell 135:1039-52. 2008..In total, our results suggest that the CENP-T/CENP-W complex is directly involved in establishment of centromere chromatin structure coordinately with CENP-A... - Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complexT Tenzen
Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Shizuoka ken, Japan
Mol Cell Biol 17:4043-50. 1997..Because the nascent DNA in the switch region was recovered at low efficiency, we investigated whether this region is associated with the nuclear scaffold and found three scaffold-associated regions in and around the switch region... - The CENP-S complex is essential for the stable assembly of outer kinetochore structureMiho Amano
Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
J Cell Biol 186:173-82. 2009..In addition, we found that intrakinetochore distance was increased in CENP-S- and CENP-X-deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore... - The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosisYoshikazu Mikami
PRESTO of JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Mol Cell Biol 25:1958-70. 2005..On the basis of these results and previously published data, we propose that the Nuf2 complex functions as a connector between the inner and outer kinetochores... - Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3K Sugaya
Department of Evolutionary Genetics, National Institute of Genetics, Shizuoka ken, Japan
Genomics 23:408-19. 1994..It thus corresponds to the intact form of a Notch-type transmembrane protein. About 20 kb of dense Alu clustering was found just centromeric to the NOTCH3... - [Dynamics of the kinetochore proteins which target to the chromatin of centromeres]Tetsuya Hori
Tanpakushitsu Kakusan Koso 51:326-34. 2006 - Assembly of kinetochores in vertebrate cellsTatsuo Fukagawa
PRESTO, the Japan Science and Technology Agency JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
Exp Cell Res 296:21-7. 2004..In this review, I will focus on recent advances in our understanding of centromere DNAs as sites for kinetochore assembly and the mechanism underlying kinetochore assembly in vertebrate cells... - Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequencesWei Hao Shang
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
Genome Res 20:1219-28. 2010.... - The DT40 system as a tool for analyzing kinetochore assemblyMasahiro Okada
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan
Subcell Biochem 40:91-106. 2006..The DT40 system is a powerful and reliable tool for study of kinetochore assembly. Herein, we review recent advances in our understanding of the mechanisms underlying kinetochore assembly in DT40 cells... - Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and antitriplex antibodiesMizuki Ohno
Division of Evolutionary Genetics, Department of Population Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken, Japan
Chromosoma 111:201-13. 2002..Triplexes visualized differentially with distinct PuPy tract probes were associated spatially with centromeric sequences in the interphase nucleus in a sequence-specific manner... - Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite arrayJennifer M Spence
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
EMBO J 21:5269-80. 2002.... - Ubc9 is essential for viability of higher eukaryotic cellsTomoko Hayashi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, 980 8578, Japan
Exp Cell Res 280:212-21. 2002.... - KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebratesIain M Cheeseman
Ludwig Institute for Cancer Research, and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
Mol Biol Cell 19:587-94. 2008..These findings explain discrepancies in kinetochore assembly pathways between different organisms and reveal a surprising plasticity in the assembly mechanism of an essential cell division organelle... - Characterization of chicken CENP-A and comparative sequence analysis of vertebrate centromere-specific histone H3-like proteinsVinciane Regnier
Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, UK
Gene 316:39-46. 2003.... - Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cellsFumiyuki Sanematsu
Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889 1692, Japan
J Biol Chem 281:13817-27. 2006.... - The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functionsMichela Zuccolo
Centre de Recherche, UMR 144 CNRS, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
EMBO J 26:1853-64. 2007..Together, our data thus provide the first molecular clues underlying the function of the human Nup107-160 complex at kinetochores... - Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cellsYasunari Takami
Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, University of Miyazaki, Miyazaki 889 1692, Japan
Mol Biol Cell 18:129-41. 2007..These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA... - CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1Vinciane Regnier
Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, United Kingdom
Mol Cell Biol 25:3967-81. 2005..Our data thus point to a specific role for CENP-A in assembly of kinetochores competent in the maintenance of mitotic checkpoint signaling... 
The human Mis12 complex is required for kinetochore assembly and proper chromosome segregationSusan L Kline
Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA
J Cell Biol 173:9-17. 2006..These results indicate that a four-subunit Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly...- A novel histone exchange factor, protein phosphatase 2Cgamma, mediates the exchange and dephosphorylation of H2A-H2BHiroshi Kimura
Nuclear Function and Dynamics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Cell Biol 175:389-400. 2006.... - Cell-cycle-dependent pharmacology of methotrexate in HL-60Atsushi Yamauchi
Pharmaceutics, Division of Clinical Pharmacy, Department of Medico-Pharmaceutical Science, Faculty of Pharmaceutical Sciences, Kyushu University, Japan
J Pharmacol Sci 99:335-41. 2005..Therefore, the choice of dosing time associated with cell rhythmicity may help to achieve rational chronotherapeutics, increasing therapeutic effects...