Research Topics
| Keisuke OkitaSummaryAffiliation: Kyoto University Country: Japan Publications
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Detail Information
Publications
A more efficient method to generate integration-free human iPS cellsKeisuke Okita
Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
Nat Methods 8:409-12. 2011..This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future...- iPS cells for transplantationKeisuke Okita
Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
Curr Opin Organ Transplant 16:96-100. 2011..However, there are still several problems to be solved in terms of safety concerns. We herein summarize the current knowledge about iPS cells and the obstacles that must be overcome before the cells can be used for medical applications... - Generation of mouse-induced pluripotent stem cells with plasmid vectorsKeisuke Okita
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto, Japan
Nat Protoc 5:418-28. 2010..This virus-free technique reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency... - Induction of pluripotency by defined factorsKeisuke Okita
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Exp Cell Res 316:2565-70. 2010..On the other hand, patient-specific iPS cells have already been established and used for recapitulating disease pathology... - Generation of pluripotent stem cells from adult mouse liver and stomach cellsTakashi Aoi
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Science 321:699-702. 2008..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required... 
Suppression of induced pluripotent stem cell generation by the p53-p21 pathwayHyenjong Hong
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Nature 460:1132-5. 2009..Functional analyses of these genes demonstrate that the p53-p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation...- Generation of mouse induced pluripotent stem cells without viral vectorsKeisuke Okita
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Science 322:949-53. 2008..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine... - Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblastsNoriko Tsubooka
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Genes Cells 14:683-94. 2009..These results demonstrated that Sall4 plays positive roles in the generation of pluripotent stem cells from blastocysts and fibroblasts... 
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblastsMasato Nakagawa
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Nat Biotechnol 26:101-6. 2008..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...- Variation in the safety of induced pluripotent stem cell linesKyoko Miura
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
Nat Biotechnol 27:743-5. 2009..In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells... - Orderly hematopoietic development of induced pluripotent stem cells via Flk-1(+) hemoangiogenic progenitorsAkira Niwa
Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
J Cell Physiol 221:367-77. 2009..This finding highlights the potential use of iPS cells in clinical areas such as regenerative medicine, disease investigation, and drug screening... - Induction of pluripotent stem cells from fibroblast culturesKazutoshi Takahashi
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
Nat Protoc 2:3081-9. 2007..The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells... - ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotencyKumiko A Iwabuchi
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
PLoS ONE 6:e20461. 2011..However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression... - Methods for iPS cell generation for basic research and clinical applicationsYuji Mochiduki
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
Biotechnol J 7:789-97. 2012..Here we summarize the current status of iPS cell studies, with a focus on the improved methods that can be used to generate iPS cells, and also refer to the future challenges... - Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitorsKunio Hirano
Laboratory of Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Stem Cells Dev 21:1287-98. 2012..Thus, 2i-hiPSCs, which are nonteratoma forming PNSCs, may represent a safe source of cells for neural research and regenerative medicine... - Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-AMasataka Fujiwara
Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
PLoS ONE 6:e16734. 2011..These results provide a technological basis to obtain functional cardiomyocytes from iPSCs... - Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cellsGenta Narazaki
Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Circulation 118:498-506. 2008..However, detailed differentiation properties and the directional differentiation system of iPS cells have not been demonstrated... - An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cellsKeisuke Okita
Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
Stem Cells 31:458-66. 2013..This induction method will be useful for the derivation of patient-specific integration-free iPSCs and would also be applicable to the generation of clinical-grade iPSCs in the future... - Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cellsMasatoshi Kajiwara
Center for Induced Pluripotent Stem Cell Research and Application, Department of Surgery, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Proc Natl Acad Sci U S A 109:12538-43. 2012..These data underscore the importance of donor differences when comparing the differentiation propensities of hiPSC clones... - Transplantation of mouse induced pluripotent stem cells into the cochleaKoji Nishimura
Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
Neuroreport 20:1250-4. 2009..Some transplants expressed vesicular glutamate transporter 1, which is a marker for glutamatergic neurons. These findings indicate that iPS cells can be used as a source of transplants for the regeneration of spiral ganglion neurons... - Modeling Alzheimer's Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug ResponsivenessTakayuki Kondo
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan Core Research for Evolutional Science and Technology CREST, Japan Science and Technology Agency JST, Saitama 332 0012, Japan
Cell Stem Cell 12:487-96. 2013..It also illustrates how patient-specific iPSCs can be useful for analyzing AD pathogenesis and evaluating drugs... - Generation of germline-competent induced pluripotent stem cellsKeisuke Okita
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Nature 448:313-7. 2007..Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application... - Drug screening for ALS using patient-specific induced pluripotent stem cellsNaohiro Egawa
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
Sci Transl Med 4:145ra104. 2012..These findings suggest that motor neurons generated from ALS patient-derived iPSCs may provide a useful tool for elucidating ALS disease pathogenesis and for screening drug candidates... - Induced pluripotent stem cells: opportunities and challengesKeisuke Okita
Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
Philos Trans R Soc Lond B Biol Sci 366:2198-207. 2011..This report will summarize the current knowledge of iPS generation and discuss future reprogramming methods for medical application... - Intracellular signaling pathways regulating pluripotency of embryonic stem cellsKeisuke Okita
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
Curr Stem Cell Res Ther 1:103-11. 2006..We will review the rapid progress that has been made in identifying and dissecting the intracellular signaling pathways that contribute to self-renewal of pluripotent mouse and human ES cells...