Akihiro Yoshida

Summary

Affiliation: Kyushu Dental College
Country: Japan

Publications

  1. pmc Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis
    Akihiro Yoshida
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    BMC Infect Dis 12:253. 2012
  2. ncbi request reprint Multiplex polymerase chain reaction assay for simultaneous detection of black-pigmented Prevotella species in oral specimens
    A Yoshida
    Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu, Japan
    Oral Microbiol Immunol 20:43-6. 2005
  3. pmc LuxS-based signaling affects Streptococcus mutans biofilm formation
    Akihiro Yoshida
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu 803 8540, Japan
    Appl Environ Microbiol 71:2372-80. 2005
  4. pmc Loop-mediated isothermal amplification method for rapid detection of the periodontopathic bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
    Akihiro Yoshida
    Division of Community Oral Health Science, Kyushu Dental College, 2 6 1 Manazuru, Kitakyushu 803 8580, Japan
    J Clin Microbiol 43:2418-24. 2005
  5. doi request reprint Hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria
    Akihiro Yoshida
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    J Periodontol 80:1845-51. 2009
  6. ncbi request reprint Quantitative detection of volatile sulfur compound- producing microorganisms in oral specimens using real-time PCR
    H Kato
    Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu, Japan
    Oral Dis 11:67-71. 2005
  7. doi request reprint Oral health in patients on haemodialysis for diabetic nephropathy and chronic glomerulonephritis
    Gou Teratani
    Division of Community Oral Health Science, Department of Health Promotion, School of Dentistry, Kyushu Dental College, 2 6 1 Manazuru, Kokurakita ku, Kitakyushu 803 8580, Japan
    Clin Oral Investig 17:483-9. 2013
  8. doi request reprint Does decreased diastolic blood pressure associate with increased mortality in 80-year-old Japanese?
    Shuntaro Kagiyama
    Division of General Internal Medicine, Department of Health Promotion, Science of Health Improvement, Kyushu Dental College, Kitakyushu, Japan
    Clin Exp Hypertens 31:639-47. 2009
  9. doi request reprint Association of cortisol and dehydroepiandrosterone sulphate levels in serum with periodontal status in older Japanese adults
    Aiko Ishisaka
    Department of Health Promotion, Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    J Clin Periodontol 35:853-61. 2008
  10. ncbi request reprint Serum albumin levels as an independent predictor of 4-year mortality in a community-dwelling 80-year-old population
    Yutaka Takata
    Division of General Internal Medicine, Kyushu Dental College, Kitakyushu, 803 8580, Japan
    Aging Clin Exp Res 22:31-5. 2010

Collaborators

Detail Information

Publications62

  1. pmc Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis
    Akihiro Yoshida
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    BMC Infect Dis 12:253. 2012
    ..However, quantitative detection based on qPCR for the JP2 clone has not been developed due to genetic difficulties. In this study, we developed a qPCR-based quantification method specific to the JP2 clone...
  2. ncbi request reprint Multiplex polymerase chain reaction assay for simultaneous detection of black-pigmented Prevotella species in oral specimens
    A Yoshida
    Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu, Japan
    Oral Microbiol Immunol 20:43-6. 2005
    ..This assay will be useful for determining the prevalence of these organisms in the oral ecosystem. Furthermore, this assay system should prove a useful tool for analyzing the role of black-pigmented Prevotella species in the mouth...
  3. pmc LuxS-based signaling affects Streptococcus mutans biofilm formation
    Akihiro Yoshida
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu 803 8540, Japan
    Appl Environ Microbiol 71:2372-80. 2005
    ..Furthermore, these results provide further confirmation of previous proposals that quorum sensing via AI-2 may play a significant role in oral biofilm formation...
  4. pmc Loop-mediated isothermal amplification method for rapid detection of the periodontopathic bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
    Akihiro Yoshida
    Division of Community Oral Health Science, Kyushu Dental College, 2 6 1 Manazuru, Kitakyushu 803 8580, Japan
    J Clin Microbiol 43:2418-24. 2005
    ..These results indicate that the LAMP assay is an extremely rapid, highly sensitive, specific method. This method is very useful for the rapid detection of periodontopathic bacteria and the diagnosis of periodontal disease...
  5. doi request reprint Hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria
    Akihiro Yoshida
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    J Periodontol 80:1845-51. 2009
    ..Hydrogen sulfide is one of the predominant volatile sulfur compounds (VSCs) produced by oral bacteria. This study developed and evaluated a system for detecting hydrogen sulfide production by oral bacteria...
  6. ncbi request reprint Quantitative detection of volatile sulfur compound- producing microorganisms in oral specimens using real-time PCR
    H Kato
    Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu, Japan
    Oral Dis 11:67-71. 2005
    ..In this study, we report a real-time polymerase chain reaction (PCR) method for the quantitative analysis of VSC-producing bacteria in oral specimens...
  7. doi request reprint Oral health in patients on haemodialysis for diabetic nephropathy and chronic glomerulonephritis
    Gou Teratani
    Division of Community Oral Health Science, Department of Health Promotion, School of Dentistry, Kyushu Dental College, 2 6 1 Manazuru, Kokurakita ku, Kitakyushu 803 8580, Japan
    Clin Oral Investig 17:483-9. 2013
    ..The objective of this study was to clarify differences in oral health status between patients who needed haemodialysis (HD) owing to diabetic nephropathy (DN) and chronic glomerulonephritis (CGN)...
  8. doi request reprint Does decreased diastolic blood pressure associate with increased mortality in 80-year-old Japanese?
    Shuntaro Kagiyama
    Division of General Internal Medicine, Department of Health Promotion, Science of Health Improvement, Kyushu Dental College, Kitakyushu, Japan
    Clin Exp Hypertens 31:639-47. 2009
    ..These results suggest that decreased DBP is associated with higher mortality in the Japanese elderly...
  9. doi request reprint Association of cortisol and dehydroepiandrosterone sulphate levels in serum with periodontal status in older Japanese adults
    Aiko Ishisaka
    Department of Health Promotion, Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    J Clin Periodontol 35:853-61. 2008
    ..In this study, we examined the association between levels of the stress-related steroid hormones cortisol and dehydroepiandrosterone-sulphate (DHEAS) and periodontitis in elderly subjects...
  10. ncbi request reprint Serum albumin levels as an independent predictor of 4-year mortality in a community-dwelling 80-year-old population
    Yutaka Takata
    Division of General Internal Medicine, Kyushu Dental College, Kitakyushu, 803 8580, Japan
    Aging Clin Exp Res 22:31-5. 2010
    ..We, therefore, examined the association in a Japanese population of 80-year-old community residents...
  11. pmc High-level activities of daily living and disease-specific mortality during a 12-year follow-up of an octogenarian population
    Yutaka Takata
    Division of General Internal Medicine, School of Oral Health Science, Kyushu Dental University, Kitakyushu, Japan
    Clin Interv Aging 8:721-8. 2013
    ..We examined whether mortality is associated with high-level activities of daily living in an octogenarian population...
  12. doi request reprint IgA level is associated with risk for mortality in an eighty-year-old population
    Takehiro Torisu
    Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, Kokurakita ku, Kitakyushu, Japan
    Gerontology 55:179-85. 2009
    ..Immunoglobulin levels are elevated in the older people. However, it is unknown whether these levels are related to mortality...
  13. ncbi request reprint Site-specific development of periodontal disease is associated with increased levels of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque
    Tetsuro Mineoka
    Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    J Periodontol 79:670-6. 2008
    ..gingivalis, T. denticola, and T. forsythia in subgingival biofilms and the relationship between the quantity and prevalence of these three bacteria and site-specific periodontal health...
  14. doi request reprint Atrophic gastritis, but not antibody to Helicobacter pylori, is associated with body mass index in a Japanese population
    Takehiro Torisu
    Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, 2 6 1 Manazuru, Kokurakita, Kitakyushu 803 8580, Japan
    J Gastroenterol 43:762-6. 2008
    ..In contrast, epidemiological case-control studies have failed to show an association between HP infection and BMI. Therefore, we investigated whether HP and atrophic gastritis (AG) were associated with BMI...
  15. doi request reprint Salivary levels of cortisol and chromogranin A in patients with dry mouth compared with age-matched controls
    Chieko Shigeyama
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106:833-9. 2008
    ..To evaluate the salivary levels of cortisol and chromogranin A (CgA) in patients with dry mouth (perceived xerostomia and hyposalivation) compared with age-matched controls...
  16. doi request reprint Activities of daily living dependency and disease-specific mortality during 12-year follow-up in an 80-year-old population
    Yutaka Takata
    Division of General Internal Medicine, Kyushu Dental College, Manazuru 2 6 1, Kokurakita ku, Kitakyushu, 803 8580, Japan
    Aging Clin Exp Res 25:193-201. 2013
    ..Therefore, we examined whether 12-year disease-specific mortality may be associated with ADL dependency in an 80-year-old population...
  17. ncbi request reprint Prevalence of periodontopathic bacteria on the tongue dorsum of elderly people
    Masayo Tachibana
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    Gerodontology 23:123-6. 2006
    ..In addition, the relationship between the number of teeth and the microflora present on the coating of the tongue in a population of 85-year-old people was assessed...
  18. ncbi request reprint Possible association of atrophic gastritis and arterial stiffness in healthy middle-aged Japanese
    Takehiro Torisu
    Division of General Internal Medicine, Kyushu Dental College, Japan
    J Atheroscler Thromb 16:691-7. 2009
    ..The aim of the study was to investigate the possible correlation between HP infection and PWV or CAVI in middle-aged subjects...
  19. doi request reprint Body mass index and disease-specific mortality in an 80-year-old population at the 12-year follow-up
    Yutaka Takata
    Division of General Internal Medicine, Kyushu Dental University, Kitakyushu, Japan
    Arch Gerontol Geriatr 57:46-53. 2013
    ..5), and mortality from all-causes or cardiovascular disease was lowest in the group with BMI 22.5 to <23.8...
  20. doi request reprint Correlations between health status and OralChroma™-determined volatile sulfide levels in mouth air of the elderly
    Shuji Awano
    Division of Community Oral Health Science, Department of Health Promotion, School of Dentistry, Kyushu Dental College, 2 6 1 Manazuru, Kokurakita ku, Kitakyushu, Japan
    J Breath Res 5:046007. 2011
    ..The differences, although statistically significant, are quite small. They also indicate that an oral factor, such as a high MM mouth-air level also influences the DMS mouth-air level in addition to systemic factors...
  21. doi request reprint Association of chewing ability with cardiovascular disease mortality in the 80-year-old Japanese population
    Toshihiro Ansai
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    Eur J Cardiovasc Prev Rehabil 15:104-6. 2008
    ..Few have studied the association between chewing ability and longevity...
  22. pmc Relationship between tooth loss and mortality in 80-year-old Japanese community-dwelling subjects
    Toshihiro Ansai
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    BMC Public Health 10:386. 2010
    ..Herein, we determined whether the number of teeth in 80-year-old subjects is an independent predictor of mortality...
  23. pmc Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus
    Akihiro Yoshida
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812 8582, Japan
    J Clin Microbiol 41:4438-41. 2003
    ..The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation...
  24. pmc Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples
    Shiori Nagashima
    Division of Community Oral Health Science, Kyushu Dental College, 2 6 1 Manazuru, Kitakyushu 803 8580, Japan
    J Clin Microbiol 43:2948-51. 2005
    ..The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species...
  25. doi request reprint Relationship between chewing ability and high-level functional capacity in an 80-year-old population in Japan
    Yutaka Takata
    Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, Kitayushu, Japan
    Gerodontology 25:147-54. 2008
    ..To evaluate the association between high-level functional capacity and chewing in a middle-old community-based population...
  26. ncbi request reprint Association between salivary levels of chromogranin A and periodontitis in older Japanese
    Mikiko Hironaka
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    Biomed Res 29:125-30. 2008
    ..In this first known report of the association between CgA level and periodontitis, our results suggest a close relationship between the extent and severity of periodontitis and salivary level of CgA in healthy elderly subjects...
  27. pmc Monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qPCR combined with propidium monoazide
    Ai Yasunaga
    Division of Community Oral Health Development, Kyushu Dental University, 2 6 1 Manazuru, Kokurakita ku Kitakyushu 803 8580, Japan
    BMC Microbiol 13:157. 2013
    ..In the present study, we established an assay for selective analysis of two viable human cariogenic pathogens, S. mutans and S. sobrinus, using PMA combined with real-time PCR (PMA-qPCR)...
  28. ncbi request reprint The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization
    Yoshiaki Masakiyo
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu 803 8580, Japan
    Anaerobe 16:265-9. 2010
    ..Furthermore, this technique will contribute to our understanding of the virulence of these species...
  29. doi request reprint Quality of life and physical fitness in an 85-year-old population
    Yutaka Takata
    Division of General Internal Medicine, Kyushu Dental College, Manazuru 2 6 1, Kokurakita ku, Kitakyushu City 803 8580, Japan
    Arch Gerontol Geriatr 50:272-6. 2010
    ....
  30. pmc Serum total cholesterol concentration and 10-year mortality in an 85-year-old population
    Yutaka Takata
    Division of General Internal Medicine, School of Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    Clin Interv Aging 9:293-300. 2014
    ..These findings suggest that low TC and low LDL-C may be independent predictors of high mortality in the very elderly. ..
  31. doi request reprint Chronic kidney disease increases cardiovascular mortality in 80-year-old subjects in Japan
    Shuntaro Kagiyama
    Division of General Internal Medicine, Department of Health Promotion, Science of Health Improvement, Kyushu Dental College, 2 6 1 Manazuru, Kokurakita ku, Kitakyushu 803 8580, Japan
    Hypertens Res 31:2053-8. 2008
    ..Hypertens Res 2008; 31: 2053-2058)...
  32. doi request reprint Physical fitness and 6.5-year mortality in an 85-year-old community-dwelling population
    Yutaka Takata
    Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, Manazuru 2 6 1, Kokurakita ku, Kitakyushu City 803 8580, Japan
    Arch Gerontol Geriatr 54:28-33. 2012
    ..In conclusion, not only poor muscle strength in handgrip or leg extension, but also nonparticipation in walking test or leg-stepping test were independent predictors of total mortality in a very elderly population...
  33. pmc Associations among hair loss, oral sulfur-containing gases, and gastrointestinal and metabolic linked diseases in Japanese elderly men: pilot study
    Toshihiro Ansai
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    BMC Public Health 9:82. 2009
    ..However, there are no known reports regarding the associations among MPB, and oral sulfur-containing gases, and systemic health conditions in males...
  34. ncbi request reprint Relationship between volatile sulfur compounds in mouth air and systemic disease
    Shuji Awano
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, 2 6 1 Manazuru, Kokurakita ku, Kitakyushu 803 8580, Japan
    J Breath Res 2:017012. 2008
    ..05). These results suggest an association between the production of VSCs in mouth air and systemic diseases such as hypertension as well as respiratory, cerebrovascular and liver diseases...
  35. ncbi request reprint Association of salivary levels of cortisol and dehydroepiandrosterone with periodontitis in older Japanese adults
    Aiko Ishisaka
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    J Periodontol 78:1767-73. 2007
    ....
  36. ncbi request reprint Physical fitness and 4-year mortality in an 80-year-old population
    Yutaka Takata
    Division of General Internal Medicine, Kyushu Dental College, Manazuru 2 6 1, Kokurakita ku, Kitakyushu City 803 8580, Japan
    J Gerontol A Biol Sci Med Sci 62:851-8. 2007
    ..Because little is known about the relationship between physical fitness and mortality among very elderly people, we evaluated this association in a Japanese population of 80-year-old community residents...
  37. pmc Determination of cortisol and dehydroepiandrosterone levels in saliva for screening of periodontitis in older Japanese adults
    Toshihiro Ansai
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu 803 8580, Japan
    Int J Dent 2009:280737. 2009
    ..06 ng/mL and 60.24 pg/mL, respectively, for PD, and 2.12 ng/mL and 61.78 pg/mL, respectively, for CAL. Conclusions. Assessment of hormone levels may be a useful screening method for periodontitis, though limited to never-smokers...
  38. ncbi request reprint [Molecular genetic analysis for oral biofilm formation]
    Akihiro Yoshida
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
    Nihon Saikingaku Zasshi 62:263-9. 2007
  39. doi request reprint The prevalence of periodontopathogenic bacteria in saliva is linked to periodontal health status and oral malodour
    Hiroshi Kurata
    Department of Health Promotion, Kyushu Dental College, 2 6 1 Manazuru, Kokurakitaku, Kitakyushu 803 8580, Japan
    J Med Microbiol 57:636-42. 2008
    ..The prevalence of periodontopathogenic bacteria in saliva may reflect periodontal health status and influence VSC levels in mouth air...
  40. pmc Association between tooth loss and orodigestive cancer mortality in an 80-year-old community-dwelling Japanese population: a 12-year prospective study
    Toshihiro Ansai
    Division of Community Oral Health Development, Kyushu Dental University, Kitakyushu, Japan
    BMC Public Health 13:814. 2013
    ..However, the evidence remains contradictory. This study investigated whether tooth loss, which is indicative of poor oral health and a potential source of oral infections, is associated with death from orodigestive cancer...
  41. ncbi request reprint Association between body mass index and mortality in an 80-year-old population
    Yutaka Takata
    Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    J Am Geriatr Soc 55:913-7. 2007
    ..To evaluate the association between body mass index (BMI) and all-cause mortality and cardiovascular disease (CVD) in an 80-year-old population...
  42. doi request reprint Relationship between root caries and alveolar bone loss in the first wet-rice agriculturalists of the Yayoi period in Japan
    Nobuko Otani
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Japan
    Arch Oral Biol 54:192-200. 2009
    ..Therefore, we identify the factors associated with root caries, and examine the relationship between root caries and alveolar bone loss in the people of the Yayoi period...
  43. ncbi request reprint Salivary levels of hyaluronic acid in female patients with dry mouth compared with age-matched controls: a pilot study
    Yasushi Higuchi
    Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu, Japan
    Biomed Res 30:63-8. 2009
    ..Within the limitations of the present study, patients with dry mouth had lower HA levels in saliva, which may serve as a marker of local dryness or oral mucosa lubrication...
  44. pmc Quantitative analysis of multi-species oral biofilms by TaqMan Real-Time PCR
    Nao Suzuki
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812 8582, Japan
    Clin Med Res 3:176-85. 2005
    ..We review the development and use of TaqMan real-time PCR for quantifying oral bacteria, its role in the diagnosis of oral infectious diseases and their microbial etiology...
  45. doi request reprint A study of the efficacy of ultrasonic waves in removing biofilms
    Takeshi Nishikawa
    Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial Surgery, Kyushu Dental College, Kitakyushu, Japan
    Gerodontology 27:199-206. 2010
    ..The removal of adherent biofilms was assessed using ultrasonic waves in a non-contact mode...
  46. ncbi request reprint Relationship between endothelin-1 and interleukin-1beta in inflamed periodontal tissues
    Tetsuya Rikimaru
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan
    Biomed Res 30:349-55. 2009
    ..These findings indicate that ET-1 is involved in the regulation of IL-1beta expression in gingival tissues and suggest that ET-1 signaling to the cells may be a therapeutic target for treating IL-1beta-dependent inflammatory responses...
  47. ncbi request reprint Rapid LED-based fluorescence microscopy distinguishes between live and dead bacteria in oral clinical samples
    Yoshiaki Masakiyo
    Kyushu Dental College, Kitakyushu, Japan
    Biomed Res 31:21-6. 2010
    ..The LED illumination method characterized in this study can be used for the rapid enumeration of living and dead cells. However, to show specificity, this method requires further innovations...
  48. ncbi request reprint Neutral endopeptidase regulates neurogenic inflammatory responses induced by stimulation of human oral keratinocytes with bacterial lipopolysaccharide and nicotine
    Motoki Nakata
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Japan
    Eur J Oral Sci 121:434-42. 2013
    ..In conclusion, the present study demonstrated that NEP down-regulated the levels of SP and IL-1β produced from human oral keratinocytes, although ECE-1 may be partly related to the down-regulation. ..
  49. doi request reprint Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction
    Yusuke Takahashi
    Division of Community Oral Health Science, Kyushu Dental College, Kokurakita ku, Kitakyushu 803 8580, Japan
    Microbiol Immunol 55:889-92. 2011
    ..Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis...
  50. ncbi request reprint Prediction of cognitive function by arterial stiffness in the very elderly
    Masayo Fukuhara
    Division of General Internal Medicine, Department of Health Promotion, Science of Health Improvement, Kyushu Dental College, Fukuoka, Japan
    Circ J 70:756-61. 2006
    ..Cognitive function is impaired in elderly subjects, so the aim of the present study was to determine the role of arterial stiffness on cognitive function...
  51. ncbi request reprint Volatile sulphide compound levels in mouth air with plaque-related periodontal disease are associated with a change in the proportion of periodontopathic bacteria in saliva
    Hiroshi Kurata
    Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, 2 6 1 Manazuru, Kokurakitaku, Kitakyushu 803 8580, Japan
    J Breath Res 2:017006. 2008
    ..The results suggested that the reduction in VSCs in mouth air of subjects with periodontal disease might be associated with the prevalence of periodontopathic bacteria in saliva...
  52. pmc Development of a 5' fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis
    Akihiro Yoshida
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812 8582, Japan
    J Clin Microbiol 41:863-6. 2003
    ..The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy...
  53. ncbi request reprint Predictive value of microvolt T-wave alternans for sudden cardiac death in patients with preserved cardiac function after acute myocardial infarction: results of a collaborative cohort study
    Takanori Ikeda
    Second Department of Internal Medicine, Kyorin University, Tokyo, Japan
    J Am Coll Cardiol 48:2268-74. 2006
    ..We conducted a collaborative cohort study to evaluate the predictive power of microvolt T-wave alternans (TWA) in patients with preserved left ventricular ejection fraction (LVEF) after myocardial infarction (MI)...
  54. pmc Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation
    Nao Suzuki
    Department of Oral Bacteriology, Ohu University School of Dentistry, Tomitamachi, Koriyama, Japan
    J Clin Microbiol 42:3827-30. 2004
    ..This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms...
  55. ncbi request reprint Streptococcus mutans biofilm formation: utilization of a gtfB promoter-green fluorescent protein (PgtfB::gfp) construct to monitor development
    Akihiro Yoshida
    Department of Oral Biology, State University of New York, 3435 Main St, Buffalo NY 14214, USA
    Microbiology 148:3385-94. 2002
    ..mutans; this analysis demonstrated that gtfC was co-transcribed with gtfB. These results indicated that GFP expression can be utilized to examine gene regulation in S. mutans biofilm formation...
  56. pmc Quantitative microbiological study of subgingival plaque by real-time PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp
    Nao Suzuki
    Department of Oral Bacteriology, Ohu University School of Dentistry, Tomitamachi, Koriyama 963 8611, Japan
    J Clin Microbiol 42:2255-7. 2004
    ..With this assay, the prevalence and proportion of these bacteria in clinical specimens were evaluated. Our preliminary results suggest a positive colocalization of T. forsythensis and Fusobacterium spp. in periodontal pockets...
  57. ncbi request reprint Heterogeneous loss of connexin43 protein in nonischemic dilated cardiomyopathy with ventricular tachycardia
    Hidetsuna Kitamura
    Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan
    J Cardiovasc Electrophysiol 13:865-70. 2002
    ..The current study examines whether qualitative changes or altered distribution of gap junctional connexin43 (Cx43) are related to global ventricular function and ventricular arrhythmia in DCM...
  58. pmc Multiple Streptococcus mutans Genes Are Involved in Biofilm Formation
    Akihiro Yoshida
    Department of Oral Biology, State University of New York, Buffalo 14214, USA
    Appl Environ Microbiol 68:6283-91. 2002
    ..These results suggest that competence genes as well as the sgp and dgk genes may play important roles in S. mutans biofilm formation...
  59. ncbi request reprint Microbial responses using denaturing gradient gel electrophoresis to oil and chemical dispersant in enclosed ecosystems
    Akihiro Yoshida
    Ocean Research Institute, The University of Tokyo, 1 15 1 Minamidai, Nakano Ku, Tokyo 164 8639, Japan
    Mar Pollut Bull 52:89-95. 2006
    ..Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups...
  60. ncbi request reprint Variability in cholesteryl ester transfer protein in healthy Japanese hyper-HDL-cholesterolemic subjects
    Akihiro Yoshida
    Department of Clinical Laboratory, Nakatsugawa Municipal Hospital
    Intern Med 41:357-9. 2002
    ..1% of healthy people). We studied healthy Japanese people in this area with hyper-HDL-cholesterolemia, particularly in relation to CETP...
  61. ncbi request reprint Dramatic enhancement of antagonistic activity on vitamin D receptor: a double functionalization of 1alpha-hydroxyvitamin D3 26,23-lactones
    Nozomi Saito
    Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa 199 0195, Japan
    Org Lett 5:4859-62. 2003
    ..Furthermore, introduction of a 2alpha-methyl group into the 24-methylvitamin D(3) lactones dramatically enhanced their anti-vitamin D activity. [reaction: see text]..
  62. ncbi request reprint Analysis of the factors contributing to serum retinol binding protein and transthyretin levels in Japanese adults
    Akihiro Yoshida
    Department of Clinical Laboratory, Nakatsugawa Municipal Hospital, Nakatsugawa, Gifu, Japan
    J Atheroscler Thromb 13:209-15. 2006
    ..We examined various factors possibly related to metabolic syndrome, particularly focusing on nutritional assessment proteins such as retinol binding protein (RBP) and transthyretin (TTR), and remnant lipoproteins...