Shinya Toyokuni

Summary

Affiliation: Nagoya University
Country: Japan

Publications

  1. doi request reprint Role of iron in carcinogenesis: cancer as a ferrotoxic disease
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Graduate School of Medicine, Nagoya University, Nagoya 466 8550, Japan
    Cancer Sci 100:9-16. 2009
  2. pmc Metamorphosis of mesothelial cells with active horizontal motility in tissue culture
    Hirotaka Nagai
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, 466 8550, Japan
    Sci Rep 3:1144. 2013
  3. pmc A novel method for efficient collection of normal mesothelial cells in vivo
    Li Jiang
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 46:265-8. 2010
  4. pmc A beverage containing fermented black soybean ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats
    Yasumasa Okazaki
    Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama 700 8558, Japan
    J Clin Biochem Nutr 47:198-207. 2010
  5. pmc Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats
    Yasumasa Okazaki
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Aichi 466 8550, Japan
    J Clin Biochem Nutr 51:84-90. 2012
  6. pmc Distinct affinity of nuclear proteins to the surface of chrysotile and crocidolite
    Yurika Kubo
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 51:221-6. 2012
  7. pmc Genome-scale approaches to investigate oxidative DNA damage
    Shinya Akatsuka
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 47:91-7. 2010
  8. pmc Age- and sun exposure-dependent differences in 8-hydroxy-2'-deoxyguanosine and N-(carboxymethyl)lysine in human epidermis
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 49:121-4. 2011
  9. pmc Mysterious link between iron overload and CDKN2A/2B
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya, Aichi 466 8550, Japan
    J Clin Biochem Nutr 48:46-9. 2011
  10. doi request reprint Genotoxicity and carcinogenicity risk of carbon nanotubes
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan Electronic address
    Adv Drug Deliv Rev 65:2098-110. 2013

Detail Information

Publications88

  1. doi request reprint Role of iron in carcinogenesis: cancer as a ferrotoxic disease
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Graduate School of Medicine, Nagoya University, Nagoya 466 8550, Japan
    Cancer Sci 100:9-16. 2009
    ..These results warrant reconsideration of the role of iron in carcinogenesis and suggest that fine control of body iron stores would be a wise strategy for cancer prevention...
  2. pmc Metamorphosis of mesothelial cells with active horizontal motility in tissue culture
    Hirotaka Nagai
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, 466 8550, Japan
    Sci Rep 3:1144. 2013
    ..Our evidence complements the current hypotheses of mesothelial regeneration and suggests that certain types of differentiated mesothelial cells undergo morphological changes before initiating migration to repair injured sites...
  3. pmc A novel method for efficient collection of normal mesothelial cells in vivo
    Li Jiang
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 46:265-8. 2010
    ..This simple method would be helpful for obtaining mesothelial control samples from animals to elucidate the molecular mechanisms of a variety of mesothelial pathology...
  4. pmc A beverage containing fermented black soybean ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats
    Yasumasa Okazaki
    Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Okayama 700 8558, Japan
    J Clin Biochem Nutr 47:198-207. 2010
    ..These data suggest that dietary intake of BBS is useful for the prevention of renal tubular oxidative damage mediate by iron, and warrant further investigation...
  5. pmc Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats
    Yasumasa Okazaki
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Aichi 466 8550, Japan
    J Clin Biochem Nutr 51:84-90. 2012
    ..We thus demonstrated the antioxidative effect of bLF against Fe-NTA-induced renal oxidative injury. These results suggest that LF intake is useful for the prevention of renal tubular oxidative damage mediated by iron...
  6. pmc Distinct affinity of nuclear proteins to the surface of chrysotile and crocidolite
    Yurika Kubo
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 51:221-6. 2012
    ..Our results provide novel evidence demonstrating that the specific molecular interactions leading to carcinogenesis are different between chrysotile and crocidolite...
  7. pmc Genome-scale approaches to investigate oxidative DNA damage
    Shinya Akatsuka
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 47:91-7. 2010
    ..The immunoprecipitation-based technique combined with high-density microarrays is one of the promising methods to provide access to such novel research strategies. We propose this sort of research area as oxygenomics...
  8. pmc Age- and sun exposure-dependent differences in 8-hydroxy-2'-deoxyguanosine and N-(carboxymethyl)lysine in human epidermis
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Clin Biochem Nutr 49:121-4. 2011
    ..Further investigation is warranted to elucidate underlying molecular mechanisms...
  9. pmc Mysterious link between iron overload and CDKN2A/2B
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya, Aichi 466 8550, Japan
    J Clin Biochem Nutr 48:46-9. 2011
    ..CDKN2A/2B are the second most frequently inactivated tumor suppressing genes in human cancers. Currently, when infection is becoming sufficiently controlled worldwide, iron regulation may be the next target for human longevity...
  10. doi request reprint Genotoxicity and carcinogenicity risk of carbon nanotubes
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan Electronic address
    Adv Drug Deliv Rev 65:2098-110. 2013
    ..CNTs still have a bright future with superb economic and medical merits. However, appropriate regulation of the production, distribution and secondary manufacturing processes is required, at least to protect the workers. ..
  11. ncbi request reprint Deferasirox induces mesenchymal-epithelial transition in crocidolite-induced mesothelial carcinogenesis in rats
    Hirotaka Nagai
    Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya, Aichi 466 8550, Japan
    Cancer Prev Res (Phila) 6:1222-30. 2013
    ..Still, our results suggest deferasirox serves as a potential preventive strategy in people already exposed to asbestos via iron reduction...
  12. doi request reprint Iron as a target of chemoprevention for longevity in humans
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    Free Radic Res 45:906-17. 2011
    ..Given that the required amounts of iron decrease during ageing, the fine control of body iron stores would be a wise strategy for chemoprevention of several diseases...
  13. ncbi request reprint Mechanisms of asbestos-induced carcinogenesis
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    Nagoya J Med Sci 71:1-10. 2009
    ..Elucidation of the major mechanisms underlying DMM would be helpful for the development of novel strategies to prevent DMM induction in people who have already been exposed to asbestos...
  14. ncbi request reprint [Mechanisms of asbestos-induced carcinogenesis]
    Shinya Toyokuni
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Nihon Eiseigaku Zasshi 66:562-7. 2011
    ..In this review, we summarize the cutting edge results of our 5-year project funded by a MEXT grant, in which local iron deposition and the characteristics of mesothelial cells are the key issues...
  15. doi request reprint Pluripotency of a single spermatogonial stem cell in mice
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 78:681-7. 2008
    ..Thus, our study provides evidence that a single spermatogonial stem cell can acquire pluripotentiality but that conversion into a pluripotent cell type is accompanied by loss of spermatogenic potential...
  16. doi request reprint Homozygous deletion of CDKN2A/2B is a hallmark of iron-induced high-grade rat mesothelioma
    Qian Hu
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Lab Invest 90:360-73. 2010
    ..These results not only show two distinct molecular pathways for iron-induced peritoneal mesothelioma, but also support the hypothesis that oxidative stress by iron overload is a major cause of CDKN2A/2B homozygous deletion...
  17. ncbi request reprint Deletion and single nucleotide substitution at G:C in the kidney of gpt delta transgenic mice after ferric nitrilotriacetate treatment
    Li Jiang
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cancer Sci 97:1159-67. 2006
    ..The results demonstrate that the iron-based Fenton reaction is mutagenic in vivo in the renal tubular cells and induces characteristic mutations...
  18. pmc Contrasting genome-wide distribution of 8-hydroxyguanine and acrolein-modified adenine during oxidative stress-induced renal carcinogenesis
    Shinya Akatsuka
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Am J Pathol 169:1328-42. 2006
    ..This versatile technique would introduce a novel research area as a high-throughput screening method for critical genomic loci under oxidative stress...
  19. ncbi request reprint Susceptibility of actin to modification by 4-hydroxy-2-nonenal
    Munetaka Ozeki
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 827:119-26. 2005
    ..The results suggest that actin is a major target protein for HNE modification in vivo, and that our monoclonal antibodies are useful for evaluating the HNE adducts produced...
  20. doi request reprint Iron overload signature in chrysotile-induced malignant mesothelioma
    Li Jiang
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Pathol 228:366-77. 2012
    ..Therefore, an intervention to remove local excess iron might be a strategy to prevent MM after asbestos exposure...
  21. ncbi request reprint Anchorage-independent growth of mouse male germline stem cells in vitro
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Biol Reprod 74:522-9. 2006
    ..These results indicate that spermatogonial stem cells can undergo anchorage-independent, self-renewal division, and suggest that stem cells have the common property to survive and proliferate in the absence of exogenous substrata...
  22. doi request reprint Stage-specific roles of fibulin-5 during oxidative stress-induced renal carcinogenesis in rats
    Hiroki Ohara
    Department of Pathology and Biological Responses, Graduate School of Medicine, Nagoya University, Showa Ku, Nagoya 466 8550, Japan
    Free Radic Res 45:211-20. 2011
    ..The data suggest that Fbln5 plays a role not only in the tissue repair and remodelling after renal tubular oxidative damage but also in RCC metastasis, presumably as a cytokine...
  23. ncbi request reprint Production of knockout mice by gene targeting in multipotent germline stem cells
    Masanori Takehashi
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Dev Biol 312:344-52. 2007
    ..Thus, mGS cells can be used to produce KO animals, and this novel method of germline manipulation may prove useful in diverse mammalian species...
  24. ncbi request reprint Association of microRNA-34a overexpression with proliferation is cell type-dependent
    Khokon Kumar Dutta
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cancer Sci 98:1845-52. 2007
    ....
  25. pmc Fenton reaction induced cancer in wild type rats recapitulates genomic alterations observed in human cancer
    Shinya Akatsuka
    Department of Pathology, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    PLoS ONE 7:e43403. 2012
    ..Based on the characteristics of extensive genome alterations in human cancer, our results suggest that this chemical reaction may play a major role during human carcinogenesis...
  26. doi request reprint Characteristics and modifying factors of asbestos-induced oxidative DNA damage
    Li Jiang
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Cancer Sci 99:2142-51. 2008
    ....
  27. ncbi request reprint Leukemia inhibitory factor enhances formation of germ cell colonies in neonatal mouse testis culture
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 76:55-62. 2007
    ..These results demonstrate that LIF is useful in the initiation of GS cell culture and suggest that LIF or a related cytokine is involved in the maturation of gonocytes into spermatogonia...
  28. ncbi request reprint Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 72:985-91. 2005
    ..This culture system will provide a new opportunity for understanding the regulatory mechanism that governs spermatogonial stem cells...
  29. pmc Diameter and rigidity of multiwalled carbon nanotubes are critical factors in mesothelial injury and carcinogenesis
    Hirotaka Nagai
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    Proc Natl Acad Sci U S A 108:E1330-8. 2011
    ..This work suggests that control of the diameter of MWCNTs could reduce the potential hazard to human health...
  30. doi request reprint Protective effect of cardioplegia with poly (ADP-ribose) polymerase-1 inhibitor against myocardial ischemia-reperfusion injury: in vitro study of isolated rat heart model
    Kazuhiro Yamazaki
    Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Enzyme Inhib Med Chem 28:143-7. 2013
    ..Thus, addition of 4-HQ in the cardioplegia may provide a new intervention for myocardial protection against ischemia-reperfusion injury by decreasing NAD(+) consumption and suppressing oxidative stress...
  31. pmc The circadian clock gene BMAL1 is a novel therapeutic target for malignant pleural mesothelioma
    Momen Elshazley
    Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Aichi, Japan
    Int J Cancer 131:2820-31. 2012
    ..Taken together, these findings indicate that BMAL1 has a critical role in MPM and could serve as an attractive therapeutic target for MPM...
  32. doi request reprint Asbestos surface provides a niche for oxidative modification
    Hirotaka Nagai
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Cancer Sci 102:2118-25. 2011
    ....
  33. ncbi request reprint Akt mediates self-renewal division of mouse spermatogonial stem cells
    Jiyoung Lee
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 134:1853-9. 2007
    ..These results demonstrate that activation of the PI3K-Akt pathway plays a central role in the self-renewal division of spermatogonial stem cells...
  34. ncbi request reprint Alpha-tocopherol induces calnexin in renal tubular cells: another protective mechanism against free radical-induced cellular damage
    Wen Hua Lee
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Arch Biochem Biophys 453:168-78. 2006
    ..This adds another molecular mechanism of alpha-tocopherol toward cancer prevention...
  35. pmc Adenovirus-mediated gene delivery into mouse spermatogonial stem cells
    Masanori Takehashi
    Department of Molecular Genetics, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 104:2596-601. 2007
    ..Nevertheless, these results suggest that adenovirus may inadvertently integrate into the patient's germ line and indicate that there is no barrier to adenovirus infection in spermatogonial stem cells...
  36. ncbi request reprint Genetic and epigenetic properties of mouse male germline stem cells during long-term culture
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 132:4155-63. 2005
    ....
  37. ncbi request reprint Protective effect of colored rice over white rice on Fenton reaction-based renal lipid peroxidation in rats
    Shinya Toyokuni
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Japan
    Free Radic Res 36:583-92. 2002
    ..These may partly explain the antioxidative effect. Furthermore, colored strains of rice are rich in proteins. Thus, our data warrants further investigation of the antioxidative effect of colored rice...
  38. doi request reprint Cancer-promoting role of adipocytes in asbestos-induced mesothelial carcinogenesis through dysregulated adipocytokine production
    Shan Hwu Chew
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    Carcinogenesis 35:164-72. 2014
    ..Taken together, our findings support a potential cancer-promoting role of adipocytes in asbestos-induced mesothelial carcinogenesis. ..
  39. ncbi request reprint Formation of acrolein-derived 2'-deoxyadenosine adduct in an iron-induced carcinogenesis model
    Yoshichika Kawai
    Laboratory of Food and Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    J Biol Chem 278:50346-54. 2003
    ..The data obtained in this study suggest that the formation of a carcinogenic aldehyde through lipid peroxidation may be causally involved in the pathophysiological effects associated with oxidative stress...
  40. pmc Chronic oxidative stress causes amplification and overexpression of ptprz1 protein tyrosine phosphatase to activate beta-catenin pathway
    Yu Ting Liu
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Am J Pathol 171:1978-88. 2007
    ..Thus, iron-mediated persistent oxidative stress confers an environment for gene amplification...
  41. doi request reprint In vitro transformation of mouse testis cells by oncogene transfection
    Hiroko Morimoto
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 86:148, 1-11. 2012
    ..Thus, in vitro testis cell transformation facilitates an analysis of the GCT formation process, and our results also suggest the close similarity between GCT formation and reprogramming...
  42. doi request reprint Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation
    Jiyoung Lee
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 5:76-86. 2009
    ..Thus, the Ras-cyclin D2 pathway regulates the balance between tissue maintenance and tumorigenesis in the SSC population...
  43. doi request reprint Genome-wide analysis identifies a tumor suppressor role for aminoacylase 1 in iron-induced rat renal cell carcinoma
    Yi Zhong
    Department of Pathology and Biological Responses, Graduate School of Medicine, Nagoya University, Nagoya, Japan
    Carcinogenesis 30:158-64. 2009
    ..Thus, Fe-NTA-induced renal tumor model is ideal for studying the link between deletions within this region and tumor formation. Our data demonstrate that Acy1 functions as a tumor suppressor in this rat RCC model...
  44. pmc Lipid peroxidation generates body odor component trans-2-nonenal covalently bound to protein in vivo
    Kousuke Ishino
    Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    J Biol Chem 285:15302-13. 2010
    ....
  45. doi request reprint Protein N-acylation: H2O2-mediated covalent modification of protein by lipid peroxidation-derived saturated aldehydes
    Kousuke Ishino
    Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    Chem Res Toxicol 21:1261-70. 2008
    ..These data suggest that the hydroperoxides, including H2O2, might be involved not only in the oxidative modification of protein but also in the covalent binding of the saturated aldehydes to proteins under oxidative stress...
  46. ncbi request reprint Novel surrogate end-point biomarker to evaluate agents for use in the chemoprevention of reactive oxygen species-associated cancer
    Makoto Hiroyasu
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Redox Rep 7:335-8. 2002
    ..This novel method is versatile, and may work as a surrogate end-point biomarker for screening the usefulness of agents for cancer chemoprevention...
  47. ncbi request reprint Regulation of mouse spermatogonial stem cell self-renewing division by the pituitary gland
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Japan
    Biol Reprod 70:1731-7. 2004
    ..Thus, the pituitary gland affects the self-renewing division of stem cells, but these contradictory results suggest that its role may be different depending on the stage of the testicular development...
  48. ncbi request reprint Generation of pluripotent stem cells from neonatal mouse testis
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell 119:1001-12. 2004
    ..The ability to derive multipotential stem cells from the neonatal testis has important implications for germ cell biology and opens the possibility of using these cells for biotechnology and medicine...
  49. ncbi request reprint Effects of the phenolic contents of Mauritian endemic plant extracts on promoter activities of antioxidant enzymes
    Shinya Toyokuni
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Free Radic Res 37:1215-24. 2003
    ..These results suggest that the chemopreventive potentials of the extracts might reside in their abilities to modulate the expression of the antioxidant enzyme genes...
  50. doi request reprint Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects
    Seiji Takashima
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 81:155-64. 2009
    ..Although genetic manipulation did not increase the conversion of GS cells to a pluripotent state, these results underscore the important role of DNMTs in survival and spermatogenic differentiation in SSCs...
  51. ncbi request reprint Molecular mechanisms of oxidative stress-induced carcinogenesis: from epidemiology to oxygenomics
    Shinya Toyokuni
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    IUBMB Life 60:441-7. 2008
    ..Thus, possible underlying principles should be elucidated in association with each cell type, the genomic location of the damage with its transcriptional activity as well as the chromatin status determining the epigenetic effect...
  52. ncbi request reprint Redox regulation of annexin 2 and its implications for oxidative stress-induced renal carcinogenesis and metastasis
    Tomoyuki Tanaka
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Oncogene 23:3980-9. 2004
    ..These results suggest that Anx2 is regulated by redox status, and that persistent operation of this adaptive mechanism plays a role in the proliferation and metastasis of oxidative stress-induced cancer...
  53. ncbi request reprint Expression of chromobox homolog 7 (CBX7) is associated with poor prognosis in ovarian clear cell adenocarcinoma via TRAIL-induced apoptotic pathway regulation
    Kanako Shinjo
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Int J Cancer 135:308-18. 2014
    ..We also successfully demonstrated that the TRAIL pathway is a novel target for CBX7 expression modulation in these cells, and therapeutic agents utilizing the TRAIL pathway may be particularly effective for targeted OCCA therapy. ..
  54. ncbi request reprint Prevention of myocardial reperfusion injury by poly(ADP-ribose) synthetase inhibitor, 3-aminobenzamide, in cardioplegic solution: in vitro study of isolated rat heart model
    Kazuhiro Yamazaki
    Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawahara machi, Sakyo ku, 606 8507, Kyoto, Japan
    Eur J Cardiothorac Surg 26:270-5. 2004
    ..We investigated the efficacy of a cardioplegic solution containing a PARS inhibitor, 3-aminobenzamide (3-AB), for myocardial protection against ischemia-reperfusion injury caused by cardioplegic arrest...
  55. ncbi request reprint Amelioration of cisplatin toxicity by a fermented grain food product
    Yukiko Minamiyama
    Department of Food and Health Science, Graduate School of Medicine and Dentistry, Okayama University, Shikatacho, Okayama 700 8558, Japan
    Biofactors 16:105-15. 2002
    ..The AOB effect was dose dependent. In conclusion, the present study suggests that certain food additives like AOB may be of benefit against the side effects of cisplatin...
  56. pmc Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells
    Mihoko Horio
    Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Ann Surg Oncol 19:S634-45. 2012
    ..The role of ZEB1, a master epithelial-to-mesenchymal transition gene, in malignant pleural mesothelioma (MPM) is unclear...
  57. doi request reprint The ferroimmunomodulatory role of ectopic endometriotic stromal cells in ovarian endometriosis
    Hiroharu Kobayashi
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Fertil Steril 98:415-22.e1-12. 2012
    ..To understand the role of ectopic endometriotic stromal cells in ovarian endometriosis (OEM) and the associated risks for infertility and carcinogenesis...
  58. ncbi request reprint Genetic selection of mouse male germline stem cells in vitro: offspring from single stem cells
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 72:236-40. 2005
    ....
  59. ncbi request reprint Two distinct mechanisms for loss of thioredoxin-binding protein-2 in oxidative stress-induced renal carcinogenesis
    Khokon K Dutta
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Lab Invest 85:798-807. 2005
    ....
  60. pmc Met is the most frequently amplified gene in endometriosis-associated ovarian clear cell adenocarcinoma and correlates with worsened prognosis
    Yoriko Yamashita
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan
    PLoS ONE 8:e57724. 2013
    ..Thus, we believe that targeted inhibition of the Met pathway may be a promising treatment for OCC...
  61. doi request reprint FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation
    Kei Ishii
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 139:1734-43. 2012
    ..These results show that FGF2 depends on MAP2K1 signaling to drive SSC self-renewal via upregulation of the Etv5 and Bcl6b genes...
  62. doi request reprint Contents of endometriotic cysts, especially the high concentration of free iron, are a possible cause of carcinogenesis in the cysts through the iron-induced persistent oxidative stress
    Ken Yamaguchi
    Department of Gynecology and Obstetrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Clin Cancer Res 14:32-40. 2008
    ....
  63. doi request reprint Effect of edaravone, a novel free radical scavenger, supplemented to cardioplegia on myocardial function after cardioplegic arrest: in vitro study of isolated rat heart
    Kazuhiro Yamazaki
    Department of Cardiovascular Surgery, Graduate School of Medicine, 54 Shogoin, Kawahara machi, Sakyo ku, Kyoto 606 8507, Japan
    Heart Vessels 24:228-35. 2009
    ..In this study, the maximum improvement in the myocardial function was achieved by addition of a moderate dose (10 microM) of edaravone...
  64. ncbi request reprint Transgenic mice produced by retroviral transduction of male germ line stem cells in vivo
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University Yoshida Konoe, Kyoto, Japan
    Biol Reprod 71:1202-7. 2004
    ....
  65. ncbi request reprint Iron and carcinogenesis: from Fenton reaction to target genes
    Shinya Toyokuni
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Redox Rep 7:189-97. 2002
    ..Here, recent new findings on iron metabolism are reviewed and the concept of the vulnerable sites explored. More effort to link iron metabolism with human carcinogenesis is anticipated...
  66. doi request reprint CD146 and insulin-like growth factor 2 mRNA-binding protein 3 predict prognosis of asbestos-induced rat mesothelioma
    Yasumasa Okazaki
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Aichi, Japan
    Cancer Sci 104:989-95. 2013
    ..Furthermore, the overexpression of CD146 and/or IMP3 was proportionally correlated with shortened survival. These results suggest that CD146 and IMP3 are useful diagnostic and prognostic markers of MM. ..
  67. ncbi request reprint Helicobacter felis-induced gastritis was suppressed in mice overexpressing thioredoxin-1
    Kimio Kawasaki
    Department of Gastroenterology and Endoscopic Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Lab Invest 85:1104-17. 2005
    ..The present study suggests that overexpression of TRX-1 suppresses H. felis-induced gastritis by inhibiting chemotaxis of neutrophils and reducing oxidative stress...
  68. ncbi request reprint Transcriptional control of fetal liver hematopoiesis: dominant negative effect of the overexpression of the LIM domain mutants of LMO2
    Tomo Terano
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto, Japan
    Exp Hematol 33:641-51. 2005
    ..To elucidate the functional role of two LIM domains in LMO2, we introduced deletion or mutation in each of the LIM domains and analyzed their phenotypic effects on the hematopoietic system when overexpressed in vivo or in vitro...
  69. ncbi request reprint Granulocyte colony-stimulating factor protects cardiac mitochondria in the early phase of cardiac injury
    Yoshimi Hiraumi
    Dept of Pediatrics, Graduate School of Medicine, Kyoto Univ, Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Am J Physiol Heart Circ Physiol 296:H823-32. 2009
    ..These findings clearly indicate that G-CSF protects cardiac mitochondria, which are key organelles in the determination of cardiac cellular fate, in the early phase of cardiac injury...
  70. doi request reprint miR-375 is activated by ASH1 and inhibits YAP1 in a lineage-dependent manner in lung cancer
    Eri Nishikawa
    Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Cancer Res 71:6165-73. 2011
    ..Our results elucidate an ASH1 effector axis in NE-lung cancers that is functionally pivotal in controlling NE features and the alleviation from YAP1-mediated growth inhibition...
  71. ncbi request reprint Novel aspects of oxidative stress-associated carcinogenesis
    Shinya Toyokuni
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Japan
    Antioxid Redox Signal 8:1373-7. 2006
    ..Reliable markers for oxidative stress as well as for oxidative stress-induced preneoplastic lesions must be established...
  72. ncbi request reprint Peroxynitrite-mediated stress is associated with proliferation of human metastatic colorectal carcinoma in the liver
    Shohei Kondo
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Cancer Lett 179:87-93. 2002
    ..Our results suggest that contact of metastatic tumor induces apoptosis in adjacent hepatocytes through peroxynitrite, thus permitting the proliferation of cancer cells...
  73. doi request reprint Biopersistent fiber-induced inflammation and carcinogenesis: lessons learned from asbestos toward safety of fibrous nanomaterials
    Hirotaka Nagai
    Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Aichi, Japan
    Arch Biochem Biophys 502:1-7. 2010
    ..Since fibrous nanomaterials present potential applications in various fields, it is necessary to develop standard evaluation methods to minimize risks for human health...
  74. ncbi request reprint CD9 is a surface marker on mouse and rat male germline stem cells
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 70:70-5. 2004
    ....
  75. ncbi request reprint Spaciotemporal alteration of 8-hydroxy-2'-deoxyguanosine levels in cardiomyocytes after myocardial infarction in rats
    Senri Miwa
    Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, Japan
    Free Radic Res 36:853-8. 2002
    ..The present data support the use of supplementary antioxidant therapies to save functional myocardium after MI. (213 words)..
  76. ncbi request reprint Pathological investigation of oxidative stress in the post-genomic era
    Shinya Toyokuni
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Pathol Int 57:461-73. 2007
    ..These fruitful results may lead to more accurate and useful pathological diagnosis and more efficient prophylaxis and therapeutic interventions on human diseases...
  77. doi request reprint XCR1 expression and biased VH gene usage are distinct features of diffuse large B-cell lymphoma initially manifesting in the bone marrow
    Yoriko Yamashita
    Dept of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Japan
    Am J Clin Pathol 135:556-64. 2011
    ..05), which was confirmed by quantitative reverse transcriptase-PCR and immunohistochemical analysis. Expression of the chemokine receptor XCR1 and frequent usage of autoreactive V(H) genes seem to be distinct characteristics of BM-DLBCL...
  78. doi request reprint LATS2 is a tumor suppressor gene of malignant mesothelioma
    Hideki Murakami
    Division of Molecular Oncology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Cancer Res 71:873-83. 2011
    ..Thus, our results suggest that the inactivation of LATS2 is one of the key mechanisms for constitutive activation of YAP, which induces deregulation of MM cell proliferation...
  79. ncbi request reprint Detection of glyceraldehyde 3-phosphate dehydrogenase messenger RNA using a peptide nucleic acid probe in paraffin-embedded archival specimens
    Makoto Hiroyasu
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Pathol Int 54:251-5. 2004
    ..Oligonucleotide PNA probe, albeit at high cost, would increase the value of paraffin-embedded specimens in storage for use in human medical research...
  80. pmc Overexpression of inducible cyclic AMP early repressor inhibits transactivation of genes and cell proliferation in pancreatic beta cells
    Akari Inada
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Mol Cell Biol 24:2831-41. 2004
    ..These results suggest that CRE and CRE-binding factors have an important role in pancreatic beta-cell physiology not only directly by regulation of gene trans-activation but also indirectly by regulation of beta-cell mass...
  81. ncbi request reprint Isolation and characterization of annexin 2 pseudogene in Rattus norvegicus
    Munetaka Ozeki
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Gene 289:185-90. 2002
    ..In the present study, we demonstrated the presence of an annexin 2 pseudogene in rats...
  82. doi request reprint Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis
    Yu Ting Liu
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto, 606 8501, Japan
    Methods Mol Biol 536:307-12. 2009
    ..With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years...
  83. ncbi request reprint Structural basis of protein-bound endogenous aldehydes. Chemical and immunochemical characterizations of configurational isomers of a 4-hydroxy-2-nonenal-histidine adduct
    Mika Hashimoto
    Laboratory of Food and Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    J Biol Chem 278:5044-51. 2003
    ....
  84. ncbi request reprint Glycine ameliorates lung reperfusion injury after cold preservation in an ex vivo rat lung model
    Mitsugu Omasa
    Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Transplantation 75:591-8. 2003
    ..The role of glycine has not been investigated in lung ischemia-reperfusion injury after cold preservation. Furthermore, the role of apoptosis after reperfusion following cold preservation has not been fully understood...
  85. pmc Specific allelic loss of p16 (INK4A) tumor suppressor gene after weeks of iron-mediated oxidative damage during rat renal carcinogenesis
    Makoto Hiroyasu
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Am J Pathol 160:419-24. 2002
    ..Therefore, the p16 (INK4A) locus is specifically vulnerable to oxidative damage, leading to its allelic loss within weeks, presumably due to a deficiency in the replication of both the alleles...
  86. ncbi request reprint Heat shock preconditioning reduces the formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal modified proteins in ischemia-reperfused liver of rats
    Kazuhiko Yamagami
    Department of Gastroenterological Surgery, Kyoto University, Graduate School of Medicine, Japan
    Free Radic Res 36:169-76. 2002
    ..As determined by levels of 8-OHdG and HNE-modified proteins produced during ischemia-reperfusion of the liver, HSPC reduced the oxidative injury of cellular proteins and DNA in the liver tissue...
  87. ncbi request reprint Thioredoxin as a biomarker for oxidative stress in patients with rheumatoid arthritis
    Takumi Jikimoto
    Department of Clinical Pathology and Immunology, Graduate School of Medicine, Kobe University, 7 5 2 Kusunoki cho, Chuou ku, Kobe, 650 0017, Hyogo, Japan
    Mol Immunol 38:765-72. 2002
    ..005). We concluded that plasma TRX level is a new biomarker for the disease activity of RA and may reflect higher levels of oxidative stress in RA patients...