Research Topics
Genomes and Genes | Hideo NishitaniSummaryAffiliation: Kyushu University Country: Japan Publications
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Publications
The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phaseH Nishitani
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
J Biol Chem 276:44905-11. 2001..These results suggest that the presence of active hCdt1 may be crucial for determining when licensing is legitimate in human cells...- Proteolysis of DNA replication licensing factor Cdt1 in S-phase is performed independently of geminin through its N-terminal regionHideo Nishitani
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka, 812 8582, Japan
J Biol Chem 279:30807-16. 2004..Our data show that proteolysis and Geminin binding independently inactivate Cdt1 after the onset of S-phase to prevent re-replication... 
Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysisHideo Nishitani
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi ku, Fukuoka, Japan
EMBO J 25:1126-36. 2006..PCNA is essential for Cul4- but not Skp2-directed degradation during DNA replication and following ultraviolet-irradiation. Our data unravel multiple distinct pathways regulating Cdt1 to block re-replication...- DNA replication licensingH Nishitani
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812 8582, Japan
Front Biosci 9:2115-32. 2004..Eukaryotes have developed complex regulatory mechanisms to ensure that origin licensing is coordinated with these events so that genome integrity is preserved during successive cell divisions... - Control of DNA replication licensing in a cell cycleHideo Nishitani
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812 8582, Japan
Genes Cells 7:523-34. 2002..Geminin, whose degradation at the end of mitosis is essential for a new round of licensing, has been shown to bind Cdt1 and negatively regulate it, providing a new insight into the regulation of DNA replication in higher eukaryotes... - Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombeHitoshi Nishijima
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
Mol Biol Cell 17:2524-36. 2006..We also found that SpRna1 formed a stable complex with Xpo1/Crm1 plus Ran-GTP, in the presence of H3... 
Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activitiesTomoyuki Ohba
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Genes Cells 13:571-82. 2008..Over-expression of Spi1-G18V, a Ran GTPase mutant fixed in the GTP-bound form, was lethal to S. pombe Deltasnf2SR. Together, our results indicate that Snf2SR is involved in the Ran GTPase cycle in vivo...- Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregationAyumi Kusano
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Mol Biol Cell 15:4960-70. 2004..Taken together, SpRna1 was suggested to function for constructing the centromeres, by cooperating with Clr4 and Snf2SR. Loss of SpRna1 activity, therefore, caused chromosome missegregation... - Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombeEiji Hirose
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1, Maidashi, Fukuoka 812 8582, Japan
Genes Cells 11:29-46. 2006..As a result, a defect of RanGEF/Pim1 abolished the orchestration of sequential mitotic events, spindle formation, septation and cytokinesis that are essential to produce two identical daughter cells... - Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transductionMasaya Oki
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
Gene 400:114-21. 2007..Furthermore, SpMog1 was co-precipitated with Nxt2 and Cid13... - Caffeine mimics adenine and 2'-deoxyadenosine, both of which inhibit the guanine-nucleotide exchange activity of RCC1 and the kinase activity of ATRHitoshi Nishijima
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Genes Cells 8:423-35. 2003..CONCLUSION: The effect of caffeine on cell-cycle control mimics the biological effect of adenine and 2'-dA, both of which inhibit ATR. dATP, a final metabolite of adenine and 2'-dA, is suggested to inhibit ATR, resulting in PCC... - Identification of a heat-shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoaMasamichi Doiguchi
Laboratory of Zoology, Graduate School of Agriculture, Kyushu University, Higashiku Hakozaki, Fukuoka, Japan
Mol Reprod Dev 74:223-32. 2007..These data suggested that DjB1, which was constitutively expressed in postmeiotic spermatogenic cells in testis, was integrated into spermatozoa as at least two components, that is, sperm head and tail of rodent spermatozoa... - Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathwayNaoyuki Hayashi
Department of Molecular Pathology, Cancer Research Institute, Kanazawa University, 13 1 Takara machi, Kanazawa, Ishikawa 920 0934, Japan
Biochem Biophys Res Commun 353:330-6. 2007..These results indicated that Rad53 was activated by the Tel1 pathway in mec1 gsp1 cells, suggesting that Gsp1 helps regulate the role switching the ATM family kinases Mec1 and Tel1... - Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatinGeorgia Xouri
Laboratory of General Biology, School of Medicine, University of Patras, Patras, Greece
EMBO J 26:1303-14. 2007..We propose that dynamic Cdt1-chromatin associations and the recruitment of Geminin to chromatin provide spatio-temporal control of the licensing process... - Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activityMotoaki Ohtsubo
Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734 8553, Japan
Proc Natl Acad Sci U S A 105:10396-401. 2008.... - Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit BrahmaVassilis Roukos
Laboratory of General Biology, School of Medicine, University of Patras, 26500 Rio, Patras, Greece
J Biol Chem 282:9346-57. 2007.... - Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: synergistic effect with mutant p53 on tumor growth and chromosomal instability--evidence of E2F-1 transcriptional control overPanagiotis Karakaidos
Department of Histology and Embryology, Molecular Carcinogenesis Group, School of Medicine, Univerity of Athens, Greece
Am J Pathol 165:1351-65. 2004..Cumulatively, the above suggest a synergistic effect between hCdt1-hCdc6 overexpression and mutant-p53 over tumor growth and CIN in non-small-cell lung carcinomas... - Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell linesGeorgia Xouri
Laboratory of General Biology, Medical School, University of Patras, Rio, Patras, Greece
Eur J Biochem 271:3368-78. 2004..Our data establish that Cdt1 and geminin are regulated at cell cycle exit, and suggest that the mechanisms controlling Cdt1 and geminin levels may be altered in cancer cells... - Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin bindingNozomi Sugimoto
Virology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuohku, Tokyo 104 0045
J Biol Chem 279:19691-7. 2004..Taken together, these findings suggest that Cdt1 function is also negatively regulated by the Cdk phosphorylation independent of geminin binding... - Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclinHiroaki Seino
Division of Mutagenesis, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
Mol Cell Biol 23:3497-505. 2003.... - Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeastTatsuro Takahashi
Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan
EMBO J 22:964-74. 2003..Our results suggest that association of multiple ORC molecules with a replication origin is required for efficient MCM loading and origin firing in fission yeast... - RanBPM, a nuclear protein that interacts with and regulates transcriptional activity of androgen receptor and glucocorticoid receptorMira A Rao
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada
J Biol Chem 277:48020-7. 2002..These data demonstrate that RanBPM interacts with steroid receptors to selectively modify their activity... - [Control of DNA replication: how to limit replication in a cell cycle]Hideo Nishitani
Laboratory of Biological Signaling, Graduate School of Life Science, University of Hyogo, Japan
Seikagaku 80:661-6. 2008