Shinya Yamanaka

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells
    Masayoshi Maruyama
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 280:24371-9. 2005
  2. pmc ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency
    Kumiko A Iwabuchi
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e20461. 2011
  3. doi request reprint Induced pluripotent stem cells: past, present, and future
    Shinya Yamanaka
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 10:678-84. 2012
  4. doi request reprint Elite and stochastic models for induced pluripotent stem cell generation
    Shinya Yamanaka
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:49-52. 2009
  5. doi request reprint Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
  6. pmc Suppression of induced pluripotent stem cell generation by the p53-p21 pathway
    Hyenjong Hong
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:1132-5. 2009
  7. doi request reprint Cell line-dependent differentiation of induced pluripotent stem cells into cardiomyocytes in mice
    Shinji Kaichi
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Cardiovasc Res 88:314-23. 2010
  8. doi request reprint Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
  9. pmc Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells
    Masanori Imamura
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    BMC Dev Biol 6:34. 2006
  10. doi request reprint Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts
    Noriko Tsubooka
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Genes Cells 14:683-94. 2009

Detail Information

Publications71

  1. ncbi request reprint Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells
    Masayoshi Maruyama
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 280:24371-9. 2005
    ..Taken together, these data demonstrate differential roles for Sox15 and Sox2 in transcriptional control in mouse ES cells...
  2. pmc ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency
    Kumiko A Iwabuchi
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e20461. 2011
    ..However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression...
  3. doi request reprint Induced pluripotent stem cells: past, present, and future
    Shinya Yamanaka
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 10:678-84. 2012
    ..However, there is still debate about whether iPSCs are functionally equivalent to ESCs. This question should be answered only by science, not by politics or business...
  4. doi request reprint Elite and stochastic models for induced pluripotent stem cell generation
    Shinya Yamanaka
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:49-52. 2009
    ..Here I consider reasons for bottlenecks in induced pluripotent stem cell generation, and propose a model in which most or all cells have the potential to become pluripotent...
  5. doi request reprint Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  6. pmc Suppression of induced pluripotent stem cell generation by the p53-p21 pathway
    Hyenjong Hong
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:1132-5. 2009
    ..Functional analyses of these genes demonstrate that the p53-p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation...
  7. doi request reprint Cell line-dependent differentiation of induced pluripotent stem cells into cardiomyocytes in mice
    Shinji Kaichi
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Cardiovasc Res 88:314-23. 2010
    ..iPS cells can be generated even without the expression of c-Myc. The present study examined patterns of differentiation of mouse iPS cells into cardiomyocytes in three different cell lines reprogrammed by three or four factors...
  8. doi request reprint Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
    ..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine...
  9. pmc Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells
    Masanori Imamura
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    BMC Dev Biol 6:34. 2006
    ..We previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells...
  10. doi request reprint Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts
    Noriko Tsubooka
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Genes Cells 14:683-94. 2009
    ..These results demonstrated that Sall4 plays positive roles in the generation of pluripotent stem cells from blastocysts and fibroblasts...
  11. ncbi request reprint Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
    ..These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors...
  12. doi request reprint Variation in the safety of induced pluripotent stem cell lines
    Kyoko Miura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Nat Biotechnol 27:743-5. 2009
    ..In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells...
  13. doi request reprint Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
    Shogo Nagata
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 14:1395-404. 2009
    ....
  14. ncbi request reprint Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    ..Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application...
  15. ncbi request reprint Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    ..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...
  16. ncbi request reprint Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
    ..Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts...
  17. ncbi request reprint Utilization of digital differential display to identify novel targets of Oct3/4
    Yoshimi Tokuzawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Methods Mol Biol 329:223-31. 2006
    ..Reporter gene and gel mobility shift assays are used to confirm the role of Oct3/4. Identification of novel Oct3/4 targets will facilitate our understanding of pluripotency...
  18. pmc Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells
    Michiyo Koyanagi-Aoi
    Center for iPS Cell Research and Application, Department of Biological Repair, Institute for Frontier Medical Sciences, and Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 110:20569-74. 2013
    ..These data demonstrated a subset of hiPSC lines that have aberrant gene expression and defective potential in neural differentiation, which need to be identified and eliminated before applications in regenerative medicine. ..
  19. doi request reprint A more efficient method to generate integration-free human iPS cells
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Nat Methods 8:409-12. 2011
    ..This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future...
  20. doi request reprint Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Aβ and differential drug responsiveness
    Takayuki Kondo
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 12:487-96. 2013
    ..It also illustrates how patient-specific iPSCs can be useful for analyzing AD pathogenesis and evaluating drugs...
  21. doi request reprint Response to Comment on "Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells"
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 5:188lr2. 2013
    ..Our work and the study of Bilican et al. highlight the need for complementary assays to detect subtle phenotypic differences between control and mutant induced pluripotent stem cell lines...
  22. doi request reprint Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells
    Koichiro Homma
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Atherosclerosis 212:42-7. 2010
    ..Here, we compared the functionality of human adult ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs)...
  23. doi request reprint Generation of mouse-induced pluripotent stem cells with plasmid vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto, Japan
    Nat Protoc 5:418-28. 2010
    ..This virus-free technique reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency...
  24. doi request reprint An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells
    Keisuke Okita
    Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Stem Cells 31:458-66. 2013
    ..This induction method will be useful for the derivation of patient-specific integration-free iPSCs and would also be applicable to the generation of clinical-grade iPSCs in the future...
  25. doi request reprint Generation and characterization of human induced pluripotent stem cells
    Mari Ohnuki
    Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto, Japan
    Curr Protoc Stem Cell Biol . 2009
    ..RT-PCR and immunocytochemistry for detection of pluripotent cell markers, embryoid body differentiation, and teratoma differentiation are used to determine pluripotency in vitro and in vivo, respectively...
  26. doi request reprint Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1
    Momoko Maekawa
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Nature 474:225-9. 2011
    ..These results therefore show that Glis1 effectively promotes the direct reprogramming of somatic cells during iPSC generation...
  27. pmc Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression
    Hideki Uosaki
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e23657. 2011
    ..In this study, we attempted to identify cell surface markers for cardiomyocytes derived from hESC/hiPSCs...
  28. doi request reprint The effects of cardioactive drugs on cardiomyocytes derived from human induced pluripotent stem cells
    Noritaka Yokoo
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto, Japan
    Biochem Biophys Res Commun 387:482-8. 2009
    ..Thus, human iPS cells could become an attractive tool to investigate the effects of cardioactive drugs at the individual level and to screen for individually tailored drugs against cardiac arrhythmic diseases...
  29. pmc Promotion of direct reprogramming by transformation-deficient Myc
    Masato Nakagawa
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 107:14152-7. 2010
    ..These data demonstrate that different functional moieties of the Myc proto-oncogene products are involved in the transformation and promotion of directed reprogramming...
  30. doi request reprint Orderly hematopoietic development of induced pluripotent stem cells via Flk-1(+) hemoangiogenic progenitors
    Akira Niwa
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Cell Physiol 221:367-77. 2009
    ..This finding highlights the potential use of iPS cells in clinical areas such as regenerative medicine, disease investigation, and drug screening...
  31. doi request reprint Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells
    Daisuke Taura
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8507, Japan
    FEBS Lett 583:1029-33. 2009
    ..These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells...
  32. ncbi request reprint Induction of pluripotent stem cells from fibroblast cultures
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Protoc 2:3081-9. 2007
    ..The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells...
  33. doi request reprint Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells
    Shin Ichi Mae
    Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Commun 4:1367. 2013
    ....
  34. doi request reprint Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm
    Kazutoshi Takahashi
    1 Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan 2
    Nat Commun 5:3678. 2014
    ..These results demonstrate that during the reprogramming process, human somatic cells go through a transient state that resembles mesendoderm. ..
  35. pmc Essential roles of ECAT15-2/Dppa2 in functional lung development
    Tomonori Nakamura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Mol Cell Biol 31:4366-78. 2011
    ..These data suggest that ECAT15-2 has important roles in lung development, where it is no longer expressed, by leaving epigenetic marks from earlier developmental stages...
  36. pmc Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A
    Masataka Fujiwara
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e16734. 2011
    ..These results provide a technological basis to obtain functional cardiomyocytes from iPSCs...
  37. pmc Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts
    Koji Tanabe
    Department of Reprogramming Science, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 110:12172-9. 2013
    ..These data demonstrate that maturation, and not initiation, is the limiting step during the direct reprogramming of human fibroblasts toward pluripotency and that each proreprogramming factor has a different mode of action...
  38. doi request reprint Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells
    Genta Narazaki
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Circulation 118:498-506. 2008
    ..However, detailed differentiation properties and the directional differentiation system of iPS cells have not been demonstrated...
  39. doi request reprint Transplantation of mouse induced pluripotent stem cells into the cochlea
    Koji Nishimura
    Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Neuroreport 20:1250-4. 2009
    ..Some transplants expressed vesicular glutamate transporter 1, which is a marker for glutamatergic neurons. These findings indicate that iPS cells can be used as a source of transplants for the regeneration of spiral ganglion neurons...
  40. doi request reprint Drug screening for ALS using patient-specific induced pluripotent stem cells
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 4:145ra104. 2012
    ..These findings suggest that motor neurons generated from ALS patient-derived iPSCs may provide a useful tool for elucidating ALS disease pathogenesis and for screening drug candidates...
  41. ncbi request reprint Intracellular signaling pathways regulating pluripotency of embryonic stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Curr Stem Cell Res Ther 1:103-11. 2006
    ..We will review the rapid progress that has been made in identifying and dissecting the intracellular signaling pathways that contribute to self-renewal of pluripotent mouse and human ES cells...
  42. pmc Pluripotency and nuclear reprogramming
    Shinya Yamanaka
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Philos Trans R Soc Lond B Biol Sci 363:2079-87. 2008
    ..Here, we review the molecular mechanisms underlying pluripotency and the currently known methods of inducing pluripotency in somatic cells...
  43. ncbi request reprint [Molecular mechanisms underlying pluripotency of embryonic stem cells]
    Shinya Yamanaka
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Seikagaku 78:27-33. 2006
  44. pmc Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)
    Hisayuki Amano
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    BMC Dev Biol 6:11. 2006
    ..Previous studies identified genomic clones containing the mouse ESG1 gene and five pseudogenes. However, their chromosomal localizations or physiological functions have not been determined...
  45. doi request reprint Induced pluripotent stem cells in medicine and biology
    Kazutoshi Takahashi
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, 606 8507, Japan
    Development 140:2457-61. 2013
    ..The ability to induce cell fate conversion is attractive not only for these applications, but also for basic research fields, such as development, cancer, epigenetics and aging...
  46. pmc Epigenetic regulation in pluripotent stem cells: a key to breaking the epigenetic barrier
    Akira Watanabe
    Center for iPS Cell Research and Application, Kyoto University
    Philos Trans R Soc Lond B Biol Sci 368:20120292. 2013
    ....
  47. pmc Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells
    Masatoshi Kajiwara
    Center for Induced Pluripotent Stem Cell Research and Application, Department of Surgery, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 109:12538-43. 2012
    ..These data underscore the importance of donor differences when comparing the differentiation propensities of hiPSC clones...
  48. pmc Human induced pluripotent stem cells on autologous feeders
    Kazutoshi Takahashi
    Center for iPS Cell Research and Application, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 4:e8067. 2009
    ..Instead of animal feeders, human fibroblasts can be used as feeder cells of human ES cells. However, one still has to be concerned about the existence of unidentified pathogens, such as viruses and prions in these non-autologous feeders...
  49. ncbi request reprint Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University and CREST, Japan Science and Technology Agency, Kyoto 606 8507, Japan
    J Biol Chem 280:32768-74. 2005
    ..Rheb also shares the same membrane-targeting pathway but because of the absence of palmitoylation is located on endomembranes...
  50. doi request reprint Generation and characterization of induced pluripotent stem cells from aid-deficient mice
    Ren Shimamoto
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    PLoS ONE 9:e94735. 2014
    ..A comprehensive DNA methylation analysis showed only a few differences between Aid+/+ and Aid-/- iPS cells. These data suggest that Aid does not have crucial functions in DNA demethylation during iPS cell generation. ..
  51. ncbi request reprint Ultrastructural maturation of human-induced pluripotent stem cell-derived cardiomyocytes in a long-term culture
    Tsukasa Kamakura
    Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Circ J 77:1307-14. 2013
    ..However, the maturation process in a long-term culture remains unknown...
  52. doi request reprint iPS cells: a game changer for future medicine
    Haruhisa Inoue
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    EMBO J 33:409-17. 2014
    ..The use of iPSCs in disease modeling, cell transplantation, and clinical trials could therefore lead to significant changes in the future of medicine. ..
  53. doi request reprint Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation
    Kotaro Ohnishi
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan Department of Medicine, Gifu University Graduate School of Medicine, Gifu 501 1194, Japan
    Cell 156:663-77. 2014
    ..These findings suggest that epigenetic regulation associated with iPSC derivation may drive development of particular types of cancer...
  54. doi request reprint Making steady progress on direct cardiac reprogramming toward clinical application
    Kenji Miki
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Circ Res 113:13-5. 2013
    ..A new report demonstrates direct cardiac reprogramming in human cells for the first time and points to the possibility of moving this technology toward clinical applications...
  55. ncbi request reprint [Induction of pluripotent stem cells from mouse fibroblast cultures]
    Shinya Yamanaka
    Tanpakushitsu Kakusan Koso 51:2346-51. 2006
  56. doi request reprint Perspectives for induced pluripotent stem cell technology: new insights into human physiology involved in somatic mosaicism
    Naoki Nagata
    From the Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan N N, S Y and Gladstone Institute of Cardiovascular Disease, San Francisco, CA S Y
    Circ Res 114:505-10. 2014
    ..In particular, we focus on the cloning event occurring through the reprogramming process and its ability to let us analyze the development of complex disease-harboring somatic mosaicism. ..
  57. ncbi request reprint Reprogramming somatic cells towards pluripotency by defined factors
    Marc Lewitzky
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Curr Opin Biotechnol 18:467-73. 2007
    ..The re-programmed cells contribute to live chimeric mice and are transmitted via the germline...
  58. pmc A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
    Masato Nakagawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Rep 4:3594. 2014
    ..These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system. ..
  59. doi request reprint Rapid and deep profiling of human induced pluripotent stem cell proteome by one-shot NanoLC-MS/MS analysis with meter-scale monolithic silica columns
    Ryota Yamana
    Department of Molecular and Cellular BioAnalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Proteome Res 12:214-21. 2013
    ..Our results indicate that this simplified one-shot proteomics approach with long monolithic columns is advantageous for rapid, deep, sensitive, and reproducible proteome analysis...
  60. pmc Induced pluripotent stem cells: opportunities and challenges
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Philos Trans R Soc Lond B Biol Sci 366:2198-207. 2011
    ..This report will summarize the current knowledge of iPS generation and discuss future reprogramming methods for medical application...
  61. pmc Nuclear reprogramming to a pluripotent state by three approaches
    Shinya Yamanaka
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Nature 465:704-12. 2010
    ..This remarkable discovery of cellular plasticity has important medical applications...
  62. pmc Anti-Aβ drug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer's disease
    Naoki Yahata
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e25788. 2011
    ..Induced pluripotent stem (iPS) cells potentially provide an opportunity to generate a human cell-based model of AD that would be crucial for drug discovery as well as for investigating mechanisms of the disease...
  63. doi request reprint iPS cells: a source of cardiac regeneration
    Yoshinori Yoshida
    Center for iPS Cell Research and Application, Kyoto University, Sakyo ku, Kyoto, Japan
    J Mol Cell Cardiol 50:327-32. 2011
    ..This article is part of a special issue entitled, "Cardiovascular Stem Cells Revisited"...
  64. doi request reprint Strategies and new developments in the generation of patient-specific pluripotent stem cells
    Shinya Yamanaka
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 1:39-49. 2007
    ..Taken together, these studies underscore the crucial roles of transcription factors and chromatin remodeling in nuclear reprogramming...
  65. doi request reprint A fresh look at iPS cells
    Shinya Yamanaka
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell 137:13-7. 2009
    ..The potential of induced pluripotent stem (iPS) cells is enormous, but many obstacles remain before their medical and pharmaceutical applications can be fully realized...
  66. doi request reprint Efficient and rapid induction of human iPSCs/ESCs into nephrogenic intermediate mesoderm using small molecule-based differentiation methods
    Toshikazu Araoka
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    PLoS ONE 9:e84881. 2014
    ..Such methods are powerful tools for studying kidney development and may potentially provide cell sources to generate renal lineage cells for regenerative therapy. ..
  67. doi request reprint Global splicing pattern reversion during somatic cell reprogramming
    Sho Ohta
    Department of Reprogramming Science, Center for iPS Cell Research and Application, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Cell Rep 5:357-66. 2013
    ..Our results indicate that the drastic alteration in splicing represents part of the molecular network involved in the reprogramming process. ..
  68. doi request reprint Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery
    Takayuki Tanaka
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Blood 120:1299-308. 2012
    ..Our results illustrate that patient-derived iPSCs are useful for dissecting somatic mosaicism and that NLRP3-mutant iPSCs can provide a valuable platform for drug discovery for multiple NLRP3-related disorders...
  69. ncbi request reprint [Research for cell therapy by induced pluripotent stem cell]
    Hidetoshi Sakurai
    Center for iPS Cell Research and Application CiRA, Kyoto University
    Nihon Rinsho 69:2114-8. 2011
    ..This review is also focused on new strategies for reducing a risk of tumorigenesis...
  70. doi request reprint Induction of pluripotency by defined factors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Exp Cell Res 316:2565-70. 2010
    ..On the other hand, patient-specific iPS cells have already been established and used for recapitulating disease pathology...
  71. doi request reprint Reprogramming of somatic cells to pluripotency
    Masato Nakagawa
    Center for iPS Research and Application Institute for Integrated Cell Material Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, 606 8507, Japan
    Adv Exp Med Biol 695:215-24. 2010
    ....