Keiichi I Nakayama

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. pmc Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis
    Ichiro Onoyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka 812 8582, Japan
    J Exp Med 204:2875-88. 2007
  2. pmc An animal model manifesting neurodegeneration and obesity
    Etsuo Susaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Aging (Albany NY) 2:453-6. 2010
  3. ncbi Cip/Kip cyclin-dependent kinase inhibitors: brakes of the cell cycle engine during development
    K Nakayama
    Department of Molecular and Cellular Biology, Kyushu University, Fukuoka, Japan
    Bioessays 20:1020-9. 1998
  4. ncbi Ubiquitin ligases: cell-cycle control and cancer
    Keiichi I Nakayama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
    Nat Rev Cancer 6:369-81. 2006
  5. pmc Impaired germ cell development due to compromised cell cycle progression in Skp2-deficient mice
    Abbas Fotovati
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
    Cell Div 1:4. 2006
  6. ncbi Regulation of the cell cycle at the G1-S transition by proteolysis of cyclin E and p27Kip1
    K I Nakayama
    Department of Molecular and Cellular Biology, Laboratory of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, 812 8582, Japan
    Biochem Biophys Res Commun 282:853-60. 2001
  7. ncbi Skp2-mediated degradation of p27 regulates progression into mitosis
    Keiko Nakayama
    National Institute of Advanced Industrial Science and Technology AIST, Biological Information Research Center, Tokyo 135 0064, Japan
    Dev Cell 6:661-72. 2004
  8. ncbi [Ubiquitin system regulating G1 and S phases of cell cycle]
    Keiichi I Nakayama
    Tanpakushitsu Kakusan Koso 51:1362-9. 2006
  9. pmc Phosphorylation-dependent degradation of c-Myc is mediated by the F-box protein Fbw7
    Masayoshi Yada
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi, Higashi ku, Fukuoka, Fukuoka, Japan
    EMBO J 23:2116-25. 2004
  10. pmc Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development
    Toru Saiga
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Mol Cell Biol 29:3529-43. 2009

Detail Information

Publications76

  1. pmc Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis
    Ichiro Onoyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka 812 8582, Japan
    J Exp Med 204:2875-88. 2007
    ....
  2. pmc An animal model manifesting neurodegeneration and obesity
    Etsuo Susaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Aging (Albany NY) 2:453-6. 2010
    ..Our animal model is thus a powerful tool for studies of the relation between neurodegeneration and obesity...
  3. ncbi Cip/Kip cyclin-dependent kinase inhibitors: brakes of the cell cycle engine during development
    K Nakayama
    Department of Molecular and Cellular Biology, Kyushu University, Fukuoka, Japan
    Bioessays 20:1020-9. 1998
    ..In this review, the biological roles of Cip/Kip CKIs in development and tumor suppression are discussed...
  4. ncbi Ubiquitin ligases: cell-cycle control and cancer
    Keiichi I Nakayama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
    Nat Rev Cancer 6:369-81. 2006
    ..A better understanding of the ubiquitylation machinery will provide new insights into the regulatory biology of cell-cycle transitions and the development of anti-cancer drugs...
  5. pmc Impaired germ cell development due to compromised cell cycle progression in Skp2-deficient mice
    Abbas Fotovati
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
    Cell Div 1:4. 2006
    ..The consequent reduction in the number of mature gametes accounts for the decreased fertility of these animals. These findings reinforce the importance of the Skp2-p27 pathway in cell cycle regulation and in germ cell development...
  6. ncbi Regulation of the cell cycle at the G1-S transition by proteolysis of cyclin E and p27Kip1
    K I Nakayama
    Department of Molecular and Cellular Biology, Laboratory of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, 812 8582, Japan
    Biochem Biophys Res Commun 282:853-60. 2001
    ..In this article, recent progress in characterization of the molecular mechanisms that control the proteolysis of cyclin E and p27Kip1 is reviewed...
  7. ncbi Skp2-mediated degradation of p27 regulates progression into mitosis
    Keiko Nakayama
    National Institute of Advanced Industrial Science and Technology AIST, Biological Information Research Center, Tokyo 135 0064, Japan
    Dev Cell 6:661-72. 2004
    ..These data suggest that p27 proteolysis is necessary for the activation of not only Cdk2 but also Cdc2, and that Skp2 contributes to regulation of G(2)-M progression by mediating the degradation of p27...
  8. ncbi [Ubiquitin system regulating G1 and S phases of cell cycle]
    Keiichi I Nakayama
    Tanpakushitsu Kakusan Koso 51:1362-9. 2006
  9. pmc Phosphorylation-dependent degradation of c-Myc is mediated by the F-box protein Fbw7
    Masayoshi Yada
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi, Higashi ku, Fukuoka, Fukuoka, Japan
    EMBO J 23:2116-25. 2004
    ..Accumulation of c-Myc was also apparent in mouse Fbw7-/- embryonic stem cells. These observations suggest that two F-box proteins, Fbw7 and Skp2, differentially regulate c-Myc stability by targeting MB1 and MB2, respectively...
  10. pmc Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development
    Toru Saiga
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Mol Cell Biol 29:3529-43. 2009
    ..In addition, neuronal migration was impaired in Fbxo45(-)(/)(-) mice. These results suggest that Fbxo45 forms a novel Fbxo45-PAM ubiquitin ligase complex that plays an important role in neural development...
  11. ncbi Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Nat Cell Biol 6:1229-35. 2004
    ..Depletion of KPC1 by RNA interference also inhibited p27(Kip1) degradation. KPC thus probably controls degradation of p27(Kip1) in G1 phase after export of the latter from the nucleus...
  12. pmc Degradation of p57Kip2 mediated by SCFSkp2-dependent ubiquitylation
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 100:10231-6. 2003
    ..These observations thus demonstrate that the SCFSkp2 complex plays an important role in cell-cycle progression by determining the abundance of p57Kip2 and that of the related CDK inhibitor p27Kip1...
  13. ncbi Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis
    Fumihiko Okumura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 279:53533-43. 2004
    ..These findings indicate that E4B serves as a ubiquitin ligase for FEZ1 and thereby regulates its function but not its degradation...
  14. ncbi Fbxw7 contributes to tumor suppression by targeting multiple proteins for ubiquitin-dependent degradation
    Yo Fujii
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582
    Cancer Sci 97:729-36. 2006
    ..Fbxw7 seems to regulate the levels of multiple targets to suppress cancer development...
  15. ncbi U-box protein carboxyl terminus of Hsc70-interacting protein (CHIP) mediates poly-ubiquitylation preferentially on four-repeat Tau and is involved in neurodegeneration of tauopathy
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Fukuoka, Japan
    J Neurochem 91:299-307. 2004
    ..Thus, CHIP is a ubiquitin ligase for four-repeat tau and maintains neuronal survival by regulating the quality control of tau in neurons...
  16. ncbi Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface
    Akiko Mizokami
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, and Station for Collaborative Research, Kyushu University, Fukuoka 812 8582, Japan
    J Neurosci 27:1692-701. 2007
    ..These results suggest that PRIP is implicated in the trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface, probably by acting as a bridging molecule between GABARAP and the receptors...
  17. pmc Fbxw8 is essential for Cul1-Cul7 complex formation and for placental development
    Ryosuke Tsunematsu
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Cell Biol 26:6157-69. 2006
    ..These results suggest that the Cul7-based SCF-like E3 complex has both Fbxw8-dependent and Fbxw8-independent functions...
  18. pmc CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis
    Masaaki Nishiyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Nat Cell Biol 11:172-82. 2009
    ..These observations reveal a mode of p53 regulation mediated by CHD8, which may set a threshold for induction of apoptosis during early embryogenesis by counteracting p53 function through recruitment of histone H1...
  19. pmc VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Genes Dev 18:3055-65. 2004
    ..These data suggest that the functions of the Cul2-Rbx1 and Cul5-Rbx2 modules are distinct...
  20. doi GABA(A) receptor subunit alteration-dependent diazepam insensitivity in the cerebellum of phospholipase C-related inactive protein knockout mice
    Akiko Mizokami
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, and Station for Collaborative Research, Kyushu University, Fukuoka, Japan
    J Neurochem 114:302-10. 2010
    ..PRIP-1 and 2 double KO mice exhibit a diazepam-insensitive phenotype because of a decrease in diazepam-sensitive (alpha1/gamma2) and increase in diazepam-insensitive (alpha6/gamma2) GABA(A) receptors in the cerebellar granule cells...
  21. pmc F-box and WD repeat domain-containing-7 (Fbxw7) protein targets endoplasmic reticulum-anchored osteogenic and chondrogenic transcriptional factors for degradation
    Kanae Yumimoto
    From the Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 288:28488-502. 2013
    ..Collectively, our results suggest that OASIS and BBF2H7 are bona fide substrates of Fbxw7 and that Fbxw7 controls osteogenesis and chondrogenesis by targeting OASIS and BBF2H7, respectively, for degradation. ..
  22. ncbi Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Genes Cells 9:533-48. 2004
    ..These observations suggest that the function of U box-type E3s is to mediate the degradation of unfolded or misfolded proteins in conjunction with molecular chaperones as receptors that recognize such abnormal proteins...
  23. doi Fbxw7β resides in the endoplasmic reticulum membrane and protects cells from oxidative stress
    Akinobu Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Cancer Sci 102:749-55. 2011
    ..Our results thus suggest that Fbxw7β contributes to the protection of cells from oxidative stress...
  24. ncbi Molecular dissection of the interaction between p27 and Kip1 ubiquitylation-promoting complex, the ubiquitin ligase that regulates proteolysis of p27 in G1 phase
    Shuhei Kotoshiba
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582
    J Biol Chem 280:17694-700. 2005
    ..These results suggest that free p27 is recognized by the NH(2)-terminal region of KPC1, which also associates with KPC2, and that p27 is then polyubiquitylated by the COOH-terminal RING-finger domain of KPC1...
  25. doi Loss of FBXW7, a cell cycle regulating gene, in colorectal cancer: clinical significance
    Masaaki Iwatsuki
    Department of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Beppu 874 0838, Japan
    Int J Cancer 126:1828-37. 2010
    ..Genetic alterations in tumors led to the loss of FBXW7 expression and increased cell proliferation. FBXW7 expression provides a prognostic factor for patients with CRC...
  26. pmc Fbxw7-dependent degradation of Notch is required for control of "stemness" and neuronal-glial differentiation in neural stem cells
    Akinobu Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 286:13754-64. 2011
    ..Our results thus implicate Fbxw7 as a key regulator of the maintenance and differentiation of neural stem cells in the brain...
  27. ncbi Anchoring of the 26S proteasome to the organellar membrane by FKBP38
    Tadashi Nakagawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Genes Cells 12:709-19. 2007
    ..These results suggest that FKBP38 functions to anchor the 26S proteasome at the organellar membrane...
  28. pmc Deregulation of the p57-E2F1-p53 axis results in nonobstructive hydrocephalus and cerebellar malformation in mice
    Akinobu Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Fukuoka, Fukuoka 812 8582, Japan
    Mol Cell Biol 31:4176-92. 2011
    ..Our results thus implicate p57 in cell cycle arrest in the subcommissural organ and Pax2-positive interneuron precursors, with the lack of p57 resulting in induction of p53-dependent apoptosis due to hyperactivation of E2F1...
  29. pmc Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis
    Hideo Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi ku, Fukuoka, Japan
    EMBO J 25:1126-36. 2006
    ..PCNA is essential for Cul4- but not Skp2-directed degradation during DNA replication and following ultraviolet-irradiation. Our data unravel multiple distinct pathways regulating Cdt1 to block re-replication...
  30. pmc Phospholipase C-related but catalytically inactive protein is required for insulin-induced cell surface expression of gamma-aminobutyric acid type A receptors
    Makoto Fujii
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, and Station for Collaborative Research, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 285:4837-46. 2010
    ..Taken together, these results suggest that PRIP is involved in insulin-induced GABA(A) receptor insertion by recruiting active Akt to the receptor complex...
  31. pmc Role of the UBL-UBA protein KPC2 in degradation of p27 at G1 phase of the cell cycle
    Taichi Hara
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Cell Biol 25:9292-303. 2005
    ..These observations suggest that KPC2 cooperatively regulates p27 degradation with KPC1 and that the STI1 domain as well as the UBL and UBA domains of KPC2 are indispensable for its function...
  32. doi Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma
    Takehiko Yokobori
    Department of Surgery, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, Beppu 874 0838, Japan
    Int J Oncol 41:253-9. 2012
    ..These genetic alterations of FBXW7 status may provide a prognostic factor for ESCC patients...
  33. pmc Genetic reevaluation of the role of F-box proteins in cyclin D1 degradation
    Tomoharu Kanie
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Mol Cell Biol 32:590-605. 2012
    ..They suggest the existence of other ubiquitin ligases that target cyclin D1 for proteolysis...
  34. pmc Cyclin D2 translocates p27 out of the nucleus and promotes its degradation at the G0-G1 transition
    Etsuo Susaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Cell Biol 27:4626-40. 2007
    ..These results indicate that cyclin D2 translocates p27 from the nucleus into the cytoplasm for its KPC-dependent degradation at the G(0)-G(1) transition...
  35. pmc Fbxw7 regulates lipid metabolism and cell fate decisions in the mouse liver
    Ichiro Onoyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Fukuoka, Japan
    J Clin Invest 121:342-54. 2011
    ..Together, our results suggest that Fbxw7 plays key roles, regulating lipogenesis and cell proliferation and differentiation in the liver...
  36. ncbi Protrudin induces neurite formation by directional membrane trafficking
    Michiko Shirane
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Science 314:818-21. 2006
    ..Thus, protrudin regulates Rab11-dependent membrane recycling to promote the directional membrane trafficking required for neurite formation...
  37. pmc Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex
    Kanae Yumimoto
    From the Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan and CREST, Japan Science and Technology Agency JST, Kawaguchi, Saitama 332 0012, Japan
    J Biol Chem 288:32766-76. 2013
    ..Our results thus provide important insight into the regulation of both SCF ubiquitin ligase activity and circadian rhythmicity. ..
  38. ncbi Cathepsin E prevents tumor growth and metastasis by catalyzing the proteolytic release of soluble TRAIL from tumor cell surface
    Tomoyo Kawakubo
    Department of Pharmacology, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan
    Cancer Res 67:10869-78. 2007
    ..These results thus indicate that cathepsin E plays a substantial role in host defense against tumor cells through TRAIL-dependent apoptosis and/or tumor-associated macrophage-mediated cytotoxicity...
  39. doi Involvement of phospholipase C-related inactive protein in the mouse reproductive system through the regulation of gonadotropin levels
    Miho Matsuda
    Laboratory of Molecular and Cellular Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Biol Reprod 81:681-9. 2009
    ..These results suggest that through gonadotropin secretion, PRIP plays an important role in female reproduction...
  40. ncbi Modulation of GABA(A) receptor phosphorylation and membrane trafficking by phospholipase C-related inactive protein/protein phosphatase 1 and 2A signaling complex underlying brain-derived neurotrophic factor-dependent regulation of GABAergic inhibition
    Takashi Kanematsu
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 281:22180-9. 2006
    ..Together, the results suggest that PRIP plays an important role in BDNF-dependent regulation of GABA(A) receptors by mediating the specific association between beta subunits of these receptors with protein phosphatases...
  41. ncbi Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein
    Hidehisa Takahashi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 280:5611-21. 2005
    ..These results suggest that SUMO-1 functions to tether proteins to PML-containing nuclear bodies through post-translational modification and noncovalent protein-protein interaction...
  42. ncbi Role of serine 10 phosphorylation in p27 stabilization revealed by analysis of p27 knock-in mice harboring a serine 10 mutation
    Yojiro Kotake
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 280:1095-102. 2005
    ..Unexpectedly, the nuclear export of p27 at the G0-G1 transition occurred normally in p27(S10A/S10A) mouse embryonic fibroblasts, indicating that phosphorylation of Ser10 is dispensable for this process...
  43. ncbi Cell cycle-dependent regulation of the Skp2 promoter by GA-binding protein
    Hiroyuki Imaki
    Departments of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Cancer Res 63:4607-13. 2003
    ..These data suggest that the cell cycle-dependent binding of GABP to the Skp2 promoter plays an important role in the regulation of Skp2 expression and cell cycle progression from G(1) to S phase...
  44. pmc Increased E4 activity in mice leads to ubiquitin-containing aggregates and degeneration of hypothalamic neurons resulting in obesity
    Etsuo Susaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 285:15538-47. 2010
    ....
  45. ncbi Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages
    Michiyo Yanagawa
    Department of Pharmacology, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan
    J Biol Chem 282:1851-62. 2007
    ..Our results thus suggest that cathepsin E is important for preventing the accumulation of these lysosomal membrane sialoglycoproteins that can induce a new form of lysosomal storage disorder...
  46. pmc Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation
    Fumiko Matsuzaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Mol Biol Cell 22:4602-20. 2011
    ..Given that mutation of protrudin or KIF5 is a cause of human hereditary spastic paraplegia, the protrudin-KIF5 axis appears to be integral to neuronal function...
  47. pmc Histone H1 recruitment by CHD8 is essential for suppression of the Wnt-β-catenin signaling pathway
    Masaaki Nishiyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Mol Cell Biol 32:501-12. 2012
    ..Given that CHD8 is expressed predominantly during embryogenesis, it may thus contribute to setting a threshold for responsiveness to Wnt signaling that operates in a development-dependent manner...
  48. doi p57 is required for quiescence and maintenance of adult hematopoietic stem cells
    Akinobu Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Cell Stem Cell 9:262-71. 2011
    ..Our results therefore suggest that, among Cip/Kip family CDK inhibitors, p57 plays a predominant role in the quiescence and maintenance of adult HSCs...
  49. pmc Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein
    Shotaro Saita
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 284:13766-77. 2009
    ..These results suggest that VAP-A is an important regulator both of the subcellular localization of protrudin and of its ability to stimulate neurite outgrowth...
  50. pmc Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia
    Yutaka Hashimoto
    From the Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 289:12946-61. 2014
    ..Our results thus suggest that protrudin contributes to the regulation of ER morphology and function, and that its deregulation by mutation is a causative defect in HSP. ..
  51. doi Mice lacking the schizophrenia-associated protein FEZ1 manifest hyperactivity and enhanced responsiveness to psychostimulants
    Nobutaka Sakae
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 2 2 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Hum Mol Genet 17:3191-203. 2008
    ..These observations implicate impairment of FEZ1 function in the pathogenesis of schizophrenia...
  52. doi Selective escape of proteins from the mitochondria during mitophagy
    Shotaro Saita
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Nat Commun 4:1410. 2013
    ..Our results thus show that not all mitochondrial proteins are degraded during mitophagy, with some proteins being evacuated to the ER to prevent unwanted apoptosis...
  53. ncbi Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1
    Kiyotaka Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Japan
    Biochem Biophys Res Commun 303:1209-16. 2003
    ..These observations thus suggest that TIP120A may function as a negative regulator of the SCF complex by binding to nonneddylated Cul1 and thereby preventing assembly of this ubiquitin ligase...
  54. ncbi Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka, Japan
    Proteomics 9:3549-63. 2009
    ..Thus comparative and quantitative studies of tyrosine-phosphorylation have the potential to expand knowledge of signaling networks and to promote understanding of signal transduction at the system level...
  55. pmc Molecular clearance of ataxin-3 is regulated by a mammalian E4
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    EMBO J 23:659-69. 2004
    ..These observations suggest that E4 is a rate-limiting factor in the degradation of pathological forms of ataxin-3, and that targeted expression of E4B is a potential gene therapy for SCA3...
  56. ncbi Regulation of the cell cycle by SCF-type ubiquitin ligases
    Keiichi I Nakayama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Semin Cell Dev Biol 16:323-33. 2005
    ..Clinical evidence also suggests that dysregulation of these F-box proteins contributes to human cancers...
  57. doi Development of mice without Cip/Kip CDK inhibitors
    Yuki Tateishi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Fukuoka, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 427:285-92. 2012
    ....
  58. doi Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin
    Kiyotaka Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Proteome Res 11:796-807. 2012
    ..In conclusion, our method based on K0-Ub is a powerful tool for proteome-wide identification of ubiquitylation sites of target proteins...
  59. ncbi Expression of mouse Fbxw7 isoforms is regulated in a cell cycle- or p53-dependent manner
    Akinobu Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 350:114-9. 2006
    ..Our results thus indicate that expression of Fbxw7 isoforms is differentially regulated in a cell cycle- or p53-dependent manner...
  60. ncbi Cathepsin E-deficient mice show increased susceptibility to bacterial infection associated with the decreased expression of multiple cell surface Toll-like receptors
    Takayuki Tsukuba
    Departments of Pharmacology, Pediatric Dentistry, and Fixed Prosthodontics, Graduate School of Dental Science, Kyushu University, Fukuoka 812 8582
    J Biochem 140:57-66. 2006
    ..These results indicate an essential role of cathepsin E in immune defense against invading microorganisms, most probably due to regulation of the cell surface expression of TLR family members required for innate immune responses...
  61. ncbi Multiple mechanisms for p27(Kip1) translocation and degradation
    Etsuo Susaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Cell Cycle 6:3015-20. 2007
    ..Here we describe our findings and discuss how the multiple potential mechanisms for p27 translocation that precedes its degradation might be integrated in the context of growth stimulation and G(1) progression...
  62. ncbi Large-scale analysis of the human ubiquitin-related proteome
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka, Japan
    Proteomics 5:4145-51. 2005
    ..Our results demonstrate the potential of proteomics analysis of protein ubiquitylation to provide important insight into the regulation of protein stability and other ubiquitin-related cellular functions...
  63. doi Regulation of apoptosis and neurite extension by FKBP38 is required for neural tube formation in the mouse
    Michiko Shirane
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Genes Cells 13:635-51. 2008
    ..Together, our findings suggest that FKBP38 is required for neuroectodermal organization during neural tube formation as a result of its anti-apoptotic activity and regulation of neurite extension...
  64. pmc Mammalian E4 is required for cardiac development and maintenance of the nervous system
    Chie Kaneko-Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Mol Cell Biol 25:10953-64. 2005
    ..UFD2a thus appears to be essential for the development of cardiac muscle, as well as for the protection of spinocerebellar neurons from degeneration induced by endoplasmic reticulum stress...
  65. pmc Early embryonic death in mice lacking the beta-catenin-binding protein Duplin
    Masaaki Nishiyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Mol Cell Biol 24:8386-94. 2004
    ..These results suggest that Duplin plays an indispensable role, likely by a mechanism independent of inhibition of Wnt signaling, in mouse embryonic growth and differentiation at an early developmental stage...
  66. doi Ablation of Fbxw7 eliminates leukemia-initiating cells by preventing quiescence
    Shoichiro Takeishi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Fukuoka, Fukuoka 812 8582, Japan
    Cancer Cell 23:347-61. 2013
    ..Purging of LICs by targeting Fbxw7 to interrupt their quiescence and subsequent treatment with imatinib may thus provide the basis for a promising therapeutic approach to CML...
  67. doi Increased efficiency in the generation of induced pluripotent stem cells by Fbxw7 ablation
    Yasutaka Okita
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, 812 8582, Japan
    Genes Cells 17:768-77. 2012
    ..These results suggest that transient inhibition of Fbxw7 would be a more promising approach to efficient generation of iPSCs than c-Myc over-expression...
  68. ncbi Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular development
    Ryosuke Tsunematsu
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    J Biol Chem 279:9417-23. 2004
    ..Expression of Notch1, -2, or -3 or of cyclin E was unaffected in Fbw7(-/-) embryos. Mammalian Fbw7 thus appears to play an indispensable role in negative regulation of the Notch4-Hey1 pathway and is required for vascular development...
  69. ncbi Fbxw7 in cell cycle exit and stem cell maintenance: insight from gene-targeted mice
    Ichiro Onoyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Cell Cycle 7:3307-13. 2008
    ..Such phenotypes have provided insight into the biological roles of Fbxw7 in cell cycle exit, stem cell maintenance and oncosuppression...
  70. doi Tyrosine kinase 2 plays critical roles in the pathogenic CD4 T cell responses for the development of experimental autoimmune encephalomyelitis
    Akiko Oyamada
    Division of Host Defense, Department of Orthopedic Surgery, Kyushu University, Fukuoka, Japan
    J Immunol 183:7539-46. 2009
    ..Taken together, these results indicate that Tyk2 is critically involved in the pathogenic CD4 T cell responses and thus could be a target molecule for the treatment of autoimmune diseases...
  71. doi Phosphorylation of the chromodomain changes the binding specificity of Cbx2 for methylated histone H3
    Atsushi Hatano
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, Japan
    Biochem Biophys Res Commun 397:93-9. 2010
    ..Phosphorylation of the chromodomain of Cbx2 may therefore serve as a molecular switch that affects the reading of the histone modification code and thereby controls epigenetic cellular memory...
  72. doi Association of cathepsin E deficiency with the increased territorial aggressive response of mice
    Naoki Shigematsu
    Department of Pharmacology, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan
    J Neurochem 105:1394-404. 2008
    ..Our data thus indicate that cathepsin E is associated with the SP/NK-1 receptor signaling system and thereby regulates the aggressive response of the animals to stressors such as territorial challenge...
  73. doi Proteome of ubiquitin/MVB pathway: possible involvement of iron-induced ubiquitylation of transferrin receptor in lysosomal degradation
    Ryo Tachiyama
    Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi ku, Fukuoka 812 8582, Japan
    Genes Cells 16:448-66. 2011
    ..These data suggest that the iron-induced ubiquitylation and degradation of TfR along with MVB pathway physiologically plays an important role in iron homeostasis...
  74. pmc Common and specific roles of the related CDK inhibitors p27 and p57 revealed by a knock-in mouse model
    Etsuo Susaki
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 106:5192-7. 2009
    ..Thus, these observations indicate that p57 and p27 share many characteristics in vivo, but that p57 also performs specific functions not amenable to substitution with p27...
  75. doi p53-Altered FBXW7 expression determines poor prognosis in gastric cancer cases
    Takehiko Yokobori
    Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan
    Cancer Res 69:3788-94. 2009
    ..0033). In conclusion, we showed a role for p53 in the transcriptional regulation of FBXW7 expression in clinical gastric cancer cases and showed that disruption of both p53 and FBXW7 contributes to poor prognosis...
  76. ncbi Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a
    Chie Kaneko
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, 812 8582, Japan
    Biochem Biophys Res Commun 300:297-304. 2003
    ..We also show that UFD2a interacts with VCP (a AAA-family ATPase) that is thought to mediate protein folding. These data implicate UFD2a in the degradation of neuronal proteins by the ubiquitin-proteasome pathway...