National Institute of Bioscience and Human-Technology

Summary

Organization: National Institute of Bioscience and Human-Technology
Country: Japan

Top Publications

  1. ncbi Evidence for polyphyletic origin of the members of the orders of Oscillatoriales and Pleurocapsales as determined by 16S rDNA analysis
    T Ishida
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    FEMS Microbiol Lett 201:79-82. 2001
  2. ncbi Effect of light/dark cycle on bacterial hydrogen production by Rhodobacter sphaeroides RV. From hour to second range
    T Wakayama
    National Institute of Bioscience and Human Technology, AIST MITI, Ibaraki, Japan
    Appl Biochem Biotechnol 84:431-40. 2000
  3. ncbi Effect of nystatin on the release of glycerol from salt-stressed cells of the salt-tolerant yeast Zygosaccharomyces rouxii
    K Hosono
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI, Tsukuba, Japan
    Arch Microbiol 173:284-7. 2000
  4. pmc Simultaneous direct counting of total and specific microbial cells in seawater, using a deep-sea microbe as target
    A Maruyama
    Bioconsortium Analysis Program, National Institute of Bioscience and Human Technology, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 66:2211-5. 2000
  5. ncbi Crystallization and preliminary X-ray study of Pk--REC from a hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1
    K Harata
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Acta Crystallogr D Biol Crystallogr 56:648-9. 2000
  6. ncbi Crystal structures of Urtica dioica agglutinin and its complex with tri-N-acetylchitotriose
    K Harata
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki, 305 8566, Japan
    J Mol Biol 297:673-81. 2000
  7. ncbi A tyramine receptor gene mutation causes a defective olfactory behavior in Drosophila melanogaster
    M Kutsukake
    Department of Biology, Ochanomizu University, Bunkyo ku, Tokyo, Japan
    Gene 245:31-42. 2000
  8. ncbi cis-acting regulatory sequences for antitermination in the transcript of the Bacillus subtilis hut operon and histidine-dependent binding of HutP to the transcript containing the regulatory sequences
    M Oda
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI, Tsukuba City, Ibaraki 305 8566, Japan
    Mol Microbiol 35:1244-54. 2000
  9. ncbi The prepro vasoactive intestinal contractor (VIC)/endothelin-2 gene (EDN2): structure, evolution, production, and embryonic expression
    K Saida
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, 305 8566, Japan
    Genomics 64:51-61. 2000
  10. ncbi Crystal structure of asparagine 233-replaced cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011 determined at 1.9 A resolution
    N Ishii
    Biophysical Chemistry Laboratory, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    J Mol Recognit 13:35-43. 2000

Scientific Experts

Detail Information

Publications62

  1. ncbi Evidence for polyphyletic origin of the members of the orders of Oscillatoriales and Pleurocapsales as determined by 16S rDNA analysis
    T Ishida
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    FEMS Microbiol Lett 201:79-82. 2001
    ..The results strongly indicate that even morphology was not necessarily reflected in the phylogenetic relationships at the order level. A need exists to reconstruct the taxonomy of cyanobacteria at the order level...
  2. ncbi Effect of light/dark cycle on bacterial hydrogen production by Rhodobacter sphaeroides RV. From hour to second range
    T Wakayama
    National Institute of Bioscience and Human Technology, AIST MITI, Ibaraki, Japan
    Appl Biochem Biotechnol 84:431-40. 2000
    ..Under excessive light intensity (1.2 kW.m-2), the light-to-hydrogen conversion efficiency was greatly enhanced. The hydrogen production rate during the 30-min cycle was twice as high as during a 12-h cycle under the same conditions...
  3. ncbi Effect of nystatin on the release of glycerol from salt-stressed cells of the salt-tolerant yeast Zygosaccharomyces rouxii
    K Hosono
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI, Tsukuba, Japan
    Arch Microbiol 173:284-7. 2000
    ..11 microM nystatin and 2% (w/v) glucose. Glycerol was indispensable for the growth of Z. rouxii grown in culture medium containing 15% NaCl...
  4. pmc Simultaneous direct counting of total and specific microbial cells in seawater, using a deep-sea microbe as target
    A Maruyama
    Bioconsortium Analysis Program, National Institute of Bioscience and Human Technology, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 66:2211-5. 2000
    ..Target cells immobilized on the filter were also successfully enumerated after stringent 3-cycle hybridization and even after a 16-month preservation at -30 degrees C...
  5. ncbi Crystallization and preliminary X-ray study of Pk--REC from a hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1
    K Harata
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Acta Crystallogr D Biol Crystallogr 56:648-9. 2000
    ..The unit-cell parameters were a = 151, b = 174, c = 241 A for form I and a = 151, b = 176, c = 300 A for form II, indicating that the asymmetric unit contains more than 20 molecules...
  6. ncbi Crystal structures of Urtica dioica agglutinin and its complex with tri-N-acetylchitotriose
    K Harata
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki, 305 8566, Japan
    J Mol Biol 297:673-81. 2000
    ..The third subsite of the N-terminal domain binds a NAG moiety with hydrogen bonds. The results suggest that the triad of aromatic amino acid residues is intrinsic in sugar binding of hevein-like domains...
  7. ncbi A tyramine receptor gene mutation causes a defective olfactory behavior in Drosophila melanogaster
    M Kutsukake
    Department of Biology, Ochanomizu University, Bunkyo ku, Tokyo, Japan
    Gene 245:31-42. 2000
    ..This study provides the first step toward understanding of the molecular genetics of tyramine-mediated neural functions in Drosophila...
  8. ncbi cis-acting regulatory sequences for antitermination in the transcript of the Bacillus subtilis hut operon and histidine-dependent binding of HutP to the transcript containing the regulatory sequences
    M Oda
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI, Tsukuba City, Ibaraki 305 8566, Japan
    Mol Microbiol 35:1244-54. 2000
    ....
  9. ncbi The prepro vasoactive intestinal contractor (VIC)/endothelin-2 gene (EDN2): structure, evolution, production, and embryonic expression
    K Saida
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, 305 8566, Japan
    Genomics 64:51-61. 2000
    ..This study provides the basis for disruption and regulation analysis of the gene, which may lead to a better understanding of VIC/ET2's physiological significance...
  10. ncbi Crystal structure of asparagine 233-replaced cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011 determined at 1.9 A resolution
    N Ishii
    Biophysical Chemistry Laboratory, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    J Mol Recognit 13:35-43. 2000
    ....
  11. ncbi Novel substrate specificity of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii
    I Matsui
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan
    FEBS Lett 467:195-200. 2000
    ....
  12. pmc Endosymbiotic microbiota of the bamboo pseudococcid Antonina crawii (Insecta, Homoptera)
    T Fukatsu
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, 305 8566, Japan
    Appl Environ Microbiol 66:643-50. 2000
    ..crawii...
  13. ncbi Calorimetric characterization of critical targets for killing and acquired thermotolerance in yeast
    K Obuchi
    National Institute of Bioscience and Human Technology, AIST, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Yeast 16:111-9. 2000
    ..This agreement suggests that the microsomal fraction may contain critical targets, and that trehalose accumulation in the heat-shocked and in the stationary phase yeast cells is a stabilizer of cellular components...
  14. ncbi Protein-carbohydrate interactions in human lysozyme probed by combining site-directed mutagenesis and affinity labeling
    M Muraki
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Biochemistry 39:292-9. 2000
    ..The structural analysis suggested that CH-pi interactions played an important role in the recognition of the carbohydrate residue at subsite B of HL...
  15. ncbi Brachycephalization in Japan has ceased
    M Kouchi
    Human Environment System Department, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    Am J Phys Anthropol 112:339-47. 2000
    ..Increases in lateral growth in Asian heads may be related to the facial flatness which is characteristic to northern Mongoloid populations...
  16. ncbi Chemically prepared hevein domains: effect of C-terminal truncation and the mutagenesis of aromatic residues on the affinity for chitin
    M Muraki
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Protein Eng 13:385-9. 2000
    ..The affinity of an HD for chitin might be improved by adjusting the size and substituent group of stacking aromatic rings...
  17. ncbi Increased sensitivity to the stimulant effects of morphine conferred by anti-adhesive glycoprotein SPARC in amygdala
    M Ikemoto
    Department of Biomolecular Engineering, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Nat Med 6:910-5. 2000
    ..Thus, SPARC may be an important element for establishing locomotor sensitization to morphine...
  18. ncbi Feature-based control of attention: evidence for two forms of dimension weighting
    T Kumada
    Human Informatics Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Percept Psychophys 63:698-708. 2001
    ..ITF was correlated with type of response, suggesting dimension-specific response mechanisms (Cohen & Shoup, 1997)...
  19. ncbi Desulfurization of benzothiophene by the Gram-negative bacterium, Sinorhizobium sp. KT55
    Y Tanaka
    Tsukuba Branch of Bio Refining Process Laboratory, Advanced Technology and Research Institute, Japan Petroleum Energy Center, 1 1 Higashi Tsukuba, Ibaraki 305 8566, Japan
    Curr Microbiol 43:187-91. 2001
    ..Desulfurization activity of this strain was significantly repressed by methionine, cysteine, sulfate, dimethyl sulfoxide, and Casamino acids...
  20. pmc Spiroplasma symbiont of the pea aphid, Acyrthosiphon pisum (Insecta: Homoptera)
    T Fukatsu
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba 305 8566, Japan
    Appl Environ Microbiol 67:1284-91. 2001
    ..Of 58 clonal strains of A. pisum established from natural populations in central Japan, 4 strains possessed the Spiroplasma organism...
  21. ncbi Eye fixation related potentials in a proof reading task
    Y Takeda
    Department of Psychology, Kwansei Gakuin University, Uegahara, Nishinomiya, 662 8501, Hyogo, Japan
    Int J Psychophysiol 40:181-6. 2001
    ..The error rate, the performance and the score of fatigue increased at the end of the task. EFRP would reflect decline of mental concentration (i.e. carelessness) caused by visual fatigue...
  22. ncbi Intracellular transport of phosphatidic acid and phosphatidylcholine into lipid bodies: use of fluorescent lipids to study lipid-body formation in an oleaginous fungus
    Y Kamisaka
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    Biochem Soc Trans 28:723-5. 2000
    ..The results dissect lipid transport of phosphatidic acid and phosphatidylcholine into lipid bodies and reveal regulatory steps for lipid-body formation in this fungus...
  23. pmc Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3
    K Ishikawa
    National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305, Japan
    Appl Environ Microbiol 67:673-9. 2001
    ..Some characteristics of this enzyme suggested that carboxypeptidase and aminoacylase might have evolved from a common origin...
  24. ncbi Crystal structures of heptakis(2,6-di-O-ethyl)cyclomaltoheptaose
    K Harata
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Carbohydr Res 329:597-607. 2000
    ..The result suggests the important role of solvent in the formation of helical arrangement of DE-beta-CD molecules...
  25. pmc Evidence for contribution of neutral trehalase in barotolerance of Saccharomyces cerevisiae
    H Iwahashi
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305, Japan
    Appl Environ Microbiol 66:5182-5. 2000
    ..Our results suggest that neutral trehalase contributes to barotolerance, especially during recovery...
  26. ncbi Interaction of nuclear proteins with intrinsically curved DNA in a matrix attachment region of a tobacco gene
    Y Fukuda
    Molecular Biology Department, National Institute of Bioscience and Human Technology, AIST, MITI, Tsukuba, Ibaraki, Japan
    Plant Mol Biol 44:91-8. 2000
    ..The possible functions of the binding proteins and their relationship to previously identified nuclear proteins, such as high-mobility-group proteins, are discussed...
  27. pmc Phylogenetic analysis of and oligonucleotide probe development for eikelboom type 021N filamentous bacteria isolated from bulking activated sludge
    T Kanagawa
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba 305 8566, Japan
    Appl Environ Microbiol 66:5043-52. 2000
    ....
  28. ncbi Analysis of 3-D human foot forms using the Free Form Deformation method and its application in grading shoe lasts
    M Mochimaru
    National Institute of Biosciences and Human Technology, Higashi, Tsukuba, Japan
    Ergonomics 43:1301-13. 2000
    ..It was found that the present method with FFD is not only useful for classifying 3-D human body forms, but also has potential as applications for designing well-fitting products...
  29. ncbi Inhibitory tagging in visual search can be found if search stimuli remain visible
    Y Takeda
    Kumada Group, Human Informatics Department, National Institute of Bioscience and Human Technology, Ibaraki, Japan
    Percept Psychophys 62:927-34. 2000
    ..In the present study, we found that evidence of inhibitory tagging could be observed only when the items of the search tasks were maintained until the responses for the small probes were made. This appeared to be an object-based effect...
  30. pmc The Rab3 GDP/GTP exchange factor homolog AEX-3 has a dual function in synaptic transmission
    K Iwasaki
    Laboratory of Molecular Neurobiology, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    EMBO J 19:4806-16. 2000
    ..We propose that connection of multiple pathways may be an important feature of Rab GEFs to coordinate various cellular events...
  31. ncbi Acetone preservation: a practical technique for molecular analysis
    T Fukatsu
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Mol Ecol 8:1935-45. 1999
    ....
  32. ncbi Second transmembrane segment of FtsH plays a role in its proteolytic activity and homo-oligomerization
    S Makino
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo
    FEBS Lett 460:554-8. 1999
    ..Therefore, the second transmembrane segment, which neighbors the C-terminal cytoplasmic region of the FtsH, participates in not only its membrane-anchoring, but also its protease activity and homo-oligomerization...
  33. ncbi High-throughput flow-injection analysis of glucose and glutamate in food and biological samples by using enzyme/polyion complex-bilayer membrane-based electrodes as the detectors
    F Mizutani
    National Institute of Bioscience and Human Technology, Ibaraki, Japan
    Biosens Bioelectron 13:809-15. 1998
    ..An FIA system for determining L-glutamic acid (3 microM-0.5 mM) was also prepared by using an enzyme electrode based on a glutamate oxidase/polyion complex-bilayer as the detector...
  34. ncbi Stabilization of two families of critical targets for hyperthermic cell killing and acquired thermotolerance of yeast cells
    K Obuchi
    National Institute of Bioscience and Human Technology, AIST, Ibaraki, Japan
    Yeast 14:1249-55. 1998
    ..On the other hand, both heat-shock response and arrest in stationary phase stabilizes the other family of targets that, in addition to the previous one, is responsible for phase 2 death...
  35. pmc Evidence for the interplay between trehalose metabolism and Hsp104 in yeast
    H Iwahashi
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 64:4614-7. 1998
    ..These results suggest that during heat shock, Hsp104 contributes to the simultaneous increase in both accumulation and degradation of trehalose...
  36. pmc Two intracellular symbiotic bacteria from the mulberry psyllid Anomoneura mori (Insecta, Homoptera)
    T Fukatsu
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 64:3599-606. 1998
    ..The nucleotide compositions of the 16S rDNAs of the X- and Y-symbionts were highly AT biased, and the sequence of the X-symbiont was the most AT-rich bacterial 16S rDNA sequence reported so far...
  37. ncbi Topological mutation of Escherichia coli dihydrofolate reductase
    M Iwakura
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, 305 8566, Japan
    J Biochem 124:769-77. 1998
    ..0 and 4.8 kcal/mol. Whereas, the Km and DeltaGH2O values for wild-type DHFR were 0.9 microM and 6.1 kcal/mol, respectively. The significance of the topological mutations was discussed with respect to protein folding and protein evolution...
  38. ncbi Survival and synaptogenesis of hippocampal neurons without NMDA receptor function in culture
    S Okabe
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Eur J Neurosci 10:2192-8. 1998
    ..In contrast, there is clear effect on neuronal survival. This finding establishes neuron number in standard culture conditions as a measure of NMDA receptor activity...
  39. ncbi Regional differences between the immunohistochemical distribution of Ca2+/calmodulin-dependent protein kinase II alpha and beta isoforms in the brainstem of the rat
    T Ochiishi
    Biosignalling Department, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, M I T I, Tsukuba, Ibaraki 305 8566, Japan
    Brain Res 790:129-40. 1998
    ..In conclusion, the present study confirmed that alpha- and beta-isoforms of CaM kinase II are differentially expressed in the nuclei of the brainstem and have different roles...
  40. ncbi Proceedings of the schistosome genome project
    M Tanaka
    National Institute of Bioscience and Human Technology, Institute of Industrial Science and Technology, Tsukuba Science City, Ibaraki, Japan
    Mem Inst Oswaldo Cruz 92:829-34. 1997
    ..e., successful growth, development, maturation and reproduction of the parasite within its host in the near future...
  41. ncbi Triplet repeat-containing ribosomal protein L14 gene in immortalized human endothelial cell line (t-HUE4)
    M Tanaka
    National Institute of Bioscience and Human Technology, Ibaraki, Japan
    Biochem Biophys Res Commun 243:531-7. 1998
    ..This GCT repeat was found to be one of the longest uninterrupted repeats in a coding sequence, which were associated with the highest degree of polymorphism...
  42. ncbi Effect of temperature on the role of Hsp104 and trehalose in barotolerance of Saccharomyces cerevisiae
    H Iwahashi
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan
    FEBS Lett 416:1-5. 1997
    ..These results suggest that the role of Hsp104 in barotolerance is temperature dependent in contrast to trehalose...
  43. pmc The role of Gcr1p in the transcriptional activation of glycolytic genes in yeast Saccharomyces cerevisiae
    H Uemura
    Department of Molecular Biology, National Institute of Bioscience and Human Technology, Tsukuba Research Center, Ibaraki, Japan
    Genetics 147:521-32. 1997
    ..These results support the hypothesis that Gcr1p and Gcr2p play the central role in glycolytic gene expression and that the function of Rap1p is to facilitate the binding of Gcr1p to its target...
  44. ncbi Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring
    S Hanada
    Department of Biology, Tokyo Metropolitan University, Hachioji, Japan
    Int J Syst Bacteriol 47:408-13. 1997
    ..neustonensis. Consequently, we assign the new isolate to the genus Porphyrobacter and propose the name Porphyrobacter tepidarius sp. nov. for it; the type strain of P. tepidarius is strain OT3 (= DSM 10595)...
  45. pmc Importance of van der Waals contact between Glu 35 and Trp 109 to the catalytic action of human lysozyme
    M Muraki
    Biomolecules Department, National Institute of Bioscience and Human Technology, Ibaraki, Japan
    Protein Sci 6:473-6. 1997
    ..The results revealed that the van der Waals contact between Glu 35 and Trp 109 was an essential determinant in the catalytic action of HL...
  46. pmc Detailed analysis of stem I and its 5' and 3' neighbor regions in the trans-acting HDV ribozyme
    F Nishikawa
    National Institute of Bioscience and Human Technology, AIST, MITI, 1 1 Higashi, Tsukuba Science City, Ibaraki 305 8566, Japan
    Nucleic Acids Res 27:403-10. 1999
    ..We also studied the substrate damaging sequences linked to both ends of stem I for its development in therapeutic applications and confirmed the functions of the unique structure...
  47. ncbi Mannosylphosphate transfer to yeast mannan
    Y Jigami
    National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Biochim Biophys Acta 1426:335-45. 1999
    ..Glycans in pathogenic yeast and protozoa are also modified with mannosylphosphate, and the potential contribution of this modification to the pathogenicity of these organisms is evaluated...
  48. pmc Phylogenetic diversity of symbiotic methanogens living in the hindgut of the lower termite Reticulitermes speratus analyzed by PCR and in situ hybridization
    N Shinzato
    Department of Biology, School of Arts and Science, University of Tokyo, Komaba, Meguro ku, Tokyo 153 0041, Japan
    Appl Environ Microbiol 65:837-40. 1999
    ..Three clones were classified in the order Thermoplasmales. The Methanobrevibacter-related symbionts were detected by in situ hybridization analysis...
  49. ncbi Folding energetics of a multidomain protein, flagellin
    S Honda
    National Institute of Bioscience and Human Technology, Higashi, Tsukuba, 305 8566, Japan
    J Mol Biol 293:719-32. 1999
    ..These characteristics suggest that flagellin is organized with relatively flexible domains and rigid domains, which appears to be responsible for the well-regulated assembly mechanism of the bacterial flagellar filament...
  50. ncbi Shape and energetics of a cavity in c-Myb probed by natural and non-natural amino acid mutations
    H Morii
    National Institute of Bioscience and Human technology NIBH, Tsukuba, Ibaraki, 305 8566, Japan
    J Mol Biol 292:909-20. 1999
    ..Therefore, the increase in unfolding free energy would most likely be attributed to van der Waals interactions in the cavity wall, which would be a origin of stabilization by the sliding of tryptophan 95 into the cavity upon DNA binding...
  51. pmc Biological clocks
    N Ishida
    Ishida Group of Clock Gene, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry, 1 1 Higashi, Tsukuba Science City, Ibaraki 305 Japan
    Proc Natl Acad Sci U S A 96:8819-20. 1999
    ..The intent of this summary is to introduce the common molecular mechanism of biological clocks between flies and humans and then to describe the research from three laboratories that was presented in the session...
  52. ncbi DNA bend sites in the human beta-globin locus: evidence for a basic and universal structural component of genomic DNA
    Y Wada-Kiyama
    Department of Physiology, Nippon Medical School, Tokyo, Japan
    Mol Biol Evol 16:922-30. 1999
    ..These lines of evidence suggest that these DNA bend sites are one of the basic structural components universally present in genomic DNA...
  53. ncbi Identification of a 55-kDa ezrin-related protein that induces cytoskeletal changes and localizes to the nucleolus
    S C Kaul
    National Institute of Bioscience and Human Technology, AIST, 1 1 Higashi, Ibaraki, Tsukuba Science City, 305 8566, Japan
    Exp Cell Res 250:51-61. 1999
    ..These data demonstrate the existence of an N-terminal cleavage form of ezrin that localizes to the nucleolus and that its overexpression induces cytoskeletal changes...
  54. ncbi Protective effect of acidic fibroblast growth factor against ischemia-induced learning and memory deficits in two tasks in gerbils
    A J Li
    Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Physiol Behav 66:577-83. 1999
    ..These observations suggest that the hippocampus is a critical site for the performance of the two tasks, and that aFGF has a protective effect against such ischemia-induced learning and memory deficits in gerbils...
  55. ncbi Magnetic fields produced by single motor units in human skeletal muscles
    T Masuda
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI, Tsukuba, Ibaraki, Japan
    Clin Neurophysiol 110:384-9. 1999
    ....
  56. ncbi Isolation and sequence of the GCR3 homologue from Candida albicans by complementation of (delta)gcr3 strain of Saccharomyces cerevisiae
    H Uemura
    Department of Molecular Biology, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan
    Yeast 15:323-7. 1999
    ..Transformants of this gene also recovered the glycolytic enzyme activities. Its DNA sequencing predicted an 886 amino acid protein with 30.4% identity to the Gcr3p of S. cerevisiae...
  57. ncbi Multineuronal spike classification based on multisite electrode recording, whole-waveform analysis, and hierarchical clustering
    H Kaneko
    National Institute of Bioscience and Human Technology, Ibaraki, Japan
    IEEE Trans Biomed Eng 46:280-90. 1999
    ..Waveforms of classified spikes were well superimposed. We concluded that this method is particularly useful for separating the activities of adjacent neurons that fire partially overlapping spikes and/or complex spike bursts...
  58. ncbi A group I intron in the 18S ribosomal DNA from the parasitic fungus Isaria japonica
    Y Ito
    Biomolecules Department, National Institute of Bioscience and Human Technology, Ibaraki, Japan
    J Mol Evol 48:337-40. 1999
    ..This is in contrast to the sequence identity found on the neighboring rDNA. This is an interesting finding and suggests a horizontal transfer of group I intron sequences...
  59. ncbi Crystallographic evaluation of internal motion of human alpha-lactalbumin refined by full-matrix least-squares method
    K Harata
    Biomolecules Department, National Institute of Bioscience and Human Technology, 1 1 Higashi, Tsukuba, Ibaraki, 305 8566, Japan
    J Mol Biol 287:347-58. 1999
    ..The result indicates that the internal motion is closely related to biological function of proteins...
  60. ncbi Fragment reconstitution of a small protein: folding energetics of the reconstituted immunoglobulin binding domain B1 of streptococcal protein G
    S Honda
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    Biochemistry 38:1203-13. 1999
    ....
  61. ncbi Temporal process from receptors to higher brain in taste detection studied by gustatory-evoked magnetic fields and reaction times
    S Saito
    National Institute of Bioscience and Human Technology, Ibaraki, Japan
    Ann N Y Acad Sci 855:493-7. 1998
    ..With increased concentrations of NaCl, RT became shorter, but onset latencies of GEM remained constant. Sweet taste took a longer time than salty taste at receptor process including the time for diffusion to receptors...
  62. pmc Isocitrate dehydrogenase from Thermus aquaticus YT1: purification of the enzyme and cloning, sequencing, and expression of the gene
    K Miyazaki
    National Institute of Bioscience and Human Technology, Ibaraki, Japan
    Appl Environ Microbiol 62:4627-31. 1996
    ..The recombinant enzyme showed high thermostability and almost the same heat denaturation profile as the intact enzyme purified from the thermophile cells, implying that the recombinant protein has the same structure as the intact one...