Hiroyuki Tanaka

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. ncbi Resveratrol inhibits the release of mediators from bone marrow-derived mouse mast cells in vitro
    Liu Baolin
    Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, P R China
    Planta Med 70:305-9. 2004
  2. ncbi Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I
    Hiroyuki Tanaka
    Laboratory of Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, Japan
    FEBS J 272:4475-86. 2005
  3. ncbi Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Planta Med 73:1127-32. 2007
  4. ncbi Immunochemical approach using monoclonal antibody against Δ(9)-tetrahydrocannabinolic acid (THCA) to discern cannabis plants and to investigate new drug candidates
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Curr Drug Discov Technol 8:3-15. 2011
  5. ncbi Identification and differentiation of Panax species using ELISA, RAPD and eastern blotting
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Phytochem Anal 17:46-55. 2006
  6. ncbi [Cooperative research and educational center for breeding and standardization of medicinal plants and drug development between Japan and China]
    Hiroyuki Tanaka
    Graduate School of Pharmaceutical Sciences, Kyushu University, Japan
    Yakugaku Zasshi 129:393-400. 2009
  7. ncbi Construction and expression of specificity-improved single-chain variable fragments against the bioactive naphthoquinone, plumbagin
    Seiichi Sakamoto
    Department of Medicinal Plants Breeding and Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Biol Pharm Bull 32:434-9. 2009
  8. ncbi Decrease in prostaglandin level is a prerequisite for the expression of cannabinoid withdrawal: a quasi abstinence approach
    Kusnandar Anggadiredja
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan
    Brain Res 1066:201-5. 2005
  9. ncbi Development of sensitivity-improved fluorescence-linked immunosorbent assay using a fluorescent single-domain antibody against the bioactive naphthoquinone, plumbagin
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Anal Bioanal Chem 396:2955-63. 2010
  10. ncbi Flavone-catalyzed apoptosis in Scutellaria baicalensis
    Motosuke Hirunuma
    Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Phytochemistry 72:752-60. 2011

Collaborators

Detail Information

Publications91

  1. ncbi Resveratrol inhibits the release of mediators from bone marrow-derived mouse mast cells in vitro
    Liu Baolin
    Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, P R China
    Planta Med 70:305-9. 2004
    ..In conclusion, resveratrol is a potent non-selective inhibitor of mediator release from activated mast cells and deserves further investigation of its biological modulations...
  2. ncbi Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I
    Hiroyuki Tanaka
    Laboratory of Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, Japan
    FEBS J 272:4475-86. 2005
    ....
  3. ncbi Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Planta Med 73:1127-32. 2007
    ....
  4. ncbi Immunochemical approach using monoclonal antibody against Δ(9)-tetrahydrocannabinolic acid (THCA) to discern cannabis plants and to investigate new drug candidates
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Curr Drug Discov Technol 8:3-15. 2011
    ..Our results indicate the utility of the application of ELISA using MAb-4A4 for further experiments involving marijuana and cannabinoids not only in the forensic field but also in the context of drug discovery...
  5. ncbi Identification and differentiation of Panax species using ELISA, RAPD and eastern blotting
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Phytochem Anal 17:46-55. 2006
    ..An important parameter used for differentiating P. notoginseng is the absence of ginsenoside Rc in the extract of P. notoginseng with eastern blotting. The combination of these methods enabled a reliable identification of Panax spp...
  6. ncbi [Cooperative research and educational center for breeding and standardization of medicinal plants and drug development between Japan and China]
    Hiroyuki Tanaka
    Graduate School of Pharmaceutical Sciences, Kyushu University, Japan
    Yakugaku Zasshi 129:393-400. 2009
    ..Another important aim of the project is to foster young scientists. In the project, we are encouraging them to continue their studies to produce new findings regarding traditional medicines and drug development...
  7. ncbi Construction and expression of specificity-improved single-chain variable fragments against the bioactive naphthoquinone, plumbagin
    Seiichi Sakamoto
    Department of Medicinal Plants Breeding and Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Biol Pharm Bull 32:434-9. 2009
    ..These notable properties of the recombinant antibody against PL made it possible to develop an enzyme-linked immunosorbent assay (ELISA) for reliable determination of PL...
  8. ncbi Decrease in prostaglandin level is a prerequisite for the expression of cannabinoid withdrawal: a quasi abstinence approach
    Kusnandar Anggadiredja
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan
    Brain Res 1066:201-5. 2005
    ..p., given 15 min prior to SR 141716A, blocked the expression of these signs. These results suggested that the decrease in prostaglandin level is a prerequisite for the expression of cannabinoid withdrawal...
  9. ncbi Development of sensitivity-improved fluorescence-linked immunosorbent assay using a fluorescent single-domain antibody against the bioactive naphthoquinone, plumbagin
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Anal Bioanal Chem 396:2955-63. 2010
    ..2 microg mL(-1)), indicating that a sensitive immunoassay could be developed by using fluobody instead of monoclonal antibody or scFv...
  10. ncbi Flavone-catalyzed apoptosis in Scutellaria baicalensis
    Motosuke Hirunuma
    Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Phytochemistry 72:752-60. 2011
    ..Thus, S. baicalensis possesses a unique apoptosis-inducing system that is linked with metabolism of baicalin...
  11. ncbi Construction, expression, and characterization of a single-chain variable fragment antibody against 2,4-dichlorophenoxyacetic acid in the hemolymph of silkworm larvae
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, 812 8582, Japan
    Appl Biochem Biotechnol 164:715-28. 2011
    ....
  12. ncbi Expression, purification, and characterization of anti-plumbagin single-chain variable fragment antibody in Sf9 insect cell
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Hybridoma (Larchmt) 29:481-8. 2010
    ....
  13. ncbi Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    Plant Cell Rep 31:103-10. 2012
    ..The high correlation between the PL-scFv expression level and PL content of the recombinant plants suggested that the PL biosynthesis pathway had been modulated by the expression of PL-scFv protein in the hairy roots of P. zeylanica...
  14. ncbi A fluorescent single domain antibody against plumbagin expressed in silkworm larvae for fluorescence-linked immunosorbent assay (FLISA)
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi ku, Fukuoka, Japan
    Analyst 136:2056-63. 2011
    ....
  15. ncbi Construction and expression of a single chain Fv fragment against pharmacologically active paeoniflorin in Escherichia coli, and its potential use in an enzyme-linked immunosorbent assay
    Zhaohua Lu
    Department of Medicinal Resources Regulation, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Planta Med 72:151-5. 2006
    ..78 to 25 microg/mL. The results obtained by ELISA employing both the recombinant scFv and the original MAbC31B9 showed a reasonably good agreement with each other...
  16. ncbi Preparation of a single-chain variable fragment and a recombinant antigen-binding fragment against the anti-malarial drugs, artemisinin and artesunate, and their application in an ELISA
    Madan K Paudel
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    Anal Chem 84:2002-8. 2012
    ..The recombinant antibody against AM and AS provides an essential component of an economically attractive immunoassay and will be useful in other immunochemical applications for the analysis and purification of antimalarial drugs...
  17. ncbi Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway
    Futoshi Taura
    Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    FEBS Lett 583:2061-6. 2009
    ..The possibility that OLS could be involved in OLA biosynthesis was discussed based on its catalytic properties and expression profile...
  18. ncbi An enzyme-linked immunosorbent assay for aconitine-type alkaloids using an anti-aconitine monoclonal antibody
    Katsumi Kido
    Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Anal Chim Acta 616:109-14. 2008
    ..5microgmL(-1) of Aco. The concentrations of Aco-type alkaloids in various Aconiti radixes assayed using the new ELISA method showed good agreement with previous reports...
  19. ncbi Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody
    Katsumi Kido
    Department of Phamacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    Talanta 77:346-50. 2008
    ..It allowed determination of BA in complex and mixed materials, such as Kampo medicines...
  20. ncbi Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin
    Seiichi Sakamoto
    Department of Medicinal Plant Breeding, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    Anal Chim Acta 607:100-5. 2008
    ..The range for calibration of PL by ELISA was 0.2-25 microg mL(-1). Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of PL in plant...
  21. ncbi Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts
    Osamu Morinaga
    Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825 7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan
    Fitoterapia 81:284-8. 2010
    ..G-Re was first specifically detected and then quantitatively analyzed using NIH Imaging software. We also confirmed that the most suitable solvent was 50% aqueous methanol for extracting G-Re from American ginseng berry pulp...
  22. ncbi Endocannabinoid system modulates relapse to methamphetamine seeking: possible mediation by the arachidonic acid cascade
    Kusnandar Anggadiredja
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Neuropsychopharmacology 29:1470-8. 2004
    ..Extending the current view on the treatment of drug dependence, these results indicate that endocannabinoid-activating substances as well as cyclooxygenase inhibitors may be promising as antirelapse agents...
  23. ncbi Eastern blotting and immunoaffinity concentration using monoclonal antibody for ginseng saponins in the field of traditional chinese medicines
    Hiroyuki Tanaka
    Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Agric Food Chem 55:3783-7. 2007
    ..japonicus by enzyme-linked immunosorbent assay (ELISA) analysis...
  24. ncbi An on-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines
    Osamu Morinaga
    Innovation Plaza Fukuoka, Japan Science and Technology Agency, 3-8-34 Momochihama, Fukuoka, 814-0001, Japan
    Anal Bioanal Chem 383:668-72. 2005
    ..5 microg of GC was clearly detectable. GC can be analyzed quantitatively between 1.0 and 8.0 microg...
  25. ncbi Development of an assay system for saikosaponin a using anti-saikosaponin a monoclonal antibodies
    Shu-Hang Zhu
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Biol Pharm Bull 27:66-71. 2004
    ..The newly established ELISA has been applied for the quantitative assay of SSa in the Bupleuri radix and the Kampo medicines (TCM) prescribed with Bupleuri radix...
  26. ncbi Efficient silkworm expression of single-chain variable fragment antibody against ginsenoside Re using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its application in enzyme-linked immunosorbent assay for quality control of total ginsenosides
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biochem 148:335-40. 2010
    ..05-10 microg/ml. These results clearly suggested that the silkworm expression system is quite useful for the expression of functional scFv that frequently required time- and cost-consuming re-folding when it expressed in E. coli...
  27. ncbi Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs
    Benyakan Pongkitwitoon
    Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Nat Med 65:24-30. 2011
    ....
  28. ncbi Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin
    Madan Kumar Paudel
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    Anal Chim Acta 701:189-93. 2011
    ....
  29. ncbi Analysis of the synergistic effect of glycyrrhizin and other constituents in licorice extract on lipopolysaccharide-induced nitric oxide production using knock-out extract
    Takuhiro Uto
    Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan
    Biochem Biophys Res Commun 417:473-8. 2012
    ....
  30. ncbi Immunohistochemical localization of D-alanine to beta-cells in rat pancreas
    Akiko Morikawa
    Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 355:872-6. 2007
    ..These results indicate for the first time that D-Ala is localized to insulin producing beta-cells in mammalian pancreas, suggesting that this D-amino acid would be involved in the regulation of the blood glucose level...
  31. ncbi Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies
    Osamu Morinaga
    Department of Pharmacognosy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825 7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan
    Fitoterapia 80:28-31. 2009
    ..Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively...
  32. ncbi Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa
    Chiho Taguchi
    Faculty of Pharmaceutical Sciences, Kyushu University, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:217-20. 2008
    ..55 A resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 54.3, b = 110, c = 130 A. These data will enable us to determine the crystal structure of PKS-1...
  33. ncbi Novel miniature SPR immunosensor equipped with all-in-one multi-microchannel sensor chip for detecting low-molecular-weight analytes
    Sook Jin Kim
    Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Kasuga, Fukuoka, Japan
    Biosens Bioelectron 23:701-7. 2007
    ..008 ppb. The miniature SPR sensor demonstrated here for simultaneous analysis of multiple samples with reusability and good storage ability is an important consideration for the advancement of biosensor technology...
  34. ncbi Isolation of ginsenoside Rb1 from Kalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody
    Hiroyuki Tanaka
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Phytother Res 19:255-8. 2005
    ..The result provided evidence that a combination of two immunoassays using monoclonal antibodies against ginsenosides is a powerful means of surveying new ginsenoside resources...
  35. ncbi A quantitative ELISA using monoclonal antibody to survey paeoniflorin and albiflorin in crude drugs and traditional Chinese herbal medicines
    Zhaohua Lu
    Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Biol Pharm Bull 26:862-6. 2003
    ..The developed competitive ELISA was 100 times more sensitive than the HPLC method. Meanwhile, fifteen Chinese traditional prescriptions were determined by the competitive ELISA...
  36. ncbi Immunoquantitative analysis for berberine and its related compounds using monoclonal antibodies in herbal medicines
    Jun-Sik Kim
    Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Analyst 129:87-91. 2004
    ..The ELISA method described in this study can be available as an analytical tool for quality control and standardization of medicinal plants and its prescriptions containing berberine and its related compounds...
  37. ncbi A chimera of green fluorescent protein with single chain variable fragment antibody against ginsenosides for fluorescence-linked immunosorbent assay
    Seiichi Sakamoto
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Protein Expr Purif 77:124-30. 2011
    ..Since some steps required in ELISA can be avoided in this present FLISA, speedy and sensitive immunoassay also could be performed using fluobody instead of monoclonal antibody and scFv...
  38. ncbi The results of surgery under general anesthesia in patients with lung cancer
    Ryuichi Suemitsu
    Department of Thoracic Surgery, Clinical Research Institute, National Hospital Organization, Kyushu Medical Center, Fukuoka, Japan
    Surg Today 41:60-6. 2011
    ..There are few reports of surgical complications for underweight patients. This study evaluated the complications associated with lung cancer surgery and anesthesia in underweight patients in a comparison with obese ones...
  39. ncbi [Development of rapid and sensitive screening methods for natural products using specific monoclonal antibodies]
    Hiroyuki Tanaka
    Department of Medicinal Plant Breeding, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Fukuoka Igaku Zasshi 95:173-82. 2004
  40. ncbi Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract
    Hiroyuki Tanaka
    Graduate School of Pharmaceutical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 0855, Japan
    Int J Anal Chem 2012:260425. 2012
    ....
  41. ncbi Delta(9)-tetrahydrocannabinol enhances an increase of plasma corticosterone levels induced by forced swim-stress
    Kazunori Sano
    Advanced Materials Institute, Fukuoka University, 8 19 1 Nanakuma, Fukuoka, 814 0180, Japan
    Biol Pharm Bull 32:2065-7. 2009
    ..This evidence suggests that THC, under stressful conditions, enhances the susceptibility of the hypothalamus-pituitary-adrenal-axis to stress via the CB(1) receptor, thereby increasing the risk of depression...
  42. ncbi Continuous flow immunosensor for highly selective and real-time detection of sub-ppb levels of 2-hydroxybiphenyl by using surface plasmon resonance imaging
    K Vengatajalabathy Gobi
    Art, Science and Technology Center for Cooperative Research, Kyushu University, Kasuga shi, Fukuoka 816 8580, Japan
    Biosens Bioelectron 20:350-7. 2004
    ..The SPR sensor for HBP is proved to be applicable in simultaneous detection of HBP and BaP in parallel with another SPR sensor for BaP...
  43. ncbi High-throughput determination of free D-aspartic acid in mammals by enzyme immunoassay using specific monoclonal antibody
    Tomohiro Ohgusu
    Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Fukuoka 812-8582, Japan
    Anal Biochem 357:15-20. 2006
    ..The present method provides a simple and high-throughput determination of D-Asp in mammals, and is a useful tool for clarifying the physiological roles and diagnostic values of this D-amino acid...
  44. ncbi Guidelines on the management and treatment of glucocorticoid-induced osteoporosis of the Japanese Society for Bone and Mineral Research (2004)
    Hajime Nawata
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan
    J Bone Miner Metab 23:105-9. 2005
  45. ncbi Sensitive detection of glycyrrhizin and evaluation of the affinity constants by a surface plasmon resonance-based immunosensor
    Takatoshi Sakai
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
    Anal Sci 20:279-83. 2004
    ..The values of the association constant (KA) from three different analyses of ELISA data and from SPR measurements are discussed in detail. As a whole, the affinity constant (KA) between GC-MAb and GC was on the order of 10(7) M(-1)...
  46. ncbi Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using ELISA
    Kiyoshi Noda
    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Phytother Res 16:581-5. 2002
    ..This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains...
  47. ncbi Prostaglandin E2 attenuates SR141716A-precipitated withdrawal in tetrahydrocannabinol-dependent mice
    Kusnandar Anggadiredja
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Brain Res 966:47-53. 2003
    ..p.) also precipitated these withdrawal signs. The results suggest that the expression of THC withdrawal is due to a decrease in prostaglandin levels through inactivation of the arachidonic acid cascade in the brain...
  48. ncbi Identification and characterization of cannabinoids that induce cell death through mitochondrial permeability transition in Cannabis leaf cells
    Satoshi Morimoto
    Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 282:20739-51. 2007
    ..Based on these results, we conclude that CBCA and THCA have the ability to induce necrotic cell death via mitochondrial dysfunction in the leaf cells of C. sativa...
  49. ncbi Anesthetic management of a patient with aortocaval fistula
    Keiko Morikawa
    Department of Anesthesia and Clinical Research Institute, National Hospital Organization Kyushu Medical Center, Chuo Ku, Fukuoka, 810 8563, Japan
    J Anesth 23:111-4. 2009
    ..TEE is a useful tool for monitoring hemodynamics in such patients...
  50. ncbi Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor
    Tatsuhiro Igawa
    Department of Protein Structure, Function and Design, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, 812 8582, Japan
    J Biochem 153:275-82. 2013
    ....
  51. ncbi Creating a unique environment for selecting reactive enzymes with DNA: 'Sticky' binding of oligocation-grafted polymers to DNA
    Hiroyuki Tanaka
    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi ku, Fukuoka 819 0395, Japan
    Bioorg Med Chem 20:1346-53. 2012
    ..The polynucleotides produced by DNA polymerase or RNA polymerase would be captured by the graft copolymers to impede these enzymatic reactions...
  52. ncbi Melanin biosynthesis inhibitory and antioxidant activities of quercetin-3'-O-beta-D-glucoside isolated from Allium cepa
    Enos Tangke Arung
    Department of Agro Environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812 8581, Japan
    Z Naturforsch C 66:209-14. 2011
    ..04 micromol Trolox equivalents/mmol. 1 was shown to be a promising ingredient that could be useful for treating hyperpigmentation and for protecting against oxidative stress...
  53. ncbi Effect of body mass index on perioperative complications in thoracic surgery
    Ryuichi Suemitsu
    Department of Thoracic Surgery, Clinical Research Institute, National Hospital Organization, Kyushu Medical Center, Fukuoka, Japan
    Asian Cardiovasc Thorac Ann 16:463-7. 2008
    ..Both the underweight and the obese are at increased risk of perioperative complications and need to be carefully observed and managed intraoperatively and postoperatively...
  54. ncbi Production, characterization of a monoclonal antibody against aristolochic acid-II and development of its assay system
    Min Tian
    Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, No 38 Xue yuan Road, Haidian District, Beijing 100083, China
    Am J Chin Med 36:425-36. 2008
    ..This is the first time that an ELISA method was successfully established for the application of anti-AA-II MAb...
  55. ncbi Artemisinin production by shoot regeneration of Artemisia annua L. using thidiazuron
    Wanwimon Lualon
    Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
    Z Naturforsch C 63:96-100. 2008
    ..73 -/+ 0.23) microg/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem explant and makes it possible to develop a clonal propagation of A. annua...
  56. ncbi Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re
    Osamu Morinaga
    Innovation Plaza Fukuoka, Japan Science and Technology Agency, Sawara-ku, Fukuoka
    J Chromatogr B Analyt Technol Biomed Life Sci 830:100-4. 2006
    ..The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 microg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 microg...
  57. ncbi A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1
    Waraporn Putalun
    Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002, Khon Kaen, Thailand
    Anal Bioanal Chem 378:1338-41. 2004
    ..Detection limits are 2 microg mL(-1) for both G-Rb1 and G-Rg1...
  58. ncbi Development of a one-step immunochromatographic strip test for the detection of sennosides A and B
    Waraporn Putalun
    Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
    Phytochem Anal 15:112-6. 2004
    ..Detection limits for the strip test were 125 ng/mL for both sennosides. The assay system is useful as a rapid and simple screening method for the detection of 1 and 2 in plants, drugs and body fluids...
  59. ncbi [Management of glucocorticoid-induced osteoporosis in childhood]
    Hiroyuki Tanaka
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Pediatrics
    Clin Calcium 16:1879-86. 2006
    ..But in pediatrics, we are far from the firm evidences. Large scale randomized controlled study (RCT) is necessary...
  60. ncbi [Secondary osteoporosis. Glucocorticoid induced osteoporosis in childhood : prophylaxis and treatment]
    Hiroyuki Tanaka
    Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Pediatrics
    Clin Calcium 17:1114-9. 2007
    ..Recently bisphosphonates have been widely used in prophylaxis and treatment of the steroid induced osteoporosis in adult with large amount of evidences, which is largely unknown for the pediatric patients...
  61. ncbi Activation of a refolded, berberine-specific, single-chain Fv fragment by addition of free berberine
    Jun-Sik Kim
    Department of Oriental Medicine Resources, College of Health and Welfare, Dong-Shin University, 252 Daeho-dong, Naju, Jeonnam 520-714, Republic of Korea
    Biotechnol Lett 28:999-1006. 2006
    ..The results of direct ELISA showed that the anti-berberine scFv retained specific binding activity to berberine. In competitive ELISA, however, activity was increased depending on the concentration of berberine...
  62. ncbi Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences
    Adel Abdel-Aziz Hagras
    Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, Japan
    Genes Genet Syst 80:147-59. 2005
    ..These EST markers would be useful for detecting chromatin introgressed from these species into the wheat genome...
  63. ncbi Chromatographic resolution of glucosidic compounds, ginsenosides on polyethersulphone membrane, and its application to the quantitative immunoassay for ginseng saponins
    Osamu Morinaga
    Innovation Plaza Fukuoka, Japan Science and Technology Agency, 3-8-34 Momochihama, Fukuoka 814-0001, Japan
    Glycobiology 15:1061-6. 2005
    ..At least 62.5 ng of G-Rb1, G-Rc, and G-Rd were clearly detectable individually. Three ginsenosides can be analyzed quantitatively between 0.125 and 2.0 microg...
  64. ncbi Rapid detection of glycyrrhizin by immunochromatographic assay
    Waraporn Putalun
    Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand
    Phytochem Anal 16:370-4. 2005
    ..The immunochromatographic strip test, which has a detection limit of 250 ng/mL, is useful as a rapid screening method for detecting glycyrrhizin in plants, biological fluids and food samples...
  65. ncbi Characterization and diagnostic use of a recombinant single-chain antibody specific for the gp116 envelop glycoprotein of Yellow head virus
    Sorasak Intorasoot
    Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Sri Ayudhaya Road, Rajadhevi, Bangkok 10400, Thailand
    J Virol Methods 143:186-93. 2007
    ..The scFv antibody therefore might find application in rapid, simple and sensitive diagnostic tests to detect YHV in farmed shrimp...
  66. ncbi Ectopic expression of homeodomain protein CDX2 in intestinal metaplasia and carcinomas of the stomach
    Yun-Qing Bai
    Department of Surgery, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, Tokyo, Japan
    Cancer Lett 176:47-55. 2002
    ..Our results thus suggest that CDX2 and CDX1 may play a role during IM formation and gastric carcinogenesis...
  67. ncbi A novel glucosyltransferase involved in steroid saponin biosynthesis in Solanum aculeatissimum
    Atsuko Kohara
    Plant Science Center, RIKEN, Yokohama, Kanagawa 230-0045, Japan
    Plant Mol Biol 57:225-39. 2005
    ..Gene expression analysis revealed that the accumulation of SaGT4A transcripts showed a unique response to wounding stress indicating the involvement of SaGT4A in plant defense system...
  68. ncbi Functional importance of Ca2+-deficient N-terminal lobe of molluscan troponin C in troponin regulation
    Teppei Doi
    Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan
    Arch Biochem Biophys 436:83-90. 2005
    ..Our findings indicate that the N-terminal lobe of molluscan TnC plays important roles in molluscan troponin regulation, despite its inability to bind Ca(2+)...
  69. ncbi Direct action of 1,25-dihydroxyvitamin D on bone: VDRKO bone shows excessive bone formation in normal mineral condition
    Hiroyuki Tanaka
    Department of Pediatrics, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    J Steroid Biochem Mol Biol 89:343-5. 2004
    ..We show that Vitamin D has key roles in bone metabolism negatively...
  70. ncbi Isolation and characterization of a human sperm antigen gene h-Sp-1
    Ri Ichiro Kanazawa
    Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
    Int J Androl 26:226-35. 2003
    ..These findings suggest that the h-Sp-1 molecule is expressed in sperm and testes and plays a role in fertilization...
  71. ncbi Thromboxane A2 modulates interaction of dendritic cells and T cells and regulates acquired immunity
    Kenji Kabashima
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    Nat Immunol 4:694-701. 2003
    ..Similar immune responses were seen in wild-type mice treated with a TP antagonist during the sensitization period. Thus, TXA2-TP signaling modulates acquired immunity by negatively regulating DC-T cell interactions...
  72. ncbi Role of Th2 responses in the development of allergen-induced airway remodelling in a murine model of allergic asthma
    Masato Komai
    Department of Pharmacology, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan
    Br J Pharmacol 138:912-20. 2003
    ..g. using anti-CD4 mAb, is a therapeutic approach for the treatment of airway remodelling in asthma...
  73. ncbi Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A
    Waraporn Putalun
    Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand
    Analyst 127:1328-32. 2002
    ..Good correlation between ELISA and HPLC methods was obtained when crude extracts of plant samples were analyzed. The optimized ELISA was found to be applicable to the determination of total aculeatisides in various plant samples...
  74. ncbi Lactobacillus Acidophilus strain L-92 regulates the production of Th1 cytokine as well as Th2 cytokines
    Akiko Torii
    Department of Pharmacology, Gifu Pharmaceutical University, Gifu, Japan
    Allergol Int 56:293-301. 2007
    ....
  75. ncbi Drastic Ca2+ sensitization of myofilament associated with a small structural change in troponin I in inherited restrictive cardiomyopathy
    Fumiaki Yumoto
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Biochem Biophys Res Commun 338:1519-26. 2005
    ....
  76. ncbi Regulation of dendritic cell maturation and function by Bruton's tyrosine kinase via IL-10 and Stat3
    Yuko Kawakami
    Divisions of Cell Biology and Molecular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 103:153-8. 2006
    ..These negative regulatory functions of Btk in dendritic cells appear to be mediated mainly through autocrine secretion of IL-10 and subsequent activation of Stat3...
  77. ncbi Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I
    Watoo Phrompittayarat
    Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
    Phytochem Anal 18:411-8. 2007
    ..95-62.5 ng/mL of bacopaside I, with a limit of detection of 0.5 ng/mL. The method was validated and the practical use of the ELISA for analysing saponin glycosides from Brahmi was demonstrated...
  78. ncbi Repeated instillations of Dermatophagoides farinae into the airways can induce Th2-dependent airway hyperresponsiveness, eosinophilia and remodeling in mice: effect of intratracheal treatment of fluticasone propionate
    Keiko Wakahara
    Department of Pharmacology, Gifu Pharmaceutical University, Gifu, Japan
    Eur J Pharmacol 578:87-96. 2008
    ..farinae-induced airway remodeling in mice, and this model would be useful for studying mechanisms involved in the development of allergic asthma...
  79. ncbi Spectroscopic and ITC study of the conformational change upon Ca2+-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle
    Fumiaki Yumoto
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 369:109-14. 2008
    ..The change should be the first event of the transmission of Ca(2+) signal to TnI in Tn ternary complex...
  80. ncbi IL-15 levels in patients with acute hepatic failure
    Chikara Yonekawa
    Department of Integrated Medicine, Division of Emergency and Critical Care Medicine, Akita University School of Medicine, 1 1 1 Hondo, Akita 010 8543, Japan
    Res Commun Mol Pathol Pharmacol 115:5-14. 2004
    ..0118). IL-15 levels in the nonsurvivors were significantly higher than those in the survivors (p = 0.0357). Our results suggest that IL-15 overexpression may cause liver injury in human...
  81. ncbi Crystallization and preliminary X-ray analysis of the Ca2+-bound C-terminal lobe of troponin C in complex with a troponin I-derived peptide fragment from Akazara scallop
    Fumiaki Yumoto
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, Bunkyo ku, Tokyo, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:535-7. 2007
    ..1, b = 42.2, c = 60.0 A. The asymmetric unit was assumed to contain one molecular complex of the Akazara scallop TnC C-lobe and TnI fragment, with a Matthews coefficient of 1.83 A(3) Da(-1) and a solvent content of 33.0%...
  82. ncbi Immunomodulatory effects of CpG oligodeoxynucleotides on house dust mite-induced airway inflammation in mice
    Izumi Hirose
    Department of Pharmacology, Gifu Pharmaceutical University, Gifu, Japan
    Int Arch Allergy Immunol 147:6-16. 2008
    ..In the present study, we evaluated the immunomodulatory effects of CpG ODNs on the development of house dust mite-induced airway inflammation and remodeling in mice...
  83. ncbi Structure-function relationships of molluscan troponin T revealed by limited proteolysis
    Hiroyuki Tanaka
    Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, Japan
    Biochim Biophys Acta 1784:1037-42. 2008
    ..These results indicate that the C-terminal extension of molluscan troponin T plays a role in anchoring the troponin complex to actin-tropomyosin filaments and is essential for regulation...
  84. ncbi Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L
    Waraporn Putalun
    Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
    Biotechnol Lett 29:1143-6. 2007
    ..The increase was dose-dependent. Similar treatment of hairy roots with methyl jasmonate (0.2 mM) or yeast extract (2 mg ml(-1)) increased artemisinin production to 1.5 and 0.9 microg mg(-1) dry wt, respectively...
  85. ncbi An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I
    Watoo Phrompittayarat
    Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
    Anal Chim Acta 584:1-6. 2007
    ..95-62.5 ng mL(-1) of bacopaside I and the limit of detection was 0.1 ng mL(-1). The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated...
  86. ncbi Negative effect of chromosome 1A on dough strength shown by modification of 1D addition in durum wheat (Triticum durum)
    Monika Garg
    Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, 4 101 Minami, Koyama, Tottori, 680 8553, Japan
    Theor Appl Genet 114:1141-50. 2007
    ..tauschii...
  87. ncbi Pharmacokinetic study of ginsenosides Rb1 and Rg1 in rat by ELISA using anti-ginsenosides Rb1 and Rg1 monoclonal antibodies
    Zhi Chao
    School of TCM, Southern Medical University, Guangzhou 510515, China
    Am J Chin Med 34:1069-81. 2006
    ..We report in this article the application of this immunoassay in pharmacokinetic study of G-Rb1...
  88. ncbi One-step immunochromatographic separation and ELISA quantification of glycyrrhizin from traditional Chinese medicines
    Jinsen Xu
    The Key Laboratory of Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, People s Republic of China
    J Chromatogr B Analyt Technol Biomed Life Sci 850:53-8. 2007
    ..4-25.1 microg/mg) which is, in good agreement with the results of the HPLC method. Our report provides a rapid, reliable and sensitive approach for one-step separation and quantification of GA...
  89. ncbi Effects of alpha tocopherol and probucol supplements on allergen-induced airway inflammation and hyperresponsiveness in a mouse model of allergic asthma
    Nami Okamoto
    Department of Pediatrics, Osaka Medical College, Takatsuki City, Osaka Prefecture, Japan
    Int Arch Allergy Immunol 141:172-80. 2006
    ..We investigated the role of antioxidants in airway hyperresponsiveness to acetylcholine using young asthma model mice, which were sensitized and stimulated with ovalbumin...
  90. ncbi Sennosides A and B production by hairy roots of Senna alata (L.) Roxb
    Waraporn Putalun
    Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
    Z Naturforsch C 61:367-71. 2006
    ....
  91. ncbi Marked increase in CC chemokine gene expression in both human and mouse mast cell transcriptomes following Fcepsilon receptor I cross-linking: an interspecies comparison
    Toshiharu Nakajima
    Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan
    Blood 100:3861-8. 2002
    ..Interspecies comparison studies at the whole genome expression level should be useful for the interpretation of experimental data obtained in animal models of human pathobiology...