Mitsuhiro Yanagida

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Fission yeast cut mutations revisited: control of anaphase
    M Yanagida
    Dept of Biophysics, Graduate School of Science, Kyoto University, Japan
    Trends Cell Biol 8:144-9. 1998
  2. ncbi request reprint From phage to chromosome biology: a personal account
    M Yanagida
    CREST Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho Sakyoku, Kyoto, 606 8502, Japan
    J Mol Biol 293:181-5. 1999
  3. pmc Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization
    M Yanagida
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Japan
    Philos Trans R Soc Lond B Biol Sci 354:1559-69; discussion 1569-70. 1999
  4. pmc The model unicellular eukaryote, Schizosaccharomyces pombe
    Mitsuhiro Yanagida
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Genome Biol 3:COMMENT2003. 2002
  5. pmc Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis
    Norihiko Nakazawa
    Core Research for Evolutional Science and Technology Research Program, Japan Science and Technology Corporation, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 180:1115-31. 2008
  6. doi request reprint Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin
    Yuu Kimata
    CREST Research Program, Japan Science and Technology Corporation, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    J Cell Sci 121:1107-18. 2008
  7. pmc Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes
    Tomomi Kiyomitsu
    Core Research for Evolutional Science and Technology Research Program, Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 188:791-807. 2010
  8. ncbi request reprint An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation
    Tatsuro Yuasa
    Department of Biophysics, Graduate School of Science, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Genes Cells 9:1069-82. 2004
  9. ncbi request reprint Separase-mediated cleavage of cohesin at interphase is required for DNA repair
    Koji Nagao
    Department of Biophysics, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nature 430:1044-8. 2004
  10. pmc Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin
    Ee Sin Chen
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 101:8078-83. 2004

Collaborators

Detail Information

Publications58

  1. ncbi request reprint Fission yeast cut mutations revisited: control of anaphase
    M Yanagida
    Dept of Biophysics, Graduate School of Science, Kyoto University, Japan
    Trends Cell Biol 8:144-9. 1998
    ....
  2. ncbi request reprint From phage to chromosome biology: a personal account
    M Yanagida
    CREST Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho Sakyoku, Kyoto, 606 8502, Japan
    J Mol Biol 293:181-5. 1999
    ..A personal account from phage morphogenesis to chromosome biology is described in this review...
  3. pmc Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization
    M Yanagida
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Japan
    Philos Trans R Soc Lond B Biol Sci 354:1559-69; discussion 1569-70. 1999
    ..Cdc13 enriched along the spindle disappears first, followed by decay of the nuclear signal, whereas Cut2 in the nucleus disappears first, followed by decline in the spindle signal during metaphase-anaphase progression...
  4. pmc The model unicellular eukaryote, Schizosaccharomyces pombe
    Mitsuhiro Yanagida
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Genome Biol 3:COMMENT2003. 2002
    ..Now that its genome has been sequenced, S. pombe is ready to assume its rightful place in the pantheon of small eukaryotic giants...
  5. pmc Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis
    Norihiko Nakazawa
    Core Research for Evolutional Science and Technology Research Program, Japan Science and Technology Corporation, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 180:1115-31. 2008
    ..The mechanism of condensin accumulation at the kinetochores may be conserved, as human condensin II fails to accumulate at kinetochores in hMis6 RNA interference-treated cells...
  6. doi request reprint Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin
    Yuu Kimata
    CREST Research Program, Japan Science and Technology Corporation, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    J Cell Sci 121:1107-18. 2008
    ..HATs and HDACs might directly target adherin and APC/C to ensure proper chromosome segregation, and anti-tumour effects of HDAC inhibitors could be attributed to this deregulation...
  7. pmc Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes
    Tomomi Kiyomitsu
    Core Research for Evolutional Science and Technology Research Program, Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 188:791-807. 2010
    ..hMis14, which contains a tripartite-binding domain for HP1 and two other kinetochore proteins, hMis13 and blinkin, is a cornerstone for the assembly of the inner centromere and kinetochore...
  8. ncbi request reprint An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation
    Tatsuro Yuasa
    Department of Biophysics, Graduate School of Science, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Genes Cells 9:1069-82. 2004
    ..pombe cdc48 mutants displayed the mitotic phenotype highly similar to separase/cut1 mutants. This approach also provides a novel way of mutant isolation, resulting in two histone H2B strains and a cohesion mutant with a new phenotype...
  9. ncbi request reprint Separase-mediated cleavage of cohesin at interphase is required for DNA repair
    Koji Nagao
    Department of Biophysics, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nature 430:1044-8. 2004
    ..We propose that the securin-separase complex might aid DNA repair by removing local cohesin in interphase cells...
  10. pmc Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin
    Ee Sin Chen
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 101:8078-83. 2004
    ..Cti1 is the first example of the protein that interacts with the hinge domain of SMC. Cti1 may have a supporting role for the DNA repair function of condensin...
  11. ncbi request reprint Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres
    Takeshi Hayashi
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, 606 8502, Japan
    Cell 118:715-29. 2004
    ..RNAi knockdown in human cells shows that Mis16 function is conserved as RbAp48 and RbAp46 are both required for localization of human CENP-A...
  12. pmc Protein interaction domain mapping of human kinetochore protein Blinkin reveals a consensus motif for binding of spindle assembly checkpoint proteins Bub1 and BubR1
    Tomomi Kiyomitsu
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Mol Cell Biol 31:998-1011. 2011
    ..Taken together, these results indicate that blinkin functions to connect Bub1 and BubR1 with the hMis12, Ndc80, and Zwint-1 complexes, and disruption of this connection may lead to tumorigenesis...
  13. ncbi request reprint Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1
    Tomomi Kiyomitsu
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Dev Cell 13:663-76. 2007
    ..Blinkin may be the center of the network for generating kinetochore-based checkpoint signaling...
  14. pmc Basic mechanism of eukaryotic chromosome segregation
    Mitsuhiro Yanagida
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Philos Trans R Soc Lond B Biol Sci 360:609-21. 2005
    ..The roles of principal players in basic chromosome segregation are discussed: most players have interphase as well as mitotic functions. A view on how the centromere/kinetochore is formed is described...
  15. doi request reprint Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor
    Yuichiro Hanyu
    CREST Research Project, Japan Science Technology Corporation, Kyoto, Japan
    Genes Cells 14:539-54. 2009
    ..Ssp1 and Ppe1 thus seem to antagonize in utilizing limited glucose. We also show that Ppk9 and Ssp2 are the catalytic subunits of AMPK and AMPK-related kinases, respectively, which bind to common beta-(Amk2) and gamma-(Cbs2) subunits...
  16. pmc Coordinated requirements of human topo II and cohesin for metaphase centromere alignment under Mad2-dependent spindle checkpoint surveillance
    Yusuke Toyoda
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Mol Biol Cell 17:2287-302. 2006
    ..Cohesin and topo II have distinct, yet coordinated functions in metaphase alignment...
  17. ncbi request reprint A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1
    Chikashi Obuse
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nat Cell Biol 6:1135-41. 2004
    ..Double HP1 RNAi abolishes kinetochore localization of hMis12 and DC8. Therefore, centromeric HP1 may be the base to anchor the hMis12 core complex that is enriched with coiled coils and extends to outer Zwint-1 during mitosis...
  18. pmc The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore
    Gohta Goshima
    COE Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502 Japan
    EMBO J 22:2752-63. 2003
    ..Ppe1/PP6 directly or indirectly modulates kinetochore chromatin protein Mis12 to ensure progression into normal anaphase...
  19. pmc Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation
    Takeshi Maruyama
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto, Japan
    EMBO J 25:2420-31. 2006
    ..Histone H2B thus plays an important role in centromere/kinetochore formation...
  20. ncbi request reprint Fission yeast MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses
    Yosuke Kawasaki
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    Cell Cycle 5:1831-9. 2006
    ..Securin bound to separase was hyperphosphorylated. The stresses could not rescue the indestructible Cut2 and Rad21 mutants. We postulate that the stresses produce the hyperchaperonic form of Cut2 that can rescue separase mutations...
  21. ncbi request reprint Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1
    Yohta Fujita
    CREST Research Project of Japan Science and Technology Corporation JST, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    Dev Cell 12:17-30. 2007
    ..Telophase centromere chromatin may be primed or licensed by the hMis18 complex and RbAp46/48 to recruit CENP-A through regulating the acetylation status in the centromere...
  22. ncbi request reprint Cdc48 is required for the stability of Cut1/separase in mitotic anaphase
    Nobuyasu Ikai
    Department of Gene Mechanism, Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    J Struct Biol 156:50-61. 2006
    ..Cells lose viability during the traverse of anaphase in cdc48 mutant cells. Cdc48 may protect Cut1/separase and Cut2/securin against the instability during polyubiquitination and degradation in the metaphase-anaphase transition...
  23. ncbi request reprint CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation
    Keita Aoki
    Core Research for Evolutional Science and Technology Research Program of Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Curr Biol 16:1627-35. 2006
    ..Cdc2 thus directly phosphorylates Dis1, and this phosphorylation regulates Dis1 localization in both metaphase and anaphase and ensures high-fidelity segregation...
  24. pmc Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast
    Yoshiharu Shiroiwa
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    PLoS ONE 6:e17761. 2011
    ..The Mis6-Mal2-Sim4 complex of the fission yeast S. pombe is required for the recruitment of CenH3 (Cnp1), but its function remains obscure...
  25. ncbi request reprint Cut1/separase-dependent roles of multiple phosphorylation of fission yeast cohesion subunit Rad21 in post-replicative damage repair and mitosis
    Yoh Adachi
    CREST Research Program, Japan Science and Technology Corporation, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto, Japan
    Cell Cycle 7:765-76. 2008
    ..These complex phospho-regulations of Rad21 indicate the functional significance of cohesin in cell adaptation to a variety of cellular conditions...
  26. pmc Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly
    Akiko Sakai
    Department of Biophysics, Graduate School of Science, Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
    EMBO J 22:2764-75. 2003
    ..SMC is eliminated from the completed double-stranded DNA. The ability of heterodimeric SMC to reanneal DNA may be regulated in vivo possibly through the non-SMC heterotrimeric complex...
  27. pmc Regulation of checkpoint kinases through dynamic interaction with Crb2
    Satoru Mochida
    Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto, Japan
    EMBO J 23:418-28. 2004
    ..Hyperphosphorylation of Crb2 is also dependent on its intact BRCT. Finally, we show direct interaction between Rad3 and Crb2, which is inhibitory to Rad3 activity. Hence, Crb2 is the first to interact with both Rad3 and Chk1 kinases...
  28. pmc Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function
    Hiroaki Murakami
    CREST Research Program, Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto, Japan
    PLoS ONE 2:e317. 2007
    ..Previous isolation of the dis3-54 cold-sensitive mutant of fission yeast Schizosaccharomyces pombe suggested that Dis3 is also required for correct chromosome segregation...
  29. ncbi request reprint Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis
    Yuu Kimata
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Sci 117:2283-93. 2004
    ..The previously identified scn1(+) (renamed as scn3(+)) turned out to be a high copy suppressor for scn1 and scn2. These are the first tRNA mutants that cause a mitotic defect...
  30. ncbi request reprint Does a GATA factor make the bed for centromeric nucleosomes?
    Ee Sin Chen
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    Cell Cycle 2:277-8. 2003
    ..Transcription and/or remodeling of the nucleosomes at the centromeres may be important for the precise incorporation of CENP-A in fission yeast...
  31. ncbi request reprint A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids
    Takuhiro Matsumura
    Department of Biophysics, Graduate School of Science and Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Genes Cells 8:341-55. 2003
    ..We intended to identify ts (temperature sensitive) strains whose growth was inhibited by multicopy plasmid pCUT1 or pCUT2 at the permissive temperature...
  32. ncbi request reprint A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast
    Ee Sin Chen
    Department of Biophysics, Graduate School of Science, Kyoto 606 8502, Japan
    Mol Cell 11:175-87. 2003
    ..Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function...
  33. pmc Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway
    Gohta Goshima
    COE Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
    J Cell Biol 160:25-39. 2003
    ..RNAi for hMis6, like that of a kinetochore kinesin CENP-E, induces mitotic arrest. Kinetochore localization of hMis12 is unaffected by CENP-A RNAi, demonstrating an independent pathway of CENP-A in human kinetochores...
  34. ncbi request reprint Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8
    Kojiro Takeda
    Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Cell 122:393-405. 2005
    ..The short-lived nature of Cut8 is crucial for feedback enrichment of the proteasome within the nucleus. This is likely to be a conserved mechanism as we describe a Cut8 homolog in flies...
  35. ncbi request reprint Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation
    Takahiro Nakamura
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502 Japan
    Genes Cells 7:1113-24. 2002
    ....
  36. ncbi request reprint Cnd2 has dual roles in mitotic condensation and interphase
    Nobuki Aono
    Graduate School of Biostatistics, Department of Gene Mechanisms, Kyoto University, Japan
    Nature 417:197-202. 2002
    ..These interphase and mitotic defects provide insight into the mechanistic role of non-SMC subunits that interact with the globular SMC domains in the heteropentameric holocomplex...
  37. doi request reprint Clearing the way for mitosis: is cohesin a target?
    Mitsuhiro Yanagida
    Mitsuhiro Yanagida is at the CREST Research Program, Japan Science Technology Corporation, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Nat Rev Mol Cell Biol 10:489-96. 2009
    ..I propose that condensin and DNA topoisomerase II (TOP2), as well as separase, help to clear the way for mitosis...
  38. ncbi request reprint Heads and tails
    Mitsuhiro Yanagida
    Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    Nature 429:135. 2004
  39. doi request reprint The dream of every chromosome: equal segregation for a healthy life of the host
    Tomohiro Matsumoto
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    Adv Exp Med Biol 570:281-310. 2005
  40. doi request reprint Cellular quiescence: are controlling genes conserved?
    Mitsuhiro Yanagida
    Okinawa Institute of Science and Technology Promotion Corporation OISTPC, Initial Research Project IRP, Uruma, Okinawa 904 2234, Japan
    Trends Cell Biol 19:705-15. 2009
    ..These latest findings suggest that genes controlling quiescence are conserved from yeast to humans, and support the use of S. pombe as a model to enhance our understanding of the causes of aging, diabetes, obesity and neurodegeneration...
  41. ncbi request reprint Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast
    Yusuke Toyoda
    Department of Gene Mechanisms, Graduate School of Biostudies, 606 8502, Kyoto, Japan
    Curr Biol 12:347-58. 2002
    ..The failure to form cohesion in the S phase results in premature separation of chromatids in G2/M. Mitotic kinetochores free from microtubules or the lack of tension are known to activate spindle checkpoint...
  42. ncbi request reprint Tel2: a common partner of PIK-related kinases and a link between DNA checkpoint and nutritional response?
    Junko Kanoh
    Graduate School of Biostudies, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Genes Cells 12:1301-4. 2007
    ..In this minireview, implications and impact of the findings, and a possibility that PIKKs are functionally related through Tel2, are discussed...
  43. ncbi request reprint Condensin architecture and interaction with DNA: regulatory non-SMC subunits bind to the head of SMC heterodimer
    Shige H Yoshimura
    CREST Research Project, Department of Responses to Environmental Signals and Stresses, Graduate School of Biostudies, Kyoto University, Japan
    Curr Biol 12:508-13. 2002
    ..Thus, non-SMC trimers may regulate the ATPase activity of condensin by directly interacting with the globular domains of SMC heterodimer and alter the mode of DNA interaction. A model for the action of heteropentamer is presented...
  44. pmc Introduction: dissection and simplification of chromosome segregation
    Mitsuhiro Yanagida
    Department of Gene Mechanism, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    Philos Trans R Soc Lond B Biol Sci 360:481. 2005
  45. ncbi request reprint Regulating sister chromatid separation by separase phosphorylation
    Koji Nagao
    Department of Biophysics, Graduate School of Science, Kyoto University, 606 8502 Kyoto, Japan
    Dev Cell 2:2-4. 2002
    ..A recent paper in Cell highlights a second mechanism of regulation of separase in addition to the bound inhibitor securin. This second pathway involves separase phosphorylation and is dependent on CDC2...
  46. ncbi request reprint Control of localization of a spindle checkpoint protein, Mad2, in fission yeast
    Amy E Ikui
    Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Cell Sci 115:1603-10. 2002
    ..This localization was abolished in a yeast strain lacking Mad1, a protein that physically interacts with Mad2. Mad1 may anchor Mad2 to the nuclear membrane and regulate its entry into the nucleus...
  47. pmc Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis
    Karen M May
    The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK
    J Cell Biol 156:23-8. 2002
    ..This arrest can be rescued by high expression of Plo1 kinase. We suggest that this physical interaction is crucial for mitotic progression by targeting polo kinase activity toward the APC...
  48. ncbi request reprint Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe
    Mizuki Shimanuki
    Initial Research Project IRP, Okinawa Institute of Science and Technology Promotion Corporation OIST, Uruma 904 2234, Okinawa, Japan
    Genes Cells 12:677-92. 2007
    ....
  49. ncbi request reprint Rapamycin sensitivity of the Schizosaccharomyces pombe tor2 mutant and organization of two highly phosphorylated TOR complexes by specific and common subunits
    Takeshi Hayashi
    The G0 Cell Unit, Okinawa Institute of Science and Technology Promotion Corporation, Suzaki 12 22, Uruma, Okinawa 904 2234, Japan
    Genes Cells 12:1357-70. 2007
    ..Functional redundancy of the TORCs may explain the rapamycin hypersensitivity of tor2-287...
  50. ncbi request reprint The nucleolus is involved in mRNA export from the nucleus in fission yeast
    Takashi Ideue
    Department of Biology, Graduate School of Sciences, Kyushu University, Fukuoka 812 8581, Japan
    J Cell Sci 117:2887-95. 2004
    ..These findings support the notion that a subset of mRNAs in yeast is exported from the nucleus through transient association with the nucleolus...
  51. ncbi request reprint [How does fission yeast cut8 regulate the nuclear localization of proteasome?]
    Kijiro Takeda
    Tanpakushitsu Kakusan Koso 51:1241-4. 2006
  52. ncbi request reprint Two budding yeast RAD4 homologs in fission yeast play different roles in the repair of UV-induced DNA damage
    Yasunori Fukumoto
    Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    DNA Repair (Amst) 1:833-45. 2002
    ..Rhp4B in contrast acts as an accessory protein in non-transcribed strand (NTS) repair...
  53. ncbi request reprint Distinct modes of DNA damage response in S. pombe G0 and vegetative cells
    Satoru Mochida
    The G0 Cell Unit, Initial Research Project IRP, Okinawa Institute of Science and Technology OIST Corporation, 12 22 Suzaki, Uruma, Okinawa 904 2234, Japan
    Genes Cells 11:13-27. 2006
    ..S. pombe G0 cells upon DNA damages thus respond distinctively from VE cells in regard with regulation of checkpoint proteins and the mode of repair that is dependent upon the use of NHEJ...
  54. pmc The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase
    Kazuki Kumada
    Japanese Foundation for Cancer Research, Cancer Institute, Tokyo 135 8550, Japan
    J Cell Biol 172:835-46. 2006
    ..We also demonstrated that heterozygous separase-deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin, which is an inhibitory partner of separase...
  55. ncbi request reprint Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase
    Koji Nagao
    Initial Research Project, Okinawa Institute of Science and Technology, Suzaki 12 22, Uruma, Okinawa, 904 2234, Japan
    Genes Cells 11:247-60. 2006
    ....
  56. pmc Characterization of Schistosoma mansoni Sds homologue, a leucine-rich repeat protein that interacts with protein phosphatase type 1 and interrupts a G2/M cell-cycle checkpoint
    Wassim Daher
    Unité Inserm 547 IPL, Institut Pasteur, 1 rue du Pr Calmette, B P 245, F 59019 Lille cedex, France
    Biochem J 395:433-41. 2006
    ..Similar results showing the appearance of GVBD were observed when oocytes were treated with anti-PP1 antibodies. Taken together, these observations suggest that SmSds can regulate the cell cycle by binding to PP1...
  57. ncbi request reprint The kic1 kinase of schizosaccharomyces pombe is a CLK/STY orthologue that regulates cell-cell separation
    Zhaohua Tang
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Exp Cell Res 283:101-15. 2003
    ....
  58. ncbi request reprint Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant
    Kazuaki Yokoyama
    Faculty of Pharmaceutical Sciences, Teikyo University, Suarashi, Sagamiko machi, Sagamihara shi, Kanagawa, Japan
    J Biochem 143:369-75. 2008
    ..The amounts of VLCFA-PL that accumulated in lsd90-overexpressing lsd1/fas2 cells were significantly lower than those in lsd1/fas2 cells, suggesting the involvement of Lsd90 in the metabolism of VLCFA-PL...