Y Ishimi

Summary

Affiliation: Mitsubishi Kagaku Institute of Life Sciences
Country: Japan

Publications

  1. ncbi request reprint Biochemical function of mouse minichromosome maintenance 2 protein
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 273:8369-75. 1998
  2. ncbi request reprint Phosphorylation of Mcm4 at specific sites by cyclin-dependent kinase leads to loss of Mcm4,6,7 helicase activity
    Y Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 276:34428-33. 2001
  3. ncbi request reprint A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 272:24508-13. 1997
  4. ncbi request reprint Biochemical activities associated with mouse Mcm2 protein
    Y Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 276:42744-52. 2001
  5. ncbi request reprint Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix
    Yukio Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, Japan
    Eur J Biochem 270:1089-101. 2003
  6. ncbi request reprint Levels of MCM4 phosphorylation and DNA synthesis in DNA replication block checkpoint control
    Yukio Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Struct Biol 146:234-41. 2004
  7. ncbi request reprint Identification of MCM4 as a target of the DNA replication block checkpoint system
    Yukio Ishimi
    Biomolecular and Technology Department, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 278:24644-50. 2003
  8. ncbi request reprint Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 275:16235-41. 2000
  9. ncbi request reprint Binding of human minichromosome maintenance proteins with histone H3
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 271:24115-22. 1996
  10. pmc Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity
    Z You
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194 8511, Japan
    Mol Cell Biol 19:8003-15. 1999

Detail Information

Publications72

  1. ncbi request reprint Biochemical function of mouse minichromosome maintenance 2 protein
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 273:8369-75. 1998
    ..These results suggest that MCM2 plays a different role in the initiation of DNA replication than the other MCM proteins...
  2. ncbi request reprint Phosphorylation of Mcm4 at specific sites by cyclin-dependent kinase leads to loss of Mcm4,6,7 helicase activity
    Y Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 276:34428-33. 2001
    ..These results suggest that phosphorylation of Mcm4 at specific sites leads to loss of Mcm4,6,7 DNA helicase activity...
  3. ncbi request reprint A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 272:24508-13. 1997
    ..These results suggest that this helicase may be involved in the initiation of DNA replication as a DNA unwinding enzyme...
  4. ncbi request reprint Biochemical activities associated with mouse Mcm2 protein
    Y Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 276:42744-52. 2001
    ..Finally, we identified a region required for the nuclear localization of Mcm2. The function of Mcm2 is discussed based on these biochemical characteristics...
  5. ncbi request reprint Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix
    Yukio Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, Japan
    Eur J Biochem 270:1089-101. 2003
    ....
  6. ncbi request reprint Levels of MCM4 phosphorylation and DNA synthesis in DNA replication block checkpoint control
    Yukio Ishimi
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Struct Biol 146:234-41. 2004
    ..These results are consistent with the notion that the phosphorylation of MCM4 is involved in regulation of DNA synthesis in the checkpoint control...
  7. ncbi request reprint Identification of MCM4 as a target of the DNA replication block checkpoint system
    Yukio Ishimi
    Biomolecular and Technology Department, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 278:24644-50. 2003
    ....
  8. ncbi request reprint Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    J Biol Chem 275:16235-41. 2000
    ..These results raise the possibility that the inactivation of Mcm4,6,7 helicase activity by Cdk2 is a part of the system for regulating DNA replication...
  9. ncbi request reprint Binding of human minichromosome maintenance proteins with histone H3
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 271:24115-22. 1996
    ..Immunoprecipitation with anti-Cdc21 antibody revealed that these four MCM proteins form complexes. These results are consistent with the findings that MCM proteins bind with chromatin in vivo...
  10. pmc Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity
    Z You
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194 8511, Japan
    Mol Cell Biol 19:8003-15. 1999
    ..The results also indicated that the two activities of DNA helicase and single-stranded DNA binding can be separated...
  11. ncbi request reprint Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    J Biol Chem 266:7025-9. 1991
    ..Proteins recognized by the anti-NAP-I antibody were also present in Xenopus oocytes and Drosophila cultured cells. Possible roles of NAP-I are discussed in relation to other nucleosome assembly proteins...
  12. ncbi request reprint Selective effects of genistein, a soybean isoflavone, on B-lymphopoiesis and bone loss caused by estrogen deficiency
    Y Ishimi
    Department of Food Science, The National Institute of Health and Nutrition, Tokyo, Japan
    Endocrinology 140:1893-900. 1999
    ..Phytoestrogens may be useful for preventing bone loss caused by estrogen deficiency in females...
  13. ncbi request reprint Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity
    T Fujii-Nakata
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    J Biol Chem 267:20980-6. 1992
    ..This minimal size fragment could form the 12 S NAP-1-histone complex as the whole protein could, whereas deleted fragments on either side could bind with core histones only to form aggregates...
  14. ncbi request reprint Difference in effective dosage of genistein on bone and uterus in ovariectomized mice
    Y Ishimi
    Division of Food Science, National Institute of Health and Nutrition, Tokyo, 162 8636, Japan
    Biochem Biophys Res Commun 274:697-701. 2000
    ..3 nmol/ml) and 50-fold (20.4 nmol/ml) higher than that in OVX mice. These results suggest that there is a marked difference between genistein dosages that protect against bone loss and those that induce uterine hypertrophy...
  15. ncbi request reprint Promoter region of mouse Tcrg genes
    Y Ishimi
    Dept of Immunology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    Immunogenetics 43:68-71. 1996
  16. ncbi request reprint The specific production of the third component of complement by osteoblastic cells treated with 1 alpha,25-dihydroxyvitamin D3
    T Sato
    Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan
    FEBS Lett 285:21-4. 1991
    ....
  17. ncbi request reprint Growth regulation of the expression of mouse cDNA and gene encoding a serine/threonine kinase related to Saccharomyces cerevisiae CDC7 essential for G1/S transition. Structure, chromosomal localization, and expression of mouse gene for s. cerevisiae Cdc7-
    J M Kim
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 273:23248-57. 1998
    ..Transient transfection assays indicated that a 231-base pair segment of the muCdc7 promoter containing three putative E2F binding sites and one Sp1 site but lacking TATA sequence is sufficient for response to growth stimulation...
  18. ncbi request reprint Distinct NFAT family proteins are involved in the nuclear NFAT-DNA binding complexes from human thymocyte subsets
    Y Amasaki
    Department of Cell Signaling, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA
    J Immunol 160:2324-33. 1998
    ..These results indicate that NFAT family members are differentially regulated during the development of T cells, and that NFATx may play a distinct role in calcineurin-dependent signaling in DP thymocytes...
  19. ncbi request reprint Molecular cloning and functional characterization of murine cDNA encoding transcription factor NFATc
    S Pan
    Department of Molecular and Developmental Biology, University of Tokyo, Japan
    Biochem Biophys Res Commun 240:314-23. 1997
    ..In the mouse embryo, mNFATc transcript was strongly expressed in thymus, lung and submandibular gland and weakly in skeletal muscle and heart suggesting that mNFATc may have a role both in embryogenesis and in mature T cells...
  20. ncbi request reprint Four P-like elements are required for optimal transcription of the mouse IL-4 gene: involvement of a distinct set of nuclear factor of activated T cells and activator protein-1 family proteins
    N Takemoto
    Department of Stem Cell Regulation, Institute of Medical Science, University of Tokyo, Japan
    Int Immunol 9:1329-38. 1997
    ..It would thus appear that components of each P-like element-binding complexes are not identical and may coordinately contribute to transcriptional activity...
  21. pmc Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
    N Sato
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    EMBO J 16:4340-51. 1997
    ..The wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro, suggesting that huCdc7 may regulate processes of DNA replication by modulating MCM functions...
  22. ncbi request reprint Carboxyl-terminal 15-amino acid sequence of NFATx1 is possibly created by tissue-specific splicing and is essential for transactivation activity in T cells
    R Imamura
    Department of Cell Signaling, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304 1104, USA
    J Immunol 161:3455-63. 1998
    ..These data demonstrate that the 15-amino acid sequence of NFATx1 is a major transactivation sequence required for induction of genes by NFATx1 in T cells and possibly regulates NFAT activity through tissue-specific alternative splicing...
  23. pmc A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells
    H Kumagai
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Japan
    Mol Cell Biol 19:5083-95. 1999
    ..Our data show that ASK is a novel cyclin-like regulatory subunit of the huCdc7 kinase complex and that it plays a pivotal role in G1/S transition in mammalian cells...
  24. ncbi request reprint Cell cycle-dependent interaction of Mad2 with conserved Box1/2 region of human granulocyte-macrophage colony-stimulating factor receptor common betac
    M Takeda
    Department of Molecular and Developmental Biology, Institute of Medical Science, Core Research for Evolutional Science and Technology, Japan
    J Biol Chem 276:41803-9. 2001
    ..Binding affinity of Mad2 to box1/2 increased in the late M phase, suggesting the possibility that GM-CSF participates in regulation of the M phase check point through interaction with Mad2...
  25. ncbi request reprint Sna41goa1, a novel mutation causing G1/S arrest in fission yeast, is defective in a CDC45 homolog and interacts genetically with polalpha
    M Uchiyama
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Japan
    Mol Genet Genomics 265:1039-49. 2001
    ..Our genetic results are consistent with a model in which Cdc45 plays crucial roles in the assembly of the replication apparatus at replication origins...
  26. ncbi request reprint Inhibition of NFATx activation by an oligopeptide: disrupting the interaction of NFATx with calcineurin
    J Liu
    Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA
    J Immunol 167:2677-87. 2001
    ..This approach may provide an opportunity for pharmacological targeting of Ca(2+)-dependent signaling events...
  27. ncbi request reprint Bipartite binding of a kinase activator activates Cdc7-related kinase essential for S phase
    K Ogino
    Department of Molecular and Developmental Biology, Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, CREST, Japan
    J Biol Chem 276:31376-87. 2001
    ..Dbf4-related kinase complexes at the origins, in which they are tethered to replication machinery through Dbf4 motif N and the catalytic subunits are activated through bipartite binding of Dbf4 motifs M and C of the regulatory subunits...
  28. ncbi request reprint Essential role of Sna41/Cdc45 in loading of DNA polymerase alpha onto minichromosome maintenance proteins in fission yeast
    M Uchiyama
    Department of Molecular and Developmental Biology, Institute for Medical Science, The University of Tokyo, 4 6 1 Shirokanedai Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 276:26189-96. 2001
    ....
  29. ncbi request reprint Recruitment of nonexpanded polyglutamine proteins to intranuclear aggregates in neuronal intranuclear hyaline inclusion disease
    J Takahashi
    Division of Neuropathology, The Jikei University School of Medicine, Tokyo, Japan
    J Neuropathol Exp Neurol 60:369-76. 2001
    ..This hypothesis is supported by the finding that the polyglutamine epitope in the center of NIs was surrounded by ubiquitin...
  30. ncbi request reprint In vitro analysis of STAT5 activation by granulocyte-macrophage colony-stimulating factor
    Y Sakurai
    Department of Molecular and Developmental Biology, Institute of Medical Science, Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, University of Tokyo
    Genes Cells 5:937-947. 2000
    ..Biochemical studies suggested that signalling pathways are transmitted through protein/protein interactions, but how these biochemical cascades are initiated and transmitted in response to cytokine stimulation is largely unknown...
  31. ncbi request reprint NFATz: a novel rel similarity domain containing protein
    S Pan
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Japan
    Biochem Biophys Res Commun 272:765-76. 2000
    ..The chromosomal location determined by FISH revealed that NFATz and NFATx genes are in the same region...
  32. ncbi request reprint Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 bY Cdks
    H Masai
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, CREST, Japan
    J Biol Chem 275:29042-52. 2000
    ..In vitro, Cdk2-Cyclin E, Cdk2-Cyclin A, and Cdc2-Cyclin B, but not Cdk4-Cyclin D1, phosphorylates the Thr-376 residue of huCdc7, suggesting possible regulation of huCdc7 by Cdks...
  33. pmc A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage
    T Takeda
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Mol Cell Biol 19:5535-47. 1999
    ..Our results reveal a novel function of the Cdc7/Dbf4-related kinase complex in S-phase checkpoint control as well as in growth recovery from DNA damage in addition to its predicted essential function in S-phase initiation...
  34. ncbi request reprint Analyses of Saccharomyces cerevisiae Cdc7 kinase point mutants: dominant-negative inhibition of DNA replication on overexpression of kinase-negative Cdc7 proteins
    A Ohtoshi
    Department of Molecular and Developmental Biology, Institute of Medical Science, The University of Tokyo, Minato ku, Japan
    Mol Gen Genet 254:562-70. 1997
    ..Our results are consistent with the notion that association of Dbf4 with Cdc7 is essential for the G1 to S transition in S. cerevisiae...
  35. ncbi request reprint Mechanism of dnaB protein action. III. Allosteric role of ATP in the alteration of DNA structure by dnaB protein in priming replication
    K Arai
    J Biol Chem 256:5260-6. 1981
  36. ncbi request reprint Complexes of Rep protein with ATP and DNA as a basis for helicase action
    N Arai
    J Biol Chem 256:5287-93. 1981
    ..These and related studies serve as guides to understanding the multiple interactions of rep protein with its ATP and DNA ligands that enable it to unwind duplex DNA at a replication fork...
  37. ncbi request reprint Definition of cis-regulatory elements of the mouse interleukin-5 gene promoter. Involvement of nuclear factor of activated T cell-related factors in interleukin-5 expression
    H J Lee
    Department of Molecular and Developmental Biology, University of Tokyo, Japan
    J Biol Chem 270:17541-50. 1995
    ..It thus seems likely that an NF-AT-related factor is involved in the regulation of IL-5 gene transcription...
  38. pmc NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus
    E S Masuda
    Department of Cell Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304, USA
    Mol Cell Biol 15:2697-706. 1995
    ..The expression pattern of NFATx, together with its functional activity, strongly suggests that NFATx plays a role in the regulation of gene expression in T cells and immature thymocytes...
  39. ncbi request reprint The transcription factor Sp1 is required for induction of the murine GM-CSF promoter in T cells
    E S Masuda
    Department of Cellular Biology, DNAX Research Institute, Palo Alto, CA 94304
    Biochem Biophys Res Commun 205:1518-25. 1994
    ..Since the human GM-CSF promoter has a base difference within the GC-box, we speculate that this may explain why the human promoter is weak and that an upstream enhancer is required for the induction of the human GM-CSF gene...
  40. ncbi request reprint Mechanism of dnaB protein action. II. ATP hydrolysis by dnaB protein dependent on single- or double-stranded DNA
    K Arai
    J Biol Chem 256:5253-9. 1981
  41. pmc Calcineurin-dependent nuclear translocation of a murine transcription factor NFATx: molecular cloning and functional characterization
    J Liu
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Japan
    Mol Biol Cell 8:157-70. 1997
    ..These results provide the first evidence for the requirement of calcineurin in the control of mNFATx imported from the cytoplasm to the nucleus and implies that mNFATx may possibly be a substrate of calcineurin in vivo...
  42. ncbi request reprint Genetic interactions between CDC7 and CDC28: growth inhibition of cdc28-1N by Cdc7 point mutants
    A Ohtoshi
    Department of Molecular and Developmental Biology, Institute of Medical Science, The University of Tokyo, Japan
    Genes Cells 1:895-904. 1996
    ..This is caused by titration of a regulatory protein, Dbf4, from the wild-type Cdc7, which leads to inactivation of its kinase activity...
  43. pmc RepA protein- and oriR-dependent initiation of R1 plasmid replication: identification of a rho-dependent transcription terminator required for cis-action of repA protein
    H Masai
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
    Nucleic Acids Res 16:6493-514. 1988
    ..A model which may account for the role of CIS in mediating the cis-action of the repA protein is presented...
  44. ncbi request reprint Initiation of lagging-strand synthesis for pBR322 plasmid DNA replication in vitro is dependent on primosomal protein i encoded by dnaT
    H Masai
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304
    J Biol Chem 263:15016-23. 1988
    ....
  45. ncbi request reprint Arginine as an exacerbating factor for glomerulonephritis in rats fed a methionine-threonine-supplemented low casein diet
    T Nihei
    Department of Applied Biological Science, Tokyo Noko University, Fuchu, Japan
    Biosci Biotechnol Biochem 65:1155-62. 2001
    ..These results suggest that the supplementation of L-arginine makes exacerbation via nitric oxide production in glomerulonephritis...
  46. ncbi request reprint Structure and properties of calphobindin II, an anticoagulant protein from human placenta
    H Yoshizaki
    Tokyo Research Laboratories, Kowa Co, Ltd
    J Biochem 107:43-50. 1990
    ..Calcium-binding studies indicated that 1 mol of CPB II binds 1 mol of calcium in the absence of phospholipid and 8 mol of calcium in the presence of phospholipid...
  47. pmc Reconstitution of functional receptors for human granulocyte/macrophage colony-stimulating factor (GM-CSF): evidence that the protein encoded by the AIC2B cDNA is a subunit of the murine GM-CSF receptor
    T Kitamura
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
    Proc Natl Acad Sci U S A 88:5082-6. 1991
    ..These results indicate that the beta subunit of the GM-CSF receptor is required for generation of growth signals and that AIC2B is likely the beta subunit of the mouse GM-CSF receptor...
  48. ncbi request reprint The inv(11)(p15q22) chromosome translocation of de novo and therapy-related myeloid malignancies results in fusion of the nucleoporin gene, NUP98, with the putative RNA helicase gene, DDX10
    Y Arai
    Radiobiology Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Blood 89:3936-44. 1997
    ..The predicted NUP98-DDX10 fusion protein may promote leukemogenesis through aberrant nucleoplasmic transport of mRNA or alterations in ribosome assembly...
  49. ncbi request reprint AIC2A is a component of the purified high affinity mouse IL-3 receptor: temperature-dependent modulation of AIC2A structure
    J Schreurs
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
    Int Immunol 3:1231-42. 1991
    ....
  50. ncbi request reprint Characterization of isotype-specific regions of five classes of canine beta-tubulin and their expression in several tissues and cell culture
    K Arai
    Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Fuchu, Japan
    J Vet Med Sci 63:1297-302. 2001
    ..In vitro, taxol-resistant sublines displayed a significant increase in class IVa as compared with taxol-sensitive cells, suggesting that altered expression of class IVa was associated with taxol resistance in these cell lines...
  51. ncbi request reprint Frequent allelic loss/imbalance on the short arm of chromosome 3 in tongue cancer
    K Arai
    First Department of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan
    Bull Tokyo Dent Coll 42:151-7. 2001
    ..Our data support the notion that tumor suppressor gene(s) contributing to the progression of tongue squamous cell carcinoma reside on 3p24 and 3p25...
  52. ncbi request reprint Analysis of a role for p16/CDKN2 expression and methylation patterns in human oral squamous cell carcinoma
    T Yakushiji
    First Department of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan
    Bull Tokyo Dent Coll 42:159-68. 2001
    ..When we compared these results with clinicopathological stages, there was no statistical significance. These findings suggest that hypermethylation of p16/CDKN2 promoter region may be associated with p16/CDKN2 gene alteration...
  53. pmc Cloning and expression of a gene encoding an interleukin 3 receptor-like protein: identification of another member of the cytokine receptor gene family
    D M Gorman
    DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
    Proc Natl Acad Sci U S A 87:5459-63. 1990
    ..We found that the AIC2B gene was coexpressed with the AIC2A gene. These results suggest a potential involvement of AIC2B in cytokine signal transduction...
  54. ncbi request reprint Calreticulin is present in the acrosome of spermatids of rat testis
    M Nakamura
    Department of Obstetrics and Gynecology, School of Medicine, Teikyo University, Tokyo, Japan
    Biochem Biophys Res Commun 186:668-73. 1992
    ..On the immunoblot analysis, the purified rat CalBP reacted with an antibody raised against rabbit skeletal muscle calreticulin. The results indicate that calreticulin is present in the acrosome of spermatids of rat testes...
  55. ncbi request reprint The carboxyl terminus of RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function
    K P Garrett
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104
    J Biol Chem 267:23942-9. 1992
    ..2, is able to assemble with the beta (RAP74) subunit to form a mutant beta gamma (RAP30/74) with impaired ability to interact with RNA polymerase II...
  56. ncbi request reprint Analysis of cartilage oligomeric matrix protein (COMP) degradation and synthesis in equine joint disease
    K Arai
    Department of Veterinary Medicine, Kagoshima University, 21 24 Korimoto 1 chome, Kagoshima 890 0065, Japan
    Equine Vet J 37:31-6. 2005
    ....
  57. ncbi request reprint Structure and expression of cDNA for an inhibitor of blood coagulation isolated from human placenta: a new lipocortin-like protein
    A Iwasaki
    Department of Cell Biology, Kowa Research Institute, Ibaraki
    J Biochem 102:1261-73. 1987
    ..The resulting recombinant protein manifested inhibitory activities against both blood coagulation and phospholipase A2 activity, as did the coagulation inhibitor isolated from human placenta...
  58. ncbi request reprint Clathrin box in G protein-coupled receptor kinase 2
    T Shiina
    Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    J Biol Chem 276:33019-26. 2001
    ..Taken together, these results imply that GRK2 contains a functional clathrin box and directly interacts with clathrin to modulate its function...
  59. ncbi request reprint Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif
    T Toki
    Department of Pediatrics, Hirosaki University, Japan
    Oncogene 14:1901-10. 1997
    ..The results suggest that the small Maf family proteins function in human cells through interaction with various basic-leucine zipper-type transcription factors...
  60. pmc The beta subunit of human granulocyte-macrophage colony-stimulating factor receptor forms a homodimer and is activated via association with the alpha subunit
    A Muto
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Japan
    J Exp Med 183:1911-6. 1996
    ..These results indicate that the beta homodimer, which alone is insufficient for signaling, forms the functional hGMR with the alpha subunit in response to hGM-CSF...
  61. ncbi request reprint Structure of the gene encoding the alpha subunit of the human granulocyte-macrophage colony stimulating factor receptor. Implications for the evolution of the cytokine receptor superfamily
    Y Nakagawa
    Department of Molecular and Developmental Biology, University of Tokyo, Japan
    J Biol Chem 269:10905-12. 1994
    ..On the basis of these observations, we propose a model for evolution of this gene family...
  62. pmc Molecular cloning of a novel human cDNA encoding a zinc finger protein that binds to the interleukin-3 promoter
    N Koyano-Nakagawa
    Department of Molecular and Development Biology, Institute of Medical Science, University of Tokyo, Japan
    Mol Cell Biol 14:5099-107. 1994
    ..These results suggest that EGR1 has a role in inducible transcription of the IL-3 gene, while DB1 sustains basal transcriptional activity and also cooperates with Tax to activate the IL-3 promoter...
  63. ncbi request reprint Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes
    F Morohoshi
    Radiobiology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Gene 221:191-8. 1998
    ....
  64. ncbi request reprint cAMP activates the IL-5 promoter synergistically with phorbol ester through the signaling pathway involving protein kinase A in mouse thymoma line EL-4
    H J Lee
    Department of Molecular and Developmental Biology, University of Tokyo, Japan
    J Immunol 151:6135-42. 1993
    ..These results indicate that the IL-5 gene in EL-4 cells is positively regulated by cAMP in a manner opposite that for the IL-2 gene...
  65. pmc Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replication
    H Masai
    Department of Molecular and Developmental Biology, University of Tokyo, Japan
    EMBO J 13:5338-45. 1994
    ..abstract truncated at 250 words)..
  66. pmc Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells
    T Yokota
    Proc Natl Acad Sci U S A 81:1070-4. 1984
    ....
  67. ncbi request reprint Cloning of an interleukin-3 receptor gene: a member of a distinct receptor gene family
    N Itoh
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
    Science 247:324-7. 1990
    ..A sequence comparison of the IL-3 receptor with other cytokine receptors (erythropoietin, IL-4, IL-6, and the beta chain IL-2 receptor) revealed a common motif of a distinct receptor gene family...
  68. ncbi request reprint Mechanism of dnaB protein action. I. Crystallization and properties of dnaB protein, an essential replication protein in Escherichia coli
    K Arai
    J Biol Chem 256:5247-52. 1981
    ..Based on a native Mr = 290,000 and cross-linking studies that yielded six bands, dnaB protein is judged to be a hexamer, confirming the results of Reha-Krantz, L. J., and Hurwitz, J. (1978) J. Biol. Chem. 253, 4043-4050...
  69. pmc Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells
    F Lee
    Proc Natl Acad Sci U S A 82:4360-4. 1985
    ..These results demonstrate that identification of full-length cDNAs for many colony-stimulating factors may be achieved entirely on the basis of detecting the functional polypeptide produced in mammalian cells...
  70. pmc Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells
    T Yokota
    Proc Natl Acad Sci U S A 82:68-72. 1985
    ..These results demonstrate that identification of full-length cDNA clones for many lymphokines may be achieved entirely on the basis of detection of the functional polypeptides in mammalian cells...
  71. pmc Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases
    S W Wong
    Department of Pathology, Stanford Medical School, Stanford University, CA 94305
    EMBO J 7:37-47. 1988
    ..Three of these conserved regions appear to comprise the functional active site required for deoxynucleotide interaction. Two putative DNA interacting domains are also identified...
  72. ncbi request reprint Urine cartilage oligomeric matrix protein (COMP) measurement is useful in discriminating the osteoarthritic Thoroughbreds
    K Misumi
    Department of Veterinary Clinical Science, Kagoshima University, Kagoshima, Japan
    Osteoarthritis Cartilage 14:1174-80. 2006
    ..To quantify the urinary concentration of cartilage oligomeric matrix protein (COMP), and to evaluate the relationship between urinary COMP concentration and the catabolic activity of synovial fluid (SF) in diseased horses...