Y Nakabeppu

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. ncbi request reprint Molecular genetics and structural biology of human MutT homolog, MTH1
    Y Nakabeppu
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, 812 8582, Fukuoka, Japan
    Mutat Res 477:59-70. 2001
  2. pmc Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria
    T Ohtsubo
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Nucleic Acids Res 28:1355-64. 2000
  3. ncbi request reprint Regulation of intracellular localization of human MTH1, OGG1, and MYH proteins for repair of oxidative DNA damage
    Y Nakabeppu
    Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University and CREST, Japan Science and Technology Corporation, Fukuoka, 812 8582, Japan
    Prog Nucleic Acid Res Mol Biol 68:75-94. 2001
  4. ncbi request reprint MTH1, an oxidized purine nucleoside triphosphatase, protects the dopamine neurons from oxidative damage in nucleic acids caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
    H Yamaguchi
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Cell Death Differ 13:551-63. 2006
  5. ncbi request reprint Functional significance of the conserved residues for the 23-residue module among MTH1 and MutT family proteins
    Y Fujii
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 274:38251-9. 1999
  6. ncbi request reprint Characterization of human polymorphic DNA repair methyltransferase
    R Inoue
    Department of Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan
    Pharmacogenetics 10:59-66. 2000
  7. ncbi request reprint Human Ogg1, a protein involved in the repair of 8-oxoguanine, is inhibited by nitric oxide
    M Jaiswal
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Cancer Res 61:6388-93. 2001
  8. pmc Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen
    D Tsuchimoto
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Nucleic Acids Res 29:2349-60. 2001
  9. pmc Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase
    T Tsuzuki
    Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 98:11456-61. 2001
  10. doi request reprint Galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the dentate gyrus of adult mouse hippocampus
    K Kajitani
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Cell Death Differ 16:417-27. 2009

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Molecular genetics and structural biology of human MutT homolog, MTH1
    Y Nakabeppu
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, 812 8582, Fukuoka, Japan
    Mutat Res 477:59-70. 2001
    ..There is a significant homology between MTH1 protein and the C-terminal half of human MYH protein, which may be involved in the recognition of 8-oxoguanine and 2-hydroxyadenine...
  2. pmc Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria
    T Ohtsubo
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Nucleic Acids Res 28:1355-64. 2000
    ..These hMYH transcripts are likely to encode multiple authentic hMYH polypeptides including the 52, 53 and 57 kDa polypeptides detected in Jurkat cells...
  3. ncbi request reprint Regulation of intracellular localization of human MTH1, OGG1, and MYH proteins for repair of oxidative DNA damage
    Y Nakabeppu
    Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University and CREST, Japan Science and Technology Corporation, Fukuoka, 812 8582, Japan
    Prog Nucleic Acid Res Mol Biol 68:75-94. 2001
    ..We describe the detection of the authentic hOGG1 and hMYH proteins in mitochondria, as well as nuclei in human cells, and how their intracellular localization is regulated by alternative splicing of each transcript...
  4. ncbi request reprint MTH1, an oxidized purine nucleoside triphosphatase, protects the dopamine neurons from oxidative damage in nucleic acids caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
    H Yamaguchi
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Cell Death Differ 13:551-63. 2006
    ..We thus demonstrated that MTH1 protects the dopamine neurons from oxidative damage in the nucleic acids, especially in the mitochondrial DNA of striatal nerve terminals of dopamine neurons...
  5. ncbi request reprint Functional significance of the conserved residues for the 23-residue module among MTH1 and MutT family proteins
    Y Fujii
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 274:38251-9. 1999
    ..Indispensabilities of Val(39), Ile(45), and Leu(53) indicate that an amphipathic property of alpha-helix I consisting of 14 residues of the module (Thr(44)-Gly(58)) is essential to maintain the stable catalytic surface for 8-oxo-dGTPase...
  6. ncbi request reprint Characterization of human polymorphic DNA repair methyltransferase
    R Inoue
    Department of Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan
    Pharmacogenetics 10:59-66. 2000
    ..From these results, we concluded that the wild-type and V1 protein have similar enzymatic and physicochemical properties, while V2 protein is considered to be unstable and rare...
  7. ncbi request reprint Human Ogg1, a protein involved in the repair of 8-oxoguanine, is inhibited by nitric oxide
    M Jaiswal
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Cancer Res 61:6388-93. 2001
    ..These data support the concept that NO-mediated inhibition of DNA contributes to the mutagenic environment of chronic inflammation...
  8. pmc Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen
    D Tsuchimoto
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Nucleic Acids Res 29:2349-60. 2001
    ..Our results suggest that APE2 participates in both nuclear and mitochondrial BER and also that nuclear APE2 functions in the PCNA-dependent BER pathway...
  9. pmc Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase
    T Tsuzuki
    Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 98:11456-61. 2001
    ..The MTH1-deficient mouse will provide a useful model for investigating the role of the MTH1 protein in normal conditions and under oxidative stress...
  10. doi request reprint Galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the dentate gyrus of adult mouse hippocampus
    K Kajitani
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Cell Death Differ 16:417-27. 2009
    ..We therefore concluded that galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the hippocampus...
  11. ncbi request reprint Selective induction of DeltaFosB in the brain after transient forebrain ischemia accompanied by an increased expression of galectin-1, and the implication of DeltaFosB and galectin-1 in neuroprotection and neurogenesis
    H Kurushima
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Cell Death Differ 12:1078-96. 2005
    ..We therefore conclude that DeltaFosB can promote the proliferation of quiescent neuronal precursor cells, thus enhancing neurogenesis after transient forebrain ischemia...
  12. ncbi request reprint Expression of the ada gene of Escherichia coli in response to alkylating agents. Identification of transcriptional regulatory elements
    T Nakamura
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Mol Biol 202:483-94. 1988
    ..Some base substitution mutants showed a constitutive expression of the gene in vivo, but this observation did not reconcile with findings in experiments in vitro...
  13. pmc Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism
    H Oda
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Nucleic Acids Res 27:4335-43. 1999
    ..Amounts of each polypeptide reflected copy number of the GC allele in each cell line. There is an apparent linkage dis-equilibrium between the two polymorphic sites, GT/GC at exon 2c and Val83/Met83 at codon 83 for p18...
  14. ncbi request reprint Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions
    K Takano
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Mol Biol 201:261-71. 1988
    ..The latter finding suggests that the cysteine residue at position 321, which is located near the C terminus of the Ada protein, is involved in regulating activity, as the transcriptional activator...
  15. pmc Expression and differential intracellular localization of two major forms of human 8-oxoguanine DNA glycosylase encoded by alternatively spliced OGG1 mRNAs
    K Nishioka
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Mol Biol Cell 10:1637-52. 1999
    ..Deletion mutant analyses revealed that the unique C terminus of OGG1-2a and its mitochondrial targeting signal are essential for mitochondrial localization and that nuclear localization of OGG1-1a depends on the NLS at its C terminus...
  16. ncbi request reprint Regulation of expression of the human MTH1 gene encoding 8-oxo-dGTPase. Alternative splicing of transcription products
    H Oda
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 82, Japan
    J Biol Chem 272:17843-50. 1997
    ..There is a polymorphic alteration at the 5' splicing site (GT to GC) located in exon 2, an event which affects splicing patterns of the MTH1 transcript. Allele frequency of this polymorphism is about 20% among healthy volunteers...
  17. pmc CpG methylation of MGMT and hMLH1 promoter in hepatocellular carcinoma associated with hepatitis viral infection
    S Matsukura
    Department of Biochemistry, Saga Medical School, Japan
    Br J Cancer 88:521-9. 2003
    ..A correlation between the promoter methylation status and viral infection, although it was weak, intimated that hepatitis viral infections could play a role in the CpG methylation of the MGMT promoter...
  18. ncbi request reprint Requirement for two conserved cysteine residues in the Ada protein of Escherichia coli for transactivation of the ada promoter
    A Taketomi
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Mol Gen Genet 250:523-32. 1996
    ....
  19. pmc Accumulation of 8-oxo-2'-deoxyguanosine and increased expression of hMTH1 protein in brain tumors
    T Iida
    Department of Neuropathology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University 60, Fukuoka, 812-8582, Japan
    Neuro Oncol 3:73-81. 2001
    ..Thus, the defense mechanism against such oxidative stress may be enhanced as well. These results suggest that oxidative stress may play a role in tumor progression...
  20. ncbi request reprint DeltaFosB, but not FosB, induces delayed apoptosis independent of cell proliferation in the Rat1a embryo cell line
    K Tahara
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Maidashi Higashi ku, Fukuoka, Japan
    Cell Death Differ 10:496-507. 2003
    ..The cell death was not suppressed by exogenous His-PTD-Bcl-x(L) at all, suggesting involvement of a Bcl-x(L)-resistant pathway for cytochrome c release...
  21. doi request reprint ITPase-deficient mice show growth retardation and die before weaning
    M Behmanesh
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Cell Death Differ 16:1315-22. 2009
    ....
  22. ncbi request reprint Galectin-1beta, a natural monomeric form of galectin-1 lacking its six amino-terminal residues promotes axonal regeneration but not cell death
    T Miura
    Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Maidashi Higashi ku, Fukuoka, Japan
    Cell Death Differ 11:1076-83. 2004
    ..Our results suggest that the biochemical properties of rmGal-1beta determine its biological functions...
  23. ncbi request reprint Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria
    D Kang
    Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan
    J Biol Chem 270:14659-65. 1995
    ..Thus, the 8-oxo-dGTPase encoded by MTH1 gene is localized in mitochondrial and cytosol...
  24. ncbi request reprint Metabolic fate of oxidized guanine ribonucleotides in mammalian cells
    H Hayakawa
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka 812 8582, Japan
    Biochemistry 38:3610-4. 1999
    ..This step appears to serve as a gatekeeper to prevent formation of mutagenic substrates for DNA synthesis from oxidized ribonucleotides...
  25. ncbi request reprint Molecular cloning of AtMMH, an Arabidopsis thaliana ortholog of the Escherichia coli mutM gene, and analysis of functional domains of its product
    T Ohtsubo
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Mol Gen Genet 259:577-90. 1998
    ..However, the adjacent amino acid sequence (residues 268 to 281) is essential for repair activity...
  26. doi request reprint Genomic and functional analyses of MUTYH in Japanese patients with adenomatous polyposis
    R Yanaru-Fujisawa
    Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Clin Genet 73:545-53. 2008
    ..Homozygous MUTYH mutation accounts for approximately 10% of Japanese patients with adenomatous polyposis. G272E may be one of the mutations specific to patients with adenomatous polyposis in East Asia...
  27. ncbi request reprint Expression of hMTH1 in the hippocampi of control and Alzheimer's disease
    A Furuta
    Department of Neuropathology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    Neuroreport 12:2895-9. 2001
    ..We suggest that the expression of hMTH1 indicates indirect evidence of oxidative stress and its regulation is regionally differentiated in AD...
  28. ncbi request reprint A defect in a single allele of the Mlh1 gene causes dissociation of the killing and tumorigenic actions of an alkylating carcinogen in methyltransferase-deficient mice
    H Kawate
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Carcinogenesis 21:301-5. 2000
    ..Loss of Mlh1 expression was frequently observed in tumors of Mgmt(-/-) Mlh1(+/-) mice and this might be related to progression of the tumors...
  29. ncbi request reprint Genomic structure and chromosome location of the human mutT homologue gene MTH1 encoding 8-oxo-dGTPase for prevention of A:T to C:G transversion
    M Furuichi
    Department of Biochemistry, Kyushu University, Fukuoka, Japan
    Genomics 24:485-90. 1994
    ..We isolated the genomic sequence encoding the enzyme and named the gene MTH1 (for mutT human homologue). This gene is composed of at least 4 exons, spans approximately 9 kb, and is located on human chromosome 7p22...
  30. pmc A variant form of hMTH1, a human homologue of the E coli mutT gene, correlates with somatic mutation in the p53 tumour suppressor gene in gastric cancer patients
    Y Kimura
    J Med Genet 41:e57. 2004
  31. ncbi request reprint Intracellular distribution of the antimutagenic enzyme MTH1 in the liver, kidney and testis of F344 rats and its modulation by cadmium
    K S Kasprzak
    Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, MD 21702 1201, USA
    Exp Toxicol Pathol 53:325-35. 2001
    ....
  32. pmc Human MTH1 protein hydrolyzes the oxidized ribonucleotide, 2-hydroxy-ATP
    K Fujikawa
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Nucleic Acids Res 29:449-54. 2001
    ..We suggest that the 2-hydroxy-ATP hydrolyzing activity of MTH1 might prevent the perturbation of these ATP-related pathways by the oxidized ATP...
  33. ncbi request reprint Presence of potential nickel-responsive element(s) in the mouse MTH1 promoter
    R Liang
    Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick, Maryland 21702, USA
    Ann Clin Lab Sci 31:91-8. 2001
    ..The results suggest that up-regulation of murine MTH1 expression by nickel(II) is controlled by the repeat sequences, potential candidates for nickel-responsive elements...
  34. ncbi request reprint A naturally occurring truncated form of FosB that inhibits Fos/Jun transcriptional activity
    Y Nakabeppu
    Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Cell 64:751-9. 1991
    ..In stimulated cells delta FosB may act to limit the transcriptional effects of Fos and Jun proteins...
  35. ncbi request reprint Isoforms of JSAP1 scaffold protein generated through alternative splicing
    M Ito
    Department of Biosciences, School of Science, Kitasato University, Sagamihara, 228 8555, Kanagawa, Japan
    Gene 255:229-34. 2000
    ..These results suggest that JSAP1c and JSAP1d may attenuate the scaffolding activity of JSAP1a and/or JSAP1b in JNK cascades, especially the JNK3 cascades...
  36. ncbi request reprint Induction of apoptosis and cellular senescence in mice lacking transcription elongation factor, Elongin A
    K Miyata
    Department of Functional Genomics, Kochi Medical School, Kohasu, Oko cho, Nankoku, Kochi, Japan
    Cell Death Differ 14:716-26. 2007
    ..Taken together, our results suggest that Elongin A is required for the transcription of genes essential for early embryonic development and downregulation of its activity is tightly associated with cellular senescence...
  37. ncbi request reprint Cloning and expression of the Bacillus subtilis methyltransferase gene in Escherichia coli ada- cells
    K I Kodama
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Mutat Res 218:153-63. 1989
    ..The structural similarity of these methyltransferases suggests possible evolution from a single ancestral gene...
  38. ncbi request reprint DNA binding activities of three murine Jun proteins: stimulation by Fos
    Y Nakabeppu
    Howard Hughes Medical Institute Laboratory, Baltimore, Maryland
    Cell 55:907-15. 1988
    ..If there are functional differences between them, they are likely to involve other activities of the Jun proteins...
  39. pmc The basic region of Fos mediates specific DNA binding
    Y Nakabeppu
    Howard Hughes Medical Institute, Baltimore, MD
    EMBO J 8:3833-41. 1989
    ..Our results support a model in which the Fos and Jun basic regions of the Fos-Jun heterodimer each interact with symmetrical DNA half sites...
  40. ncbi request reprint Purification and structure of 3-methyladenine-DNA glycosylase I of Escherichia coli
    K Sakumi
    J Biol Chem 261:15761-6. 1986
    ..Only 3-methyladenine was excised from methylated DNA by the purified glycosylase. These results show that the tag is the structural gene for 3-methyladenine-DNA glycosylase I...
  41. ncbi request reprint Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents
    Y Nakabeppu
    J Biol Chem 259:13730-6. 1984
    ..Other DNA-damaging agents, such as ethylmethane sulfonate, nalidixic acid, and ultraviolet light did not induce the enzyme activity. The induction was controlled by the ada and adc, but not by the recA and lexA genes...
  42. ncbi request reprint Cloning and characterization of the alkA gene of Escherichia coli that encodes 3-methyladenine DNA glycosylase II
    Y Nakabeppu
    J Biol Chem 259:13723-9. 1984
    ..Taking advantage of overproduction of the alkA protein in adapted cells that harbor multicopy plasmids carrying the alkA+ gene, 3-methyladenine DNA glycosylase II has been purified to apparent physical homogeneity...
  43. pmc Functional significance of conserved residues in the phosphohydrolase module of Escherichia coli MutT protein
    H Shimokawa
    Department of Biology and Frontier Research Center, Fukuoka Dental College, Fukuoka 814 0193, Japan
    Nucleic Acids Res 28:3240-9. 2000
    ....
  44. ncbi request reprint Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli
    Y Yamagata
    Faculty of Pharmaceutical Sciences, Osaka University, Japan
    Cell 86:311-9. 1996
    ..The structure of the active site implies broad substrate specificity and simple N-glycosylase activity...
  45. ncbi request reprint Expression and prognostic significance of O6-methylguanine-DNA methyltransferase in hepatocellular, gastric, and breast cancers
    S Matsukura
    Department of Surgery, Saga Medical School, Japan
    Ann Surg Oncol 8:807-16. 2001
    ..These findings may help to establish therapeutic strategies for patients with these types of solid cancer...