Y Sasakura

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Ascidian Wnt-7 gene is expressed exclusively in the tail neural tube of tailbud embryos
    Y Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Genes Evol 210:641-3. 2000
  2. ncbi request reprint Application of Minos, one of the Tc1/mariner superfamily transposable elements, to ascidian embryos as a tool for insertional mutagenesis
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Gene 308:11-20. 2003
  3. ncbi request reprint Identification of cis elements which direct the localization of maternal mRNAs to the posterior pole of ascidian embryos
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Biol 250:128-44. 2002
  4. ncbi request reprint Ascidian Wnt-5 gene is involved in the morphogenetic movement of notochord cells
    Y Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 43:573-82. 2001
  5. ncbi request reprint A gene encoding a new ONECUT class homeodomain protein in the ascidian Halocynthia roretzi functions in the differentiation and specification of neural cells in ascidian embryogenesis
    Y Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
    Mech Dev 104:37-48. 2001
  6. ncbi request reprint Large-scale cDNA analysis of the maternal genetic information in the egg of Halocynthia roretzi for a gene expression catalog of ascidian development
    K W Makabe
    Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Development 128:2555-67. 2001
  7. ncbi request reprint Gene expression profiles in Ciona intestinalis tailbud embryos
    Y Satou
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
    Development 128:2893-904. 2001
  8. ncbi request reprint Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis
    T Kawashima
    Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Dev Genes Evol 210:162-5. 2000
  9. ncbi request reprint Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos
    Yasunori Sasakura
    Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan
    Dev Dyn 237:39-50. 2008
  10. pmc Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis
    Yasunori Sasakura
    Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415 0025, Japan
    Genome Biol 8:S3. 2007

Detail Information

Publications27

  1. ncbi request reprint Ascidian Wnt-7 gene is expressed exclusively in the tail neural tube of tailbud embryos
    Y Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Genes Evol 210:641-3. 2000
    ..We report here that a new member of the ascidian Wnt gene family, HrWnt-7, was expressed in the tail neural tube at the early tailbud stage. Moreover, in cross-section, HrWnt-7 was expressed in the dorsal and ventral ependymal cells...
  2. ncbi request reprint Application of Minos, one of the Tc1/mariner superfamily transposable elements, to ascidian embryos as a tool for insertional mutagenesis
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Gene 308:11-20. 2003
    ..From these tests, we concluded that Minos transposase has complete activity when it is expressed in C. intestinalis, suggesting that Minos has the potential to be used for genome-wide insertional mutagenesis of C. intestinalis...
  3. ncbi request reprint Identification of cis elements which direct the localization of maternal mRNAs to the posterior pole of ascidian embryos
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Biol 250:128-44. 2002
    ..These findings, especially the identification of the small localization element of HrWnt-5 mRNA, provide new insights into the localization of mRNAs during ascidian embryogenesis...
  4. ncbi request reprint Ascidian Wnt-5 gene is involved in the morphogenetic movement of notochord cells
    Y Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 43:573-82. 2001
    ..These results suggest that HrWnt-5 regulates the morphogenetic movement of notochord cells during ascidian embryogenesis...
  5. ncbi request reprint A gene encoding a new ONECUT class homeodomain protein in the ascidian Halocynthia roretzi functions in the differentiation and specification of neural cells in ascidian embryogenesis
    Y Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
    Mech Dev 104:37-48. 2001
    ..These results indicate that HrHNF-6 functions in the differentiation and regional specification of neural cells during ascidian embryogenesis...
  6. ncbi request reprint Large-scale cDNA analysis of the maternal genetic information in the egg of Halocynthia roretzi for a gene expression catalog of ascidian development
    K W Makabe
    Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Development 128:2555-67. 2001
    ..9% of the clones were exclusively maternal, while 40.6% of the maternal clones showed expression in the later stages. This study provides global insights about the genes expressed during early development...
  7. ncbi request reprint Gene expression profiles in Ciona intestinalis tailbud embryos
    Y Satou
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
    Development 128:2893-904. 2001
    ..The sequence information will also be used for further genome scientific approach to explore molecular mechanisms involved in the formation of one of the most primitive chordate body plans...
  8. ncbi request reprint Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis
    T Kawashima
    Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Dev Genes Evol 210:162-5. 2000
    ..Ascidian musashi had three domains of zygotic expression: the brain, nerve cord, and mesenchyma. The temporal order of the onset in these domains was highly divergent between the two species of ascidian examined...
  9. ncbi request reprint Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos
    Yasunori Sasakura
    Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan
    Dev Dyn 237:39-50. 2008
    ..This technique allowed us to isolate transgenic lines that express GFP in restricted tissues. This system provides an easy and efficient method for large-scale enhancer detection in Ciona intestinalis...
  10. pmc Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis
    Yasunori Sasakura
    Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415 0025, Japan
    Genome Biol 8:S3. 2007
    ..A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis...
  11. ncbi request reprint High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis
    Satoko Awazu
    Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan
    Genesis 45:307-17. 2007
    ..This high-throughput enhancer trap screen will be useful to create new marker transgenic lines showing reporter gene expression in specific tissues and to identify novel patterns of gene expression...
  12. pmc Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 102:15134-9. 2005
    ..It is likely that ascidian cellulose synthase is required for the coordination of the metamorphic events in the trunk and tail in addition to cellulose biosynthesis...
  13. ncbi request reprint Germline transgenesis of the ascidian Ciona intestinalis by electroporation
    Terumi Matsuoka
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto, Japan
    Genesis 41:67-72. 2005
    ....
  14. ncbi request reprint An enhancer trap in the ascidian Ciona intestinalis identifies enhancers of its Musashi orthologous gene
    Satoko Awazu
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Biol 275:459-72. 2004
    ..These results clearly demonstrate that an enhancer trap event that entrapped enhancers of Ci-Musashi occurred in C. intestinalis...
  15. ncbi request reprint Minos transposon causes germline transgenesis of the ascidian Ciona savignyi
    Terumi Matsuoka
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 46:249-55. 2004
    ..In addition, one insertion seemed to have caused an enhancer trapping. These results indicate that Minos provides a potential tool for transgenic techniques such as insertional mutagenesis in Ciona savignyi...
  16. pmc Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 100:7726-30. 2003
    ..In addition, an enhancer-trap line was obtained. This is a demonstration of efficient, Minos-mediated transgenesis in marine invertebrates...
  17. ncbi request reprint A genomewide survey of developmentally relevant genes in Ciona intestinalis. VII. Molecules involved in the regulation of cell polarity and actin dynamics
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Genes Evol 213:273-83. 2003
    ..Ciona intestinalis, therefore, may provide refined clues for the study of vertebrate development and evolution...
  18. ncbi request reprint A genomewide survey of developmentally relevant genes in Ciona intestinalis. X. Genes for cell junctions and extracellular matrix
    Yasunori Sasakura
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Genes Evol 213:303-13. 2003
    ..In vertebrates these ancestral cell junctions appear to have evolved into more diverse, and possibly more complex, forms, compared with those in their urochordate siblings...
  19. ncbi request reprint The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins
    Paramvir Dehal
    U S Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
    Science 298:2157-67. 2002
    ..The ascidian genome has also acquired a number of lineage-specific innovations, including a group of genes engaged in cellulose metabolism that are related to those in bacteria and fungi...
  20. ncbi request reprint A genomewide survey of developmentally relevant genes in Ciona intestinalis. V. Genes for receptor tyrosine kinase pathway and Notch signaling pathway
    Yutaka Satou
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Genes Evol 213:254-63. 2003
    ..The gene set identified in the present analysis provides an insight into genes for the RTK, MAPK and Notch signaling pathways in the ancient chordate genome and thereby how chordates evolved these signaling pathway...
  21. doi request reprint Euro chordates: Ascidian community swims ahead. The 4th International Tunicate meeting in Villefranche sur Mer
    Christian Sardet
    BioMarCell, UMR 7009, CNRS UPMC, Station Zoologique, Observatoire Oceanologique, Villefranche sur Mer, France
    Dev Dyn 237:1207-13. 2008
  22. ncbi request reprint A cDNA resource from the basal chordate Ciona intestinalis
    Yutaka Satou
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto, Japan
    Genesis 33:153-4. 2002
    ..This release is ready for use in cDNA cloning, micro/macroarray analysis, and other comprehensive genome-wide analyses for further molecular studies of basal chordates...
  23. ncbi request reprint Culture of Ciona intestinalis in closed systems
    Jean Stephane Joly
    INRA MSNC Group, DEPSN, UPR2197, Institut Fessard, CNRS, Gif sur Yvette, France
    Dev Dyn 236:1832-40. 2007
    ..Because these systems make it easier to rear Ciona intestinalis in laboratories, they increase the potentialities of this model organism for research...
  24. ncbi request reprint Postplasmic/PEM RNAs: a class of localized maternal mRNAs with multiple roles in cell polarity and development in ascidian embryos
    Francois Prodon
    Department of Biology, Graduate School of Science, Osaka University, Osaka, Japan
    Dev Dyn 236:1698-715. 2007
    ..Finally, we propose a speculative model integrating recent data, and we also discuss the relationship between postplasmic/PEM RNAs, posterior specification, and germ cell formation in ascidians...
  25. ncbi request reprint Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis
    Yasunori Sasakura
    Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan
    Dev Dyn 236:1758-67. 2007
    ..This review specifically addresses recent achievements of transformation techniques in Ciona, as exemplified using the Minos system...
  26. ncbi request reprint Tissue-specific profile of DNA replication in the swimming larvae of Ciona intestinalis
    Akie Nakayama
    Department of Zoology, Graduate School of Science, Kyoto University, Japan
    Zoolog Sci 22:301-9. 2005
    ..Our data showed that DNA synthesis in the larval trunk is not directly associated with the organization of adult organs, but it contributes to the speed of metamorphosis after settlement...
  27. pmc The amphioxus genome illuminates vertebrate origins and cephalochordate biology
    Linda Z Holland
    Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, California 92093 0202, USA
    Genome Res 18:1100-11. 2008
    ..Our results indicate that the amphioxus genome is elemental to an understanding of the biology and evolution of nonchordate deuterostomes, invertebrate chordates, and vertebrates...