Naoki Okada

Summary

Affiliation: Kyoto Pharmaceutical University
Country: Japan

Publications

  1. ncbi Cell delivery system: a novel strategy to improve the efficacy of cancer immunotherapy by manipulation of immune cell trafficking and biodistribution
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Kyoto, Japan
    Biol Pharm Bull 28:1543-50. 2005
  2. ncbi [Design and creation of cytomedicine for application to cell therapy]
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto, Japan
    Yakugaku Zasshi 125:601-15. 2005
  3. ncbi Tumor suppressive efficacy through augmentation of tumor-infiltrating immune cells by intratumoral injection of chemokine-expressing adenoviral vector
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto, Japan
    Cancer Gene Ther 13:393-405. 2006
  4. ncbi Transcriptional targeting of RGD fiber-mutant adenovirus vectors can improve the safety of suicide gene therapy for murine melanoma
    Yuka Okada
    Research Institute for Microbial Diseases, Osaka University, Japan
    Cancer Gene Ther 12:608-16. 2005
  5. ncbi Anti-tumor activity of chemokine is affected by both kinds of tumors and the activation state of the host's immune system: implications for chemokine-based cancer immunotherapy
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Biochem Biophys Res Commun 317:68-76. 2004
  6. ncbi Gene transduction efficiency and maturation status in mouse bone marrow-derived dendritic cells infected with conventional or RGD fiber-mutant adenovirus vectors
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Cancer Gene Ther 10:421-31. 2003
  7. ncbi Efficient antigen gene transduction using Arg-Gly-Asp fiber-mutant adenovirus vectors can potentiate antitumor vaccine efficacy and maturation of murine dendritic cells
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto 607 8414, Japan
    Cancer Res 61:7913-9. 2001
  8. ncbi Effects of lipofectin-antigen complexes on major histocompatibility complex class I-restricted antigen presentation pathway in murine dendritic cells and on dendritic cell maturation
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Japan
    Biochim Biophys Acta 1527:97-101. 2001
  9. ncbi Immunological properties and vaccine efficacy of murine dendritic cells simultaneously expressing melanoma-associated antigen and interleukin-12
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Cancer Gene Ther 12:72-83. 2005
  10. ncbi Adenovirus vector covalently conjugated to polyethylene glycol with a cancer-specific promoter suppresses the tumor growth through systemic administration
    Xinglei Yao
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Biol Pharm Bull 33:1073-6. 2010

Collaborators

Detail Information

Publications108 found, 100 shown here

  1. ncbi Cell delivery system: a novel strategy to improve the efficacy of cancer immunotherapy by manipulation of immune cell trafficking and biodistribution
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Kyoto, Japan
    Biol Pharm Bull 28:1543-50. 2005
    ..This review introduces our novel "cell delivery system," which employs an Arg-Gly-Asp (RGD) fiber-mutant adenovirus vector encoding the chemokine or chemokine receptor gene in cancer immunotherapy...
  2. ncbi [Design and creation of cytomedicine for application to cell therapy]
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto, Japan
    Yakugaku Zasshi 125:601-15. 2005
    ..This review introduces our approach to the design and creation of cytomedicine for application to cell therapy against diabetes mellitus and cancer...
  3. ncbi Tumor suppressive efficacy through augmentation of tumor-infiltrating immune cells by intratumoral injection of chemokine-expressing adenoviral vector
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto, Japan
    Cancer Gene Ther 13:393-405. 2006
    ....
  4. ncbi Transcriptional targeting of RGD fiber-mutant adenovirus vectors can improve the safety of suicide gene therapy for murine melanoma
    Yuka Okada
    Research Institute for Microbial Diseases, Osaka University, Japan
    Cancer Gene Ther 12:608-16. 2005
    ..These results indicate that AdRGD combined with transcriptional regulation using Tyr or TERT promoter is a potentially useful and safe vector system for suicide gene therapy for melanoma...
  5. ncbi Anti-tumor activity of chemokine is affected by both kinds of tumors and the activation state of the host's immune system: implications for chemokine-based cancer immunotherapy
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Biochem Biophys Res Commun 317:68-76. 2004
    ..Altogether, our results suggest that the tumor-suppressive activity of chemokine-gene immunotherapy is greatly influenced by the kind of tumor and the activation state of the host's immune system...
  6. ncbi Gene transduction efficiency and maturation status in mouse bone marrow-derived dendritic cells infected with conventional or RGD fiber-mutant adenovirus vectors
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Cancer Gene Ther 10:421-31. 2003
    ..AdRGD-infection caused greater induction of maturation than conventional Ad-infection, irrespective of the type of transgene inserted...
  7. ncbi Efficient antigen gene transduction using Arg-Gly-Asp fiber-mutant adenovirus vectors can potentiate antitumor vaccine efficacy and maturation of murine dendritic cells
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto 607 8414, Japan
    Cancer Res 61:7913-9. 2001
    ....
  8. ncbi Effects of lipofectin-antigen complexes on major histocompatibility complex class I-restricted antigen presentation pathway in murine dendritic cells and on dendritic cell maturation
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Japan
    Biochim Biophys Acta 1527:97-101. 2001
    ..4 cells. These findings suggest that an antigen-loading procedure using LF could enhance delivery of exogenous antigens to the classical MHC class I pathway in DCs, but it does not initiate DC maturation...
  9. ncbi Immunological properties and vaccine efficacy of murine dendritic cells simultaneously expressing melanoma-associated antigen and interleukin-12
    Naoki Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Cancer Gene Ther 12:72-83. 2005
    ....
  10. ncbi Adenovirus vector covalently conjugated to polyethylene glycol with a cancer-specific promoter suppresses the tumor growth through systemic administration
    Xinglei Yao
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Biol Pharm Bull 33:1073-6. 2010
    ..These results demonstrated that PEG-Ad-TERT can be regarded as a prototype Adv with suitable efficacy and safety for systemic cancer gene therapy against both metastatic and primary tumors...
  11. doi A novel strategy utilizing ultrasound for antigen delivery in dendritic cell-based cancer immunotherapy
    Ryo Suzuki
    Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Sagamiko cho, Sagamihara, Kanagawa, Japan
    J Control Release 133:198-205. 2009
    ..G7-OVA tumors. These data indicate that the combination of BLs and US exposure is a promising antigen delivery system in DC-based cancer immunotherapy...
  12. doi Comparison of the anti-tumor activity of native, secreted, and membrane-bound LIGHT in mouse tumor models
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Int Immunopharmacol 10:26-33. 2010
    ..These results suggest that the secreted form of LIGHT might be the optimal form for cancer gene therapy...
  13. doi Suppression of nanosilica particle-induced inflammation by surface modification of the particles
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan
    Arch Toxicol 86:1297-307. 2012
    ..These results provide basic information that will be useful for the development of safe nanomaterials...
  14. ncbi TERT promoter-driven adenovirus vector for cancer gene therapy via systemic injection
    Xinglei Yao
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6, Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 362:419-24. 2007
    ..Our results suggest that Adv in which transgene expression is driven by the TERT promoter are a promising prototype of tumor-targeting vectors for effective and safe cancer gene therapy...
  15. ncbi Functional and molecular identification of sodium-coupled dicarboxylate transporters in rat primary cultured cerebrocortical astrocytes and neurons
    Etsuo Yodoya
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Yamashina ku, Kyoto, Japan
    J Neurochem 97:162-73. 2006
    ..These transporters might play important roles in the trafficking of tricarboxylic acid cycle intermediates and related metabolites between glia and neurons...
  16. doi LIGHT protein suppresses tumor growth by augmentation of immune response
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6, Yamadaoka, Suita, Osaka, 565 0871, Japan
    Immunol Lett 127:33-8. 2009
    ..These results indicate that LIGHT protein efficiently evokes the systemic tumor-specific immune response, and thus induces tumor suppression...
  17. doi Nanoparticles built by self-assembly of amphiphilic gamma-PGA can deliver antigens to antigen-presenting cells with high efficiency: a new tumor-vaccine carrier for eliciting effector T cells
    Tomoaki Yoshikawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Vaccine 26:1303-13. 2008
    ..Our results demonstrate that a gamma-PGA NP system for antigen delivery will advance the clinical utility of vaccines against cancer...
  18. pmc Tumor vascular targeted delivery of polymer-conjugated adenovirus vector for cancer gene therapy
    Xinglei Yao
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    Mol Ther 19:1619-25. 2011
    ..These results indicate that Adv-PEG(CGKRK) has potential as a prototype Adv with suitable efficacy and safety for systemic cancer gene therapy against both primary tumors and metastases...
  19. doi The effect of surface modification of amorphous silica particles on NLRP3 inflammasome mediated IL-1beta production, ROS production and endosomal rupture
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biomaterials 31:6833-42. 2010
    ..Our results reveal a part of NLRP3 activation pathway and provide basic information that should help to create safe and effective forms of SPs...
  20. ncbi The utility of poly(γ-glutamic acid) nanoparticles as antigen delivery carriers in dendritic cell-based cancer immunotherapy
    Keisuke Matsuo
    Department of Biotechnology and Therapeutics, Osaka University, Japan
    Biol Pharm Bull 33:2003-7. 2010
    ..Immunization with TAA-loaded DCs using γ-PGA NPs inhibited tumor growth by inducing TAA-specific CTLs. These findings indicate that γ-PGA NPs can function as useful antigen delivery carriers in DC-based cancer immunotherapy...
  21. doi Antitumor mechanism of intratumoral injection with IL-12-expressing adenoviral vector against IL-12-unresponsive tumor
    Naoko Kanagawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 372:821-5. 2008
    ..The present data provide evidence that the antitumor effect of IL-12 gene transduction in a local tumor site is dependent on induced alterations of the tumor microenvironment and systemic immunity...
  22. doi Robo4 is an effective tumor endothelial marker for antibody-drug conjugates based on the rapid isolation of the anti-Robo4 cell-internalizing antibody
    Mai Yoshikawa
    Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    Blood 121:2804-13. 2013
    ..These findings indicate that cell-internalizing activity plays an important role in the biodistribution and therapeutic effects of ADCs. Further, Robo4 can be an effective marker for tumor vascular targeting...
  23. ncbi Excellent absorption enhancing characteristics of NO donors for improving the intestinal absorption of poorly absorbable compound compared with conventional absorption enhancers
    Gihan Fetih
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Japan
    Drug Metab Pharmacokinet 21:222-9. 2006
    ..They can enhance intestinal absorption of CF from all intestinal regions and they are effective at very low concentrations...
  24. doi Development and clinical study of a self-dissolving microneedle patch for transcutaneous immunization device
    Sachiko Hirobe
    Laboratory of Biotechnology and Therapeutics Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Pharm Res 30:2664-74. 2013
    ..In this study, we developed an improved collagen-free MH (new-MH) and conducted clinical study to evaluate the fundamental properties and safety in human...
  25. ncbi [Development of effective antigen delivery carrier to dendritic cells via Fc receptor in cancer immunotherapy]
    Ryo Suzuki
    Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Sagamiko cho, Sagamihara City, Japan
    Yakugaku Zasshi 127:301-6. 2007
    ..These results suggest that IgG-liposomes represent an efficient antigen delivery carrier for DCs in cancer immunotherapy...
  26. doi Mutants of lymphotoxin-α with augmented cytotoxic activity via TNFR1 for use in cancer therapy
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Cytokine 61:578-84. 2013
    ..Our data suggest that the kinetics of LTα binding to TNFR1 play an important role in signal transduction patterns, and a TNFR1-selective LTα mutant with augmented bioactivity would be a superior candidate for cancer therapy...
  27. doi Induction of endoplasmic reticulum-endosome fusion for antigen cross-presentation induced by poly (γ-glutamic acid) nanoparticles
    Yohei Mukai
    Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565 0871, Japan
    J Immunol 187:6249-55. 2011
    ..These findings imply that the ER-endosome complex is key for the efficient cross-presentation of Ags encapsulated in γ-PGA NPs...
  28. doi Tat conjugation of adenovirus vector broadens tropism and enhances transduction efficiency
    Yasuo Yoshioka
    The Center for Advanced Medical Engineering and Informatics, Osaka University, 1 6, Yamadaoka, Suita, Osaka, 565 0871, Japan
    Life Sci 83:747-55. 2008
    ..In this study, we attempted to generate novel Tat-Advs (Advs conjugated with the HIV Tat-derived peptide, a protein-transduction domain (PTD)) to broaden Adv tropism and enhance transduction efficiency...
  29. ncbi A strategy for efficient cross-presentation of CTL-epitope peptides leading to enhanced induction of in vivo tumor immunity
    Akira Hayashi
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Control Release 117:11-9. 2007
    ..Thus, Eriss-conjugated CTL-epitope peptides encapsulated in FLs provide a highly efficient tumor-vaccine to enhance the induction of in vivo tumor immunity...
  30. ncbi Simple PEG conjugation of SPIO via an Au-S bond improves its tumor targeting potency as a novel MR tumor imaging agent
    Hiroki Kojima
    Center for Advanced Medical Engineering and Informatics, Osaka University, Suita, Japan
    Bioconjug Chem 21:1026-31. 2010
    ..In addition, because the synthesis of PEG-Au/Feridex is simple and high yields are easily produced, PEG-modified SPIO for tumor diagnosis can be prepared on an industrial scale with low cost...
  31. ncbi Functional linkage of H+/peptide transporter PEPT2 and Na+/H+ exchanger in primary cultures of astrocytes from mouse cerebral cortex
    Miyuki Wada
    Department of Biochemical Pharmacology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607 8414, Japan
    Brain Res 1044:33-41. 2005
    ..This study represents the first description of the functional co-operation of PEPT2 and NHE1 and/or NHE2 in cerebrocortical astrocytes...
  32. ncbi A novel cytomedical vehicle capable of protecting cells against complement
    Yasuo Yoshioka
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 305:353-8. 2003
    ..Therefore, APA(Ba) microbeads may be a useful carrier for developing anti-complement device for cytomedical therapy...
  33. doi Development of a novel therapeutic approach using a retinoic acid-loaded microneedle patch for seborrheic keratosis treatment and safety study in humans
    Yasuhiro Hiraishi
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Control Release 171:93-103. 2013
    ..Overall, MN patches can offer an effective and safe means of ATRA delivery into the skin, and the ATRA-loaded MN patch appears to be an effective pharmaceutical product providing a novel therapeutic option for seborrheic keratosis...
  34. doi Factors involved in the maturation of murine dendritic cells transduced with adenoviral vector variants
    Naoko Kanagawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Virology 374:411-20. 2008
    ..Thus, our data provide evidence that Ad-induced DC maturation is due to Ad invasion of the DCs, followed by nuclear transport of the Ad genome, and not to the expression of foreign genes...
  35. ncbi Compositional optimization and safety assessment of a hydrogel patch as a transcutaneous immunization device
    Kazuhiko Matsuo
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565 0871, Japan
    Biol Pharm Bull 34:1835-40. 2011
    ..TCI using a hydrogel patch induced few local and systemic adverse reactions. Based on these results, we are now advancing translational research to evaluate the safety and efficacy of our novel TCI system in humans...
  36. ncbi [Development of pegylated adenovirus vector for cancer gene therapy]
    Yusuke Eto
    Guraduate School of Pharmaceutical Siences, Osaka University, Suita City, Japan
    Yakugaku Zasshi 128:1733-42. 2008
    ..In this symposium, I will present our approach for development of cancer targeted Ad by PEGylation...
  37. doi Intranasal immunization with poly(γ-glutamic acid) nanoparticles entrapping antigenic proteins can induce potent tumor immunity
    Keisuke Matsuo
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Control Release 152:310-6. 2011
    ..Thus, nasal vaccination with antigen-entrapping γ-PGA NPs evokes tumor immunity by eliciting antigen-specific CTLs. γ-PGA NPs are a promising antigen delivery carrier for the development of non-invasive cancer vaccines...
  38. doi Creation of a lysine-deficient LIGHT mutant with the capacity for site-specific PEGylation and low affinity for a decoy receptor
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 393:888-93. 2010
    ..Furthermore, mLIGHT-Lys(-) was not trapped by the nonfunctional DcR3, despite binding to its functional receptors. These results suggest that mLIGHT-Lys(-) might be a useful candidate for cancer therapy...
  39. doi A transcutaneous vaccination system using a hydrogel patch for viral and bacterial infection
    Yumiko Ishii
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Control Release 131:113-20. 2008
    ..The use of this hydrogel patch transcutaneous vaccination system will facilitate the global distribution of effective and convenient vaccines...
  40. doi Analysis of transcutaneous antigenic protein delivery by a hydrogel patch formulation
    Kazuhiko Matsuo
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565 0871, Japan
    J Pharm Sci 102:1936-47. 2013
    ..On the basis of the present information, we are planning to modify the patch composition and establish the antigen modification technology for improvement in the efficacy of transcutaneous immunization...
  41. ncbi CC-chemokine ligand 17 gene therapy induces tumor regression through augmentation of tumor-infiltrating immune cells in a murine model of preexisting CT26 colon carcinoma
    Naoko Kanagawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka, Japan
    Int J Cancer 121:2013-22. 2007
    ..Thus, our data provide evidence that CCL17 gene transduction of local tumor sites is a promising approach for the development of a cancer immunogene therapy that can recruit activated tumor-infiltrating immune effector cells...
  42. doi Performance and characteristics evaluation of a sodium hyaluronate-based microneedle patch for a transcutaneous drug delivery system
    Yasuhiro Hiraishi
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Int J Pharm 441:570-9. 2013
    ..Overall, although the strength of MN requires improvement, our developed MN patch appears to be an effective pharmaceutical product providing a simple, safe, and relatively painless approach...
  43. doi A low-invasive and effective transcutaneous immunization system using a novel dissolving microneedle array for soluble and particulate antigens
    Kazuhiko Matsuo
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Control Release 161:10-7. 2012
    ..In addition, application of these dissolving microneedle arrays caused only slight skin irritation. These findings suggest that our TCI system can simply, safely, and effectively improve protective immune responses for every vaccine Ag...
  44. doi Clinical study of transcutaneous vaccination using a hydrogel patch for tetanus and diphtheria
    Sachiko Hirobe
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Vaccine 30:1847-54. 2012
    ..This easy-to-use and safe TCI formulation enables mass treatment in an outbreak setting and increased vaccination rates in developing countries, and will greatly contribute to worldwide countermeasures against infectious diseases...
  45. doi Structure-activity relationship of T-cell receptors based on alanine scanning
    Shogo Narimatsu
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6, Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 415:558-62. 2011
    ..Our results provide fundamental information for constructing mutant TCRs with high binding affinity...
  46. doi Modulating effect of polyethylene glycol on the intestinal transport and absorption of prednisolone, methylprednisolone and quinidine in rats by in-vitro and in-situ absorption studies
    Qi Shen
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi Yamashina ku, Kyoto 607 8414 Japan
    J Pharm Pharmacol 60:1633-41. 2008
    ..1-1.0% (w/v) PEG 20000. These findings suggest that PEG 20000 might be a useful excipient to improve the intestinal absorption of quinidine, which is mainly secreted by a P-gp-mediated efflux system in the intestine...
  47. ncbi Development of amphiphilic gamma-PGA-nanoparticle based tumor vaccine: potential of the nanoparticulate cytosolic protein delivery carrier
    Tomoaki Yoshikawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 366:408-13. 2008
    ..Our findings suggest that the gamma-PGA NP system is suitable for the intracellular delivery of protein-based drugs as well as tumor vaccines...
  48. ncbi Chitosan oligomers as potential and safe absorption enhancers for improving the pulmonary absorption of interferon-alpha in rats
    Keigo Yamada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    J Pharm Sci 94:2432-40. 2005
    ..Therefore, these findings indicated that the use of chitosan oligomers would be a promising approach for improving of the pulmonary absorption of biologically active peptides including IFN...
  49. ncbi Vaccine efficacy of fusogenic liposomes containing tumor cell-lysate against murine B16BL6 melanoma
    Tomoaki Yoshikawa
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University
    Biol Pharm Bull 29:100-4. 2006
    ..Our results suggest that this flexible system is a promising approach for the development of versatile cancer immunotherapy regimes...
  50. doi Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Biomaterials 31:3357-63. 2010
    ..Our data provide valuable information for construction of more functional LIGHT mutants that might be powerful tools for cancer therapy...
  51. doi Transcutaneous vaccination using a hydrogel patch induces effective immune responses to tetanus and diphtheria toxoid in hairless rat
    Kazuhiko Matsuo
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Control Release 149:15-20. 2011
    ..On the basis of these results, we are now performing translational research to apply TCI for tetanus and diphtheria...
  52. ncbi Development of a novel cytomedical treatment that can protect entrapped cells from host humoral immunity
    Ryo Suzuki
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565 0871, Japan
    Cell Transplant 11:787-97. 2002
    ..These results suggest that PVS inhibits C1 in the classical pathway and C3 convertase formation in the alternative pathway. Therefore, PVS may be a useful polymer for developing an anticomplement device for cytomedical therapy...
  53. doi Tumor-targeting CTL expressing a single-chain Fv specific for VEGFR2
    Naoko Kanagawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 394:54-8. 2010
    ..These findings indicate that the creation of CTL capable of targeting tumor vascular endothelial cells by scFv-expression technique is considerably promising for improvement of efficacy in adoptive immunotherapy...
  54. ncbi Functional characterization of Na+-independent choline transport in primary cultures of neurons from mouse cerebral cortex
    Takuya Fujita
    Department of Biochemical Pharmacology, Kyoto Pharmaceutical University, Misasagi, Kyoto 607 8414, Japan
    Neurosci Lett 393:216-21. 2006
    ..This choline transport system seems to have relevance not only for neuronal physiology but also for the uptake of pharmacologically important organic cation drugs...
  55. ncbi Characterization of transcutaneous protein delivery by a hydrogel patch in animal, human, and tissue-engineered skin models
    Kazuhiko Matsuo
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka, Suita, Japan
    Biol Pharm Bull 34:586-9. 2011
    ..On the basis of these results, translational research in human is now in progress...
  56. ncbi Effective tumor targeted gene transfer using PEGylated adenovirus vector via systemic administration
    Jian Qing Gao
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    J Control Release 122:102-10. 2007
    ..PEGylation of Ad achieved tumor targeting through the EPR effect, and these attributes suggest that systemic injection of PEG-Ad has great potential as an anti-tumor treatment...
  57. ncbi Anti-tumor responses induced by chemokine CCL19 transfected into an ovarian carcinoma model via fiber-mutant adenovirus vector
    Jian Qing Gao
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
    Biol Pharm Bull 28:1066-70. 2005
    ....
  58. doi Lysine-deficient lymphotoxin-α mutant for site-specific PEGylation
    Shogo Narimatsu
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Cytokine 56:489-93. 2011
    ..These results suggest that site-specific PEGylated LT-K0 may be useful in cancer therapy...
  59. doi Titanium dioxide induces different levels of IL-1beta production dependent on its particle characteristics through caspase-1 activation mediated by reactive oxygen species and cathepsin B
    Tomohiro Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 392:160-5. 2010
    ..Our results provide basic information for the creation of safe and effective novel forms of TiO2...
  60. ncbi Optimized PEGylated adenovirus vector reduces the anti-vector humoral immune response against adenovirus and induces a therapeutic effect against metastatic lung cancer
    Yusuke Eto
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    Biol Pharm Bull 33:1540-4. 2010
    ..These results suggest that systemic administration of PEG-Ad with an appropriate PEG modification rate has the potential to reduce the production of antibodies against Adv and increase the therapeutic response against metastatic cancer...
  61. ncbi Non-methylated CpG motif packaged into fusogenic liposomes enhance antigen-specific immunity in mice
    Tomoaki Yoshikawa
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
    Biol Pharm Bull 29:105-9. 2006
    ..Thus, the development of a CpG-enhanced DNA-FL genetic immunization system represents a promising tool for developing candidate vaccines against some of the more difficult infectious, parasitic, and oncologic disease targets...
  62. doi Transduction of adenovirus vectors modified with cell-penetrating peptides
    Yusuke Eto
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6, Yamadaoka, Suita, Osaka 565 0871, Japan
    Peptides 30:1548-52. 2009
    ..The results obtained in this research encourage the use of CPP-peptide-modified Advs as an attractive tool for transducing cells and as useful platform vectors for gene therapy and basic research...
  63. ncbi Dendritic cells that endocytosed antigen-containing IgG-liposomes elicit effective antitumor immunity
    Kazuko Kawamura
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Immunother 29:165-74. 2006
    ..This study demonstrates an IgG liposome with physicochemical properties suitable for delivering antigens to DCs and paves the way to the application of IgG liposomes for tumor immunotherapy using DCs...
  64. ncbi Modulation of intestinal P-glycoprotein function by polyethylene glycols and their derivatives by in vitro transport and in situ absorption studies
    Qi Shen
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Kyoto 607 8414, Japan
    Int J Pharm 313:49-56. 2006
    ..These findings suggest that PEGs and their derivatives are useful excipients to inhibit the function of intestinal P-gp, thereby improving the intestinal absorption of P-gp substrates, which are secreted by a P-gp-mediated efflux system...
  65. ncbi Effects of Labrasol and other pharmaceutical excipients on the intestinal transport and absorption of rhodamine123, a P-glycoprotein substrate, in rats
    Yulian Lin
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Kyoto, Japan
    Biol Pharm Bull 30:1301-7. 2007
    ..However, we may also consider the contribution to the enhanced intestinal absorption of rhodamine123 via a passive transport in addition to the inhibitory action of Labrasol for the function of P-gp in the intestine...
  66. ncbi Combination effects of complement regulatory proteins and anti-complement polymer
    Yasuo Yoshioka
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochim Biophys Acta 1624:54-9. 2003
    ..These data indicate that the combination of a CRP and a cytomedical device with anti-complement activity is a superior device for cytomedical therapy...
  67. ncbi Dendritic cells transduced with gp100 gene by RGD fiber-mutant adenovirus vectors are highly efficacious in generating anti-B16BL6 melanoma immunity in mice
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto, Japan
    Gene Ther 10:1891-902. 2003
    ..These findings clearly revealed that highly efficient MAA gene transduction to DCs by AdRGD could greatly improve the efficacy of DC-based immunotherapy against melanoma...
  68. ncbi Development of PEGylated adenovirus vector with targeting ligand
    Yusuke Eto
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Int J Pharm 354:3-8. 2008
    ..In this review, we describe the molecular biology of Ads and outline this PEGylation approach including our data...
  69. doi Direct cell entry of gold/iron-oxide magnetic nanoparticles in adenovirus mediated gene delivery
    Kazumasa Kamei
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan
    Biomaterials 30:1809-14. 2009
    ..This technology is a useful tool for extending Ad tropism and enhancing transduction efficiency. GoldMAN also makes possible the effective use of various biomolecules within the cell because of its interesting cell-entry mechanism...
  70. ncbi Nitric oxide donors can enhance the intestinal transport and absorption of insulin and [Asu(1,7)]-eel calcitonin in rats
    Gihan Fetih
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    J Control Release 106:287-97. 2005
    ..These results suggest that NO donors possess excellent effectiveness for the use as absorption enhancers of peptide drugs and they are very effective at lower concentrations compared to the conventional enhancers...
  71. ncbi Efficient generation of antigen-specific cellular immunity by vaccination with poly(gamma-glutamic acid) nanoparticles entrapping endoplasmic reticulum-targeted peptides
    Keisuke Matsuo
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 362:1069-72. 2007
    ..Our data provide evidence that efficient delivery of antigenic peptides into APCs, as well as active ER-translocation of antigenic peptides in APCs should be considered in the development of peptide-based cancer immunotherapy...
  72. ncbi Improvement of absorption enhancing effects of n-dodecyl-beta-D-maltopyranoside by its colon-specific delivery using chitosan capsules
    Gihan Fetih
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto, Japan
    Int J Pharm 293:127-35. 2005
    ..Therefore, chitosan capsules may be useful carriers for colon-specific delivery of LM, thereby increasing its absorption enhancing effect from the intestinal membranes...
  73. doi Creation of lysine-deficient mutant lymphotoxin-alpha with receptor selectivity by using a phage display system
    Yasuo Yoshioka
    The Center for Advanced Medical Engineering and Informatics, Osaka University, 1 6, Yamadaoka, Suita, Osaka, 565 0871, Japan
    Biomaterials 31:1935-43. 2010
    ..We also showed that the K84Q mutation contributed to the enhanced activity via TNFR1, and K39S lowered activity via TNFR2. R1selLT likely will be useful in cancer therapy and in analysis of the LT alpha structure-function relationship...
  74. ncbi [Optimization of cancer immunotherapy by controlling immune cell trafficking and biodistribution]
    Naoki Okada
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka, Suita City, Japan
    Yakugaku Zasshi 127:327-39. 2007
    ..This review introduces our approaches that employ an Arg-Gly-Asp (RGD) fiber-mutant adenovirus vector encoding the chemokine or chemokine receptor gene in cancer immunotherapy...
  75. ncbi Transport characteristics of N-acetyl-L-aspartate in rat astrocytes: involvement of sodium-coupled high-affinity carboxylate transporter NaC3/NaDC3-mediated transport system
    Takuya Fujita
    Department of Biochemical Pharmacology, Kyoto Pharmaceutical University, Jamashina ku, Kyoto, Japan
    J Neurochem 93:706-14. 2005
    ..This transporter is likely to be an essential prerequisite for the metabolic role of N-acetyl-L-aspartate in the process of myelination...
  76. ncbi Cotransduction of CCL27 gene can improve the efficacy and safety of IL-12 gene therapy for cancer
    J Q Gao
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Gene Ther 14:491-502. 2007
    ....
  77. ncbi Carrageenans can regulate the pulmonary absorption of antiasthmatic drugs and their retention in the rat lung tissues without any membrane damage
    Keigo Yamada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Int J Pharm 293:63-72. 2005
    ..Therefore, these findings indicated that the carrageenans were effective to regulate the absorption rate of antiasthmatic drugs including theophylline and fluticasone propionate...
  78. ncbi Augmentation of the migratory ability of DC-based vaccine into regional lymph nodes by efficient CCR7 gene transduction
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto, Japan
    Gene Ther 12:129-39. 2005
    ....
  79. doi Engineering of highly immunogenic long-lived DC vaccines by antiapoptotic protein gene transfer to enhance cancer vaccine potency
    T Yoshikawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Gene Ther 15:1321-9. 2008
    ..Thus, the long-lived DC vaccine strategy is highly useful for constructing DC vaccines, as well as other cell-based medicines, such as stem cell therapy...
  80. ncbi Enhanced absorption of insulin and (Asu(1,7))eel-calcitonin using novel azopolymer-coated pellets for colon-specific drug delivery
    H Tozaki
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan
    J Pharm Sci 90:89-97. 2001
    ..These findings suggest that azopolymer-coated pellets may be useful carriers for the colon-specific delivery of peptides including insulin and (Asu(1,7))eel-calcitonin...
  81. ncbi Functional characterization of brain peptide transporter in rat cerebral cortex: identification of the high-affinity type H+/peptide transporter PEPT2
    Takuya Fujita
    Department of Biochemical Pharmacology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607 8414, Japan
    Brain Res 997:52-61. 2004
    ..These results indicate that a H(+)-coupled high affinity peptide transport system is functionally expressed in the cerebral cortex and that this transport system is identical to PEPT2...
  82. ncbi Enhanced transdermal delivery of phenylalanyl-glycine by chemical modification with various fatty acids
    Akira Yamamoto
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Yamashina ku, Kyoto 607 8414, Japan
    Int J Pharm 250:119-28. 2003
    ....
  83. ncbi Control of pulmonary absorption of water-soluble compounds by various viscous vehicles
    Akira Yamamoto
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Int J Pharm 282:141-9. 2004
    ....
  84. ncbi sigma Receptor ligand-induced up-regulation of the H(+)/peptide transporter PEPT1 in the human intestinal cell line Caco-2
    T Fujita
    Department of Biopharmaceutical Sciences, Kyoto Pharmaceutical University, Yamashina ku, Kyoto, 607 8414, Japan
    Biochem Biophys Res Commun 261:242-6. 1999
    ..These data suggest that sigma(1) receptor ligand (+)pentazocine up-regulates PEPT1 in Caco-2 cells at the level of increased mRNA, causing an increase in the density of the transporter protein in the cell membrane...
  85. ncbi Interaction of kyotorphin and brain peptide transporter in synaptosomes prepared from rat cerebellum: implication of high affinity type H+/peptide transporter PEPT2 mediated transport system
    T Fujita
    Department of Biopharmaceutical Sciences, Kyoto Pharmaceutical University, Japan
    Neurosci Lett 271:117-20. 1999
    ..RT-PCR using specific primers revealed that PEPT2 mRNA exists in cerebellum in rat. Taken collectively, these results indicate that the functional peptide transport system in rat cerebellum might be the high affinity transporter PEPT2...
  86. ncbi Absorption of water-soluble compounds with different molecular weights and
    A Yamamoto
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, 607 8414, Kyoto, Japan
    J Control Release 76:363-74. 2001
    ..These findings provided useful fundamental information that might aid in the selection of administration routes for drugs of differing molecular weights including peptide drugs as far as the degree of drug absorption is concerned...
  87. pmc Administration route-dependent vaccine efficiency of murine dendritic cells pulsed with antigens
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto, 607 8414, Japan
    Br J Cancer 84:1564-70. 2001
    ..Furthermore, the immunization route affects vaccine efficiency...
  88. ncbi Evaluation of insulin permeability and effects of absorption enhancers on its permeability by an in vitro pulmonary epithelial system using Xenopus pulmonary membrane
    A Yamamoto
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Japan
    Biol Pharm Bull 24:385-9. 2001
    ....
  89. ncbi Modulation of intestinal permeability by nitric oxide donors: implications in intestinal delivery of poorly absorbable drugs
    A Yamamoto
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Kyoto, Japan
    J Pharmacol Exp Ther 296:84-90. 2001
    ..These findings suggest that NO donors may be useful to enhance the intestinal absorption of poorly absorbable drugs...
  90. ncbi [Efficient gene delivery into dendritic cells by fiber-mutant adenovirus vectors]
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan
    Yakugaku Zasshi 121:593-600. 2001
    ..We propose that alpha v-integrin-targeted FM-Ad is a very powerful tool to implement DC-based vaccination strategies...
  91. ncbi Efficient gene delivery into dendritic cells by fiber-mutant adenovirus vectors
    N Okada
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8414, Japan
    Biochem Biophys Res Commun 282:173-9. 2001
    ..We propose that alpha(v)-integrin-targeted Ad is a very powerful tool with which to implement DC-based vaccination strategies...
  92. doi Systemic administration of a PEGylated adenovirus vector with a cancer-specific promoter is effective in a mouse model of metastasis
    X Yao
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Gene Ther 16:1395-404. 2009
    ..These results suggest that modification with 20-kDa PEG at a 45% modification ratio is the optimal condition for PEGylation of Adv, and PEG-Ad-TERT/HSVtk is a prototype Adv for systemic cancer gene therapy against metastases...
  93. ncbi Cyotomedical therapy for insulinopenic diabetes using microencapsulated pancreatic beta cell lines
    Ryo Suzuki
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Life Sci 71:1717-29. 2002
    ..These results suggest that cytomedicine utilizing microencapsulated pancreatic beta cell lines with a physiological glucose sensor may be a beneficial and safe therapy with which to treat diabetes mellitus...
  94. ncbi [Development of intracellular drug delivery system using fusogenic liposomes]
    Tomoaki Yoshikawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita City, Japan
    Yakugaku Zasshi 127:789-96. 2007
    ..We suggest that this technology is very important for controlling the intracellular pharmacokinetics of biomacromolecular drugs...
  95. ncbi Chitosan capsules for colon-specific drug delivery: enhanced localization of 5-aminosalicylic acid in the large intestine accelerates healing of TNBS-induced colitis in rats
    Hideyuki Tozaki
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    J Control Release 82:51-61. 2002
    ..In conclusion, chitosan capsules may be useful carriers for the colon-specific delivery of anti-inflammatory drugs including 5-ASA and the healing of TNBS-induced colitis in rats...
  96. ncbi Modulation of intestinal P-glycoprotein function by cremophor EL and other surfactants by an in vitro diffusion chamber method using the isolated rat intestinal membranes
    Yasushi Shono
    Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi Yamashina ku, Kyoto 607 8414 Japan
    J Pharm Sci 93:877-85. 2004
    ....
  97. doi Ligand-independent assembly of purified soluble magic roundabout (Robo4), a tumor-specific endothelial marker
    Mai Yoshikawa
    Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Protein Expr Purif 61:78-82. 2008
    ..The results of these studies will help to elucidate the role of Robo4 in angiogenesis and perhaps eventually contribute to the development of novel vessel-targeting therapies...
  98. ncbi Synthesis of 15,20-triamide analogue with polar substituent on the phenyl ring of arenastatin A, an extremely potent cytotoxic spongean depsipeptide
    Naoyuki Kotoku
    Department of Natural Product Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
    Bioorg Med Chem 14:7446-57. 2006
    ..Furthermore, the diethylamine analogue (30) exhibited in vivo anti-tumor activity against subcutaneously implanted murine sarcoma...
  99. ncbi Cytotoxicity of amorphous silica particles against macrophage-like THP-1 cells depends on particle-size and surface properties
    T Morishige
    Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Pharmazie 65:596-9. 2010
    ..Our results suggest that systematic evaluation of the association between particle characteristics and biological effects is necessary for the creation of safe SPs...
  100. pmc Immune cell recruitment and cell-based system for cancer therapy
    Jian Qing Gao
    College of Pharmaceutical Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, People s Republic of China
    Pharm Res 25:752-68. 2008
    ..e., chemokines, and their receptors, and their use in cancer immunotherapy...
  101. ncbi Role of MyD88 and TLR9 in the innate immune response elicited by serotype 5 adenoviral vectors
    Tomoko Yamaguchi
    Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Osaka 567 0085, Japan
    Hum Gene Ther 18:753-62. 2007
    ..These results suggest that Ad vectors can activate innate immunity via MyD88/TLR9-dependent and -independent mechanisms...