Affiliation: Mitsubishi Kagaku Institute of Life Sciences
Ishimi Y. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. J Biol Chem. 1997;272:24508-13 pubmed
..These results suggest that this helicase may be involved in the initiation of DNA replication as a DNA unwinding enzyme. ..
Ishimi Y, Komamura Kohno Y. Phosphorylation of Mcm4 at specific sites by cyclin-dependent kinase leads to loss of Mcm4,6,7 helicase activity. J Biol Chem. 2001;276:34428-33 pubmed
..These results suggest that phosphorylation of Mcm4 at specific sites leads to loss of Mcm4,6,7 DNA helicase activity. ..
Ishimi Y, Komamura Kohno Y, Arai K, Masai H. Biochemical activities associated with mouse Mcm2 protein. J Biol Chem. 2001;276:42744-52 pubmed
..Finally, we identified a region required for the nuclear localization of Mcm2. The function of Mcm2 is discussed based on these biochemical characteristics. ..
Ishimi Y, Komamura Kohno Y, Kwon H, Yamada K, Nakanishi M. Identification of MCM4 as a target of the DNA replication block checkpoint system. J Biol Chem. 2003;278:24644-50 pubmed
Ishimi Y, Komamura Kohno Y, Karasawa Shimizu K, Yamada K. Levels of MCM4 phosphorylation and DNA synthesis in DNA replication block checkpoint control. J Struct Biol. 2004;146:234-41 pubmed
..These results are consistent with the notion that the phosphorylation of MCM4 is involved in regulation of DNA synthesis in the checkpoint control. ..