Makoto Tachibana

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. pmc Functional dynamics of H3K9 methylation during meiotic prophase progression
    Makoto Tachibana
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 26:3346-59. 2007
  2. pmc G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription
    Makoto Tachibana
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 27:2681-90. 2008
  3. pmc Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9
    Makoto Tachibana
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    Genes Dev 19:815-26. 2005
  4. pmc Histone H1 null vertebrate cells exhibit altered nucleosome architecture
    Hideharu Hashimoto
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, Japan
    Nucleic Acids Res 38:3533-45. 2010
  5. doi request reprint A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9
    Haruki Shirato
    Mitsubishi Kagaku Institute of Life Sciences, Kyoto, Japan
    J Biol Chem 284:733-9. 2009
  6. ncbi request reprint Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP
    Jun Ueda
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Japan
    J Biol Chem 281:20120-8. 2006
  7. doi request reprint Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET
    Toshiyuki Matsui
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin, Kawara cho, Sakyo ku, Kyoto 606 8507, Japan
    Nature 464:927-31. 2010
  8. pmc G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis
    Makoto Tachibana
    Department of Cell Biology, Institute for Virus Research, Kyoto University, Shogoin Kawara cho, Kyoto 606 8507, Japan
    Genes Dev 16:1779-91. 2002
  9. pmc Pericentric heterochromatin generated by HP1 protein interaction-defective histone methyltransferase Suv39h1
    Daisuke Muramatsu
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    J Biol Chem 288:25285-96. 2013
  10. pmc Distinct roles of TRF1 in the regulation of telomere structure and lengthening
    Keiji Okamoto
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, and Graduate School of Biostudies, Kyoto University, 53 Shogoin, Kawara cho, Sakyo ku, Kyoto, Japan
    J Biol Chem 283:23981-8. 2008

Collaborators

Detail Information

Publications32

  1. pmc Functional dynamics of H3K9 methylation during meiotic prophase progression
    Makoto Tachibana
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 26:3346-59. 2007
    ..This genetic and biochemical evidence strongly suggests that a specific set of H3K9 methyltransferase(s) and demethylase(s) coordinately regulate gametogenesis...
  2. pmc G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription
    Makoto Tachibana
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 27:2681-90. 2008
    ..This is the first clear evidence that G9a/GLP suppresses transcription by independently inducing both H3K9 and DNA methylation...
  3. pmc Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9
    Makoto Tachibana
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    Genes Dev 19:815-26. 2005
    ..Taken together, our new findings revealed that G9a and GLP cooperatively exert H3-K9 methyltransferase function in vivo, likely through the formation of higher-order heteromeric complexes...
  4. pmc Histone H1 null vertebrate cells exhibit altered nucleosome architecture
    Hideharu Hashimoto
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, Japan
    Nucleic Acids Res 38:3533-45. 2010
    ....
  5. doi request reprint A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9
    Haruki Shirato
    Mitsubishi Kagaku Institute of Life Sciences, Kyoto, Japan
    J Biol Chem 284:733-9. 2009
    ..These results suggest that Jmj methylates H3-K9 and represses cyclin D1 expression through G9a and GLP, and that Jmj family proteins can regulate gene expression by not only histone demethylation but also other histone modification...
  6. ncbi request reprint Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP
    Jun Ueda
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Japan
    J Biol Chem 281:20120-8. 2006
    ..These data indicate that Wiz not only contributes to the stability of G9a but also links the G9a/GLP heteromeric complex to the CtBP co-repressor machinery...
  7. doi request reprint Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET
    Toshiyuki Matsui
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin, Kawara cho, Sakyo ku, Kyoto 606 8507, Japan
    Nature 464:927-31. 2010
    ..We propose that a DNA-methylation-independent pathway involving KAP1 and ESET/ESET-mediated H3K9me3 is required for proviral silencing during the period early in embryogenesis when DNA methylation is dynamically reprogrammed...
  8. pmc G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis
    Makoto Tachibana
    Department of Cell Biology, Institute for Virus Research, Kyoto University, Shogoin Kawara cho, Kyoto 606 8507, Japan
    Genes Dev 16:1779-91. 2002
    ..Our results indicate that euchromatic H3-K9 methylation regulated by G9a is essential for early embryogenesis and is involved in the transcriptional repression of developmental genes...
  9. pmc Pericentric heterochromatin generated by HP1 protein interaction-defective histone methyltransferase Suv39h1
    Daisuke Muramatsu
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    J Biol Chem 288:25285-96. 2013
    ..These findings clearly showed that the Suv39h-HP1 binding is dispensable for pericentric H3K9me3 and DNA methylation, but this interaction and HP1 recruitment/accumulation seem to be crucial for complete formation of heterochromatin. ..
  10. pmc Distinct roles of TRF1 in the regulation of telomere structure and lengthening
    Keiji Okamoto
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, and Graduate School of Biostudies, Kyoto University, 53 Shogoin, Kawara cho, Sakyo ku, Kyoto, Japan
    J Biol Chem 283:23981-8. 2008
    ....
  11. doi request reprint Characterization of Drosophila G9a in vivo and identification of genetic interactants
    Yasuko Kato
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Genes Cells 13:703-22. 2008
    ..Furthermore co-expression of Lid in eye imaginal discs enhanced the rough phenotype induced by dG9a. The results suggest that the function of dG9a is negatively regulated by the PcG complex and positively regulated by Lid in vivo...
  12. ncbi request reprint Histone H1 variant, H1R is involved in DNA damage response
    Hideharu Hashimoto
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    DNA Repair (Amst) 6:1584-95. 2007
    ..Together, these findings provide the first genetic evidence that a specific H1 variant plays a unique and important role in the DNA damage response in vertebrates...
  13. ncbi request reprint Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a
    Miki Nishida
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Genes Cells 12:877-88. 2007
    ..Furthermore, ZNF200 appear to co-localize with G9a in the nucleoplasm of HEK293 cells as discrete speckles. These results demonstrate that ZNF200 is a novel binding partner of G9a...
  14. doi request reprint Epigenetic regulation of mouse sex determination by the histone demethylase Jmjd1a
    Shunsuke Kuroki
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin, Kawara cho, Sakyo ku, Kyoto, Japan
    Science 341:1106-9. 2013
    ..Jmjd1a directly and positively controls Sry expression by regulating H3K9me2 marks. These studies reveal a pivotal role of histone demethylation in mammalian sex determination. ..
  15. doi request reprint JMJD1C, a JmjC domain-containing protein, is required for long-term maintenance of male germ cells in mice
    Shunsuke Kuroki
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, Japan
    Biol Reprod 89:93. 2013
    ..Our studies demonstrated that JMJD1C contributes to the long-term maintenance of the male germ line. ..
  16. doi request reprint Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells
    Katsuaki Deguchi
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    Biol Reprod 88:36. 2013
    ....
  17. ncbi request reprint [Mammalian histone methyltransferases]
    Makoto Tachibana
    Experimental Research Center for Infection Diseases, Institute for Virus Research, Kyoto University, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Seikagaku 78:50-3. 2006
  18. doi request reprint Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a
    Kohei Yamamizu
    Department of Stem Cell Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 10:759-70. 2012
    ..5 and delayed development. In this study, we demonstrate molecular machinery that regulates timing of multilineage differentiation by linking signaling with epigenetics...
  19. ncbi request reprint Rad54 is dispensable for the ALT pathway
    Koichi Akiyama
    Department of Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    Genes Cells 11:1305-15. 2006
    ..This is the first genetic evidence that mouse Rad54 is dispensable for the ALT pathway...
  20. pmc H3K9 methyltransferase G9a and the related molecule GLP
    Yoichi Shinkai
    Institute for Virus Research, Kyoto University, Kawara cho, Sakyo ku, Kyoto, Kyoto 606 8507, Japan
    Genes Dev 25:781-8. 2011
    ..Recently, many important studies have reported that G9a and GLP play critical roles in various biological processes. The physiological relevance of G9a/GLP-mediated epigenetic gene regulation is discussed...
  21. ncbi request reprint Importance of TRF1 for functional telomere structure
    Tomohiko Iwano
    Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, Japan
    J Biol Chem 279:1442-8. 2004
    ..These results strongly suggest that TRF1 interacts with other telomere-binding molecules and integrates into the functional telomere structure...
  22. ncbi request reprint Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice
    Yoshiyuki Seki
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Development 134:2627-38. 2007
    ..We suggest the possibility that active repression of an essential enzyme and subsequent unique cellular dynamics ensures successful implementation of genome-wide epigenetic reprogramming in migrating PGCs...
  23. ncbi request reprint X-inactivation is stably maintained in mouse embryos deficient for histone methyl transferase G9a
    Tatsuya Ohhata
    PRESTO, Japan Science and Technology Agency JST, Kawaguchi, Japan
    Genesis 40:151-6. 2004
    ..These results demonstrate that G9a is not essential for X-inactivation...
  24. ncbi request reprint Role of histone methyltransferase G9a in CpG methylation of the Prader-Willi syndrome imprinting center
    Zhenghan Xin
    Departments of Biochemistry and Molecular Genetics and Pediatrics, University of Virginia, Charlottesville, Virginia 22908 0733, USA
    J Biol Chem 278:14996-5000. 2003
    ....
  25. ncbi request reprint Methyl-CpG binding domain 1 (MBD1) interacts with the Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression
    Naoyuki Fujita
    Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    J Biol Chem 278:24132-8. 2003
    ..These data indicate that MBD1 may tether the Suv39h1-HP1 complex to methylated DNA regions, suggesting the presence of a pathway from DNA methylation to the modifications of histones for epigenetic gene regulation...
  26. pmc DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity
    Kevin B Dong
    Department of Medical Genetics, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia, Canada
    EMBO J 27:2691-701. 2008
    ..Taken together, these observations reveal that H3K9me3 and HP1alpha recruitment to retrotransposons occurs independent of DNA methylation in ES cells and that G9a promotes DNA methylation independent of its HMTase activity...
  27. pmc Functional analysis of histone methyltransferase g9a in B and T lymphocytes
    Lance R Thomas
    Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    J Immunol 181:485-93. 2008
    ..These findings indicate that the H3K9me2 epigenetic mark affects a highly restricted set of processes during lymphocyte development and activation...
  28. ncbi request reprint Targeted inhibition of V(D)J recombination by a histone methyltransferase
    Oleg Osipovich
    Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    Nat Immunol 5:309-16. 2004
    ..These findings indicate a key function for histone methyltransferases in the tissue- and stage-specific suppression of antigen receptor gene assembly during lymphocyte development...
  29. ncbi request reprint Partitioning and plasticity of repressive histone methylation states in mammalian chromatin
    Antoine H F M Peters
    Research Institute of Molecular Pathology, The Vienna Biocenter, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Mol Cell 12:1577-89. 2003
    ..Our data underscore the selective presence of distinct histone lysine methylation states in partitioning chromosomal subdomains but also reveal a surprising plasticity in propagating methylation patterns in eukaryotic chromatin...
  30. ncbi request reprint Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells
    Kohta Ikegami
    Laboratory of Cellular Biochemistry, Department of Animal Resource Sciences and Veterinary Medical Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Genes Cells 12:1-11. 2007
    ..Chromatin immunoprecipitation (ChIP) confirmed these loci to be targets of G9a, with decreased H3-K9 and/or -K27 dimethylation in the G9a(-/-) cells. These data indicate that G9a site-selectively contributes to DNA methylation...
  31. pmc In vitro and in vivo analyses of a Phe/Tyr switch controlling product specificity of histone lysine methyltransferases
    Robert E Collins
    Biochemistry and Chemistry, Emory University, Atlanta, GA 30392, USA
    J Biol Chem 280:5563-70. 2005
    ..The altered DIM-5 rescued the growth defect characteristic of dim-5 N. crassa but did not fully rescue the gross DNA hypomethylation of dim-5 strains...
  32. ncbi request reprint Molecular dynamics of Aurora-A kinase in living mitotic cells simultaneously visualized with histone H3 and nuclear membrane protein importinalpha
    Kenji Sugimoto
    Laboratory of Applied Molecular Biology, Division of Applied Biochemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1 1 Gakuen cho, Sakai, Osaka 599 8531, Japan
    Cell Struct Funct 27:457-67. 2002
    ..Immunostaining with anti-alpha or gamma-tubulin further indicated that Aurora-A was involved in the formation of mitotic spindle in metaphase as well as the subsequent chromosome movement in anaphase...