Kiyoshi Yasukawa

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. Yasukawa K, Mizuno M, Inouye K. Characterization of Moloney murine leukaemia virus/avian myeloblastosis virus chimeric reverse transcriptases. J Biochem. 2009;145:315-24 pubmed publisher
    ....
  2. Katano Y, Hisayoshi T, Kuze I, Okano H, Ito M, Nishigaki K, et al. Expression of moloney murine leukemia virus reverse transcriptase in a cell-free protein expression system. Biotechnol Lett. 2016;38:1203-11 pubmed publisher
    ..MMLV RT expressed in the cell-free system is indistinguishable from that expressed in E. coli. ..
  3. Okano H, Katano Y, Baba M, Fujiwara A, Hidese R, Fujiwara S, et al. Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase. Enzyme Microb Technol. 2017;96:111-120 pubmed publisher
    ..Our results suggest that DNA polymerase with RT activity and DNA/RNA helicase are useful to increase the sensitivity of cDNA synthesis. ..
  4. request reprint
    Yasukawa K, Nemoto D, Inouye K. Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and Moloney murine leukaemia virus. J Biochem. 2008;143:261-8 pubmed
    ..Thermodynamic analysis indicates that thermal inactivation of AMV RT and MMLV RT is due to the large entropy change of activation for thermal inactivation...
  5. Yasukawa K, Iida K, Okano H, Hidese R, Baba M, Yanagihara I, et al. Next-generation sequencing-based analysis of reverse transcriptase fidelity. Biochem Biophys Res Commun. 2017;492:147-153 pubmed publisher
    ..Overall, our method could precisely evaluate the fidelity of various RTs with different reaction conditions in a high-throughput manner without the use of expensive optics and troublesome adaptor ligation. ..
  6. Okano H, Baba M, Kawato K, Hidese R, Yanagihara I, Kojima K, et al. High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies. J Biosci Bioeng. 2017;: pubmed publisher
    ..Considering that K4polL329A and RTX are stable even at 90-100°C, our results suggest that the one-step RT-PCR with K4polL329A or RTX is more advantageous than the current one...

Locale

Detail Information

Publications6

  1. Yasukawa K, Mizuno M, Inouye K. Characterization of Moloney murine leukaemia virus/avian myeloblastosis virus chimeric reverse transcriptases. J Biochem. 2009;145:315-24 pubmed publisher
    ....
  2. Katano Y, Hisayoshi T, Kuze I, Okano H, Ito M, Nishigaki K, et al. Expression of moloney murine leukemia virus reverse transcriptase in a cell-free protein expression system. Biotechnol Lett. 2016;38:1203-11 pubmed publisher
    ..MMLV RT expressed in the cell-free system is indistinguishable from that expressed in E. coli. ..
  3. Okano H, Katano Y, Baba M, Fujiwara A, Hidese R, Fujiwara S, et al. Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase. Enzyme Microb Technol. 2017;96:111-120 pubmed publisher
    ..Our results suggest that DNA polymerase with RT activity and DNA/RNA helicase are useful to increase the sensitivity of cDNA synthesis. ..
  4. request reprint
    Yasukawa K, Nemoto D, Inouye K. Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and Moloney murine leukaemia virus. J Biochem. 2008;143:261-8 pubmed
    ..Thermodynamic analysis indicates that thermal inactivation of AMV RT and MMLV RT is due to the large entropy change of activation for thermal inactivation...
  5. Yasukawa K, Iida K, Okano H, Hidese R, Baba M, Yanagihara I, et al. Next-generation sequencing-based analysis of reverse transcriptase fidelity. Biochem Biophys Res Commun. 2017;492:147-153 pubmed publisher
    ..Overall, our method could precisely evaluate the fidelity of various RTs with different reaction conditions in a high-throughput manner without the use of expensive optics and troublesome adaptor ligation. ..
  6. Okano H, Baba M, Kawato K, Hidese R, Yanagihara I, Kojima K, et al. High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies. J Biosci Bioeng. 2017;: pubmed publisher
    ..Considering that K4polL329A and RTX are stable even at 90-100°C, our results suggest that the one-step RT-PCR with K4polL329A or RTX is more advantageous than the current one...