Takeshi Sekiguchi

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. ncbi request reprint Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B
    T Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 276:7246-57. 2001
  2. ncbi request reprint Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin B/cdc2 in vitro
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 356:668-73. 2007
  3. pmc NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nucleic Acids Res 34:4593-608. 2006
  4. ncbi request reprint Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Exp Cell Res 300:213-22. 2004
  5. doi request reprint Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 411:555-61. 2011
  6. ncbi request reprint A novel human nucleolar protein, Nop132, binds to the G proteins, RRAG A/C/D
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 279:8343-50. 2004
  7. pmc Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae
    Yuko Todaka
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Genetics 170:1515-24. 2005
  8. ncbi request reprint Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p
    Yonggang Wang
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 336:639-45. 2005
  9. ncbi request reprint Gtr1p differentially associates with Gtr2p and Ego1p
    Yonggang Wang
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Gene 437:32-8. 2009
  10. ncbi request reprint A temperature-sensitive mutant of the mammalian RNA helicase, DEAD-BOX X isoform, DBX, defective in the transition from G1 to S phase
    Junko Fukumura
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582
    J Biochem 134:71-82. 2003

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B
    T Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 276:7246-57. 2001
    ..Both Rag C and Rag D changed their subcellular localization, depending on the nucleotide-bound state of Rag A. In a similar way, the disruption of S. cerevisiae GTR1 resulted in a change in the localization of Gtr2p...
  2. ncbi request reprint Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin B/cdc2 in vitro
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 356:668-73. 2007
    ..We propose a novel function of cyclin B/cdc2 kinase in mitosis, which is to cause a loss of DDX3 function to repress cyclin A expression and to decrease ribosome biogenesis and translation during mitosis...
  3. pmc NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nucleic Acids Res 34:4593-608. 2006
    ..We propose that NOP132 functions in the recruitment of pre-rRNA processing proteins, including DDX47, to the region where rRNA is transcribed within the nucleolus...
  4. ncbi request reprint Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Exp Cell Res 300:213-22. 2004
    ..DDX3Y, similar to DDX3X, shuttles between the nucleus and cytoplasm in a crm1-dependent manner...
  5. doi request reprint Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 411:555-61. 2011
    ..These results indicate that ubiquitination in the UBL domain of Dsk2 has in vivo functions in the ubiquitin-proteasome pathway in yeast...
  6. ncbi request reprint A novel human nucleolar protein, Nop132, binds to the G proteins, RRAG A/C/D
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 279:8343-50. 2004
    ..Nop132 also interacts with human Nip7 and is colocalized with RRAG A, RRAG C, and Nip7. RNA interference knockdown of Nop132 inhibited cell growth of HeLa cells...
  7. pmc Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae
    Yuko Todaka
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Genetics 170:1515-24. 2005
    ..Here, we propose that Gtr1p is involved in RNA polymerase I and III assembly by its association with Rpc19p and could be a mediator that links growth regulatory signals with ribosome biogenesis...
  8. ncbi request reprint Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p
    Yonggang Wang
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 336:639-45. 2005
    ..The resulting Gtr1p-Gtr2p complex was comprised of an equal amount of Gtr1p and Gtr2p, which inhibited the Rna1p/Yrb2 dependent RanGAP activity. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p...
  9. ncbi request reprint Gtr1p differentially associates with Gtr2p and Ego1p
    Yonggang Wang
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Gene 437:32-8. 2009
    ..Gtr2p mutants S23N and T44N, but not Q66L, rescued the gtr2 disruptant. Our findings indicate that the formation of heterodimers by Gtr1p differs between Gtr2p and Ego1p...
  10. ncbi request reprint A temperature-sensitive mutant of the mammalian RNA helicase, DEAD-BOX X isoform, DBX, defective in the transition from G1 to S phase
    Junko Fukumura
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582
    J Biochem 134:71-82. 2003
    ..5 degrees C for 17 h. The amount of cyclin A mRNA decreased in ts ET24 cells within 4 h after the temperature shift to 39.5 degrees C, consistent with the fact that the entry into the S-phase was delayed by the temperature shift...
  11. doi request reprint Ran GTPase guanine nucleotide exchange factor RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro
    Yukiko Horiike
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Biol Rep 36:717-23. 2009
    ..We conclude that RCC1 undergoes post-translational phosphorylation...
  12. doi request reprint Genetic evidence that Ras-like GTPases, Gtr1p, and Gtr2p, are involved in epigenetic control of gene expression in Saccharomyces cerevisiae
    Takeshi Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 368:748-54. 2008
    ..We propose that Gtr1p and Gtr2p are involved in epigenetic control of gene expression in the TOR signaling pathway...
  13. ncbi request reprint A hamster temperature-sensitive alanyl-tRNA synthetase mutant causes degradation of cell-cycle related proteins and apoptosis
    Yonggang Wang
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582
    J Biochem 135:7-16. 2004
    ..5 degrees C. However, the defects of these mutants released EMAPII, an inducer of apoptosis at 39.5 degrees C. No release of EMAPII occurred in ts ET12 cells at 39.5 degrees C, consistent with the delay of apoptosis in these cells...
  14. ncbi request reprint Beef extract supplementation increases leg muscle mass and modifies skeletal muscle fiber types in rats
    Hiroyuki Yoshihara
    Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University, Fukuoka 812 8581, Japan
    J Nutr Sci Vitaminol (Tokyo) 52:183-93. 2006
    ..Thus, BE supplement increased muscle mass and slow type fiber in EDL. The effects of BE supplement on muscle characteristics were similar to those of exercise. beef extract, fat metabolism, muscle fiber type, muscle mass, L-carnitine..
  15. doi request reprint The structures of Alcaligenes faecalis D-3-hydroxybutyrate dehydrogenase before and after NAD+ and acetate binding suggest a dynamical reaction mechanism as a member of the SDR family
    Md Mominul Hoque
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226 8501, Japan
    Acta Crystallogr D Biol Crystallogr 64:496-505. 2008
    ..Based on these structural features, a reaction mechanism has been proposed...
  16. ncbi request reprint Structure of the nondiscriminating aspartyl-tRNA synthetase from the crenarchaeon Sulfolobus tokodaii strain 7 reveals the recognition mechanism for two different tRNA anticodons
    Yoshiteru Sato
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    Acta Crystallogr D Biol Crystallogr 63:1042-7. 2007
    ..It is speculated that this type of nondiscriminating AspRS has been introduced into Thermus thermophilus through horizontal gene transfer...
  17. doi request reprint Structures of Arthrobacter globiformis urate oxidase-ligand complexes
    Ella Czarina Magat Juan
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, Kanagawa 226 8501, Japan
    Acta Crystallogr D Biol Crystallogr . 2008
    ..In the other crystal structures, lithium borate, 8-azaxanthin and allantoate are bound to the enzyme in a similar manner as uric acid. Based on these AgUOX structures, the enzymatic reaction mechanism of UOX has been proposed...
  18. pmc The structures of pyruvate oxidase from Aerococcus viridans with cofactors and with a reaction intermediate reveal the flexibility of the active-site tunnel for catalysis
    Ella Czarina Magat Juan
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:900-7. 2007
    ..The structures also provide insight into the routes for electron transfer to FAD and the involvement of active-site residues in the catalysis of pyruvate to its products...