Hideo Nishitani

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. ncbi request reprint The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase
    H Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 276:44905-11. 2001
  2. ncbi request reprint Proteolysis of DNA replication licensing factor Cdt1 in S-phase is performed independently of geminin through its N-terminal region
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka, 812 8582, Japan
    J Biol Chem 279:30807-16. 2004
  3. pmc Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi ku, Fukuoka, Japan
    EMBO J 25:1126-36. 2006
  4. ncbi request reprint DNA replication licensing
    H Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812 8582, Japan
    Front Biosci 9:2115-32. 2004
  5. ncbi request reprint Control of DNA replication licensing in a cell cycle
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812 8582, Japan
    Genes Cells 7:523-34. 2002
  6. pmc Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe
    Hitoshi Nishijima
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Mol Biol Cell 17:2524-36. 2006
  7. doi request reprint Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activities
    Tomoyuki Ohba
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Genes Cells 13:571-82. 2008
  8. pmc Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation
    Ayumi Kusano
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Biol Cell 15:4960-70. 2004
  9. ncbi request reprint Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe
    Eiji Hirose
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1, Maidashi, Fukuoka 812 8582, Japan
    Genes Cells 11:29-46. 2006
  10. ncbi request reprint Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction
    Masaya Oki
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Gene 400:114-21. 2007

Collaborators

Detail Information

Publications23

  1. ncbi request reprint The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase
    H Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 276:44905-11. 2001
    ..These results suggest that the presence of active hCdt1 may be crucial for determining when licensing is legitimate in human cells...
  2. ncbi request reprint Proteolysis of DNA replication licensing factor Cdt1 in S-phase is performed independently of geminin through its N-terminal region
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka, 812 8582, Japan
    J Biol Chem 279:30807-16. 2004
    ..Our data show that proteolysis and Geminin binding independently inactivate Cdt1 after the onset of S-phase to prevent re-replication...
  3. pmc Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi ku, Fukuoka, Japan
    EMBO J 25:1126-36. 2006
    ..PCNA is essential for Cul4- but not Skp2-directed degradation during DNA replication and following ultraviolet-irradiation. Our data unravel multiple distinct pathways regulating Cdt1 to block re-replication...
  4. ncbi request reprint DNA replication licensing
    H Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812 8582, Japan
    Front Biosci 9:2115-32. 2004
    ..Eukaryotes have developed complex regulatory mechanisms to ensure that origin licensing is coordinated with these events so that genome integrity is preserved during successive cell divisions...
  5. ncbi request reprint Control of DNA replication licensing in a cell cycle
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812 8582, Japan
    Genes Cells 7:523-34. 2002
    ..Geminin, whose degradation at the end of mitosis is essential for a new round of licensing, has been shown to bind Cdt1 and negatively regulate it, providing a new insight into the regulation of DNA replication in higher eukaryotes...
  6. pmc Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe
    Hitoshi Nishijima
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Mol Biol Cell 17:2524-36. 2006
    ..We also found that SpRna1 formed a stable complex with Xpo1/Crm1 plus Ran-GTP, in the presence of H3...
  7. doi request reprint Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activities
    Tomoyuki Ohba
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Genes Cells 13:571-82. 2008
    ..Over-expression of Spi1-G18V, a Ran GTPase mutant fixed in the GTP-bound form, was lethal to S. pombe Deltasnf2SR. Together, our results indicate that Snf2SR is involved in the Ran GTPase cycle in vivo...
  8. pmc Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation
    Ayumi Kusano
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Biol Cell 15:4960-70. 2004
    ..Taken together, SpRna1 was suggested to function for constructing the centromeres, by cooperating with Clr4 and Snf2SR. Loss of SpRna1 activity, therefore, caused chromosome missegregation...
  9. ncbi request reprint Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe
    Eiji Hirose
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1, Maidashi, Fukuoka 812 8582, Japan
    Genes Cells 11:29-46. 2006
    ..As a result, a defect of RanGEF/Pim1 abolished the orchestration of sequential mitotic events, spindle formation, septation and cytokinesis that are essential to produce two identical daughter cells...
  10. ncbi request reprint Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction
    Masaya Oki
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Gene 400:114-21. 2007
    ..Furthermore, SpMog1 was co-precipitated with Nxt2 and Cid13...
  11. ncbi request reprint Caffeine mimics adenine and 2'-deoxyadenosine, both of which inhibit the guanine-nucleotide exchange activity of RCC1 and the kinase activity of ATR
    Hitoshi Nishijima
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Genes Cells 8:423-35. 2003
    ..Both caffeine and the inactivation of RCC1, the guanine-nucleotide exchange factor (GEF) of Ran, induce premature chromatin condensation (PCC) in hamster BHK21 cells arrested in the S-phase, suggesting that RCC1 is a target for caffeine...
  12. ncbi request reprint Identification of a heat-shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoa
    Masamichi Doiguchi
    Laboratory of Zoology, Graduate School of Agriculture, Kyushu University, Higashiku Hakozaki, Fukuoka, Japan
    Mol Reprod Dev 74:223-32. 2007
    ..These data suggested that DjB1, which was constitutively expressed in postmeiotic spermatogenic cells in testis, was integrated into spermatozoa as at least two components, that is, sperm head and tail of rodent spermatozoa...
  13. ncbi request reprint Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway
    Naoyuki Hayashi
    Department of Molecular Pathology, Cancer Research Institute, Kanazawa University, 13 1 Takara machi, Kanazawa, Ishikawa 920 0934, Japan
    Biochem Biophys Res Commun 353:330-6. 2007
    ..These results indicated that Rad53 was activated by the Tel1 pathway in mec1 gsp1 cells, suggesting that Gsp1 helps regulate the role switching the ATM family kinases Mec1 and Tel1...
  14. pmc Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin
    Georgia Xouri
    Laboratory of General Biology, School of Medicine, University of Patras, Patras, Greece
    EMBO J 26:1303-14. 2007
    ..We propose that dynamic Cdt1-chromatin associations and the recruitment of Geminin to chromatin provide spatio-temporal control of the licensing process...
  15. pmc Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity
    Motoaki Ohtsubo
    Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734 8553, Japan
    Proc Natl Acad Sci U S A 105:10396-401. 2008
    ....
  16. ncbi request reprint Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma
    Vassilis Roukos
    Laboratory of General Biology, School of Medicine, University of Patras, 26500 Rio, Patras, Greece
    J Biol Chem 282:9346-57. 2007
    ....
  17. pmc Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: synergistic effect with mutant p53 on tumor growth and chromosomal instability--evidence of E2F-1 transcriptional control over
    Panagiotis Karakaidos
    Department of Histology and Embryology, Molecular Carcinogenesis Group, School of Medicine, Univerity of Athens, Greece
    Am J Pathol 165:1351-65. 2004
    ..Cumulatively, the above suggest a synergistic effect between hCdt1-hCdc6 overexpression and mutant-p53 over tumor growth and CIN in non-small-cell lung carcinomas...
  18. ncbi request reprint Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines
    Georgia Xouri
    Laboratory of General Biology, Medical School, University of Patras, Rio, Patras, Greece
    Eur J Biochem 271:3368-78. 2004
    ..Our data establish that Cdt1 and geminin are regulated at cell cycle exit, and suggest that the mechanisms controlling Cdt1 and geminin levels may be altered in cancer cells...
  19. ncbi request reprint Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding
    Nozomi Sugimoto
    Virology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuohku, Tokyo 104 0045
    J Biol Chem 279:19691-7. 2004
    ..Taken together, these findings suggest that Cdt1 function is also negatively regulated by the Cdk phosphorylation independent of geminin binding...
  20. pmc Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin
    Hiroaki Seino
    Division of Mutagenesis, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 23:3497-505. 2003
    ....
  21. pmc Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast
    Tatsuro Takahashi
    Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    EMBO J 22:964-74. 2003
    ..Our results suggest that association of multiple ORC molecules with a replication origin is required for efficient MCM loading and origin firing in fission yeast...
  22. ncbi request reprint RanBPM, a nuclear protein that interacts with and regulates transcriptional activity of androgen receptor and glucocorticoid receptor
    Mira A Rao
    Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada
    J Biol Chem 277:48020-7. 2002
    ..These data demonstrate that RanBPM interacts with steroid receptors to selectively modify their activity...
  23. ncbi request reprint [Control of DNA replication: how to limit replication in a cell cycle]
    Hideo Nishitani
    Laboratory of Biological Signaling, Graduate School of Life Science, University of Hyogo, Japan
    Seikagaku 80:661-6. 2008