Affiliation: Kyoto University
Takeya T, Yurimoto H, Sakai Y. A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanol. Appl Microbiol Biotechnol. 2018;102:7017-7027 pubmed publisher
..This biosensor can be used for high-throughput screening of single cells harboring high methanol-producing activity, and thereby, the development of a bioconversion process using methanol-producing enzymes. ..
Sakai Y, Murdanoto A, Konishi T, Iwamatsu A, Kato N. Regulation of the formate dehydrogenase gene, FDH1, in the methylotrophic yeast Candida boidinii and growth characteristics of an FDH1-disrupted strain on methanol, methylamine, and choline. J Bacteriol. 1997;179:4480-5 pubmed
Sakai Y, Yurimoto H, Matsuo H, Kato N. Regulation of peroxisomal proteins and organelle proliferation by multiple carbon sources in the methylotrophic yeast, Candida boidinii. Yeast. 1998;14:1175-87 pubmed
..These results indicate that peroxisomal proliferation in yeasts is not necessarily sensitive to glucose repression. Lastly, this regulation was shown to occur at the mRNA level. ..
Oda S, Yurimoto H, Nitta N, Sasano Y, Sakai Y. Molecular characterization of hap complex components responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii. Eukaryot Cell. 2015;14:278-85 pubmed publisher
..boidinii. Our results shed light on a novel function of the Hap complex in methanol-inducible gene expression in methylotrophic yeasts. ..
Shiraishi K, Oku M, Uchida D, Yurimoto H, Sakai Y. Regulation of nitrate and methylamine metabolism by multiple nitrogen sources in the methylotrophic yeast Candida boidinii. FEMS Yeast Res. 2015;15: pubmed publisher
..These regulatory profiles may reflect life style of nitrogen utilization in this yeast living in the phyllosphere. ..
Oku M, Sakai Y. Pexophagy in yeasts. Biochim Biophys Acta. 2016;1863:992-8 pubmed publisher
..We also discuss the physiological importance of pexophagy in these yeast systems. ..
Sakai Y, Nakagawa T, Shimase M, Kato N. Regulation and physiological role of the DAS1 gene, encoding dihydroxyacetone synthase, in the methylotrophic yeast Candida boidinii. J Bacteriol. 1998;180:5885-90 pubmed
Sakai Y, Oku M, van der Klei I, Kiel J. Pexophagy: autophagic degradation of peroxisomes. Biochim Biophys Acta. 2006;1763:1767-75 pubmed
..Subsequently, the engulfed peroxisome cluster is degraded. This review discusses the current state of knowledge of pexophagy with emphasis on studies on methylotrophic yeast species. ..
Iguchi H, Yurimoto H, Sakai Y. Interactions of Methylotrophs with Plants and Other Heterotrophic Bacteria. Microorganisms. 2015;3:137-51 pubmed publisher
..g., cobalamin). Information regarding the biological interaction of methylotrophs with other organisms will facilitate a better understanding of the carbon cycle that is driven by methylotrophs. ..