Tomoko Akutsu

Summary

Affiliation: National Research Institute of Police Science
Country: Japan

Publications

  1. doi request reprint Specificity, sensitivity, and operability of RSID™-urine for forensic identification of urine: comparison with ELISA for Tamm-Horsfall protein
    Tomoko Akutsu
    Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, Kashiwa, Chiba, Japan
    J Forensic Sci 57:1570-3. 2012
  2. doi request reprint Detection of bacterial 16S ribosomal RNA genes for forensic identification of vaginal fluid
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Leg Med (Tokyo) 14:160-2. 2012
  3. doi request reprint Evaluation of latex agglutination tests for fibrin-fibrinogen degradation products in the forensic identification of menstrual blood
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Leg Med (Tokyo) 14:51-4. 2012
  4. ncbi request reprint Individual comparisons of the levels of (E)-3-methyl-2-hexenoic acid, an axillary odor-related compound, in Japanese
    Tomoko Akutsu
    Third Biology Section, Department of First Forensic Science, National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Chem Senses 31:557-63. 2006
  5. doi request reprint Evaluation of Tamm-Horsfall protein and uroplakin III for forensic identification of urine
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    J Forensic Sci 55:742-6. 2010
  6. ncbi request reprint Identification of human cytochrome p450 isozymes involved in diphenhydramine N-demethylation
    Tomoko Akutsu
    Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Chiba University, 1 8 1, Inohana, Chuo Ku, Chiba shi, Chiba 260 8675, Japan
    Drug Metab Dispos 35:72-8. 2007
  7. doi request reprint Applicability of ELISA detection of statherin for forensic identification of saliva
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba, 277 0882, Japan
    Int J Legal Med 124:493-8. 2010
  8. doi request reprint Detection of dermcidin for sweat identification by real-time RT-PCR and ELISA
    Koichi Sakurada
    National Research Institute of Police Science, Kashiwa, Chiba, Japan
    Forensic Sci Int 194:80-4. 2010
  9. ncbi request reprint Detection of herpes simplex virus type 1 DNA in bilateral human trigeminal ganglia and optic nerves by polymerase chain reaction
    Hisako Motani
    Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chuo Ku, Chiba, Japan
    J Med Virol 78:1584-7. 2006
  10. doi request reprint Expression of statherin mRNA and protein in nasal and vaginal secretions
    Koichi Sakurada
    National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba, Japan
    Leg Med (Tokyo) 13:309-13. 2011

Collaborators

Detail Information

Publications25

  1. doi request reprint Specificity, sensitivity, and operability of RSID™-urine for forensic identification of urine: comparison with ELISA for Tamm-Horsfall protein
    Tomoko Akutsu
    Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, Kashiwa, Chiba, Japan
    J Forensic Sci 57:1570-3. 2012
    ..Therefore, RSID™-Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP...
  2. doi request reprint Detection of bacterial 16S ribosomal RNA genes for forensic identification of vaginal fluid
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Leg Med (Tokyo) 14:160-2. 2012
    ..In conclusion, 16S ribosomal RNA genes of L. crispatus, L. jensenii and A. vaginae could be effective markers for forensic identification of vaginal fluid...
  3. doi request reprint Evaluation of latex agglutination tests for fibrin-fibrinogen degradation products in the forensic identification of menstrual blood
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Leg Med (Tokyo) 14:51-4. 2012
    ..FDP-P testing was shown to be a suitable and highly efficient rapid screening test for the laboratory identification of menstrual blood...
  4. ncbi request reprint Individual comparisons of the levels of (E)-3-methyl-2-hexenoic acid, an axillary odor-related compound, in Japanese
    Tomoko Akutsu
    Third Biology Section, Department of First Forensic Science, National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Chem Senses 31:557-63. 2006
    ..The results of this study indicate that E3M2H might contribute to axillary malodor in Asians as well as Caucasians...
  5. doi request reprint Evaluation of Tamm-Horsfall protein and uroplakin III for forensic identification of urine
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    J Forensic Sci 55:742-6. 2010
    ..However, ELISA and RT-PCR for UPIII were not specific for urine. UPIII may be applicable for forensic urine identification by immunohistochemistry...
  6. ncbi request reprint Identification of human cytochrome p450 isozymes involved in diphenhydramine N-demethylation
    Tomoko Akutsu
    Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Chiba University, 1 8 1, Inohana, Chuo Ku, Chiba shi, Chiba 260 8675, Japan
    Drug Metab Dispos 35:72-8. 2007
    ..In addition, large differences in the metabolic activities of CYP2D6 and those of CYP1A2, CYP2C9, and CYP2C19 could cause the individual differences in anti-allergic efficacy and the sedative effect of diphenhydramine...
  7. doi request reprint Applicability of ELISA detection of statherin for forensic identification of saliva
    Tomoko Akutsu
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba, 277 0882, Japan
    Int J Legal Med 124:493-8. 2010
    ..Forensic casework samples should be tested by ELISA detection or mRNA-based analysis for statherin, depending on the condition of the sample, to supplement presumptive tests for α-amylase, such as the Phadebas® amylase test...
  8. doi request reprint Detection of dermcidin for sweat identification by real-time RT-PCR and ELISA
    Koichi Sakurada
    National Research Institute of Police Science, Kashiwa, Chiba, Japan
    Forensic Sci Int 194:80-4. 2010
    ..In addition, the usefulness of ELISA was also verified. Positive sweat identification using these assays is expected to assist forensic practice...
  9. ncbi request reprint Detection of herpes simplex virus type 1 DNA in bilateral human trigeminal ganglia and optic nerves by polymerase chain reaction
    Hisako Motani
    Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chuo Ku, Chiba, Japan
    J Med Virol 78:1584-7. 2006
    ..Although the PCR-positive rate was significantly higher in advanced age, the correlation between the PCR-positive rate and gender was unclear. Additionally, HSV-1 DNA was not detected in any of the 50 optic nerve samples...
  10. doi request reprint Expression of statherin mRNA and protein in nasal and vaginal secretions
    Koichi Sakurada
    National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba, Japan
    Leg Med (Tokyo) 13:309-13. 2011
    ..However, the usefulness of these markers in practical forensic case samples has not yet been examined. Therefore, further research is required to establish the utility of these assays for identification of nasal secretions...
  11. doi request reprint Forensic validation of the modified ABO genotyping method using a DNA chip
    Ken Watanabe
    National Research Institute of Police Science, Chiba 277 0822, Japan
    Leg Med (Tokyo) 15:222-5. 2013
    ..5ng of DNA) is analyzed. Moreover, it was found that this chip method can be used to type highly degraded DNA. This method is expected to be a useful supplemental tool for the identification of individuals from highly decomposed samples...
  12. doi request reprint Identification of nasal blood by real-time RT-PCR
    Koichi Sakurada
    National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Leg Med (Tokyo) 14:201-4. 2012
    ..Future work should examine the usefulness of this assay under various environmental conditions...
  13. ncbi request reprint Forensic application of Epstein-Barr virus genotype: correlation between viral genotype and geographical area
    Hiroshi Ikegaya
    National Research Institute of Police Science, Chiba 277 0882, Japan
    J Virol Methods 147:78-85. 2008
    ..Typing in the EBV-associated nuclear antigen 2 gene was not related to that in the LMP-2A gene. Detection of the EBV genotype in the LMP-2A gene may be useful for determining the geographical origins of unidentified cadavers...
  14. doi request reprint A novel method for ABO genotyping using a DNA chip
    Ken Watanabe
    National Research Institute of Police Science, Chiba, Japan
    J Forensic Sci 56:S183-7. 2011
    ..This method fulfills the recent need for the development of rapid and convenient methods for criminal investigations...
  15. doi request reprint JC viral DNA chip allows geographical localization of unidentified cadavers for rapid identification
    Hiroshi Ikegaya
    National Research Institute of Police Science, 6 3 1 Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Forensic Sci Int Genet 2:54-60. 2008
    ..The analysis requires only a few hours to yield results. This new DNA chip method helps to narrow the search area for human identification much more quickly and easily than use of the conventional method...
  16. doi request reprint Evaluation of mRNA-based approach for identification of saliva and semen
    Koichi Sakurada
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Leg Med (Tokyo) 11:125-8. 2009
    ..5 years old), both of which had been kept under dry and dark conditions. The stability of mRNA beyond our supposition may play an important role in developing new techniques for body fluid identification...
  17. ncbi request reprint BK virus genotype distribution offers information of tracing the geographical origins of unidentified cadaver
    Hiroshi Ikegaya
    National Research Institute of Police Science, 277 0882 Chiba, Japan
    Forensic Sci Int 173:41-6. 2007
    ..Four subjects were positive for both the BKV and JCV. As the distribution areas of BKV and JCV genotypes are not identical, it is possible to narrow down the geographic area that any cadaver originates from...
  18. doi request reprint Mitochondrial DNA population data of HV1 and HV2 sequences from Japanese individuals
    Kazumasa Sekiguchi
    National Research Institute of Police Science, Fourth Biology Section, 6 3 1 Kashiwanoha, Kashiwa shi, Chiba 277 0882, Japan
    Leg Med (Tokyo) 10:284-6. 2008
    ..1C-315.1C) was found in 24 individuals. The haplotypes diversity and random match probability were calculated to be 0.9969 and 0.0040, respectively...
  19. doi request reprint Effects of oximes on mitochondrial oxidase activity
    Koichi Sakurada
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba 277 0882, Japan
    Toxicol Lett 189:110-4. 2009
    ..This hypothesis should be further investigated by in vivo study, including kinetic determination and free radical work...
  20. doi request reprint Evaluation of a co-extraction method for real-time PCR-based body fluid identification and DNA typing
    Ken Watanabe
    National Research Institute of Police Science, Chiba 277 0882, Japan Electronic address
    Leg Med (Tokyo) 16:56-9. 2014
    ..This method is promising for future forensic investigations because the use of this kit can reduce sample consumption for body fluid identification and DNA typing. ..
  21. ncbi request reprint Development of species identification tests targeting the 16S ribosomal RNA coding region in mitochondrial DNA
    Kazuhiko Imaizumi
    National Research Institute of Police Science, Kashiwa, Chiba, Japan
    Int J Legal Med 121:184-91. 2007
    ..From the results obtained, it was shown that this testing system could be a good tool for species identification. One successful case report is also demonstrated...
  22. ncbi request reprint Usefulness of dura mater in providing DNA samples for identifying cadavers
    Hisako Motani
    Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    J Forensic Sci 51:888-92. 2006
    ..The state of the DNA from the dura mater from the calvaria may be better than that from the basis cranii interna. We found that DNA from dura mater is one of the most useful samples for forensic identification...
  23. ncbi request reprint Hydrolysis of an acetylthiocholine by pralidoxime iodide (2-PAM)
    Koichi Sakurada
    National Research Institute of Police Science, 6 3 1, Kashiwanoha, Kashiwa, Chiba, Japan
    Toxicol Lett 166:255-60. 2006
    ..It is clear that oximes easily hydrolyze ASCh. We therefore strongly caution that the method of determining AChE activity with ASCh is not suitable for examining the effects of oximes...
  24. ncbi request reprint Blood-brain barrier penetration of novel pyridinealdoxime methiodide (PAM)-type oximes examined by brain microdialysis with LC-MS/MS
    Sou Okuno
    Section of Forensic Medicine, Department of International Health Development, Division of Public Health, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo, Japan
    Toxicol Appl Pharmacol 227:8-15. 2008
    ..However, the toxicity of 4-PAO (LD(50); 8.89 mg/kg) was greater than that of 2-PAM. Further investigation is required to determine the effects of these alkylPAMs in organophosphate poisoning...
  25. ncbi request reprint Rapid SMN1 deletion test using DHPLC to screen patients with spinal muscular atrophy
    Retno Sutomo
    Am J Med Genet 113:225-6. 2002