Gohta Goshima

Summary

Affiliation: Nagoya University
Country: Japan

Publications

  1. pmc Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    J Cell Biol 181:421-9. 2008
  2. doi request reprint New look inside the spindle: microtubule-dependent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Curr Opin Cell Biol 22:44-9. 2010
  3. pmc The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells
    Ryota Uehara
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 106:6998-7003. 2009
  4. pmc An inducible RNA interference system in Physcomitrella patens reveals a dominant role of augmin in phragmoplast microtubule generation
    Yuki Nakaoka
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Plant Cell 24:1478-93. 2012
  5. pmc Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin
    Wenjing Li
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Biol 199:849-62. 2012
  6. pmc Identification of a TPX2-like microtubule-associated protein in Drosophila
    Gohta Goshima
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya, Japan
    PLoS ONE 6:e28120. 2011
  7. pmc Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase
    Ryota Uehara
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Biol 202:623-36. 2013
  8. pmc Augmin-dependent microtubule nucleation at microtubule walls in the spindle
    Tomoko Kamasaki
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Cell Biol 202:25-33. 2013
  9. pmc Endogenous localizome identifies 43 mitotic kinesins in a plant cell
    Tomohiro Miki
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 111:E1053-61. 2014
  10. pmc EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells
    Wenjing Li
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8601, Japan
    J Cell Biol 193:973-83. 2011

Collaborators

Detail Information

Publications27

  1. pmc Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    J Cell Biol 181:421-9. 2008
    ..Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules...
  2. doi request reprint New look inside the spindle: microtubule-dependent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Curr Opin Cell Biol 22:44-9. 2010
    ..RNA interference (RNAi) screens with quantitative image analysis led to the identification of the augmin complex that plays a key role in this MT generation process...
  3. pmc The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells
    Ryota Uehara
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 106:6998-7003. 2009
    ..These results demonstrate that MT generation mediated by augmin and gamma-TuRC is critical for chromosome segregation and cytokinesis in human cells...
  4. pmc An inducible RNA interference system in Physcomitrella patens reveals a dominant role of augmin in phragmoplast microtubule generation
    Yuki Nakaoka
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Plant Cell 24:1478-93. 2012
    ....
  5. pmc Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin
    Wenjing Li
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Biol 199:849-62. 2012
    ..We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215(msps) polymerase and the EB1-Sentin duo...
  6. pmc Identification of a TPX2-like microtubule-associated protein in Drosophila
    Gohta Goshima
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya, Japan
    PLoS ONE 6:e28120. 2011
    ..Thus, while this conserved spindle protein is present in Drosophila, other mechanisms may largely compensate for its spindle assembly and chromosome segregation functions...
  7. pmc Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase
    Ryota Uehara
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Biol 202:623-36. 2013
    ..On the basis of these results, we propose a robust self-organization mechanism for central spindle formation. ..
  8. pmc Augmin-dependent microtubule nucleation at microtubule walls in the spindle
    Tomoko Kamasaki
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Cell Biol 202:25-33. 2013
    ..Furthermore, they suggest a mechanism for maintaining polarized MT organization, even when noncentrosomal MT initiation is widespread...
  9. pmc Endogenous localizome identifies 43 mitotic kinesins in a plant cell
    Tomohiro Miki
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 111:E1053-61. 2014
    ..Furthermore, our data challenge the current generalization of determining mitotic kinesin function based solely on studies using yeast and animal cells. ..
  10. pmc EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells
    Wenjing Li
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8601, Japan
    J Cell Biol 193:973-83. 2011
    ..These results indicate that EB1 promotes dynamic microtubule behavior by recruiting the cargo protein Sentin and possibly also a microtubule polymerase to the microtubule tip...
  11. pmc Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase
    Ryota Uehara
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Biol 191:259-67. 2010
    ..Based on these results, a new model for central spindle assembly is proposed...
  12. doi request reprint Control of mitotic spindle length
    Gohta Goshima
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Annu Rev Cell Dev Biol 26:21-57. 2010
    ..Here we review molecules and mechanisms that control spindle length, how these mechanisms are deployed in different systems, and some quantitative models that describe the control of spindle length...
  13. doi request reprint Assessment of mitotic spindle phenotypes in Drosophila S2 cells
    Gohta Goshima
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Methods Cell Biol 97:259-75. 2010
    ..Here, we discuss how bona fide mitotic phenotypes associated with RNAi can be identified, avoiding selecting false positives, in S2 cells...
  14. pmc Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
    Yi Guo
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Nature 453:657-61. 2008
    ..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
  15. pmc Genes required for mitotic spindle assembly in Drosophila S2 cells
    Gohta Goshima
    Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA
    Science 316:417-21. 2007
    ..The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled...
  16. pmc Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway
    Gohta Goshima
    COE Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
    J Cell Biol 160:25-39. 2003
    ..RNAi for hMis6, like that of a kinetochore kinesin CENP-E, induces mitotic arrest. Kinetochore localization of hMis12 is unaffected by CENP-A RNAi, demonstrating an independent pathway of CENP-A in human kinetochores...
  17. pmc The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line
    Gohta Goshima
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1003-16. 2003
    ..From our phenotypic data, we construct a model for the distinct roles of molecular motors during mitosis in a single metazoan cell type...
  18. pmc Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94107, USA
    Mol Biol Cell 16:3896-907. 2005
    ..These results reveal a diverse spectrum of regulatory mechanisms for controlling the localization and function of five mitotic kinesins at different stages of the cell cycle...
  19. pmc Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 171:229-40. 2005
    ..From these results and simulations, we propose a model on how two minus end-directed motors cooperate to ensure spindle pole coalescence during mitosis...
  20. ncbi request reprint Length control of the metaphase spindle
    Gohta Goshima
    Physiology Course 2004, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Curr Biol 15:1979-88. 2005
    ..Although a number of molecular perturbations have been shown to influence spindle length, a global understanding of the factors that determine metaphase spindle length has not been achieved...
  21. ncbi request reprint Making microtubules and mitotic spindles in cells without functional centrosomes
    Nicole M Mahoney
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94107, USA
    Curr Biol 16:564-9. 2006
    ..We propose that the bipolar spindle propagates its own architecture by stimulating microtubule growth, thereby augmenting the well-described microtubule nucleation pathways that take place at centrosomes and chromosomes...
  22. ncbi request reprint CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation
    Keita Aoki
    Core Research for Evolutional Science and Technology Research Program of Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Curr Biol 16:1627-35. 2006
    ..Cdc2 thus directly phosphorylates Dis1, and this phosphorylation regulates Dis1 localization in both metaphase and anaphase and ensures high-fidelity segregation...
  23. ncbi request reprint Regulation of mitochondria distribution by RhoA and formins
    Alexander A Minin
    Institute of Protein Research, Russian Academy of Sciences, Moscow 119988, Russia
    J Cell Sci 119:659-70. 2006
    ..Thus, mitochondrial movement is controlled by the small GTPase RhoA and this control is mediated by formins...
  24. ncbi request reprint A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1
    Chikashi Obuse
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nat Cell Biol 6:1135-41. 2004
    ..Double HP1 RNAi abolishes kinetochore localization of hMis12 and DC8. Therefore, centromeric HP1 may be the base to anchor the hMis12 core complex that is enriched with coiled coils and extends to outer Zwint-1 during mitosis...
  25. ncbi request reprint [Roles of kinesins in mitosis]
    Gohta Goshima
    Tanpakushitsu Kakusan Koso 51:579-85. 2006
  26. pmc The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore
    Gohta Goshima
    COE Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502 Japan
    EMBO J 22:2752-63. 2003
    ..Ppe1/PP6 directly or indirectly modulates kinetochore chromatin protein Mis12 to ensure progression into normal anaphase...
  27. ncbi request reprint Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast
    Yusuke Toyoda
    Department of Gene Mechanisms, Graduate School of Biostudies, 606 8502, Kyoto, Japan
    Curr Biol 12:347-58. 2002
    ..The failure to form cohesion in the S phase results in premature separation of chromatids in G2/M. Mitotic kinetochores free from microtubules or the lack of tension are known to activate spindle checkpoint...