Yasunori Chiba

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. ncbi request reprint Glycan engineering and production of 'humanized' glycoprotein in yeast cells
    Yasunori Chiba
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biol Pharm Bull 32:786-95. 2009
  2. doi request reprint Focused differential glycan analysis with the platform antibody-assisted lectin profiling for glycan-related biomarker verification
    Atsushi Kuno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2, 1 1 1, Umezono, Ibaraki 305 8568, Japan
    Mol Cell Proteomics 8:99-108. 2009
  3. pmc Engineering of mucin-type human glycoproteins in yeast cells
    Koh Amano
    Research Center for Medical Glycoscience and Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba 305 8566, Japan
    Proc Natl Acad Sci U S A 105:3232-7. 2008
  4. ncbi request reprint Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor
    Mika Kato Kaneko
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, C 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    FEBS Lett 581:331-6. 2007
  5. ncbi request reprint Functional expression of L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase from Bacteroides fragilis in Saccharomyces cerevisiae for the production of nucleotide sugars from exogenous monosaccharides
    Ta Wei Liu
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Glycobiology 21:1228-36. 2011
  6. ncbi request reprint Saccharomyces cerevisiae alpha1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule
    Toshihiko Kitajima
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, AIST Tsukuba Central 6, Tsukuba shi, Ibaraki, Japan
    FEBS J 273:5074-85. 2006
  7. ncbi request reprint Production of human beta-hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene
    Hiromi Akeboshi
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba 305 8566, Japan
    Glycobiology 19:1002-9. 2009
  8. doi request reprint In vitro and in vivo enzymatic syntheses and mass spectrometric database for N-glycans and o-glycans
    Hiromi Ito
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Open Space Laboratory C 2, Umezono, Tsukuba, Ibaraki, Japan
    Methods Enzymol 478:127-49. 2010
  9. doi request reprint Identification and characterization of endo-β-N-acetylglucosaminidase from methylotrophic yeast Ogataea minuta
    Satoshi Murakami
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1 1 1 Higashi, Tsukuba 305 8566, Japan
    Glycobiology 23:736-44. 2013
  10. ncbi request reprint Production of humanized glycoproteins in bacteria and yeasts
    Yasunori Chiba
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, Tsukuba, Japan
    Curr Opin Chem Biol 11:670-6. 2007

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Glycan engineering and production of 'humanized' glycoprotein in yeast cells
    Yasunori Chiba
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biol Pharm Bull 32:786-95. 2009
    ..Yeast glycoprotein expression systems are expected to become the dominant approach for the production of human glycoproteins in the near future...
  2. doi request reprint Focused differential glycan analysis with the platform antibody-assisted lectin profiling for glycan-related biomarker verification
    Atsushi Kuno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2, 1 1 1, Umezono, Ibaraki 305 8568, Japan
    Mol Cell Proteomics 8:99-108. 2009
    ..Thus, the established system should provide a powerful pipeline in support of on-going efforts in glyco-biomarker discovery...
  3. pmc Engineering of mucin-type human glycoproteins in yeast cells
    Koh Amano
    Research Center for Medical Glycoscience and Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba 305 8566, Japan
    Proc Natl Acad Sci U S A 105:3232-7. 2008
    ..The yeast system we describe here is able to produce glycoproteins modified at different glycosylation sites and has the potential for use in basic research and pharmaceutical applications...
  4. ncbi request reprint Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor
    Mika Kato Kaneko
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, C 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    FEBS Lett 581:331-6. 2007
    ..Sialic acid-deficient podoplanin recovered its activity after additional sialylation. These results indicated that the sialylated Corel at Thr52 is critical for podoplanin-induced platelet aggregation...
  5. ncbi request reprint Functional expression of L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase from Bacteroides fragilis in Saccharomyces cerevisiae for the production of nucleotide sugars from exogenous monosaccharides
    Ta Wei Liu
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2 OSL, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Glycobiology 21:1228-36. 2011
    ....
  6. ncbi request reprint Saccharomyces cerevisiae alpha1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule
    Toshihiko Kitajima
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, AIST Tsukuba Central 6, Tsukuba shi, Ibaraki, Japan
    FEBS J 273:5074-85. 2006
    ..Our results indicate that Och1p has the potential to transfer two mannoses from GDP-mannose, and strictly recognizes the overall structure of high mannose type oligosaccharide...
  7. ncbi request reprint Production of human beta-hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene
    Hiromi Akeboshi
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba 305 8566, Japan
    Glycobiology 19:1002-9. 2009
    ..These results suggest that beta-hexosaminidase A produced in a strain that overexpresses OmMNN4 will act as an effective enzyme for use in replacement therapy of GM2-gangliosidosis...
  8. doi request reprint In vitro and in vivo enzymatic syntheses and mass spectrometric database for N-glycans and o-glycans
    Hiromi Ito
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Open Space Laboratory C 2, Umezono, Tsukuba, Ibaraki, Japan
    Methods Enzymol 478:127-49. 2010
    ..Using the MS(n) spectral database, it is possible to identify glycan structures very easily and rapidly by spectral matching...
  9. doi request reprint Identification and characterization of endo-β-N-acetylglucosaminidase from methylotrophic yeast Ogataea minuta
    Satoshi Murakami
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1 1 1 Higashi, Tsukuba 305 8566, Japan
    Glycobiology 23:736-44. 2013
    ..Endo-Om also was able to hydrolyze N-glycans attached to RNase B and human transferrin under both denaturing and nondenaturing conditions. Thus, the present study reports the detection and characterization of a novel yeast ENGase...
  10. ncbi request reprint Production of humanized glycoproteins in bacteria and yeasts
    Yasunori Chiba
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, Tsukuba, Japan
    Curr Opin Chem Biol 11:670-6. 2007
    ....
  11. pmc Mutation of high-affinity methionine permease contributes to selenomethionyl protein production in Saccharomyces cerevisiae
    Toshihiko Kitajima
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Appl Environ Microbiol 76:6351-9. 2010
    ..Based on these results, we conclude that the mup1 mutant would be useful for the preparation of selenomethionyl proteins for X-ray crystallography...
  12. doi request reprint Use of novel selenomethionine-resistant yeast to produce selenomethionyl protein suitable for structural analysis
    Toshihiko Kitajima
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    FEMS Yeast Res 9:439-45. 2009
    ..Human lysozyme expressed by the mutant under the control of constitutive promoter contained selenomethionine at 65% occupancy, sufficient for use as a selenomethionine derivative for single-wavelength anomalous dispersion phasing...
  13. doi request reprint Glycoproteomic discovery of serological biomarker candidates for HCV/HBV infection-associated liver fibrosis and hepatocellular carcinoma
    Hiroyuki Kaji
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Proteome Res 12:2630-40. 2013
    ..These results indicate that our glycoproteomic strategy is effective for identifying multiple glyco-biomarker candidates in a high-throughput manner...
  14. ncbi request reprint [Remodeling of the sugar chain in yeast]
    Yoshifumi Jigami
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Seikagaku 78:5-15. 2006
  15. pmc Cytotoxic mechanism of selenomethionine in yeast
    Toshihiko Kitajima
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    J Biol Chem 287:10032-8. 2012
    ..Because cysteine is an intermediate of thiol compounds, these results suggested that the loss of a reduced form of thiol compounds due to selenomethionine causes a growth defect of yeast cells...
  16. pmc Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta
    Kousuke Kuroda
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 74:446-53. 2008
    ..In this study, we demonstrated the expression of human antibody in O. minuta and elucidated the relationship between O mannosylation and antibody production in yeast...
  17. ncbi request reprint Upregulation of genes involved in gluconeogenesis and the glyoxylate cycle suppressed the drug sensitivity of an N-glycan-deficient Saccharomyces cerevisiae mutant
    Hiroko Abe
    Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa, Japan
    Biosci Biotechnol Biochem 73:1398-403. 2009
    ..Furthermore, we found that YAB103 had a thicker cell-wall due to an increase in glucan content. To our knowledge, this is first report linking N-glycosylation to gluconeogenesis and the glyoxylate cycle...
  18. pmc Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice
    Shinji Takamatsu
    Department of Disease Glycomics, The Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, 567 0041, Japan
    Glycobiology 20:485-97. 2010
    ..This is the first report that mammalian organs have highly organized glycomic compensation systems to preserve N-glycan branch complexity...
  19. pmc Production of recombinant beta-hexosaminidase A, a potential enzyme for replacement therapy for Tay-Sachs and Sandhoff diseases, in the methylotrophic yeast Ogataea minuta
    Hiromi Akeboshi
    Research Center for Glycoscience, AIST Tsukuba Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki, Japan
    Appl Environ Microbiol 73:4805-12. 2007
    ..M6PHexA was incorporated dose dependently into GM2 gangliosidosis patient-derived fibroblasts via M6P receptors on the cell surface, and degradation of accumulated GM2 ganglioside was observed...
  20. ncbi request reprint Analysis of mannose-6-phosphate labeled with 8-aminopyrene-1,3,6-trisulfonate by capillary electrophoresis
    Minako Takashiba
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, 1 1 1, Higashi, Tsukuba, 305 8566, Japan
    Anal Biochem 332:196-8. 2004
  21. ncbi request reprint Inhibition of tumor cell-induced platelet aggregation using a novel anti-podoplanin antibody reacting with its platelet-aggregation-stimulating domain
    Yukinari Kato
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory C 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Biochem Biophys Res Commun 349:1301-7. 2006
    ..We infer that NZ-1 is useful to determine whether platelet aggregation is podoplanin-specific or not. Furthermore, podoplanin might become a therapeutic target of glioblastoma for antibody-based therapy...
  22. ncbi request reprint Production in yeast of alpha-galactosidase A, a lysosomal enzyme applicable to enzyme replacement therapy for Fabry disease
    Yasunori Chiba
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba 305 8566, Japan
    Glycobiology 12:821-8. 2002
    ..This method provides an effective and economic therapy for many lysosomal disorders, including Fabry disease...