Takashi Sugita


Affiliation: Meiji Pharmaceutical University
Country: Japan


  1. Higashide S, Cho O, Matsuda Y, Ogishima D, Kurakado S, Sugita T. Rapid detection of Lactobacillus crispatus and Lactobacillus iners in vaginal specimens by loop-mediated isothermal amplification. J Microbiol Methods. 2019;158:18-20 pubmed publisher
    ..The LAMP assay had a lower limit of detection of 10 fg DNA and could detect both species within 45 min. ..
  2. Sugita T, Yamazaki T, Yamada S, Takeoka H, Cho O, Tanaka T, et al. Temporal changes in the skin Malassezia microbiota of members of the Japanese Antarctic Research Expedition (JARE): A case study in Antarctica as a pseudo-space environment. Med Mycol. 2015;53:717-24 pubmed publisher
    ..restricta shifted to seborrheic dermatitis/dandruff types. These results provide useful information for the development of skin hygiene management plans for astronauts staying at the ISS. ..
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    Sugita T, Nishikawa A, Ichikawa T, Ikeda R, Shinoda T. Isolation of Trichosporon asahii from environmental materials. Med Mycol. 2000;38:27-30 pubmed
    ..We isolated 29 T. asahii colonies from environmental samples using the polymerase chain reaction (PCR) and dichloran rose bengal chloramphenicol (DRBC) medium. Our results suggest that T. asahii is common in nature. ..
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    Sugita T, Tajima M, Amaya M, Tsuboi R, Nishikawa A. Genotype analysis of Malassezia restricta as the major cutaneous flora in patients with atopic dermatitis and healthy subjects. Microbiol Immunol. 2004;48:755-9 pubmed
    ..These findings suggest that a specific genotype of M. restricta plays a significant role in AD, although M. restricta commonly colonizes both AD patients and healthy subjects. ..
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    Sugita T, Tajima M, Tsubuku H, Tsuboi R, Nishikawa A. Quantitative analysis of cutaneous malassezia in atopic dermatitis patients using real-time PCR. Microbiol Immunol. 2006;50:549-52 pubmed
    ..Seven to 12 times more Malassezia colonized the head and neck compared to the trunk or limbs, and the species M. globosa and M. restricta accounted for approximately 80% of all Malassezia colonization at any body site. ..
  6. Sugita T, Suzuki M, Goto S, Nishikawa A, Hiruma M, Yamazaki T, et al. Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay. Med Mycol. 2010;48:229-33 pubmed publisher
    ..M. globosa and M. restricta together accounted for more than 70% of Malassezia spp. recovered regardless of gender. The total colonization of Malassezia and the ratio of the two major species change with age and gender in humans. ..
  7. Sugita T, Kikuchi K, Makimura K, Urata K, Someya T, Kamei K, et al. Trichosporon species isolated from guano samples obtained from bat-inhabited caves in Japan. Appl Environ Microbiol. 2005;71:7626-9 pubmed
    ..Our findings suggest that Trichosporon spp. are the major yeast species in bat guano in Japan and that bat guano is a potentially rich source of previously undescribed yeast species. ..
  8. Sugita T, Ikeda R, Nishikawa A. Analysis of Trichosporon isolates obtained from the houses of patients with summer-type hypersensitivity pneumonitis. J Clin Microbiol. 2004;42:5467-71 pubmed
    ..Based on our analysis, we conclude that the strains that play the most significant roles in the development of SHP are T. dermatis, T. asahii genotype 3, and T. montevideense, representing serotypes I, II, and III, respectively. ..
  9. Sugita T, Ichikawa T, Matsukura M, Sueda M, Takashima M, Ikeda R, et al. Genetic diversity and biochemical characteristics of Trichosporon asahii isolated from clinical specimens, houses of patients with summer-type-hypersensitivity pneumonitis, and environmental materials. J Clin Microbiol. 2001;39:2405-11 pubmed
    ..asahii seem to be related to the source of the isolate. We also found a specific DNA fragment for the clinical isolates and strains isolated from SHP patients' houses. ..

More Information


  1. Sugita T, Nakajima M, Ikeda R, Matsushima T, Shinoda T. Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of Trichosporon species. J Clin Microbiol. 2002;40:1826-30 pubmed
    ..Our results suggest that analysis of IGS regions provides a powerful method to distinguish between phylogenetically closely related species and that a geographic substructure may exist among T. asahii clinical isolates. ..
  2. Cho O, Matsukura M, Sugita T. Molecular evidence that the opportunistic fungal pathogen Trichosporon asahii is part of the normal fungal microbiota of the human gut based on rRNA genotyping. Int J Infect Dis. 2015;39:87-8 pubmed publisher
    ..asahii were almost identical to those of reported clinical isolates. Therefore, T. asahii colonizing the gastrointestinal tract might be associated with the development of trichosporonosis, and not environmental isolates. ..
  3. Matsuda Y, Cho O, Sugita T, Ogishima D, Takeda S. Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells. Mycopathologia. 2018;183:691-700 pubmed publisher
    ..albicans adhesion to HeLa cells. These findings support the notion that Lactobacillus metabolites may be useful alternatives to antifungal drugs for the management of VVC. ..
  4. Sugita T, Tajima M, Ito T, Saito M, Tsuboi R, Nishikawa A. Antifungal activities of tacrolimus and azole agents against the eleven currently accepted Malassezia species. J Clin Microbiol. 2005;43:2824-9 pubmed
    ..245 to 0.378. Our results provide the basis for testing these agents in future clinical trials to reduce the number of Malassezia cells colonizing the skin surface in patients with AD. ..
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    Sugita T, Shiraki Y, Hiruma M. Real-time PCR TaqMan assay for detecting Trichophyton tonsurans, a causative agent of tinea capitis, from hairbrushes. Med Mycol. 2006;44:579-81 pubmed
    ..We recently developed a real-time PCR TaqMan assay as a culture-independent method for the rapid detection of T. tonsurans from hairbrushes. ..
  6. Sugita T, Yamazaki T, Makimura K, Cho O, Yamada S, Ohshima H, et al. Comprehensive analysis of the skin fungal microbiota of astronauts during a half-year stay at the International Space Station. Med Mycol. 2016;54:232-9 pubmed publisher
    ..These findings will provide information useful for maintaining the health of astronauts staying in the space environment for long periods and for preventing infection due to the human skin microbiota. ..
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    Cho O, Sugita T. Low DNA Sequence Diversity of the Intergenic Spacer 1 Region in the Human Skin Commensal Fungi Malassezia sympodialis and M. dermatis Isolated from Patients with Malassezia-Associated Skin Diseases and Healthy Subjects. Mycopathologia. 2016;181:839-842 pubmed
    ..Thus, given the lack of intraspecies diversity in the IGS regions of M. sympodialis and M. dermatis, studies of the diversity of these species should be performed using appropriate genes and not the IGS. ..
  8. Cho O, Ichikawa T, Kurakado S, Takashima M, Manabe R, Ohkuma M, et al. Draft Genome Sequence of the Causative Antigen of Summer-Type Hypersensitivity Pneumonitis, Trichosporon domesticum JCM 9580. Genome Announc. 2016;4: pubmed publisher
    ..This genomic information will help elucidate the mechanisms of the development of SHP. ..
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    Sugita T, Tajima M, Takashima M, Amaya M, Saito M, Tsuboi R, et al. A new yeast, Malassezia yamatoensis, isolated from a patient with seborrheic dermatitis, and its distribution in patients and healthy subjects. Microbiol Immunol. 2004;48:579-83 pubmed
    ..M. yamatoensis DNA was detected from 3 of 31 SD patients (9.7%), 5 of 36 AD patients (13.9%), and 1 of 22 healthy subjects (4.6%). Therefore, M. yamatoensis is a rare member of the cutaneous microflora...
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    Sugita T, Takashima M, Poonwan N, Mekha N, Malaithao K, Thungmuthasawat B, et al. The first isolation of ustilaginomycetous anamorphic yeasts, Pseudozyma species, from patients' blood and a description of two new species: P. parantarctica and P. thailandica. Microbiol Immunol. 2003;47:183-90 pubmed
    ..thailandica was also resistant to fluconazole and itraconazole. As far as we know, this is the first isolation of Pseudozyma strains from humans. The two new species are described...