Research Topics
Genomes and Genes | Tomotake KankiSummaryAffiliation: Kyushu University Country: Japan Publications
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Publications
A genomic screen for yeast mutants defective in mitophagyTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
Autophagy 6:278-80. 2010..Accordingly, we hypothesize that Ylr356w is required to detect or present aged or dysfunctional mitochondria when cells reach the post-log phase...
Nix, a receptor protein for mitophagy in mammalsTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
Autophagy 6:433-5. 2010..Recently, it was reported that Nix is a mitochondrial receptor that can directly connect to one of the autophagic machinery components, the Atg8 homologs LC3 and GABARAP...- Monitoring mitophagy in yeast: the Om45-GFP processing assayTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
Autophagy 5:1186-9. 2009..Following the uptake of mitochondria into the vacuole, Om45-GFP is degraded, releasing the intact form of GFP, which is detected by immunoblotting. Therefore, the amount of free GFP reflects the level of mitophagy... - Atg32 is a tag for mitochondria degradation in yeastTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
Autophagy 5:1201-2. 2009..When mitophagy is induced, Atg32 binds to Atg11, an adaptor protein for selective autophagy. Eventually, this interaction results in the recruitment of mitochondria to the vacuole for degradation... - PDIP38 associates with proteins constituting the mitochondrial DNA nucleoidXiaoli Cheng
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582
J Biochem 138:673-8. 2005..PDIP38 associates with the nucleoid components and could be involved in the metabolism of mitochondrial DNA... - The C-terminal tail of mitochondrial transcription factor a markedly strengthens its general binding to DNAKippei Ohgaki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
J Biochem 141:201-11. 2007..Thus, the C-terminal tail of TFAM is important for the strong general binding to mtDNA. This strong DNA-binding conferred by the C-tail may play an important role in the nucleoid structure... - Mitochondrial nucleoid and transcription factor ATomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan
Ann N Y Acad Sci 1011:61-8. 2004..TFAM is tightly associated with mtDNA as a main component of the nucleoid... - Architectural role of mitochondrial transcription factor A in maintenance of human mitochondrial DNATomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Mol Cell Biol 24:9823-34. 2004..Thus, the mtDNA amount is finely correlated with the amount of TFAM but not with the transcription level. We discuss an architectural role for TFAM in the maintenance of mtDNA in addition to its role in transcription activation... - Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeastYusuke Kurihara
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
J Biol Chem 287:3265-72. 2012..Mitophagy contributes to this process by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production... 
Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteinsShinya Matsumoto
Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Mitochondrion 12:391-8. 2012..The RNA-binding protein Y-box binding protein-1 is localized at the mitochondrial outer membrane and bound to the OXPHOS mRNAs. Our findings offer new insight into mitochondrial co-translational import in human cells...- p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding abilityMikako Yagi
Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Nucleic Acids Res 40:9717-37. 2012..Co-immunoprecipitation revealed the close association of p32 with the mitoribosome. We propose that p32 is required for functional mitoribosome formation to synthesize proteins within mitochondria... - Phosphorylation of Serine 114 on Atg32 mediates mitophagyYoshimasa Aoki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
Mol Biol Cell 22:3206-17. 2011..We also found that Hog1 and Pbs2, which are involved in the osmoregulatory signal transduction cascade, are related to Atg32 phosphorylation and mitophagy... - Protein instability and functional defects caused by mutations of dihydro-orotate dehydrogenase in Miller syndrome patientsJingxian Fang
Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi ku, Fukuoka, Japan
Biosci Rep 32:631-9. 2012.... - Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNAShinya Matsumoto
Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
Biochem J 443:573-84. 2012..Taken together, our findings suggest that YB-1 is a critical factor for translation that may control OXPHOS activity... - A genomic screen for yeast mutants defective in selective mitochondria autophagyTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
Mol Biol Cell 20:4730-8. 2009..Accordingly, we have named this gene ATG33. The new mutants identified in this analysis will provide a useful foundation for researchers interested in the study of mitochondrial homeostasis and quality control... - Mitochondria autophagy in yeastTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
Antioxid Redox Signal 14:1989-2001. 2011..In this article, we review recent advances in the physiology and molecular mechanism of mitophagy and discuss the similarities and differences of this degradation process between yeast and mammalian cells... - The molecular mechanism of mitochondria autophagy in yeastTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
Mol Microbiol 75:795-800. 2010..Here, we review the current knowledge on mitophagy, in particular on the molecular mechanism and regulation of mitophagy in yeast. We also discuss some of the differences between yeast and mammalian mitophagy... - The physiological role of mitophagy: new insights into phosphorylation eventsYuko Hirota
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Int J Cell Biol 2012:354914. 2012..In this paper, we describe the molecular processes and regulatory mechanisms of mitophagy in yeast and mammalian cells... - Human mitochondrial DNA is packaged with TFAMTanfis Istiaq Alam
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Nucleic Acids Res 31:1640-5. 2003..Based on the fact that TFAM is abundant enough to wrap mtDNA entirely, these results suggest that human mtDNA is packaged with TFAM... - The N-terminal region of the transmembrane domain of human erythrocyte band 3. Residues critical for membrane insertion and transport activityTomotake Kanki
Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812 8582, Japan
J Biol Chem 278:5564-73. 2003..Finally, deletion of the cytoplasmic surface sequence G(381)LVRD abolished chloride transport, but not surface expression, indicating that this sequence makes an essential structural contribution to the anion transport site of band 3...