Research Topics
| Kazuto NosakaSummaryAffiliation: Kyoto Prefectural University of Medicine Country: Japan Publications
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Detail Information
Publications
Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiaeKazuto Nosaka
Department of Chemistry, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kita ku, Kyoto 603 8334, Japan
FEBS Lett 582:3991-6. 2008..We explored the hypothesis that Thi3p causes a conformational change in Pdc2p leading to full transactivation activity under favorable conditions...
Genetic regulation mediated by thiamin pyrophosphate-binding motif in Saccharomyces cerevisiaeKazuto Nosaka
Department of Chemistry, Kyoto Prefectural University of Medicine, Kita ku, Kyoto 603 8334, Japan
Mol Microbiol 58:467-79. 2005..This is the first report of the involvement of the TDP-binding motif in genetic regulation...- Recent progress in understanding thiamin biosynthesis and its genetic regulation in Saccharomyces cerevisiaeKazuto Nosaka
Department of Chemistry, Kyoto Prefectural University of Medicine, Kita ku, Kyoto, 603 8334, Japan
Appl Microbiol Biotechnol 72:30-40. 2006..Thiamin-hyperproducing yeast and fermented food containing a high content of thiamin are expected to be available in the future based on the progress in understanding thiamin biosynthesis and its genetic regulation in S. cerevisiae... - Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiaeMari Onozuka
Department of Radioisotope Laboratory, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
FEMS Yeast Res 8:266-75. 2008..It is proposed that the thiaminase II activity of Thi20p is involved in the thiamin salvage pathway by catalyzing the hydrolysis of HMP precursors in S. cerevisiae... - Facilitated recruitment of Pdc2p, a yeast transcriptional activator, in response to thiamin starvationKazuto Nosaka
Department of Chemistry, Hyogo College of Medicine, Nishinomiya, Japan
FEMS Microbiol Lett 330:140-7. 2012..On the other hand, the association of Pdc2p with PDC5 was unaffected by thiamin. We also identified a DNA element in the upstream region of PDC5, which can bind to Pdc2p and is required for the expression of PDC5... - Molecular cloning and analysis of the 5'-flanking region of the human thiamin pyrophosphokinase geneMari Onozuka
Department of Biochemistry and Molecular Biology, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto, Japan
J Nutr Sci Vitaminol (Tokyo) 51:274-7. 2005..These findings indicate the importance of the Sp1 cis-element in regulating the hTPK1 gene expression... - Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutantKenichi Akaji
Department of Chemistry, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kita ku, Kyoto 603 8334, Japan
Bioorg Med Chem 16:9400-8. 2008..The results indicated for the first time that the tetrapeptide sequence is enough for inhibitory activities of peptide-aldehyde derivatives... - Evaluation of retro-inverso modifications of HTLV-1 protease inhibitors containing a hydroxyethylamine isosterTadashi Tatsumi
Department of Medicinal Chemistry, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kita ku, Kyoto 603 8334, Japan
Bioorg Med Chem 18:2720-7. 2010..Inhibitory activities of the new inhibitors suggest that partially modified RI inhibitors would interact with HTLV-1 protease in the same manner as the parent hydroxyethylamine inhibitor... - Synthesis of [19, 35, 36-(13)C(3)]-labeled TAK779 as a molecular probeHiroyuki Konno
Department of Chemistry, Kyoto Prefectural University of Medicine, Kita ku, Japan
Bioorg Med Chem 17:5769-74. 2009.... - NGF-dependent formation of ruffles in PC12D cells required a different pathway from that for neurite outgrowthErika Jin
Department of Biology, Kyoto Prefectural University of Medicine, Taisyogun Nishitakatsukasa cho 13, Kita ku, Kyoto 603 8334, Japan
Neurochem Int 51:216-26. 2007..Taken together, the NGF-dependent formation of ruffles might not require Ras/PI-3k/Akt/GSK-3beta, but these pathways might contribute to the formation of intact neurites due to combined actions including Rac1... - Intracellular dynamics of topoisomerase I inhibitor, CPT-11, by slit-scanning confocal Raman microscopyYoshinori Harada
Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii cho, Hirokoji Kawaramachi, Kamigyo ku, Kyoto 602 8566, Japan
Histochem Cell Biol 132:39-46. 2009..We conclude that Raman spectromicroscopic imaging is useful for pharmacokinetic studies of anticancer drugs in living cells... - Steady-state kinetics and mutational studies of recombinant human thiamin pyrophosphokinaseMari Onozuka
Department of Biochemistry, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto 602 8566, Japan
J Nutr Sci Vitaminol (Tokyo) 49:156-62. 2003..53-fold of the wild-type). It was therefore suggested that the residues Gln-96, Thr-99, Arg-131, and Asp-133 are conserved as functionally significant components for substrate recognition in thiamin pyrophosphokinase... - Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiaeYuko Kawasaki
Department of Food Microbiology, Doshisha Women s College, Kamigyo ku, Kyoto 602 0893, Japan
Curr Genet 47:156-62. 2005..We discuss the possibility that HMP is not involved in the pathway of de novo thiamin synthesis in S. cerevisiae... - Thiamine-responsive congenital lactic acidosis: clinical and biochemical studiesMitsuo Toyoshima
Division of Child Neurology, Institute of Neurological Science, Faculty of Medicine, Tottori University, Yonago, Japan
Pediatr Neurol 33:98-104. 2005..Careful monitoring of lactate and pyruvate would be useful in determining thiamine dosage...