S Tsukita

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins
    S Tsukita
    College of Medical Technology, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 274:34507-10. 1999
  2. ncbi request reprint ERM proteins: head-to-tail regulation of actin-plasma membrane interaction
    S Tsukita
    College of Medical Technology, Kyoto University, Japan
    Trends Biochem Sci 22:53-8. 1997
  3. pmc Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins
    M Itoh
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 147:1351-63. 1999
  4. pmc Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2
    S Yonemura
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 01, Japan
    J Cell Biol 140:885-95. 1998
  5. pmc Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins
    S Yonemura
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 145:1497-509. 1999
  6. pmc Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions
    M Itoh
    1Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 154:491-7. 2001
  7. ncbi request reprint Transglutaminase type 1 and its cross-linking activity are concentrated at adherens junctions in simple epithelial cells
    T Hiiragi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Biol Chem 274:34148-54. 1999
  8. pmc Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Proc Natl Acad Sci U S A 96:511-6. 1999
  9. ncbi request reprint Mechanism of glia-neuron cell-fate switch in the Drosophila thoracic neuroblast 6-4 lineage
    Y Akiyama-Oda
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Development 127:3513-22. 2000
  10. ncbi request reprint Subcellular distribution of tight junction-associated proteins (occludin, ZO-1, ZO-2) in rodent skin
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Invest Dermatol 110:862-6. 1998

Collaborators

Detail Information

Publications72

  1. ncbi request reprint Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins
    S Tsukita
    College of Medical Technology, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 274:34507-10. 1999
  2. ncbi request reprint ERM proteins: head-to-tail regulation of actin-plasma membrane interaction
    S Tsukita
    College of Medical Technology, Kyoto University, Japan
    Trends Biochem Sci 22:53-8. 1997
    ..Specific signals activate ERM proteins to bind actin filaments and the plasma membrane; these include phosphoinositides and/or phosphorylation mechanisms, which might be located downstream from the Rho-dependent pathway...
  3. pmc Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins
    M Itoh
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 147:1351-63. 1999
    ..In good agreement, PDZ1 and PDZ2 domains of ZO-1/ZO-2/ZO-3 were also recruited to claudin-based TJs, when introduced into cultured epithelial cells. The possible molecular architecture of TJ plaque structures is discussed...
  4. pmc Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2
    S Yonemura
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 01, Japan
    J Cell Biol 140:885-95. 1998
    ..From these findings, we conclude that ERM proteins bind to integral membrane proteins bearing a positively charged amino acid cluster in their juxta-membrane cytoplasmic domain...
  5. pmc Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins
    S Yonemura
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 145:1497-509. 1999
    ..Furthermore, immunodetection with a phosphorylated ERM-specific antibody and site-directed mutagenesis suggested that ERM proteins phosphorylated at their COOH-terminal threonine residue represent activated ERM proteins...
  6. pmc Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions
    M Itoh
    1Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 154:491-7. 2001
    ..We also discuss the importance of this model from the perspective of the general molecular mechanisms behind the recruitment of PAR proteins to plasma membranes...
  7. ncbi request reprint Transglutaminase type 1 and its cross-linking activity are concentrated at adherens junctions in simple epithelial cells
    T Hiiragi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Biol Chem 274:34148-54. 1999
    ....
  8. pmc Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Proc Natl Acad Sci U S A 96:511-6. 1999
    ..These findings indicated that multiple claudin family members are involved in the formation of TJ strands in various tissues...
  9. ncbi request reprint Mechanism of glia-neuron cell-fate switch in the Drosophila thoracic neuroblast 6-4 lineage
    Y Akiyama-Oda
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Development 127:3513-22. 2000
    ..Furthermore, we showed that outside the NB6-4T lineage most glial cells appeared independently of Pros...
  10. ncbi request reprint Subcellular distribution of tight junction-associated proteins (occludin, ZO-1, ZO-2) in rodent skin
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Invest Dermatol 110:862-6. 1998
    ..In addition, ZO-1 but not occludin were localized weakly at the outer root sheath and intensely at the hair cortex/matrix...
  11. pmc Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 147:185-94. 1999
    ..e., the extracellular face-associated TJs. These findings indicated that claudin-5/TMVCF is an endothelial cell-specific component of TJ strands...
  12. ncbi request reprint Multifunctional strands in tight junctions
    S Tsukita
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Nat Rev Mol Cell Biol 2:285-93. 2001
    ..New insights into the molecular architecture of tight junctions allow us to now discuss the structure and functions of this unique cell-cell adhesion apparatus in molecular terms...
  13. ncbi request reprint Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin
    M Itoh
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 274:5981-6. 1999
    ..These findings indicated that ZO-2 forms a complex with ZO-1/occludin or ZO-1/alpha catenin to establish TJ or AJ domains, respectively...
  14. pmc Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
    T Matsui
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 140:647-57. 1998
    ....
  15. pmc Manner of interaction of heterogeneous claudin species within and between tight junction strands
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 147:891-903. 1999
    ..We concluded that distinct species of claudins can interact within and between TJ strands, except in some combinations. This mode of assembly of claudins could increase the diversity of the structure and functions of TJ strands...
  16. ncbi request reprint Normal development of mice and unimpaired cell adhesion/cell motility/actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout
    Y Doi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 274:2315-21. 1999
    ..These findings favor the notion that ERM proteins are functionally redundant at the cellular as well as the whole body level...
  17. pmc Claudin-11/OSP-based tight junctions of myelin sheaths in brain and Sertoli cells in testis
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 145:579-88. 1999
    ....
  18. pmc A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 143:391-401. 1998
    ..These findings suggested that claudin-1 and -2 are mainly responsible for TJ strand formation, and that occludin is an accessory protein in some function of TJ strands...
  19. pmc Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes
    Y Nakagawa
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
    Mol Biol Cell 12:1687-97. 2001
    ..These findings indicate that Odf2 is a widespread KI-insoluble scaffold component of the centrosome matrix, which may be involved in the maturation event of daughter centrioles...
  20. ncbi request reprint Occludin is concentrated at tight junctions of mouse/rat but not human/guinea pig Sertoli cells in testes
    S Moroi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Am J Physiol 274:C1708-17. 1998
    ....
  21. pmc ERM (ezrin/radixin/moesin)-based molecular mechanism of microvillar breakdown at an early stage of apoptosis
    T Kondo
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 139:749-58. 1997
    ..The physiological relevance of this ERM protein-based microvillar breakdown in apoptosis will be discussed...
  22. pmc Complex phenotype of mice lacking occludin, a component of tight junction strands
    M Saitou
    Department of Cell Biology, Kyoto University Faculty of Medicine, Sakyo ku, Kyoto 606 8501, Japan
    Mol Biol Cell 11:4131-42. 2000
    ..These phenotypes suggested that the functions of TJs as well as occludin are more complex than previously supposed...
  23. pmc Functional domains of alpha-catenin required for the strong state of cadherin-based cell adhesion
    Y Imamura
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 144:1311-22. 1999
    ..Our studies showed that the interaction of alpha-catenin with the actin-based cytoskeleton through the ZO-1-binding domain is required for the strong state of E-cadherin-based cell adhesion activity...
  24. pmc Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 153:263-72. 2001
    ....
  25. pmc Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions
    M Saitou
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 141:397-408. 1998
    ..These findings indicate that there are as yet unidentified TJ integral membrane protein(s) which can form strand structures, recruit ZO-1, and function as a barrier without occludin...
  26. pmc Possible involvement of phosphorylation of occludin in tight junction formation
    A Sakakibara
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 137:1393-401. 1997
    ..These findings suggest that the phosphorylation of occludin is a key step in tight junction assembly...
  27. ncbi request reprint Mammalian occludin in epithelial cells: its expression and subcellular distribution
    M Saitou
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    Eur J Cell Biol 73:222-31. 1997
    ..Furthermore, the exclusive concentration of occludin at tight junctions in epithelial cells was confirmed by immunoreplica electron microscopy...
  28. ncbi request reprint Dynamics of connexins, E-cadherin and alpha-catenin on cell membranes during gap junction formation
    K Fujimoto
    Department of Anatomy, Faculty of Medicine, Kyoto University, Japan
    J Cell Sci 110:311-22. 1997
    ..In addition, connexin-immunoreactivity was also observed along tight junctional strands, suggesting that the gap junction may also form along the tight junctions...
  29. pmc Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments
    M Itoh
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606, Japan
    J Cell Biol 138:181-92. 1997
    ....
  30. pmc Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 141:1539-50. 1998
    ....
  31. pmc Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway
    M Hirao
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Cell Biol 135:37-51. 1996
    ..These findings indicate that Rho regulates the CD44/ERM complex formation in vivo and that the phosphatidylinositol turnover may be involved in this regulation mechanism...
  32. ncbi request reprint The structure and function of claudins, cell adhesion molecules at tight junctions
    S Tsukita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Ann N Y Acad Sci 915:129-35. 2000
    ..Now that claudins have been identified, the structure and functions of TJs should be determined in detail in molecular terms...
  33. ncbi request reprint ERM (ezrin/radixin/moesin) family: from cytoskeleton to signal transduction
    S Tsukita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606, Japan
    Curr Opin Cell Biol 9:70-5. 1997
    ..Furthermore, identification of the neurofibromatosis type 2 tumour suppressor, which shows striking sequence similarity to ERM proteins, has increased interest in this family...
  34. ncbi request reprint Structural conversion between open and closed forms of radixin: low-angle shadowing electron microscopy
    H Ishikawa
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Mol Biol 310:973-8. 2001
    ..Taken together with the biochemical and the crystal structural results obtained to date, the closed and open forms represent the inactive and active forms of radixin as cross-linkers between actin filaments and plasma membranes...
  35. ncbi request reprint Occludin and claudins in tight-junction strands: leading or supporting players?
    S Tsukita
    Dept of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Trends Cell Biol 9:268-73. 1999
    ..This review discusses current understanding of the molecular architecture of tight-junction strands, focusing on the recent discovery of two distinct types of tight-junction-specific integral membrane proteins, occludin and claudins...
  36. ncbi request reprint Tight junction-associated proteins (occludin, ZO-1, claudin-1, claudin-4) in squamous cell carcinoma and Bowen's disease
    K Morita
    Department of Dermatology, Graduate School of Medicine, Kyoto University, Shogoinkawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Br J Dermatol 151:328-34. 2004
    ..The epidermis, which is a typical stratified epithelium, has tight junctions (TJs) in the granular layer, as do simple epithelia. So far, abnormalities of TJs and involvement of claudin-1 have been reported in tumours of simple epithelia...
  37. ncbi request reprint Overcoming barriers in the study of tight junction functions: from occludin to claudin
    S Tsukita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Genes Cells 3:569-73. 1998
    ..Here, we present an overview of current progress in the identification of TJ-specific integral membrane proteins...
  38. pmc Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues
    Y Ando-Akatsuka
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Cell Biol 133:43-7. 1996
    ..Implications of these data and novel experimental options in cell biological research are discussed...
  39. pmc Pores in the wall: claudins constitute tight junction strands containing aqueous pores
    S Tsukita
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501 Japan
    J Cell Biol 149:13-6. 2000
  40. ncbi request reprint Distinct mechanisms triggering glial differentiation in Drosophila thoracic and abdominal neuroblasts 6-4
    Y Akiyama-Oda
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto, 606 8501, Japan
    Dev Biol 222:429-39. 2000
    ..These results showed that NB6-4T, but not NB6-4A, requires cell cycle progression for acquisition of glial fate, suggesting that distinct mechanisms trigger glial differentiation in the different lineages...
  41. pmc Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein
    H Oda
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    J Cell Biol 121:1133-40. 1993
    ..These results strongly suggest that Drosophila has a cell adhesion machinery homologous to the vertebrate cadherin-catenin system...
  42. ncbi request reprint Involvement of claudin-1 in the beta-catenin/Tcf signaling pathway and its frequent upregulation in human colorectal cancers
    N Miwa
    Laboratory of Molecular Medicine, Institute of Medical Science, The University of Tokyo, Japan
    Oncol Res 12:469-76. 2001
    ..Our results imply that claudin-1 is involved in the beta-catenin-Tcf/LEF signaling pathway, and that increased expression of claudin-1 may have some role in colorectal tumorigenesis...
  43. ncbi request reprint The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules
    Y Mimori-Kiyosue
    Tsukita Cell Axis Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Shimogyo ku, 600 8813, Japan
    Curr Biol 10:865-8. 2000
    ..These findings showed that the APC-EB1 interaction is regulated within cells and is allowed near the ends of microtubules only under restricted conditions...
  44. ncbi request reprint Dynamic changes in protein components of the tight junction during liver regeneration
    Y Takaki
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, Japan
    Cell Tissue Res 305:399-409. 2001
    ....
  45. ncbi request reprint Crystallization and preliminary crystallographic studies of RhoGDI in complex with the radixin FERM domain
    K Hamada
    Department of Molecular Biology, Nara Institute of Science and Technology (NAIST, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
    Acta Crystallogr D Biol Crystallogr 57:889-90. 2001
    ..9 (2), b = 151.2 (2), c = 71.2 (1) A, and contain two protein complexes in the crystallographic asymmetric unit. A 2.9 A resolution data set was collected using synchrotron radiation at SPring-8...
  46. ncbi request reprint Nonchordate classic cadherins have a structurally and functionally unique domain that is absent from chordate classic cadherins
    H Oda
    Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Kyoto Research Park, Chudoji Minami machi, Shimogyo ku, Kyoto, 600 8813, Japan
    Dev Biol 216:406-22. 1999
    ..We propose that there is a fundamental difference in the mode of classic cadherin-mediated cell-cell adhesion between chordate and nonchordate metazoans...
  47. ncbi request reprint Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of the adhesion protein ICAM-2
    K Hamada
    Department of Molecular Biology, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    Acta Crystallogr D Biol Crystallogr 57:891-2. 2001
    ..44 (9), c = 99.49 (6) A, and contain one complex in the crystallographic asymmetric unit. An intensity data set was collected to a resolution of 2.60 A...
  48. ncbi request reprint Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions
    K Kubota
    Department of Cell Biology Faculty of Medicine Kyoto University Sakyo ku, Kyoto, 606 8501, Japan
    Curr Biol 9:1035-8. 1999
    ..Thus, claudins are responsible for TJ-specific obliteration of the intercellular space...
  49. ncbi request reprint The 102 kd cadherin-associated protein: similarity to vinculin and posttranscriptional regulation of expression
    A Nagafuchi
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Cell 65:849-57. 1991
    ..The same effect was observed by introducing N- or P-cadherin into L cells...
  50. pmc Radixin is a novel member of the band 4.1 family
    N Funayama
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    J Cell Biol 115:1039-48. 1991
    ..1 protein described in this study favors the idea that radixin plays a crucial role in the association of the barbed ends of actin filaments with the plasma membrane in the cell-to-cell adherens junction and the cleavage furrow...
  51. pmc Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    J Cell Biol 127:1617-26. 1994
    ..The coincidence of the sequence necessary for the ZO-1 association with that for the TJ localization suggests that the association with underlying cytoskeletons through ZO-1 is required for occludin to be localized at TJ...
  52. pmc The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules
    A Nagafuchi
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    J Cell Biol 127:235-45. 1994
    ..The possible functions of beta catenin are discussed with a special reference to its role as a negative regulator for the cadherin-mediated cell adhesion system...
  53. ncbi request reprint A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular adhesiveness in human cancer cell lines
    T Oyama
    Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer Res 54:6282-7. 1994
    ..These findings indicate that genetic abnormality of beta-catenin is one of the mechanisms responsible for loosening of cell-cell contact, and may be involved in enhancement of tumor invasion in human cancers...
  54. ncbi request reprint Structural diversity of band 4.1 superfamily members
    K Takeuchi
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Sci 107:1921-8. 1994
    ..In this study, we describe the initial characterization of these new members and discuss the evolution of the band 4.1 superfamily...
  55. ncbi request reprint Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK
    T Hashimoto
    Department of Dermatology, Keio University School of Medicine, Shinjuku, Tokyo
    J Cell Sci 105:275-86. 1993
    ..These findings suggest that the desmoyokin/AHNAK protein is a ubiquitous molecule with a unique structure and appears to have different distributions (and probably different functions) among different cells...
  56. pmc The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy
    M Itoh
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 121:491-502. 1993
    ..Possible functions of the 220-kD protein (ZO-1) are discussed with special reference to the molecular mechanism for adherens and tight junction formation...
  57. ncbi request reprint Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein
    K Takahashi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
    J Biol Chem 272:23371-5. 1997
    ..These results suggest that ERM as well as Rho GDI and the Rho GDP/GTP exchange factor are involved in the activation of the Rho subfamily members, which then regulate reorganization of actin filaments through the ERM system...
  58. ncbi request reprint Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein
    H Oda
    Tsukita Cell Axis Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Kyoto Research Park, Chudoji Minami machi, Shimogyo ku, Kyoto 600 8813, Japan
    Dev Genes Evol 209:218-25. 1999
    ..Thus, the Dalpha-catenin-GFP fusion protein is a helpful tool to simultaneously observe morphogenetic movements and AJ dynamics at high spatio-temporal resolution...
  59. pmc Expression of occludin, tight-junction-associated protein, in human digestive tract
    Y Kimura
    Department of Surgery II, Osaka University Medical School, Japan
    Am J Pathol 151:45-54. 1997
    ..In addition, occludin expression can serve as a histopathological indicator for differentiation in gastrointestinal adenocarcinomas...
  60. ncbi request reprint Occludin as a possible determinant of tight junction permeability in endothelial cells
    T Hirase
    Eisai London Research Laboratories Ltd, University College London, UK
    J Cell Sci 110:1603-13. 1997
    ..Our data indicate that regulation of occludin expression may be a crucial determinant of the tight junction permeability properties of endothelial cells in different tissues...
  61. ncbi request reprint A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites
    N Sato
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Sci 103:131-43. 1992
    ..We conclude that at least one of the members of the ezrin-radixin-moesin family is concentrated at specific regions where actin filaments are densely associated with plasma membranes...
  62. ncbi request reprint Dynamic behavior of the cadherin-based cell-cell adhesion system during Drosophila gastrulation
    H Oda
    Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Kyoto Research Park, Kyoto, Chudoji Minami machi, Shimogyo ku, 600 8813, Japan
    Dev Biol 203:435-50. 1998
    ..Moreover, the processes responsible for loss of epithelial cell polarity and elimination of preexisting DE-cadherin-based epithelial junctions during early mesodermal morphogenesis are discussed...
  63. pmc Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
    K Mandai
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
    J Cell Biol 139:517-28. 1997
    ..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
  64. ncbi request reprint Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein
    V V Verkhusha
    Tsukita Cell Axis Project, ERATO, JST, Kyoto, Japan
    FEBS Lett 445:395-401. 1999
    ..Our results favored a treadmilling model for actin behavior in border cell lamellipodia...
  65. pmc Where is APC going?
    Y Mimori-Kiyosue
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    J Cell Biol 154:1105-9. 2001
    ....
  66. ncbi request reprint A 155-kDa undercoat-constitutive protein of cell-to-cell adherens junctions
    T Hirase
    Department of Cell Biology, Kyoto University Faculty of Medicine, Kyoto Japan
    Eur J Cell Biol 72:174-81. 1997
    ..We conclude that E14 is a novel undercoat-constitutive protein found in certain types of cell-to-cell adherens junctions...
  67. pmc Autoimmune myocarditis induced in mice by cardiac C-protein. Cloning of complementary DNA encoding murine cardiac C-protein and partial characterization of the antigenic peptides
    H Kasahara
    First Department of Internal Medicine, Nagoya University School of Medicine, Japan
    J Clin Invest 94:1026-36. 1994
    ..This study provides a new animal model of autoimmune myocarditis which may shed light on the pathogenesis of dilated cardiomyopathy...
  68. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
    ..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
  69. ncbi request reprint Structure, expression and chromosome assignment of the human catenin (cadherin-associated protein) alpha 1 gene (CTNNA1)
    Y Furukawa
    Department of Biochemistry, Cancer Institute, Tokyo, Japan
    Cytogenet Cell Genet 65:74-8. 1994
    ..2% homologous. Analysis by reverse transcription-PCR revealed that this gene is expressed ubiquitously in normal tissues. It was mapped to chromosome band 5q31 by fluorescent in situ hybridization...
  70. ncbi request reprint Real-time imaging of cell-cell adherens junctions reveals that Drosophila mesoderm invagination begins with two phases of apical constriction of cells
    H Oda
    Tsukita Cell Axis Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Kyoto Research Park, Chudoji Minami machi, Shimogyo ku, Kyoto 600 8813, Japan
    J Cell Sci 114:493-501. 2001
    ..Movies available on-line: http://www.biologists.com/JCS/movies/jcs2073.html..
  71. ncbi request reprint Cloning of the human alpha-catenin cDNA and its aberrant mRNA in a human cancer cell line
    T Oda
    Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
    Biochem Biophys Res Commun 193:897-904. 1993
    ..These deletions were probably produced by an error of RNA splicing, presenting one possible mechanism for the loss of intact alpha-catenin expression...
  72. ncbi request reprint Epithelial transport and barrier function in occludin-deficient mice
    J D Schulzke
    Department of Gastroenterology, Campus Benjamin Franklin, Charité Universitary Medicine Berlin, Germany
    Biochim Biophys Acta 1669:34-42. 2005
    ..This study aimed at functional characterization of the tight junction protein occludin using the occludin-deficient mouse model...