Shigetsugu Hatakeyama

Summary

Affiliation: Kyushu University
Country: Japan

Publications

  1. pmc Degradation of p57Kip2 mediated by SCFSkp2-dependent ubiquitylation
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 100:10231-6. 2003
  2. ncbi request reprint U-box proteins as a new family of ubiquitin ligases
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 302:635-45. 2003
  3. ncbi request reprint Ubiquitylation as a quality control system for intracellular proteins
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka
    J Biochem 134:1-8. 2003
  4. pmc Phosphorylation-dependent degradation of c-Myc is mediated by the F-box protein Fbw7
    Masayoshi Yada
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi, Higashi ku, Fukuoka, Fukuoka, Japan
    EMBO J 23:2116-25. 2004
  5. ncbi request reprint Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis
    Fumihiko Okumura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 279:53533-43. 2004
  6. ncbi request reprint Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Nat Cell Biol 6:1229-35. 2004
  7. ncbi request reprint Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Genes Cells 9:533-48. 2004
  8. ncbi request reprint U-box protein carboxyl terminus of Hsc70-interacting protein (CHIP) mediates poly-ubiquitylation preferentially on four-repeat Tau and is involved in neurodegeneration of tauopathy
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Fukuoka, Japan
    J Neurochem 91:299-307. 2004
  9. ncbi request reprint Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein
    Hidehisa Takahashi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 280:5611-21. 2005
  10. pmc Molecular clearance of ataxin-3 is regulated by a mammalian E4
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    EMBO J 23:659-69. 2004

Collaborators

Detail Information

Publications39

  1. pmc Degradation of p57Kip2 mediated by SCFSkp2-dependent ubiquitylation
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 100:10231-6. 2003
    ..These observations thus demonstrate that the SCFSkp2 complex plays an important role in cell-cycle progression by determining the abundance of p57Kip2 and that of the related CDK inhibitor p27Kip1...
  2. ncbi request reprint U-box proteins as a new family of ubiquitin ligases
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 302:635-45. 2003
    ..We here review recent progress in the characterization of U-box proteins and of their role in the quality control system that underlies the cellular stress response to the intracellular accumulation of abnormal proteins...
  3. ncbi request reprint Ubiquitylation as a quality control system for intracellular proteins
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka
    J Biochem 134:1-8. 2003
    ..This review addresses recent progress in characterization of the role of E3 enzymes, especially that of U-box proteins, in quality control of intracellular proteins...
  4. pmc Phosphorylation-dependent degradation of c-Myc is mediated by the F-box protein Fbw7
    Masayoshi Yada
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi, Higashi ku, Fukuoka, Fukuoka, Japan
    EMBO J 23:2116-25. 2004
    ..Accumulation of c-Myc was also apparent in mouse Fbw7-/- embryonic stem cells. These observations suggest that two F-box proteins, Fbw7 and Skp2, differentially regulate c-Myc stability by targeting MB1 and MB2, respectively...
  5. ncbi request reprint Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis
    Fumihiko Okumura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 279:53533-43. 2004
    ..These findings indicate that E4B serves as a ubiquitin ligase for FEZ1 and thereby regulates its function but not its degradation...
  6. ncbi request reprint Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Nat Cell Biol 6:1229-35. 2004
    ..Depletion of KPC1 by RNA interference also inhibited p27(Kip1) degradation. KPC thus probably controls degradation of p27(Kip1) in G1 phase after export of the latter from the nucleus...
  7. ncbi request reprint Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Genes Cells 9:533-48. 2004
    ..These observations suggest that the function of U box-type E3s is to mediate the degradation of unfolded or misfolded proteins in conjunction with molecular chaperones as receptors that recognize such abnormal proteins...
  8. ncbi request reprint U-box protein carboxyl terminus of Hsc70-interacting protein (CHIP) mediates poly-ubiquitylation preferentially on four-repeat Tau and is involved in neurodegeneration of tauopathy
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Fukuoka, Japan
    J Neurochem 91:299-307. 2004
    ..Thus, CHIP is a ubiquitin ligase for four-repeat tau and maintains neuronal survival by regulating the quality control of tau in neurons...
  9. ncbi request reprint Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein
    Hidehisa Takahashi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 280:5611-21. 2005
    ..These results suggest that SUMO-1 functions to tether proteins to PML-containing nuclear bodies through post-translational modification and noncovalent protein-protein interaction...
  10. pmc Molecular clearance of ataxin-3 is regulated by a mammalian E4
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    EMBO J 23:659-69. 2004
    ..These observations suggest that E4 is a rate-limiting factor in the degradation of pathological forms of ataxin-3, and that targeted expression of E4B is a potential gene therapy for SCA3...
  11. ncbi request reprint Large-scale analysis of the human ubiquitin-related proteome
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka, Japan
    Proteomics 5:4145-51. 2005
    ..Our results demonstrate the potential of proteomics analysis of protein ubiquitylation to provide important insight into the regulation of protein stability and other ubiquitin-related cellular functions...
  12. pmc Mammalian E4 is required for cardiac development and maintenance of the nervous system
    Chie Kaneko-Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    Mol Cell Biol 25:10953-64. 2005
    ..UFD2a thus appears to be essential for the development of cardiac muscle, as well as for the protection of spinocerebellar neurons from degeneration induced by endoplasmic reticulum stress...
  13. ncbi request reprint Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways
    Masaki Matsumoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Fukuoka, Japan
    Proteomics 9:3549-63. 2009
    ..Thus comparative and quantitative studies of tyrosine-phosphorylation have the potential to expand knowledge of signaling networks and to promote understanding of signal transduction at the system level...
  14. ncbi request reprint Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular development
    Ryosuke Tsunematsu
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    J Biol Chem 279:9417-23. 2004
    ..Expression of Notch1, -2, or -3 or of cyclin E was unaffected in Fbw7(-/-) embryos. Mammalian Fbw7 thus appears to play an indispensable role in negative regulation of the Notch4-Hey1 pathway and is required for vascular development...
  15. ncbi request reprint Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1
    Kiyotaka Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Japan
    Biochem Biophys Res Commun 303:1209-16. 2003
    ..These observations thus suggest that TIP120A may function as a negative regulator of the SCF complex by binding to nonneddylated Cul1 and thereby preventing assembly of this ubiquitin ligase...
  16. ncbi request reprint Skp2-mediated degradation of p27 regulates progression into mitosis
    Keiko Nakayama
    National Institute of Advanced Industrial Science and Technology AIST, Biological Information Research Center, Tokyo 135 0064, Japan
    Dev Cell 6:661-72. 2004
    ..These data suggest that p27 proteolysis is necessary for the activation of not only Cdk2 but also Cdc2, and that Skp2 contributes to regulation of G(2)-M progression by mediating the degradation of p27...
  17. pmc Impaired degradation of inhibitory subunit of NF-kappa B (I kappa B) and beta-catenin as a result of targeted disruption of the beta-TrCP1 gene
    Keiko Nakayama
    Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 100:8752-7. 2003
    ..In addition, they implicate beta-TrCP1 in the maintenance of ploidy during cell-cycle progression...
  18. ncbi request reprint Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a
    Chie Kaneko
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, 812 8582, Japan
    Biochem Biophys Res Commun 300:297-304. 2003
    ..We also show that UFD2a interacts with VCP (a AAA-family ATPase) that is thought to mediate protein folding. These data implicate UFD2a in the degradation of neuronal proteins by the ubiquitin-proteasome pathway...
  19. pmc Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18
    Ikuo Nakamichi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Mol Biol Cell 13:3441-51. 2002
    ..Deregulated expression of K18, or an imbalance between K8 and K18, may thus be an important determinant of MB formation, which compromises the function of centrosomes and the microtubule network and leads to cell death...
  20. ncbi request reprint Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta
    Akimoto Miyamoto
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Nature 416:865-9. 2002
    ..These results suggest that PKC-delta has an indispensable function in negative regulation of B-cell proliferation, and is particularly important for the establishment of B-cell tolerance...
  21. ncbi request reprint Degradation of Tob1 mediated by SCFSkp2-dependent ubiquitination
    Yoshihiro Hiramatsu
    Second Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Cancer Res 66:8477-83. 2006
    ..Moreover, cyclin D1 expression was suppressed in Skp2 knockdown HeLa cells. These data suggest that Tob1 is a novel target for degradation by the SCF-Skp2 ubiquitin ligase...
  22. ncbi request reprint Ro52 functionally interacts with IgG1 and regulates its quality control via the ERAD system
    Mutsumi Takahata
    Department of Biochemistry, Hokkaido University Graduate School of Medicine, N15 W7, Kita ku, Sapporo, Hokkaido 060 8638, Japan
    Mol Immunol 45:2045-54. 2008
    ..Taken together, our findings suggest that Ro52 plays a significant role in quality control of IgG1 through the ERAD system...
  23. ncbi request reprint Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase
    Rodolfo Murillas
    Experimental Immunology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892 1360, USA
    J Biol Chem 277:2897-907. 2002
    ..However, this protein clearly associates with Nedd4 through its PY domains and can alter the location of Nedd4 in cells, suggesting a role other than as a ubiquitylation substrate...
  24. ncbi request reprint Ubiquitylation and degradation of serum-inducible kinase by hVPS18, a RING-H2 type ubiquitin ligase
    Satomi Yogosawa
    Department of Neurochemistry, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187 8502, Japan
    J Biol Chem 280:41619-27. 2005
    ..Furthermore, the delayed entry into S phase is observed in HeLa cells overexpressing hVPS18. These results suggest that hVPS18 may play an important role in regulation of SNK activity through its ubiquitin ligase...
  25. ncbi request reprint CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity
    Yuzuru Imai
    Laboratory for Motor System Neurodegeneration, RIKEN Brain Science Institute, Saitama 351 0198, Japan
    Mol Cell 10:55-67. 2002
    ..Furthermore, CHIP enhanced the ability of Parkin to inhibit cell death induced by Pael-R. Taken together, these results indicate that CHIP is a mammalian E4-like molecule that positively regulates Parkin E3 activity...
  26. ncbi request reprint [Neurodegenerative diseases regulated by ubiquitin-proteasome system]
    Shigetsugu Hatakeyama
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, Kita ku, Sapporo, Japan
    Seikagaku 77:389-99. 2005
  27. ncbi request reprint CHIP promotes proteasomal degradation of familial ALS-linked mutant SOD1 by ubiquitinating Hsp/Hsc70
    Makoto Urushitani
    Laboratory for Motor System Neurodegeneration, RIKEN Brain Science Institute, Wako, Saitama, Japan
    J Neurochem 90:231-44. 2004
    ..Taken together, we propose a novel pathway in which ubiquitinated Hsp/Hsc70 might deliver mutant SOD1 to, and facilitate its degradation, at the proteasome...
  28. ncbi request reprint Subcellular expression of autoimmune regulator is organized in a spatiotemporal manner
    Hiroko Akiyoshi
    Division of Molecular Immunology, Institute for Enzyme Research, University of Tokushima, Tokushima 770 8503, Japan
    J Biol Chem 279:33984-91. 2004
    ..These results suggest that the transcriptional regulating activities of AIRE within a cell are controlled and organized in a spatiotemporal manner...
  29. ncbi request reprint Targeted destruction of c-Myc by an engineered ubiquitin ligase suppresses cell transformation and tumor formation
    Shigetsugu Hatakeyama
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Cancer Res 65:7874-9. 2005
    ..These findings indicate that the targeted destruction of c-Myc by an artificial E3 may represent an effective therapeutic strategy for certain human malignancies...
  30. doi request reprint Tripartite motif protein 32 facilitates cell growth and migration via degradation of Abl-interactor 2
    Satoshi Kano
    Department of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan
    Cancer Res 68:5572-80. 2008
    ..These findings suggest that TRIM32 is a novel oncogene that promotes tumor growth, metastasis, and resistance to anticancer drugs...
  31. doi request reprint TRIM68 regulates ligand-dependent transcription of androgen receptor in prostate cancer cells
    Naoto Miyajima
    Department of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Cancer Res 68:3486-94. 2008
    ..These results indicate that TRIM68 functions as a cofactor for AR-mediated transcription and is likely to be a novel diagnostic tool and a potentially therapeutic target for prostate cancer...
  32. ncbi request reprint Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA
    Satoru Maruyama
    Department of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan
    Mol Cell Biochem 307:73-82. 2008
    ..Our results indicate that PIRH2 functions as a regulator for COP I complex...
  33. ncbi request reprint Involvement of Rabring7 in EGF receptor degradation as an E3 ligase
    Ayuko Sakane
    Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770 8503, Japan
    Biochem Biophys Res Commun 357:1058-64. 2007
    ..These results suggest that Rabring7 is involved in the endocytic trafficking of EGFR through its E3 ligase activity...
  34. ncbi request reprint [Neurodegenerative diseases regulated by ubiquitin-proteasome system]
    Shigetsugu Hatakeyama
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine
    Rinsho Shinkeigaku 46:890-2. 2006
    ..These observations suggest that E4 is a rate-limiting factor in the degradation of proteins with expanded polyglutamine tracts...
  35. ncbi request reprint Ligand-dependent transcription of estrogen receptor alpha is mediated by the ubiquitin ligase EFP
    Ayako Nakajima
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, Kita ku, Sapporo, Hokkaido 060 8638, Japan
    Biochem Biophys Res Commun 357:245-51. 2007
    ..Our results indicate that EFP functions as a cofactor for ERalpha-mediated transcription, thus suggesting that ERalpha-mediated transcription is closely linked to the ubiquitylation of ERalpha...
  36. ncbi request reprint APS-mediated ubiquitination of the insulin receptor enhances its internalization, but does not induce its degradation
    Kazuhiro Kishi
    Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima, Japan
    Endocr J 54:77-88. 2007
    ..APS-mediated ubiquitination of the IR induced enhancement of the IR internalization, but did not affect the IR degradation. This finding shows one of the pleiotropic functions of APS in insulin signaling...
  37. ncbi request reprint Elmo1 inhibits ubiquitylation of Dock180
    Yoshinori Makino
    Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, N15, W7, Sapporo 060 8638, Japan
    J Cell Sci 119:923-32. 2006
    ..Therefore, an ubiquitin-proteasome-dependent protein degradation mechanism might contribute to the local activation of Rac on the plasma membrane...
  38. ncbi request reprint Mapping of ubiquitination sites on target proteins
    Shigetsugu Hatakeyama
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Methods Enzymol 399:277-86. 2005
    ..Using these techniques, we can obtain comprehensive information about ubiquitinated proteins in various cells and tissues...
  39. pmc AIRE functions as an E3 ubiquitin ligase
    Daisuke Uchida
    Division of Molecular Immunology, Institute for Enzyme Research, University of Tokushima, 3 18 15 Kuramoto, Tokushima 770 8503, Japan
    J Exp Med 199:167-72. 2004
    ..These results add a novel enzymatic function for AIRE and suggest an indispensable role of the Ub proteasome pathway in the establishment of self-tolerance, in which AIRE is involved...