Keisuke Okita

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint A more efficient method to generate integration-free human iPS cells
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Nat Methods 8:409-12. 2011
  2. doi request reprint iPS cells for transplantation
    Keisuke Okita
    Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Curr Opin Organ Transplant 16:96-100. 2011
  3. doi request reprint Generation of mouse-induced pluripotent stem cells with plasmid vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto, Japan
    Nat Protoc 5:418-28. 2010
  4. doi request reprint Induction of pluripotency by defined factors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Exp Cell Res 316:2565-70. 2010
  5. pmc Suppression of induced pluripotent stem cell generation by the p53-p21 pathway
    Hyenjong Hong
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:1132-5. 2009
  6. doi request reprint Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
  7. doi request reprint Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
  8. doi request reprint Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts
    Noriko Tsubooka
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Genes Cells 14:683-94. 2009
  9. doi request reprint Response to Comment on "Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells"
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 5:188lr2. 2013
  10. ncbi request reprint Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008

Collaborators

Detail Information

Publications30

  1. doi request reprint A more efficient method to generate integration-free human iPS cells
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Nat Methods 8:409-12. 2011
    ..This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future...
  2. doi request reprint iPS cells for transplantation
    Keisuke Okita
    Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Curr Opin Organ Transplant 16:96-100. 2011
    ..However, there are still several problems to be solved in terms of safety concerns. We herein summarize the current knowledge about iPS cells and the obstacles that must be overcome before the cells can be used for medical applications...
  3. doi request reprint Generation of mouse-induced pluripotent stem cells with plasmid vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto, Japan
    Nat Protoc 5:418-28. 2010
    ..This virus-free technique reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency...
  4. doi request reprint Induction of pluripotency by defined factors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Exp Cell Res 316:2565-70. 2010
    ..On the other hand, patient-specific iPS cells have already been established and used for recapitulating disease pathology...
  5. pmc Suppression of induced pluripotent stem cell generation by the p53-p21 pathway
    Hyenjong Hong
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:1132-5. 2009
    ..Functional analyses of these genes demonstrate that the p53-p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation...
  6. doi request reprint Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  7. doi request reprint Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
    ..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine...
  8. doi request reprint Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts
    Noriko Tsubooka
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Genes Cells 14:683-94. 2009
    ..These results demonstrated that Sall4 plays positive roles in the generation of pluripotent stem cells from blastocysts and fibroblasts...
  9. doi request reprint Response to Comment on "Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells"
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 5:188lr2. 2013
    ..Our work and the study of Bilican et al. highlight the need for complementary assays to detect subtle phenotypic differences between control and mutant induced pluripotent stem cell lines...
  10. ncbi request reprint Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    ..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...
  11. pmc Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells
    Michiyo Koyanagi-Aoi
    Center for iPS Cell Research and Application, Department of Biological Repair, Institute for Frontier Medical Sciences, and Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 110:20569-74. 2013
    ..These data demonstrated a subset of hiPSC lines that have aberrant gene expression and defective potential in neural differentiation, which need to be identified and eliminated before applications in regenerative medicine. ..
  12. doi request reprint Variation in the safety of induced pluripotent stem cell lines
    Kyoko Miura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Nat Biotechnol 27:743-5. 2009
    ..In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells...
  13. doi request reprint Orderly hematopoietic development of induced pluripotent stem cells via Flk-1(+) hemoangiogenic progenitors
    Akira Niwa
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Cell Physiol 221:367-77. 2009
    ..This finding highlights the potential use of iPS cells in clinical areas such as regenerative medicine, disease investigation, and drug screening...
  14. ncbi request reprint Induction of pluripotent stem cells from fibroblast cultures
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Protoc 2:3081-9. 2007
    ..The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells...
  15. doi request reprint An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells
    Keisuke Okita
    Department of Reprogramming Science, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Stem Cells 31:458-66. 2013
    ..This induction method will be useful for the derivation of patient-specific integration-free iPSCs and would also be applicable to the generation of clinical-grade iPSCs in the future...
  16. pmc ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency
    Kumiko A Iwabuchi
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e20461. 2011
    ..However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression...
  17. doi request reprint Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Aβ and differential drug responsiveness
    Takayuki Kondo
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 12:487-96. 2013
    ..It also illustrates how patient-specific iPSCs can be useful for analyzing AD pathogenesis and evaluating drugs...
  18. doi request reprint Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitors
    Kunio Hirano
    Laboratory of Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Stem Cells Dev 21:1287-98. 2012
    ..Thus, 2i-hiPSCs, which are nonteratoma forming PNSCs, may represent a safe source of cells for neural research and regenerative medicine...
  19. doi request reprint Methods for iPS cell generation for basic research and clinical applications
    Yuji Mochiduki
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Biotechnol J 7:789-97. 2012
    ..Here we summarize the current status of iPS cell studies, with a focus on the improved methods that can be used to generate iPS cells, and also refer to the future challenges...
  20. doi request reprint Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells
    Genta Narazaki
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Circulation 118:498-506. 2008
    ..However, detailed differentiation properties and the directional differentiation system of iPS cells have not been demonstrated...
  21. pmc Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A
    Masataka Fujiwara
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e16734. 2011
    ..These results provide a technological basis to obtain functional cardiomyocytes from iPSCs...
  22. doi request reprint Generation and characterization of induced pluripotent stem cells from aid-deficient mice
    Ren Shimamoto
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    PLoS ONE 9:e94735. 2014
    ..A comprehensive DNA methylation analysis showed only a few differences between Aid+/+ and Aid-/- iPS cells. These data suggest that Aid does not have crucial functions in DNA demethylation during iPS cell generation. ..
  23. ncbi request reprint Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    ..Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application...
  24. pmc Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells
    Masatoshi Kajiwara
    Center for Induced Pluripotent Stem Cell Research and Application, Department of Surgery, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 109:12538-43. 2012
    ..These data underscore the importance of donor differences when comparing the differentiation propensities of hiPSC clones...
  25. doi request reprint Transplantation of mouse induced pluripotent stem cells into the cochlea
    Koji Nishimura
    Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Neuroreport 20:1250-4. 2009
    ..Some transplants expressed vesicular glutamate transporter 1, which is a marker for glutamatergic neurons. These findings indicate that iPS cells can be used as a source of transplants for the regeneration of spiral ganglion neurons...
  26. doi request reprint Drug screening for ALS using patient-specific induced pluripotent stem cells
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 4:145ra104. 2012
    ..These findings suggest that motor neurons generated from ALS patient-derived iPSCs may provide a useful tool for elucidating ALS disease pathogenesis and for screening drug candidates...
  27. pmc Induced pluripotent stem cells: opportunities and challenges
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Philos Trans R Soc Lond B Biol Sci 366:2198-207. 2011
    ..This report will summarize the current knowledge of iPS generation and discuss future reprogramming methods for medical application...
  28. ncbi request reprint Intracellular signaling pathways regulating pluripotency of embryonic stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Curr Stem Cell Res Ther 1:103-11. 2006
    ..We will review the rapid progress that has been made in identifying and dissecting the intracellular signaling pathways that contribute to self-renewal of pluripotent mouse and human ES cells...
  29. doi request reprint Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells
    Sou Nakamura
    Department of Clinical Application, Center for iPS Cell Research and Application CiRA, Kyoto University, 606 8507, Japan
    Cell Stem Cell 14:535-48. 2014
    ..The combination of robust expansion capacity and efficient platelet production means that appropriately selected imMKCL clones represent a potentially inexhaustible source of hPSC-derived platelets for clinical application. ..
  30. doi request reprint Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation
    Kotaro Ohnishi
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan Department of Medicine, Gifu University Graduate School of Medicine, Gifu 501 1194, Japan
    Cell 156:663-77. 2014
    ..These findings suggest that epigenetic regulation associated with iPSC derivation may drive development of particular types of cancer...