T Fukagawa

Summary

Affiliation: National Institute of Genetics
Country: Japan

Publications

  1. pmc Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins
    Aussie Suzuki
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 193:125-40. 2011
  2. pmc The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly
    Tetsuya Hori
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 200:45-60. 2013
  3. pmc Chromosome engineering allows the efficient isolation of vertebrate neocentromeres
    Wei Hao Shang
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Dev Cell 24:635-48. 2013
  4. pmc The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA
    Kozo Takeuchi
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan, The Graduate University for Advanced Studies Sokendai, Mishima, Shizuoka 411 8540, Japan, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8581, Japan, JST, PRESTO, Fukuoka 812 8582, Japan and Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku ku, Tokyo 162 8480, Japan
    Nucleic Acids Res 42:1644-55. 2014
  5. doi request reprint Speciation mediated by centromeres
    Tatsuo Fukagawa
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan Electronic address
    Dev Cell 27:367-8. 2013
  6. doi request reprint Formation of a centromere-specific chromatin structure
    Tatsuo Fukagawa
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies Sokendai, Mishima, Japan
    Epigenetics 7:672-5. 2012
  7. ncbi request reprint Centromere DNA, proteins and kinetochore assembly in vertebrate cells
    Tatsuo Fukagawa
    PRESTO, the Japan Science and Technology Agency JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Chromosome Res 12:557-67. 2004
  8. pmc Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells
    T Fukagawa
    National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nucleic Acids Res 29:3796-803. 2001
  9. pmc CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells
    T Fukagawa
    National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    EMBO J 20:4603-17. 2001
  10. ncbi request reprint The kinetochore and spindle checkpoint in vertebrate cells
    Tatsuo Fukagawa
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Front Biosci 13:2705-13. 2008

Collaborators

Detail Information

Publications39

  1. pmc Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins
    Aussie Suzuki
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 193:125-40. 2011
    ....
  2. pmc The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly
    Tetsuya Hori
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 200:45-60. 2013
    ..Based on the analysis of these different engineered kinetochores, we conclude that the CCAN has two distinct roles: recruiting CENP-A to establish the kinetochore and serving as a structural core to directly recruit kinetochore proteins...
  3. pmc Chromosome engineering allows the efficient isolation of vertebrate neocentromeres
    Wei Hao Shang
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Dev Cell 24:635-48. 2013
    ..In summary, our experimental system provides valuable insights for understanding how neocentromeres form...
  4. pmc The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA
    Kozo Takeuchi
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan, The Graduate University for Advanced Studies Sokendai, Mishima, Shizuoka 411 8540, Japan, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8581, Japan, JST, PRESTO, Fukuoka 812 8582, Japan and Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku ku, Tokyo 162 8480, Japan
    Nucleic Acids Res 42:1644-55. 2014
    ..In summary, our work reveals the structural features and properties of the CENP-T-W-S-X complex for its localization to centromeres...
  5. doi request reprint Speciation mediated by centromeres
    Tatsuo Fukagawa
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan Electronic address
    Dev Cell 27:367-8. 2013
    ..In this issue of Developmental Cell, Thomae et al. (2013) demonstrate that Drosophila speciation factors HMR and LHR form a centromeric complex and that increased dosage causes hybrid lethality. ..
  6. doi request reprint Formation of a centromere-specific chromatin structure
    Tatsuo Fukagawa
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies Sokendai, Mishima, Japan
    Epigenetics 7:672-5. 2012
    ..The intriguing histone-like properties of these proteins suggest that they may form nucleosome-like structures at various genome loci, extending the chromatin code beyond classical histone variants...
  7. ncbi request reprint Centromere DNA, proteins and kinetochore assembly in vertebrate cells
    Tatsuo Fukagawa
    PRESTO, the Japan Science and Technology Agency JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Chromosome Res 12:557-67. 2004
    ..Herein, I review recent advances in our understanding of centromeric DNAs as sites for kinetochore assembly and the mechanisms underlying kinetochore assembly in vertebrate cells...
  8. pmc Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells
    T Fukagawa
    National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nucleic Acids Res 29:3796-803. 2001
    ..The novel strategy reported here will be useful and applicable to a wide range of proteins that have general cell-autonomous function in vertebrate cells...
  9. pmc CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells
    T Fukagawa
    National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    EMBO J 20:4603-17. 2001
    ..These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H...
  10. ncbi request reprint The kinetochore and spindle checkpoint in vertebrate cells
    Tatsuo Fukagawa
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Front Biosci 13:2705-13. 2008
    ..Herein, recent advances in our understanding of the kinetochore and spindle checkpoint in vertebrate cells are reviewed. I also review our recent contributions to this field and discuss their implications using chicken DT40 system...
  11. ncbi request reprint Dicer is essential for formation of the heterochromatin structure in vertebrate cells
    Tatsuo Fukagawa
    Precursory Research for Embryonic Science and Technology of Japan Science and Technology Agency, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 6:784-91. 2004
    ..We conclude that Dicer-related RNA interference machinery is involved in the formation of the heterochromatin structure in higher vertebrate cells...
  12. pmc CENP-C is involved in chromosome segregation, mitotic checkpoint function, and kinetochore assembly
    Mi Sun Kwon
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Shizuoka 411 8540, Japan
    Mol Biol Cell 18:2155-68. 2007
    ..These results suggest that centromere localization of CENP-C in interphase nuclei occurs upstream of localization of the Mis12 complex and downstream of localization of the CENP-H complex...
  13. ncbi request reprint CENP-I is essential for centromere function in vertebrate cells
    Ai Nishihashi
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411 8540, Shizuoka, Japan
    Dev Cell 2:463-76. 2002
    ..Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere...
  14. ncbi request reprint Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells
    Tetsuya Hori
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Sci 116:3347-62. 2003
    ..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic...
  15. ncbi request reprint Gene structure, chromosomal localization and immunolocalization of chicken centromere proteins CENP-C and ZW10
    A Okamura
    Department of Evolutionary Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411 8540, Shizuoka ken, Japan
    Gene 262:283-90. 2001
    ..During anaphase, chicken ZW10 proteins were no longer localized near chromosomes or the mitotic apparatus but were present diffusely in the cytoplasm...
  16. pmc The constitutive centromere component CENP-50 is required for recovery from spindle damage
    Yukinori Minoshima
    National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:10315-28. 2005
    ..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage...
  17. pmc CENP-O class proteins form a stable complex and are required for proper kinetochore function
    Tetsuya Hori
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima 411 8540, Japan
    Mol Biol Cell 19:843-54. 2008
    ..This suggests that CENP-O class proteins are involved in the prevention of premature sister chromatid separation during recovery from spindle damage...
  18. ncbi request reprint The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres
    Masahiro Okada
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 8:446-57. 2006
    ....
  19. ncbi request reprint CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore
    Tetsuya Hori
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Cell 135:1039-52. 2008
    ..In total, our results suggest that the CENP-T/CENP-W complex is directly involved in establishment of centromere chromatin structure coordinately with CENP-A...
  20. pmc Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex
    T Tenzen
    Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Shizuoka ken, Japan
    Mol Cell Biol 17:4043-50. 1997
    ..Because the nascent DNA in the switch region was recovered at low efficiency, we investigated whether this region is associated with the nuclear scaffold and found three scaffold-associated regions in and around the switch region...
  21. pmc The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis
    Yoshikazu Mikami
    PRESTO of JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:1958-70. 2005
    ..On the basis of these results and previously published data, we propose that the Nuf2 complex functions as a connector between the inner and outer kinetochores...
  22. pmc The CENP-S complex is essential for the stable assembly of outer kinetochore structure
    Miho Amano
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 186:173-82. 2009
    ..In addition, we found that intrakinetochore distance was increased in CENP-S- and CENP-X-deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore...
  23. ncbi request reprint Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3
    K Sugaya
    Department of Evolutionary Genetics, National Institute of Genetics, Shizuoka ken, Japan
    Genomics 23:408-19. 1994
    ..It thus corresponds to the intact form of a Notch-type transmembrane protein. About 20 kb of dense Alu clustering was found just centromeric to the NOTCH3...
  24. ncbi request reprint Assembly of kinetochores in vertebrate cells
    Tatsuo Fukagawa
    PRESTO, the Japan Science and Technology Agency JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Exp Cell Res 296:21-7. 2004
    ..In this review, I will focus on recent advances in our understanding of centromere DNAs as sites for kinetochore assembly and the mechanism underlying kinetochore assembly in vertebrate cells...
  25. ncbi request reprint [Dynamics of the kinetochore proteins which target to the chromatin of centromeres]
    Tetsuya Hori
    Tanpakushitsu Kakusan Koso 51:326-34. 2006
  26. pmc Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences
    Wei Hao Shang
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Genome Res 20:1219-28. 2010
    ....
  27. ncbi request reprint The DT40 system as a tool for analyzing kinetochore assembly
    Masahiro Okada
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Subcell Biochem 40:91-106. 2006
    ..The DT40 system is a powerful and reliable tool for study of kinetochore assembly. Herein, we review recent advances in our understanding of the mechanisms underlying kinetochore assembly in DT40 cells...
  28. ncbi request reprint Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and antitriplex antibodies
    Mizuki Ohno
    Division of Evolutionary Genetics, Department of Population Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka ken, Japan
    Chromosoma 111:201-13. 2002
    ..Triplexes visualized differentially with distinct PuPy tract probes were associated spatially with centromeric sequences in the interphase nucleus in a sequence-specific manner...
  29. pmc Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite array
    Jennifer M Spence
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    EMBO J 21:5269-80. 2002
    ....
  30. ncbi request reprint Ubc9 is essential for viability of higher eukaryotic cells
    Tomoko Hayashi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, 980 8578, Japan
    Exp Cell Res 280:212-21. 2002
    ....
  31. ncbi request reprint Characterization of chicken CENP-A and comparative sequence analysis of vertebrate centromere-specific histone H3-like proteins
    Vinciane Regnier
    Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, UK
    Gene 316:39-46. 2003
    ....
  32. pmc Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells
    Yasunari Takami
    Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, University of Miyazaki, Miyazaki 889 1692, Japan
    Mol Biol Cell 18:129-41. 2007
    ..These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA...
  33. pmc CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1
    Vinciane Regnier
    Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, United Kingdom
    Mol Cell Biol 25:3967-81. 2005
    ..Our data thus point to a specific role for CENP-A in assembly of kinetochores competent in the maintenance of mitotic checkpoint signaling...
  34. pmc The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation
    Susan L Kline
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA
    J Cell Biol 173:9-17. 2006
    ..These results indicate that a four-subunit Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly...
  35. ncbi request reprint Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells
    Fumiyuki Sanematsu
    Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889 1692, Japan
    J Biol Chem 281:13817-27. 2006
    ....
  36. pmc KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 19:587-94. 2008
    ..These findings explain discrepancies in kinetochore assembly pathways between different organisms and reveal a surprising plasticity in the assembly mechanism of an essential cell division organelle...
  37. pmc The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions
    Michela Zuccolo
    Centre de Recherche, UMR 144 CNRS, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    EMBO J 26:1853-64. 2007
    ..Together, our data thus provide the first molecular clues underlying the function of the human Nup107-160 complex at kinetochores...
  38. pmc A novel histone exchange factor, protein phosphatase 2Cgamma, mediates the exchange and dephosphorylation of H2A-H2B
    Hiroshi Kimura
    Nuclear Function and Dynamics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 175:389-400. 2006
    ....
  39. ncbi request reprint Cell-cycle-dependent pharmacology of methotrexate in HL-60
    Atsushi Yamauchi
    Pharmaceutics, Division of Clinical Pharmacy, Department of Medico Pharmaceutical Science, Faculty of Pharmaceutical Sciences, Kyushu University, Japan
    J Pharmacol Sci 99:335-41. 2005
    ..Therefore, the choice of dosing time associated with cell rhythmicity may help to achieve rational chronotherapeutics, increasing therapeutic effects...