Akiho Yokota

Summary

Affiliation: Nara Institute of Science and Technology
Country: Japan

Publications

  1. ncbi request reprint Citrulline and DRIP-1 protein (ArgE homologue) in drought tolerance of wild watermelon
    Akiho Yokota
    Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan
    Ann Bot 89:825-32. 2002
  2. pmc Bacterial variations on the methionine salvage pathway
    Agnieszka Sekowska
    HKU Pasteur Research Centre, 8, Sassoon Road, Pokfulam, Hong Kong, SAR Hong Kong
    BMC Microbiol 4:9. 2004
  3. ncbi request reprint Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon
    Kentaro Takahara
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Japan
    FEBS J 272:5353-64. 2005
  4. pmc Structural and functional similarities between a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-like protein from Bacillus subtilis and photosynthetic RuBisCO
    Yohtaro Saito
    Nara Institute of Science and Technology, Graduate School of Biological Sciences, Ikoma, Nara, Japan
    J Biol Chem 284:13256-64. 2009
  5. doi request reprint Responses of the photosynthetic electron transport system to excess light energy caused by water deficit in wild watermelon
    Satoko Sanda
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    Physiol Plant 142:247-64. 2011
  6. ncbi request reprint Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon
    Kinya Akashi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara 630 0101, Japan
    Biochem Biophys Res Commun 323:72-8. 2004
  7. ncbi request reprint Co-expression of cytochrome b561 and ascorbate oxidase in leaves of wild watermelon under drought and high light conditions
    Yoshihiko Nanasato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630 0192 Japan
    Plant Cell Physiol 46:1515-24. 2005
  8. doi request reprint An evolutionally conserved Lys122 is essential for function in Rhodospirillum rubrum bona fide RuBisCO and Bacillus subtilis RuBisCO-like protein
    Toshihiro Nakano
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Biochem Biophys Res Commun 392:212-6. 2010
  9. pmc A phosphofructokinase B-type carbohydrate kinase family protein, NARA5, for massive expressions of plastid-encoded photosynthetic genes in Arabidopsis
    Taro Ogawa
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Plant Physiol 151:114-28. 2009
  10. doi request reprint Dynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit
    Kinya Akashi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara 630 0192, Japan
    Planta 233:947-60. 2011

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Citrulline and DRIP-1 protein (ArgE homologue) in drought tolerance of wild watermelon
    Akiho Yokota
    Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan
    Ann Bot 89:825-32. 2002
    ..The salt treatment causes the accumulation of gamma-aminobutyrate, glutamine and alanine, in addition to a smaller amount of citrulline. Citrulline may function as a potent hydroxyl radical scavenger...
  2. pmc Bacterial variations on the methionine salvage pathway
    Agnieszka Sekowska
    HKU Pasteur Research Centre, 8, Sassoon Road, Pokfulam, Hong Kong, SAR Hong Kong
    BMC Microbiol 4:9. 2004
    ..The corresponding pathway has been unravelled in Bacillus subtilis. However methylthioadenosine is subjected to alternative degradative pathways depending on the organism...
  3. ncbi request reprint Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon
    Kentaro Takahara
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Japan
    FEBS J 272:5353-64. 2005
    ..These findings suggest that CLGAT can effectively participate in the biosynthesis of citrulline in wild watermelon leaves during drought/strong-light stress...
  4. pmc Structural and functional similarities between a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-like protein from Bacillus subtilis and photosynthetic RuBisCO
    Yohtaro Saito
    Nara Institute of Science and Technology, Graduate School of Biological Sciences, Ikoma, Nara, Japan
    J Biol Chem 284:13256-64. 2009
    ..These results suggest that the amino acid residues utilized in the B. subtilis RLP enolase reaction are the same as those utilized in the RuBisCO RuBP enolization reaction...
  5. doi request reprint Responses of the photosynthetic electron transport system to excess light energy caused by water deficit in wild watermelon
    Satoko Sanda
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    Physiol Plant 142:247-64. 2011
    ..The qualitative changes in photosynthetic proteins may switch the photosynthetic electron transport from normal photosynthesis mode to stress-tolerance mode...
  6. ncbi request reprint Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon
    Kinya Akashi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara 630 0101, Japan
    Biochem Biophys Res Commun 323:72-8. 2004
    ..This is the first demonstration of a plant MT with antioxidant properties. The results suggest that CLMT2 induction contributes to the survival of wild watermelon under severe drought/high light stress conditions...
  7. ncbi request reprint Co-expression of cytochrome b561 and ascorbate oxidase in leaves of wild watermelon under drought and high light conditions
    Yoshihiko Nanasato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630 0192 Japan
    Plant Cell Physiol 46:1515-24. 2005
    ....
  8. doi request reprint An evolutionally conserved Lys122 is essential for function in Rhodospirillum rubrum bona fide RuBisCO and Bacillus subtilis RuBisCO-like protein
    Toshihiro Nakano
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Biochem Biophys Res Commun 392:212-6. 2010
    ..The K122M and K122E mutants of BsRLP failed to form a catalytic dimer. Our results suggest that the lysine residue is essential for function in both enzymes, although in each case, its role is likely distinct...
  9. pmc A phosphofructokinase B-type carbohydrate kinase family protein, NARA5, for massive expressions of plastid-encoded photosynthetic genes in Arabidopsis
    Taro Ogawa
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Plant Physiol 151:114-28. 2009
    ..Our results strongly suggest that NARA5 is indispensable for hyperexpression of photosynthetic genes encoded in the plastid genome, particularly rbcL...
  10. doi request reprint Dynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit
    Kinya Akashi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara 630 0192, Japan
    Planta 233:947-60. 2011
    ....
  11. ncbi request reprint Enzymatic characterization of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis
    Yohtaro Saito
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
    Biosci Biotechnol Biochem 71:2021-8. 2007
    ..7 kJ mol(-1). The enzyme, with a molecular mass of 76 kDa, was composed of two subunits. The equilibrium constant in the reversible isomerase reaction [MTRu-1-P]/[MTR-1-P] was 6. We discuss the possible reaction mechanism...
  12. ncbi request reprint Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway in Bacillus subtilis
    Hiroki Ashida
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Biosci Biotechnol Biochem 72:959-67. 2008
    ..048 s(-1) to an unknown compound that was not utilized by DK-MTP-1-P enolase, the enzyme catalyzing the next step. The function of this enzyme in the pathway is discussed...
  13. ncbi request reprint Characterization of Arabidopsis ZIM, a member of a novel plant-specific GATA factor gene family
    Masahito Shikata
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    J Exp Bot 55:631-9. 2004
    ..Genes in ZIM-overexpressing plants were identified that were up-regulated in a tissue-specific manner, which suggests that transcriptional regulation by ZIM and its consequent effects are spatially controlled...
  14. ncbi request reprint Molecular characterization of the cytoplasmic interacting protein of the receptor kinase IRK expressed in the inflorescence and root apices of Arabidopsis
    Junichiro Hattan
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Nara 630 0192, Japan
    Biosci Biotechnol Biochem 68:2598-606. 2004
    ..IRKI expression was expanded by exogenous auxin treatment and repressed by inhibitor treatment of polar auxin transport...
  15. doi request reprint A DEAD box protein is required for formation of a hidden break in Arabidopsis chloroplast 23S rRNA
    Kenji Nishimura
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
    Plant J 63:766-77. 2010
    ..We propose a molecular mechanism for the RH39-mediated fragmentation processing of 23S rRNA in chloroplasts...
  16. doi request reprint Programmed proteome response for drought avoidance/tolerance in the root of a C(3) xerophyte (wild watermelon) under water deficits
    Kazuya Yoshimura
    Nara Institute of Science and Technology, Graduate School of Biological Sciences, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Plant Cell Physiol 49:226-41. 2008
    ..This study provides new insights into the complex molecular networks within plant roots involved in the adaptation to adverse environments...
  17. doi request reprint The long-term responses of the photosynthetic proton circuit to drought
    Kaori Kohzuma
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
    Plant Cell Environ 32:209-19. 2009
    ....
  18. ncbi request reprint Plausible novel ribose metabolism catalyzed by enzymes of the methionine salvage pathway in Bacillus subtilis
    Toshihiro Nakano
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Takayama cho, Ikoma, Nara, Japan
    Biosci Biotechnol Biochem 77:1104-7. 2013
    ..9 µmol min(-1) mg of protein(-1) and 4.8 mM. Downstream enzymes catalyzed the isomerization of ribose-1-phosphate and subsequent dehydration, enolization, dephosphorylation, and dioxygenation...
  19. ncbi request reprint Potential involvement of N-terminal acetylation in the quantitative regulation of the ε subunit of chloroplast ATP synthase under drought stress
    Saki Hoshiyasu
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara, Japan
    Biosci Biotechnol Biochem 77:998-1007. 2013
    ..We propose a model in which quantitative regulation of the ε subunit involves N-terminal acetylation and stress-induced proteases...
  20. doi request reprint His267 is involved in carbamylation and catalysis of RuBisCO-like protein from Bacillus subtilis
    Toshihiro Nakano
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara 630 0192, Japan
    Biochem Biophys Res Commun 431:176-80. 2013
    ..The different functions of the active site histidine in RLP and RuBisCO are discussed...
  21. ncbi request reprint Continuous spectrophotometric assays for three regulatory enzymes of the arginine biosynthetic pathway
    Kentaro Takahara
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Anal Biochem 368:138-47. 2007
    ..These coupled enzyme assays are rapid, highly sensitive, and reproducible...
  22. doi request reprint RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway: functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO
    Hiroki Ashida
    Nara Institute of Science and Technology, Graduate School of Biological Sciences, 8916 5 Takayama, Ikoma, Nara, 630 0101 Japan
    J Exp Bot 59:1543-54. 2008
    ..In addition, the fundamental question, 'How has RuBisCO evolved?' is also considered, and evidence is presented that RuBisCOs evolved from RLPs...
  23. ncbi request reprint Arabidopsis ZIM, a plant-specific GATA factor, can function as a transcriptional activator
    Masahito Shikata
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan
    Biosci Biotechnol Biochem 67:2495-7. 2003
    ..ZIM functioned as a transcriptional activator, and the transactivation domain was found to occur in its N-terminal acidic region...
  24. ncbi request reprint Was photosynthetic RuBisCO recruited by acquisitive evolution from RuBisCO-like proteins involved in sulfur metabolism?
    Hiroki Ashida
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Res Microbiol 156:611-8. 2005
    ..In this mini-review, we suggest functional and evolutionary links between B. subtilis RLP and photosynthetic RuBisCO. Furthermore, we propose that photosynthetic RuBisCOs evolved from RLPs similar to that found in B. subtilis...
  25. ncbi request reprint Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli
    Keiko Mukougawa
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    FEBS Lett 580:1333-8. 2006
    ..coli cells accumulated orange-fluorescent phytochrome. The metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors...
  26. ncbi request reprint Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana
    Hideki Hyodo
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Plant Mol Biol 52:473-82. 2003
    ..These observations suggest that XTH9 expression is coordinated with plant development, including the differentiation from vegetative and reproductive meristems and cell elongation of the inflorescence stem...
  27. doi request reprint Establishment of a transgenic hairy root system in wild and domesticated watermelon (Citrullus lanatus) for studying root vigor under drought
    Masataka Kajikawa
    Nara Institute of Science and Technology, Graduate School of Biological Sciences, 8916 5 Takayama, Ikoma, Nara, 630 0192, Japan
    Plant Cell Rep 29:771-8. 2010
    ..The new protocol presented here offers a powerful tool for the comparative study of the molecular mechanism underlying drought-induced root growth in desert plants...
  28. doi request reprint Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species
    Yuri Nakajima Munekage
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, 630 0192 Japan
    Plant Cell Physiol 51:664-8. 2010
    ....
  29. pmc Complementation of phytochrome chromophore-deficient Arabidopsis by expression of phycocyanobilin:ferredoxin oxidoreductase
    Chitose Kami
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Proc Natl Acad Sci U S A 101:1099-104. 2004
    ....
  30. doi request reprint Production of biologically active human thioredoxin 1 protein in lettuce chloroplasts
    Soon Lim
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara 630 0192, Japan
    Plant Mol Biol 76:335-44. 2011
    ..This research opens up possibilities for plant-based production of hTrx1. Considering that this expression host is an edible crop plant, this transplastomic lettuce may be suitable for oral delivery of hTrx1...
  31. ncbi request reprint An Arabidopsis MADS-box protein, AGL24, is specifically bound to and phosphorylated by meristematic receptor-like kinase (MRLK)
    Hidetomo Fujita
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara, 630 0192 Japan
    Plant Cell Physiol 44:735-42. 2003
    ..These results suggest that MRLK signaling promotes translocation of AGL24 from the cytoplasm to the nucleus. We propose that the RLK signaling pathway involves phosphorylation of a MADS-box transcription factor...
  32. doi request reprint Promotion of cyclic electron transport around photosystem I during the evolution of NADP-malic enzyme-type C4 photosynthesis in the genus Flaveria
    Naoya Nakamura
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
    New Phytol 199:832-42. 2013
    ..We propose that these changes were important for the establishment of C4 photosynthesis from C3-C4 intermediate photosynthesis in Flaveria...
  33. ncbi request reprint A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO
    Hiroki Ashida
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Science 302:286-90. 2003
    ..Thus, the photosynthetic RuBisCO from R. rubrum retains the ability to function in the methionine salvage pathway in B. subtilis...
  34. pmc MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila
    Toshihiro Nakano
    Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara, Japan
    PLoS ONE 8:e67385. 2013
    ..Our results suggest that the MtnB domain of T. thermophila MtnBD acquired the new function to catalyze both the dehydratase and enolase reactions through evolutionary gene mutations, rather than fusion of MSP genes...
  35. ncbi request reprint Purification and characterization of class-I and class-II fructose-1,6-bisphosphate aldolases from the cyanobacterium Synechocystis sp. PCC 6803
    Ken Nakahara
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara, 630 0101 Japan
    Plant Cell Physiol 44:326-33. 2003
    ..PCC 6803 in the cleavage reaction of FBP were generally similar, except in their reactivity for SBP. The SBP/FBP activity ratio of the CII-FBA was two times higher than that of the CI-FBA...
  36. ncbi request reprint Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts
    Taise Shimaoka
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Eur J Biochem 270:921-8. 2003
    ..Site-directed mutagenesis indicated that C23 in DHAR is indispensable for the reduction of DHA. The mechanism of catalysis of spinach chloroplast DHAR is proposed...
  37. pmc Expression and biochemical properties of a ferredoxin-dependent heme oxygenase required for phytochrome chromophore synthesis
    Takuya Muramoto
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Plant Physiol 130:1958-66. 2002
    ..These results show that the basic mechanism of heme cleavage has been conserved between plants and other organisms even though the function, subcellular localization, and cofactor requirements of heme oxygenases differ substantially...
  38. ncbi request reprint [Multidimensional responses of plants to drought and strong light stresses]
    Kinya Akashi
    Tanpakushitsu Kakusan Koso 52:594-600. 2007
  39. ncbi request reprint Cyclic electron flow within PSII functions in intact chloroplasts from spinach leaves
    Chikahiro Miyake
    Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6 10 1, Hakozaki, Higashi ku, Fukuoka, 812 8581 Japan
    Plant Cell Physiol 43:951-7. 2002
    ....
  40. ncbi request reprint Physiological functions of the water-water cycle (Mehler reaction) and the cyclic electron flow around PSI in rice leaves
    Amane Makino
    Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori Amamiyamachi, Aoba ku, Sendai, 981 8555 Japan
    Plant Cell Physiol 43:1017-26. 2002
    ..However, WWC does not act to maintain a high NPQ, and PhiPSII is down-regulated by DeltapH generated via the cyclic electron flow around PSI...
  41. ncbi request reprint Irreversible cross-linking of heme to the distal tryptophan of stromal ascorbate peroxidase in response to rapid inactivation by H2O2
    Sakihito Kitajima
    Graduate School of Science and Technology, Kyoto Institute of Technology, Japan
    FEBS J 274:3013-20. 2007
    ..This is the first report of a mutation that improves the tolerance of chloroplast APXs to H2O2...
  42. ncbi request reprint Stable form of ascorbate peroxidase from the red alga Galdieria partita similar to both chloroplastic and cytosolic isoforms of higher plants
    Sakihito Kitajima
    Research institute of Innovative Technology for the Earth RITE, Soraku gun, Kyoto, 619 0292, Japan
    Biosci Biotechnol Biochem 66:2367-75. 2002
    ..The stability of APX-B might be due to its cytosolic isoform-like structure of the carboxyl-terminal half...
  43. ncbi request reprint A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis
    Alyssa Carré-Mlouka
    Departement de Microbiologie, Unité des Cyanobactéries CNRS URA 2172 and Plate forme Génomique Pasteur Génopole Ile de France, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Biol Chem 281:24462-71. 2006
    ..The discovery of the coexistence of RbcL(I) and RbcL(IV) in cyanobacteria, the ancestors of chloroplasts, enlightens episodes of the chaotic evolutionary history of the Rubiscos, a protein family of major importance for life on Earth...
  44. ncbi request reprint Chemical modification of arginine alleviates the decline in activity during catalysis of spinach Rubisco
    Eiichi Mizohata
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 301:591-7. 2003
    ..This is the first report identifying PhG-modified arginine residues and to demonstrate the effect of the modification of arginine residues on the kinetics of fallover...
  45. doi request reprint Molecular design of photosynthesis-elevated chloroplasts for mass accumulation of a foreign protein
    Yukinori Yabuta
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Plant Cell Physiol 49:375-85. 2008
    ..Consequently, >99% of the additional 200-260 mg ml(-1) of protein expressed in the chloroplasts could be used for the production of useful proteins in the photosynthesis-elevated transplastomic plants having FBP/SBPase...
  46. ncbi request reprint The rbcX gene product promotes the production and assembly of ribulose-1,5-bisphosphate carboxylase/oxygenase of Synechococcus sp. PCC7002 in Escherichia coli
    Takuo Onizuka
    Biological Science Laboratories, Toray Research Center, Inc, 1111 Tebiro, Kamakura, Kanagawa, 248 8555 Japan
    Plant Cell Physiol 45:1390-5. 2004
    ..The present rbcX coexpression system provides a novel and useful method for investigating the Rubisco maturation pathway...
  47. ncbi request reprint The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco
    Yusuke Kato
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, 606 8502 Kyoto, Japan
    Planta 220:97-104. 2004
    ..These results suggest that CND41 is involved in Rubisco degradation and the translocation of nitrogen during senescence. The possible regulation of protease activity of CND41 through DNA-binding is discussed...
  48. ncbi request reprint Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana
    Taise Shimaoka
    Department of Biological Science, Faculty of Science, Nara Women s University, 630 8506 Japan
    Plant Cell Physiol 45:672-83. 2004
    ..There were also a number of proteins for which a function has not yet been deduced...