J Nikawa

Summary

Affiliation: Kyushu Institute of Technology
Country: Japan

Publications

  1. ncbi Structural and functional conservation of human and yeast HCP1 genes which can suppress the growth defect of the Saccharomyces cerevisiae ire15 mutant
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Gene 171:107-11. 1996
  2. pmc PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820, Japan
    Nucleic Acids Res 26:860-1. 1998
  3. ncbi Suppression of the Saccharomyces cerevisiae hac1/ire15 mutation by yeast genes and human cDNAs
    J Nikawa
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan
    Gene 201:5-10. 1997
  4. pmc Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Nucleic Acids Res 24:4222-6. 1996
  5. pmc The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology
    M Sugiyama
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820 8502, Japan
    J Bacteriol 183:4985-93. 2001
  6. ncbi Differential regulation of two myo-inositol transporter genes of Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Mol Microbiol 10:955-61. 1993
  7. ncbi A cDNA encoding the human transforming growth factor beta receptor suppresses the growth defect of a yeast mutant
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Gene 149:367-72. 1994
  8. ncbi Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka
    J Biochem 118:39-45. 1995
  9. ncbi Cloning of a human cDNA for CTP-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant
    A Nakashima
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820, Japan
    J Biol Chem 272:9567-72. 1997
  10. ncbi Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Mol Microbiol 16:301-8. 1995

Collaborators

Detail Information

Publications26

  1. ncbi Structural and functional conservation of human and yeast HCP1 genes which can suppress the growth defect of the Saccharomyces cerevisiae ire15 mutant
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Gene 171:107-11. 1996
    ..These results clearly indicate that the products of human and yeast HCP1 are structural and functional homologs, and are involved in expression of genes such as of IN01...
  2. pmc PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820, Japan
    Nucleic Acids Res 26:860-1. 1998
    ..The final step is PCR amplification of the fragment composed of a selectable marker and two flanking sequences with the outermost primers. This method is rapid and needs only short oligonucleotides as primers...
  3. ncbi Suppression of the Saccharomyces cerevisiae hac1/ire15 mutation by yeast genes and human cDNAs
    J Nikawa
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan
    Gene 201:5-10. 1997
    ..All human cDNAs and yeast multicopy suppressors, which had been isolated as suppressors for the ire15 mutation, were able to suppress the inositol-auxotrophic phenotype but not the defect in KAR2 induction of the hac1-disrupted strain...
  4. pmc Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Nucleic Acids Res 24:4222-6. 1996
    ..These results clearly indicate that the IRE2/HAC1 gene product plays a critical role in the induction of KAR2 expression and in the inositol prototrophy mediated by IRE1...
  5. pmc The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology
    M Sugiyama
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820 8502, Japan
    J Bacteriol 183:4985-93. 2001
    ..It is conceivable that the repressor function of this complex is regulated by the C-terminal region of Itc1p...
  6. ncbi Differential regulation of two myo-inositol transporter genes of Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Mol Microbiol 10:955-61. 1993
    ....
  7. ncbi A cDNA encoding the human transforming growth factor beta receptor suppresses the growth defect of a yeast mutant
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Gene 149:367-72. 1994
    ..These results clearly indicate that these human genes function in yeast cells. It is also suggested that yeast possesses a signal transduction mechanism resembling the human TGF-beta-mediated signaling pathway...
  8. ncbi Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka
    J Biochem 118:39-45. 1995
    ..These results clearly indicate that the ire15 and CSE1 mutations genetically interact and the SCS2 and INO2/SCS1 genes are involved in the regulation of INO1 expression...
  9. ncbi Cloning of a human cDNA for CTP-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant
    A Nakashima
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka 820, Japan
    J Biol Chem 272:9567-72. 1997
    ..The cDNA gene product was expressed as a fusion with glutathione S-transferase in Escherichia coli and shown to have ET activity. These results clearly indicate that the cDNA obtained here encodes human ET...
  10. ncbi Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Mol Microbiol 16:301-8. 1995
    ..These results strongly suggest that the DIE1 and DIE2 gene products have an important regulatory function for gene expression of not only ITR1 but also INO1...
  11. ncbi Isolation and characterization of a SCT1 gene which can suppress a choline-transport mutant of Saccharomyces cerevisiae
    M Matsushita
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka
    J Biochem 117:447-51. 1995
    ..SCT1 did not suppress a null mutant of ctr1, indicating that a mutant form of choline transporter is necessary for the suppression caused by SCT1...
  12. pmc The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism
    S Kagiwada
    Animal and Cellular Systems Laboratory, The Institute of Physical and Chemical Research RIKEN, Wako, Saitama, Japan
    J Bacteriol 180:1700-8. 1998
    ..Therefore, the Scs2p function might be conserved among eukaryotic cells...
  13. ncbi Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis
    K Hosaka
    Department of Biochemistry, Gunma University School of Medicine
    J Biochem 115:131-6. 1994
    ..These results confirm and strengthen the conclusion that the SCS1/INO2 gene is required for expression of inositol-choline-regulated genes in phospholipid synthesis...
  14. ncbi Molecular cloning of rat phosphatidylinositol synthase cDNA by functional complementation of the yeast Saccharomyces cerevisiae pis mutation
    S Tanaka
    Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan
    FEBS Lett 393:89-92. 1996
    ..The predicted protein sequence is highly homologous to the previously determined yeast phosphatidylinositol synthase sequence. The cDNA hybridized to a 1.7-kb mRNA that was abundantly expressed in rat brain and kidney...
  15. ncbi Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes
    Y Abe
    First Department of Pathology and Hygiene, Ehime University School of Medicine, Shigenobu, Ehime 791 0295, Japan
    J Biol Chem 276:44003-11. 2001
    ..PRPK was shown to bind to p53 and to phosphorylate p53 at Ser-15. These results indicate that PRPK may play an important role in the cell cycle and cell apoptosis through phosphorylation of p53...
  16. ncbi IRE1 encodes a putative protein kinase containing a membrane-spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan
    Mol Microbiol 6:1441-6. 1992
    ..Disruption of the IRE1 locus caused myo-inositol auxotrophy. The IRE1 product is very likely a protein kinase required for myo-inositol synthesis...
  17. ncbi Isolation and characterization of two distinct myo-inositol transporter genes of Saccharomyces cerevisiae
    J Nikawa
    Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan
    J Biol Chem 266:11184-91. 1991
    ..Northern blot analysis showed that ITR1 mRNA was much more abundant than ITR2 mRNA. The previously isolated myo-inositol transport mutant was determined to be defective in ITR1...
  18. pmc Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae
    P Sass
    Proc Natl Acad Sci U S A 83:9303-7. 1986
    ..These results therefore imply that the effects of RAS2Val19 are mediated through its changes in cAMP concentration...
  19. ncbi Exploring the function of RAS oncogenes by studying the yeast Saccharomyces cerevisiae
    T Toda
    Cold Spring Harbor Laboratory, New York 11724
    Princess Takamatsu Symp 17:253-60. 1986
    ..RAS proteins are themselves probably controlled by growth regulatory proteins...
  20. ncbi Cloning and sequence of the SCS3 gene which is required for inositol prototrophy in Saccharomyces cerevisiae
    K Hosaka
    Department of Biochemistry, Gunma University School of Medicine
    J Biochem 116:1317-21. 1994
    ..Disruption of the SCS3 locus caused myo-inositol auxotrophy. The gene appeared to be involved in the synthesis of inositol phospholipids from inositol but not in the control of inositol synthesis...
  21. ncbi Primary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae
    J Nikawa
    J Biol Chem 262:4876-81. 1987
    ..The nonviable spores were in an arrested state with a characteristic terminal phenotype, suggesting that the function of the PIS gene is essential for progression of the yeast cell cycle...
  22. pmc Cloning of a gene encoding choline transport in Saccharomyces cerevisiae
    J Nikawa
    J Bacteriol 166:328-30. 1986
    ..The cloned plasmids contained a common 4.0-kilobase DNA fragment and also complemented an ethanolamine transport defect. The cloned sequence present in the yeast genome was possibly unique...
  23. ncbi Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae
    Y Tsukagoshi
    Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan
    Eur J Biochem 169:477-86. 1987
    ..coli) and DNA ligase (T4 phage), suggesting that these five enzymes are evolutionarily related. Statistically significant sequence homology was also noted between the human c-fos gene product and the enzyme...
  24. pmc Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae
    J Nikawa
    Cold Spring Harbor Laboratory, New York 11724
    Mol Cell Biol 7:3629-36. 1987
    ..cerevisiae. Disruption of both PDE genes results in a phenotype which resembles that induced by the RAS2(Val-19) mutation. pde1- pde2- ras1- ras2- cells are viable...
  25. ncbi Molecular cloning of the gene encoding CDPdiacylglycerol-inositol 3-phosphatidyl transferase in Saccharomyces cerevisiae
    J Nikawa
    Eur J Biochem 143:251-6. 1984
    ..Thus, the availability of myo-inositol is a critical regulatory factor in yeast phosphatidylinositol synthesis...
  26. ncbi Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation
    K Hosaka
    Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan
    FEBS Lett 304:229-32. 1992
    ..The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not much in the amino-terminal region...