Tomotake Kanki

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. pmc The physiological role of mitophagy: new insights into phosphorylation events
    Yuko Hirota
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Int J Cell Biol 2012:354914. 2012
  2. pmc A genomic screen for yeast mutants defective in mitophagy
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 6:278-80. 2010
  3. ncbi request reprint Nix, a receptor protein for mitophagy in mammals
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 6:433-5. 2010
  4. pmc Monitoring mitophagy in yeast: the Om45-GFP processing assay
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 5:1186-9. 2009
  5. ncbi request reprint Atg32 is a tag for mitochondria degradation in yeast
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 5:1201-2. 2009
  6. ncbi request reprint PDIP38 associates with proteins constituting the mitochondrial DNA nucleoid
    Xiaoli Cheng
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582
    J Biochem 138:673-8. 2005
  7. ncbi request reprint The C-terminal tail of mitochondrial transcription factor a markedly strengthens its general binding to DNA
    Kippei Ohgaki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    J Biochem 141:201-11. 2007
  8. ncbi request reprint Mitochondrial nucleoid and transcription factor A
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Ann N Y Acad Sci 1011:61-8. 2004
  9. pmc Architectural role of mitochondrial transcription factor A in maintenance of human mitochondrial DNA
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Cell Biol 24:9823-34. 2004
  10. pmc Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast
    Yusuke Kurihara
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    J Biol Chem 287:3265-72. 2012

Collaborators

Detail Information

Publications22

  1. pmc The physiological role of mitophagy: new insights into phosphorylation events
    Yuko Hirota
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Int J Cell Biol 2012:354914. 2012
    ..In this paper, we describe the molecular processes and regulatory mechanisms of mitophagy in yeast and mammalian cells...
  2. pmc A genomic screen for yeast mutants defective in mitophagy
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 6:278-80. 2010
    ..Accordingly, we hypothesize that Ylr356w is required to detect or present aged or dysfunctional mitochondria when cells reach the post-log phase...
  3. ncbi request reprint Nix, a receptor protein for mitophagy in mammals
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 6:433-5. 2010
    ..Recently, it was reported that Nix is a mitochondrial receptor that can directly connect to one of the autophagic machinery components, the Atg8 homologs LC3 and GABARAP...
  4. pmc Monitoring mitophagy in yeast: the Om45-GFP processing assay
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 5:1186-9. 2009
    ..Following the uptake of mitochondria into the vacuole, Om45-GFP is degraded, releasing the intact form of GFP, which is detected by immunoblotting. Therefore, the amount of free GFP reflects the level of mitophagy...
  5. ncbi request reprint Atg32 is a tag for mitochondria degradation in yeast
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 5:1201-2. 2009
    ..When mitophagy is induced, Atg32 binds to Atg11, an adaptor protein for selective autophagy. Eventually, this interaction results in the recruitment of mitochondria to the vacuole for degradation...
  6. ncbi request reprint PDIP38 associates with proteins constituting the mitochondrial DNA nucleoid
    Xiaoli Cheng
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582
    J Biochem 138:673-8. 2005
    ..PDIP38 associates with the nucleoid components and could be involved in the metabolism of mitochondrial DNA...
  7. ncbi request reprint The C-terminal tail of mitochondrial transcription factor a markedly strengthens its general binding to DNA
    Kippei Ohgaki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    J Biochem 141:201-11. 2007
    ..Thus, the C-terminal tail of TFAM is important for the strong general binding to mtDNA. This strong DNA-binding conferred by the C-tail may play an important role in the nucleoid structure...
  8. ncbi request reprint Mitochondrial nucleoid and transcription factor A
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Ann N Y Acad Sci 1011:61-8. 2004
    ..TFAM is tightly associated with mtDNA as a main component of the nucleoid...
  9. pmc Architectural role of mitochondrial transcription factor A in maintenance of human mitochondrial DNA
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mol Cell Biol 24:9823-34. 2004
    ..Thus, the mtDNA amount is finely correlated with the amount of TFAM but not with the transcription level. We discuss an architectural role for TFAM in the maintenance of mtDNA in addition to its role in transcription activation...
  10. pmc Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast
    Yusuke Kurihara
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    J Biol Chem 287:3265-72. 2012
    ..Mitophagy contributes to this process by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production...
  11. doi request reprint Reverse of age-dependent memory impairment and mitochondrial DNA damage in microglia by an overexpression of human mitochondrial transcription factor a in mice
    Yoshinori Hayashi
    Laboratory of Oral Aging Science, Faculty of Dental Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Neurosci 28:8624-34. 2008
    ..Together, an overexpression of TFAM is therefore considered to ameliorate age-dependent impairment of the brain functions through the prevention of oxidative stress and mitochondrial dysfunctions in microglia...
  12. pmc Phosphorylation of Serine 114 on Atg32 mediates mitophagy
    Yoshimasa Aoki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Mol Biol Cell 22:3206-17. 2011
    ..We also found that Hog1 and Pbs2, which are involved in the osmoregulatory signal transduction cascade, are related to Atg32 phosphorylation and mitophagy...
  13. doi request reprint Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins
    Shinya Matsumoto
    Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Mitochondrion 12:391-8. 2012
    ..The RNA-binding protein Y-box binding protein-1 is localized at the mitochondrial outer membrane and bound to the OXPHOS mRNAs. Our findings offer new insight into mitochondrial co-translational import in human cells...
  14. pmc p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability
    Mikako Yagi
    Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nucleic Acids Res 40:9717-37. 2012
    ..Co-immunoprecipitation revealed the close association of p32 with the mitoribosome. We propose that p32 is required for functional mitoribosome formation to synthesize proteins within mitochondria...
  15. pmc Protein instability and functional defects caused by mutations of dihydro-orotate dehydrogenase in Miller syndrome patients
    Jingxian Fang
    Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi ku, Fukuoka, Japan
    Biosci Rep 32:631-9. 2012
    ....
  16. doi request reprint Mutation and functional analysis of ABCC2/multidrug resistance protein 2 in a Japanese patient with Dubin-Johnson syndrome
    Takeshi Uchiumi
    Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka
    Hepatol Res 43:569-75. 2013
    ..No MRP2 protein was expressed from HEK293 cells transfected with an R1310X-containing construct. This compound heterozygous mutation of the MRP2 gene causes dysfunction of the MRP2 protein and the hyperbilirubinemia seen in DJS...
  17. doi request reprint Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA
    Shinya Matsumoto
    Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    Biochem J 443:573-84. 2012
    ..Taken together, our findings suggest that YB-1 is a critical factor for translation that may control OXPHOS activity...
  18. pmc A genomic screen for yeast mutants defective in selective mitochondria autophagy
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Mol Biol Cell 20:4730-8. 2009
    ..Accordingly, we have named this gene ATG33. The new mutants identified in this analysis will provide a useful foundation for researchers interested in the study of mitochondrial homeostasis and quality control...
  19. pmc Mitochondria autophagy in yeast
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Antioxid Redox Signal 14:1989-2001. 2011
    ..In this article, we review recent advances in the physiology and molecular mechanism of mitophagy and discuss the similarities and differences of this degradation process between yeast and mammalian cells...
  20. pmc The molecular mechanism of mitochondria autophagy in yeast
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Mol Microbiol 75:795-800. 2010
    ..Here, we review the current knowledge on mitophagy, in particular on the molecular mechanism and regulation of mitophagy in yeast. We also discuss some of the differences between yeast and mammalian mitophagy...
  21. pmc Human mitochondrial DNA is packaged with TFAM
    Tanfis Istiaq Alam
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nucleic Acids Res 31:1640-5. 2003
    ..Based on the fact that TFAM is abundant enough to wrap mtDNA entirely, these results suggest that human mtDNA is packaged with TFAM...
  22. ncbi request reprint The N-terminal region of the transmembrane domain of human erythrocyte band 3. Residues critical for membrane insertion and transport activity
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 278:5564-73. 2003
    ..Finally, deletion of the cytoplasmic surface sequence G(381)LVRD abolished chloride transport, but not surface expression, indicating that this sequence makes an essential structural contribution to the anion transport site of band 3...